Experiment: WM_RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hybC and hybD overlap by 1 nucleotides hybD and hybE overlap by 8 nucleotides hybE and hypA overlap by 8 nucleotides hypA and hybG are separated by 12 nucleotides hybG and yghU are separated by 122 nucleotides
MCAODC_09535: hybC - hydrogenase 2 large subunit, at 1,901,425 to 1,903,128
hybC
MCAODC_09540: hybD - HyaD/HybD family hydrogenase maturation endopeptidase, at 1,903,128 to 1,903,622
hybD
MCAODC_09545: hybE - hydrogenase-2 assembly chaperone, at 1,903,615 to 1,904,103
hybE
MCAODC_09550: hypA - hydrogenase maturation nickel metallochaperone HypA, at 1,904,096 to 1,904,437
hypA
MCAODC_09555: hybG - hydrogenase maturation factor HybG, at 1,904,450 to 1,904,698
hybG
MCAODC_09565: yghU - glutathione-dependent disulfide-bond oxidoreductase, at 1,904,821 to 1,905,687
yghU
Position (kb)
1904
1905 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1903.133 kb on + strand at 1903.134 kb on - strand at 1903.138 kb on + strand at 1903.157 kb on + strand at 1903.215 kb on + strand, within hybD at 1903.215 kb on + strand, within hybD at 1903.216 kb on - strand, within hybD at 1903.216 kb on - strand, within hybD at 1903.216 kb on - strand, within hybD at 1903.216 kb on - strand, within hybD at 1903.216 kb on - strand, within hybD at 1903.230 kb on + strand, within hybD at 1903.230 kb on + strand, within hybD at 1903.483 kb on + strand, within hybD at 1903.628 kb on + strand at 1903.629 kb on - strand at 1903.662 kb on - strand at 1903.662 kb on - strand at 1903.735 kb on + strand, within hybE at 1903.735 kb on + strand, within hybE at 1903.736 kb on - strand, within hybE at 1903.809 kb on + strand, within hybE at 1903.809 kb on + strand, within hybE at 1903.880 kb on - strand, within hybE at 1903.927 kb on + strand, within hybE at 1903.965 kb on + strand, within hybE at 1903.965 kb on + strand, within hybE at 1903.966 kb on - strand, within hybE at 1904.165 kb on - strand, within hypA at 1904.233 kb on + strand, within hypA at 1904.280 kb on + strand, within hypA at 1904.280 kb on + strand, within hypA at 1904.280 kb on + strand, within hypA at 1904.281 kb on - strand, within hypA at 1904.281 kb on - strand, within hypA at 1904.284 kb on + strand, within hypA at 1904.284 kb on + strand, within hypA at 1904.284 kb on + strand, within hypA at 1904.285 kb on - strand, within hypA at 1904.285 kb on - strand, within hypA at 1904.291 kb on + strand, within hypA at 1904.422 kb on + strand at 1904.422 kb on + strand at 1904.422 kb on + strand at 1904.423 kb on - strand at 1904.445 kb on + strand at 1904.455 kb on + strand at 1904.455 kb on + strand at 1904.455 kb on + strand at 1904.456 kb on - strand at 1904.494 kb on + strand, within hybG at 1904.495 kb on - strand, within hybG at 1904.520 kb on + strand, within hybG at 1904.520 kb on + strand, within hybG at 1904.520 kb on + strand, within hybG at 1904.520 kb on + strand, within hybG at 1904.520 kb on + strand, within hybG at 1904.521 kb on - strand, within hybG at 1904.521 kb on - strand, within hybG at 1904.521 kb on - strand, within hybG at 1904.521 kb on - strand, within hybG at 1904.567 kb on - strand, within hybG at 1904.679 kb on - strand at 1904.713 kb on - strand at 1904.836 kb on - strand at 1904.836 kb on - strand at 1904.836 kb on - strand at 1904.979 kb on + strand, within yghU at 1904.980 kb on - strand, within yghU at 1904.980 kb on - strand, within yghU at 1904.980 kb on - strand, within yghU at 1904.982 kb on - strand, within yghU at 1904.982 kb on - strand, within yghU at 1905.014 kb on + strand, within yghU at 1905.014 kb on + strand, within yghU at 1905.015 kb on - strand, within yghU at 1905.015 kb on - strand, within yghU at 1905.015 kb on - strand, within yghU at 1905.074 kb on + strand, within yghU at 1905.074 kb on + strand, within yghU at 1905.074 kb on + strand, within yghU at 1905.074 kb on + strand, within yghU at 1905.074 kb on + strand, within yghU at 1905.075 kb on - strand, within yghU at 1905.075 kb on - strand, within yghU at 1905.096 kb on + strand, within yghU at 1905.096 kb on + strand, within yghU at 1905.096 kb on + strand, within yghU at 1905.096 kb on + strand, within yghU at 1905.097 kb on - strand, within yghU at 1905.097 kb on - strand, within yghU at 1905.150 kb on + strand, within yghU at 1905.194 kb on + strand, within yghU at 1905.297 kb on - strand, within yghU at 1905.318 kb on - strand, within yghU at 1905.360 kb on + strand, within yghU at 1905.366 kb on + strand, within yghU
Per-strain Table
Position Strand Gene LocusTag Fraction WM_RB68 remove 1,903,133 + +1.9 1,903,134 - -0.5 1,903,138 + -2.3 1,903,157 + +0.6 1,903,215 + hybD MCAODC_09540 0.18 -0.1 1,903,215 + hybD MCAODC_09540 0.18 -1.4 1,903,216 - hybD MCAODC_09540 0.18 -0.4 1,903,216 - hybD MCAODC_09540 0.18 +1.9 1,903,216 - hybD MCAODC_09540 0.18 -0.7 1,903,216 - hybD MCAODC_09540 0.18 +0.4 1,903,216 - hybD MCAODC_09540 0.18 -0.8 1,903,230 + hybD MCAODC_09540 0.21 +0.2 1,903,230 + hybD MCAODC_09540 0.21 -0.4 1,903,483 + hybD MCAODC_09540 0.72 +0.6 1,903,628 + +0.6 1,903,629 - +1.2 1,903,662 - -1.0 1,903,662 - -0.5 1,903,735 + hybE MCAODC_09545 0.25 +1.5 1,903,735 + hybE MCAODC_09545 0.25 +0.5 1,903,736 - hybE MCAODC_09545 0.25 -0.2 1,903,809 + hybE MCAODC_09545 0.40 -0.7 1,903,809 + hybE MCAODC_09545 0.40 -0.1 1,903,880 - hybE MCAODC_09545 0.54 -0.4 1,903,927 + hybE MCAODC_09545 0.64 -3.3 1,903,965 + hybE MCAODC_09545 0.72 -1.4 1,903,965 + hybE MCAODC_09545 0.72 -0.1 1,903,966 - hybE MCAODC_09545 0.72 -0.2 1,904,165 - hypA MCAODC_09550 0.20 -0.3 1,904,233 + hypA MCAODC_09550 0.40 -0.1 1,904,280 + hypA MCAODC_09550 0.54 +1.0 1,904,280 + hypA MCAODC_09550 0.54 +0.0 1,904,280 + hypA MCAODC_09550 0.54 -1.0 1,904,281 - hypA MCAODC_09550 0.54 +0.3 1,904,281 - hypA MCAODC_09550 0.54 +0.4 1,904,284 + hypA MCAODC_09550 0.55 -0.8 1,904,284 + hypA MCAODC_09550 0.55 -2.6 1,904,284 + hypA MCAODC_09550 0.55 -0.3 1,904,285 - hypA MCAODC_09550 0.55 +0.2 1,904,285 - hypA MCAODC_09550 0.55 +1.3 1,904,291 + hypA MCAODC_09550 0.57 -1.0 1,904,422 + -1.5 1,904,422 + +1.4 1,904,422 + +0.3 1,904,423 - -0.8 1,904,445 + +0.2 1,904,455 + +1.8 1,904,455 + +0.6 1,904,455 + +0.5 1,904,456 - -1.5 1,904,494 + hybG MCAODC_09555 0.18 -2.4 1,904,495 - hybG MCAODC_09555 0.18 +0.6 1,904,520 + hybG MCAODC_09555 0.28 +0.4 1,904,520 + hybG MCAODC_09555 0.28 +0.3 1,904,520 + hybG MCAODC_09555 0.28 +0.4 1,904,520 + hybG MCAODC_09555 0.28 +0.1 1,904,520 + hybG MCAODC_09555 0.28 +0.0 1,904,521 - hybG MCAODC_09555 0.29 -0.7 1,904,521 - hybG MCAODC_09555 0.29 +0.7 1,904,521 - hybG MCAODC_09555 0.29 +1.1 1,904,521 - hybG MCAODC_09555 0.29 +0.6 1,904,567 - hybG MCAODC_09555 0.47 +1.8 1,904,679 - +1.7 1,904,713 - +0.0 1,904,836 - -0.5 1,904,836 - +0.4 1,904,836 - -0.0 1,904,979 + yghU MCAODC_09565 0.18 +0.2 1,904,980 - yghU MCAODC_09565 0.18 +0.6 1,904,980 - yghU MCAODC_09565 0.18 +0.7 1,904,980 - yghU MCAODC_09565 0.18 +0.6 1,904,982 - yghU MCAODC_09565 0.19 +0.1 1,904,982 - yghU MCAODC_09565 0.19 -0.2 1,905,014 + yghU MCAODC_09565 0.22 -0.1 1,905,014 + yghU MCAODC_09565 0.22 -0.0 1,905,015 - yghU MCAODC_09565 0.22 +0.2 1,905,015 - yghU MCAODC_09565 0.22 +0.9 1,905,015 - yghU MCAODC_09565 0.22 +0.6 1,905,074 + yghU MCAODC_09565 0.29 +1.0 1,905,074 + yghU MCAODC_09565 0.29 +0.4 1,905,074 + yghU MCAODC_09565 0.29 -0.5 1,905,074 + yghU MCAODC_09565 0.29 +2.2 1,905,074 + yghU MCAODC_09565 0.29 +0.5 1,905,075 - yghU MCAODC_09565 0.29 -0.0 1,905,075 - yghU MCAODC_09565 0.29 -0.1 1,905,096 + yghU MCAODC_09565 0.32 -0.0 1,905,096 + yghU MCAODC_09565 0.32 -0.9 1,905,096 + yghU MCAODC_09565 0.32 +0.5 1,905,096 + yghU MCAODC_09565 0.32 +0.7 1,905,097 - yghU MCAODC_09565 0.32 +0.6 1,905,097 - yghU MCAODC_09565 0.32 +1.1 1,905,150 + yghU MCAODC_09565 0.38 -0.3 1,905,194 + yghU MCAODC_09565 0.43 +0.5 1,905,297 - yghU MCAODC_09565 0.55 +0.2 1,905,318 - yghU MCAODC_09565 0.57 -0.2 1,905,360 + yghU MCAODC_09565 0.62 +1.1 1,905,366 + yghU MCAODC_09565 0.63 -0.2
Or see this region's nucleotide sequence