Experiment: WM_RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fusA and tufA_E379K are separated by 70 nucleotides tufA_E379K and bfd are separated by 182 nucleotides bfd and bfr are separated by 72 nucleotides bfr and pulO overlap by 4 nucleotides
MCAODC_07860: fusA - elongation factor G, at 1,592,775 to 1,594,889
fusA
MCAODC_07865: tufA_E379K - translation elongation factor EF-Tu 1, at 1,594,960 to 1,596,144
_E379K
MCAODC_07870: bfd - bacterioferritin-associated ferredoxin, at 1,596,327 to 1,596,521
bfd
MCAODC_07875: bfr - bacterioferritin, at 1,596,594 to 1,597,070
bfr
MCAODC_07880: pulO - prepilin peptidase, at 1,597,067 to 1,597,534
pulO
Position (kb)
1594
1595
1596
1597 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1594.941 kb on + strand at 1594.941 kb on + strand at 1594.942 kb on - strand at 1594.943 kb on + strand at 1594.943 kb on + strand at 1594.943 kb on + strand at 1595.554 kb on + strand, within tufA_E379K at 1595.555 kb on - strand, within tufA_E379K at 1596.142 kb on + strand at 1596.146 kb on + strand at 1596.146 kb on + strand at 1596.146 kb on + strand at 1596.147 kb on - strand at 1596.153 kb on + strand at 1596.153 kb on + strand at 1596.158 kb on - strand at 1596.219 kb on + strand at 1596.223 kb on + strand at 1596.247 kb on + strand at 1596.248 kb on - strand at 1596.248 kb on - strand at 1596.258 kb on + strand at 1596.259 kb on - strand at 1596.281 kb on - strand at 1596.330 kb on + strand at 1596.331 kb on - strand at 1596.331 kb on - strand at 1596.331 kb on - strand at 1596.344 kb on + strand at 1596.345 kb on - strand at 1596.345 kb on - strand at 1596.352 kb on + strand, within bfd at 1596.352 kb on + strand, within bfd at 1596.533 kb on - strand at 1596.560 kb on + strand at 1596.560 kb on + strand at 1596.561 kb on - strand at 1596.567 kb on + strand at 1596.567 kb on + strand at 1596.567 kb on + strand at 1596.567 kb on + strand at 1596.568 kb on - strand at 1596.568 kb on - strand at 1596.570 kb on + strand at 1596.570 kb on + strand at 1596.571 kb on - strand at 1596.589 kb on - strand at 1596.589 kb on - strand at 1596.590 kb on + strand at 1596.634 kb on + strand at 1596.634 kb on + strand at 1596.635 kb on - strand at 1596.666 kb on + strand, within bfr at 1596.713 kb on + strand, within bfr at 1596.713 kb on + strand, within bfr at 1596.713 kb on + strand, within bfr at 1596.714 kb on - strand, within bfr at 1596.714 kb on - strand, within bfr at 1596.727 kb on - strand, within bfr at 1596.762 kb on - strand, within bfr at 1596.762 kb on - strand, within bfr at 1596.765 kb on + strand, within bfr at 1596.765 kb on + strand, within bfr at 1596.765 kb on + strand, within bfr at 1596.766 kb on - strand, within bfr at 1596.806 kb on - strand, within bfr at 1596.818 kb on + strand, within bfr at 1596.819 kb on - strand, within bfr at 1596.819 kb on - strand, within bfr at 1596.819 kb on - strand, within bfr at 1596.824 kb on - strand, within bfr at 1596.871 kb on + strand, within bfr at 1596.933 kb on + strand, within bfr at 1596.933 kb on + strand, within bfr at 1596.933 kb on + strand, within bfr at 1596.933 kb on + strand, within bfr at 1596.934 kb on - strand, within bfr at 1596.934 kb on - strand, within bfr at 1596.934 kb on - strand, within bfr at 1596.934 kb on - strand, within bfr at 1597.056 kb on - strand at 1597.077 kb on - strand at 1597.088 kb on - strand at 1597.097 kb on + strand at 1597.097 kb on + strand at 1597.098 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_RB68 remove 1,594,941 + +1.6 1,594,941 + +1.0 1,594,942 - -1.0 1,594,943 + -0.2 1,594,943 + -2.1 1,594,943 + -3.3 1,595,554 + tufA_E379K MCAODC_07865 0.50 +1.2 1,595,555 - tufA_E379K MCAODC_07865 0.50 -1.2 1,596,142 + +0.0 1,596,146 + +1.6 1,596,146 + +0.8 1,596,146 + -1.5 1,596,147 - +1.2 1,596,153 + -0.9 1,596,153 + +0.5 1,596,158 - -0.4 1,596,219 + +1.2 1,596,223 + +0.1 1,596,247 + +0.1 1,596,248 - +0.1 1,596,248 - -0.1 1,596,258 + +0.9 1,596,259 - +0.3 1,596,281 - +0.6 1,596,330 + -0.4 1,596,331 - -1.2 1,596,331 - -1.4 1,596,331 - +0.0 1,596,344 + -1.5 1,596,345 - -0.1 1,596,345 - -0.4 1,596,352 + bfd MCAODC_07870 0.13 +0.3 1,596,352 + bfd MCAODC_07870 0.13 +0.4 1,596,533 - -0.1 1,596,560 + -0.0 1,596,560 + -0.4 1,596,561 - +0.9 1,596,567 + -0.9 1,596,567 + -0.2 1,596,567 + +0.0 1,596,567 + -3.4 1,596,568 - +0.7 1,596,568 - -0.5 1,596,570 + -0.1 1,596,570 + -0.6 1,596,571 - +0.2 1,596,589 - +0.9 1,596,589 - -0.9 1,596,590 + +1.7 1,596,634 + -2.3 1,596,634 + -0.1 1,596,635 - +0.4 1,596,666 + bfr MCAODC_07875 0.15 +0.7 1,596,713 + bfr MCAODC_07875 0.25 -0.6 1,596,713 + bfr MCAODC_07875 0.25 +0.9 1,596,713 + bfr MCAODC_07875 0.25 +2.5 1,596,714 - bfr MCAODC_07875 0.25 -0.5 1,596,714 - bfr MCAODC_07875 0.25 +0.9 1,596,727 - bfr MCAODC_07875 0.28 +0.1 1,596,762 - bfr MCAODC_07875 0.35 +0.1 1,596,762 - bfr MCAODC_07875 0.35 +0.9 1,596,765 + bfr MCAODC_07875 0.36 +0.1 1,596,765 + bfr MCAODC_07875 0.36 -1.0 1,596,765 + bfr MCAODC_07875 0.36 -0.3 1,596,766 - bfr MCAODC_07875 0.36 -1.0 1,596,806 - bfr MCAODC_07875 0.44 -0.5 1,596,818 + bfr MCAODC_07875 0.47 -1.8 1,596,819 - bfr MCAODC_07875 0.47 -0.2 1,596,819 - bfr MCAODC_07875 0.47 +0.5 1,596,819 - bfr MCAODC_07875 0.47 -0.2 1,596,824 - bfr MCAODC_07875 0.48 +0.0 1,596,871 + bfr MCAODC_07875 0.58 -1.0 1,596,933 + bfr MCAODC_07875 0.71 -0.2 1,596,933 + bfr MCAODC_07875 0.71 -0.8 1,596,933 + bfr MCAODC_07875 0.71 -0.8 1,596,933 + bfr MCAODC_07875 0.71 +1.2 1,596,934 - bfr MCAODC_07875 0.71 -0.8 1,596,934 - bfr MCAODC_07875 0.71 +0.7 1,596,934 - bfr MCAODC_07875 0.71 -0.2 1,596,934 - bfr MCAODC_07875 0.71 -1.9 1,597,056 - +0.2 1,597,077 - -2.4 1,597,088 - +0.3 1,597,097 + +0.8 1,597,097 + +0.8 1,597,098 - +0.1
Or see this region's nucleotide sequence