Strain Fitness in Escherichia coli ECRC101 around MCAODC_16400

Experiment: WM_RB68

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpatD and ortT are separated by 386 nucleotidesortT and ydcY are separated by 85 nucleotidesydcY and MCAODC_16400 are separated by 0 nucleotidesMCAODC_16400 and mddA overlap by 4 nucleotidesmddA and curA are separated by 180 nucleotides MCAODC_16385: patD - aminobutyraldehyde dehydrogenase, at 168,905 to 170,329 patD MCAODC_16390: ortT - orphan toxin OrtT, at 170,716 to 170,889 ortT MCAODC_16395: ydcY - Uncharacterized protein YdcY, at 170,975 to 171,208 ydcY MCAODC_16400: MCAODC_16400 - Putative inner membrane exporter, YdcZ, at 171,209 to 171,658 _16400 MCAODC_16405: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 171,655 to 172,173 mddA MCAODC_16410: curA - NADPH-dependent curcumin/dihydrocurcumin reductase, at 172,354 to 173,391 curA Position (kb) 171 172Strain fitness (log2 ratio) -2 -1 0 1 2 3at 170.220 kb on + strandat 170.270 kb on - strandat 170.328 kb on - strandat 170.349 kb on - strandat 170.456 kb on - strandat 170.456 kb on - strandat 170.456 kb on - strandat 170.456 kb on - strandat 170.456 kb on - strandat 170.490 kb on - strandat 170.495 kb on + strandat 170.496 kb on - strandat 170.498 kb on + strandat 170.498 kb on + strandat 170.499 kb on - strandat 170.499 kb on - strandat 170.548 kb on - strandat 170.554 kb on - strandat 170.554 kb on - strandat 170.556 kb on - strandat 170.556 kb on - strandat 170.556 kb on - strandat 170.559 kb on - strandat 170.583 kb on + strandat 170.927 kb on + strandat 171.051 kb on + strand, within ydcYat 171.098 kb on + strand, within ydcYat 171.151 kb on + strand, within ydcYat 171.151 kb on + strand, within ydcYat 171.151 kb on + strand, within ydcYat 171.151 kb on + strand, within ydcYat 171.152 kb on - strand, within ydcYat 171.361 kb on + strand, within MCAODC_16400at 171.362 kb on - strand, within MCAODC_16400at 171.435 kb on - strand, within MCAODC_16400at 171.437 kb on - strand, within MCAODC_16400at 171.437 kb on - strand, within MCAODC_16400at 171.437 kb on - strand, within MCAODC_16400at 171.485 kb on - strand, within MCAODC_16400at 171.703 kb on - strandat 172.009 kb on + strand, within mddAat 172.031 kb on - strand, within mddAat 172.102 kb on - strand, within mddAat 172.103 kb on + strand, within mddAat 172.103 kb on + strand, within mddAat 172.192 kb on - strandat 172.192 kb on - strandat 172.192 kb on - strandat 172.212 kb on + strandat 172.212 kb on + strandat 172.318 kb on - strandat 172.479 kb on - strand, within curAat 172.510 kb on + strand, within curAat 172.510 kb on + strand, within curAat 172.511 kb on - strand, within curAat 172.512 kb on + strand, within curAat 172.512 kb on + strand, within curAat 172.512 kb on + strand, within curAat 172.524 kb on + strand, within curAat 172.524 kb on + strand, within curAat 172.524 kb on + strand, within curAat 172.543 kb on + strand, within curAat 172.544 kb on - strand, within curA

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_RB68
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170,220 + +1.0
170,270 - -0.0
170,328 - -0.4
170,349 - +1.0
170,456 - +0.6
170,456 - -1.9
170,456 - +0.5
170,456 - +1.3
170,456 - -0.2
170,490 - -0.4
170,495 + +0.8
170,496 - -0.2
170,498 + -0.6
170,498 + -0.7
170,499 - +0.2
170,499 - +0.6
170,548 - +0.7
170,554 - -0.1
170,554 - -0.3
170,556 - +1.0
170,556 - +0.2
170,556 - +0.3
170,559 - +2.2
170,583 + -1.6
170,927 + -1.6
171,051 + ydcY MCAODC_16395 0.32 +0.9
171,098 + ydcY MCAODC_16395 0.53 -0.4
171,151 + ydcY MCAODC_16395 0.75 -1.4
171,151 + ydcY MCAODC_16395 0.75 -2.0
171,151 + ydcY MCAODC_16395 0.75 +0.4
171,151 + ydcY MCAODC_16395 0.75 +0.6
171,152 - ydcY MCAODC_16395 0.76 -0.6
171,361 + MCAODC_16400 0.34 +1.5
171,362 - MCAODC_16400 0.34 -0.3
171,435 - MCAODC_16400 0.50 +0.4
171,437 - MCAODC_16400 0.51 -0.0
171,437 - MCAODC_16400 0.51 -0.1
171,437 - MCAODC_16400 0.51 +0.3
171,485 - MCAODC_16400 0.61 +0.9
171,703 - -0.0
172,009 + mddA MCAODC_16405 0.68 +0.2
172,031 - mddA MCAODC_16405 0.72 +0.2
172,102 - mddA MCAODC_16405 0.86 +1.2
172,103 + mddA MCAODC_16405 0.86 +0.5
172,103 + mddA MCAODC_16405 0.86 +0.6
172,192 - +0.8
172,192 - +0.6
172,192 - -1.8
172,212 + -1.5
172,212 + +0.7
172,318 - -0.9
172,479 - curA MCAODC_16410 0.12 +0.7
172,510 + curA MCAODC_16410 0.15 +1.5
172,510 + curA MCAODC_16410 0.15 -1.8
172,511 - curA MCAODC_16410 0.15 +1.0
172,512 + curA MCAODC_16410 0.15 +0.1
172,512 + curA MCAODC_16410 0.15 +0.1
172,512 + curA MCAODC_16410 0.15 +0.8
172,524 + curA MCAODC_16410 0.16 +1.3
172,524 + curA MCAODC_16410 0.16 +3.1
172,524 + curA MCAODC_16410 0.16 -0.1
172,543 + curA MCAODC_16410 0.18 +1.9
172,544 - curA MCAODC_16410 0.18 -0.4

Or see this region's nucleotide sequence