Experiment: WM_RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt beeE and MCAODC_17050 overlap by 55 nucleotides MCAODC_17050 and MCAODC_17055 are separated by 44 nucleotides MCAODC_17055 and MCAODC_17060 overlap by 56 nucleotides MCAODC_17060 and MCAODC_17065 are separated by 4 nucleotides
MCAODC_17045: beeE - phage portal protein, at 314,457 to 315,821
beeE
MCAODC_17050: MCAODC_17050 - hypothetical protein, at 315,767 to 315,988
_17050
MCAODC_17055: MCAODC_17055 - Peptidase U35, phage prohead HK97, at 316,033 to 316,587
_17055
MCAODC_17060: MCAODC_17060 - DUF1327 domain-containing protein, at 316,532 to 316,939
_17060
MCAODC_17065: MCAODC_17065 - holin, at 316,944 to 317,150
_17065
Position (kb)
316
317 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 315.204 kb on - strand, within beeE at 315.238 kb on + strand, within beeE at 315.238 kb on + strand, within beeE at 315.239 kb on - strand, within beeE at 315.239 kb on - strand, within beeE at 315.239 kb on - strand, within beeE at 315.239 kb on - strand, within beeE at 315.239 kb on - strand, within beeE at 315.637 kb on - strand, within beeE at 316.233 kb on + strand, within MCAODC_17055 at 316.266 kb on - strand, within MCAODC_17055 at 316.266 kb on - strand, within MCAODC_17055 at 316.308 kb on - strand, within MCAODC_17055 at 316.654 kb on - strand, within MCAODC_17060 at 316.654 kb on - strand, within MCAODC_17060 at 317.325 kb on + strand at 317.326 kb on - strand at 317.372 kb on + strand at 317.372 kb on + strand at 317.372 kb on + strand at 317.373 kb on - strand at 317.390 kb on + strand at 317.390 kb on + strand at 317.390 kb on + strand at 317.390 kb on + strand at 317.391 kb on - strand at 317.391 kb on - strand at 317.391 kb on - strand at 317.543 kb on + strand at 317.545 kb on + strand at 317.545 kb on + strand at 317.545 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_RB68 remove 315,204 - beeE MCAODC_17045 0.55 -0.8 315,238 + beeE MCAODC_17045 0.57 -1.8 315,238 + beeE MCAODC_17045 0.57 +0.5 315,239 - beeE MCAODC_17045 0.57 +0.0 315,239 - beeE MCAODC_17045 0.57 +2.6 315,239 - beeE MCAODC_17045 0.57 -0.9 315,239 - beeE MCAODC_17045 0.57 -2.1 315,239 - beeE MCAODC_17045 0.57 -0.0 315,637 - beeE MCAODC_17045 0.86 +0.4 316,233 + MCAODC_17055 0.36 +0.6 316,266 - MCAODC_17055 0.42 +0.7 316,266 - MCAODC_17055 0.42 +0.2 316,308 - MCAODC_17055 0.50 -0.4 316,654 - MCAODC_17060 0.30 -2.6 316,654 - MCAODC_17060 0.30 +0.2 317,325 + -3.2 317,326 - +0.1 317,372 + -1.2 317,372 + +0.2 317,372 + -0.5 317,373 - -1.4 317,390 + -0.1 317,390 + -2.1 317,390 + +0.6 317,390 + -0.0 317,391 - -1.2 317,391 - -1.1 317,391 - +0.3 317,543 + +0.5 317,545 + +2.0 317,545 + -0.5 317,545 + +0.2
Or see this region's nucleotide sequence