Experiment: WM_RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nleG5-2 and nleG6-2 are separated by 81 nucleotides nleG6-2 and nleG2-3 are separated by 72 nucleotides nleG2-3 and MCAODC_16955 are separated by 113 nucleotides MCAODC_16955 and MCAODC_16960 are separated by 1 nucleotides
MCAODC_16940: nleG5-2 - T3SS effector NleG family protein, at 296,291 to 296,932
nleG5-2
MCAODC_16945: nleG6-2 - DUF1076 domain-containing protein, at 297,014 to 297,643
nleG6-2
MCAODC_16950: nleG2-3 - DUF1076 domain-containing protein, at 297,716 to 298,291
nleG2-3
MCAODC_16955: MCAODC_16955 - phage tail protein, at 298,405 to 298,674
_16955
MCAODC_16960: MCAODC_16960 - Tail fiber protein, at 298,676 to 299,989
_16960
Position (kb) 297
298
299 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 297.155 kb on + strand, within nleG6-2 at 298.055 kb on + strand, within nleG2-3 at 298.091 kb on - strand, within nleG2-3 at 298.157 kb on - strand, within nleG2-3 at 298.327 kb on + strand at 298.327 kb on + strand at 298.331 kb on + strand at 298.332 kb on - strand at 298.337 kb on + strand at 298.353 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_RB68 remove 297,155 + nleG6-2 MCAODC_16945 0.22 -0.9 298,055 + nleG2-3 MCAODC_16950 0.59 +0.6 298,091 - nleG2-3 MCAODC_16950 0.65 +1.2 298,157 - nleG2-3 MCAODC_16950 0.77 -0.0 298,327 + +1.6 298,327 + -0.2 298,331 + +0.5 298,332 - -0.0 298,337 + -2.9 298,353 + +0.5
Or see this region's nucleotide sequence