Experiment: WM_RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cra and fruL are separated by 81 nucleotides fruL and ilvN are separated by 11 nucleotides ilvN and ilvI are separated by 2 nucleotides ilvI and leuO are separated by 317 nucleotides
MCAODC_01200: cra - catabolite repressor/activator, at 223,573 to 224,577
cra
MCAODC_01205: fruL - Putative fruR/shl operon leader peptide, at 224,659 to 224,745
fruL
MCAODC_01210: ilvN - acetolactate synthase small subunit, at 224,757 to 225,248
ilvN
MCAODC_01215: ilvI - acetolactate synthase 3 large subunit, at 225,251 to 226,975
ilvI
MCAODC_01220: leuO - transcriptional regulator LeuO, at 227,293 to 228,237
leuO
Position (kb) 225
226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 224.303 kb on + strand, within cra at 224.333 kb on + strand, within cra at 224.334 kb on - strand, within cra at 224.334 kb on - strand, within cra at 224.337 kb on + strand, within cra at 224.352 kb on - strand, within cra at 224.388 kb on + strand, within cra at 224.388 kb on + strand, within cra at 224.388 kb on + strand, within cra at 224.389 kb on - strand, within cra at 224.438 kb on + strand, within cra at 224.438 kb on + strand, within cra at 224.439 kb on - strand, within cra at 224.439 kb on - strand, within cra at 224.495 kb on + strand at 224.495 kb on + strand at 224.517 kb on + strand at 224.517 kb on + strand at 224.518 kb on - strand at 224.518 kb on - strand at 224.522 kb on + strand at 224.522 kb on + strand at 224.522 kb on + strand at 224.523 kb on - strand at 224.523 kb on - strand at 224.523 kb on - strand at 224.523 kb on - strand at 224.593 kb on - strand at 224.593 kb on - strand at 224.646 kb on + strand at 224.646 kb on + strand at 224.647 kb on - strand at 224.647 kb on - strand at 224.647 kb on - strand at 224.680 kb on - strand, within fruL at 224.771 kb on + strand at 224.844 kb on + strand, within ilvN at 224.877 kb on + strand, within ilvN at 224.878 kb on - strand, within ilvN at 224.878 kb on - strand, within ilvN at 224.888 kb on + strand, within ilvN at 224.890 kb on + strand, within ilvN at 224.891 kb on - strand, within ilvN at 224.913 kb on - strand, within ilvN at 224.939 kb on + strand, within ilvN at 224.939 kb on + strand, within ilvN at 224.940 kb on - strand, within ilvN at 224.946 kb on - strand, within ilvN at 224.946 kb on - strand, within ilvN at 225.055 kb on + strand, within ilvN at 225.055 kb on + strand, within ilvN at 225.055 kb on + strand, within ilvN at 225.055 kb on + strand, within ilvN at 225.056 kb on - strand, within ilvN at 225.056 kb on - strand, within ilvN at 225.057 kb on + strand, within ilvN at 225.057 kb on + strand, within ilvN at 225.057 kb on + strand, within ilvN at 225.058 kb on - strand, within ilvN at 225.058 kb on - strand, within ilvN at 225.058 kb on - strand, within ilvN at 225.058 kb on - strand, within ilvN at 225.058 kb on - strand, within ilvN at 225.113 kb on + strand, within ilvN at 225.113 kb on + strand, within ilvN at 225.114 kb on - strand, within ilvN at 225.167 kb on - strand, within ilvN at 225.167 kb on - strand, within ilvN at 225.235 kb on - strand at 225.367 kb on + strand at 225.368 kb on - strand at 225.392 kb on - strand at 225.444 kb on + strand, within ilvI at 225.445 kb on - strand, within ilvI at 225.445 kb on - strand, within ilvI at 225.474 kb on - strand, within ilvI at 225.484 kb on + strand, within ilvI at 225.484 kb on + strand, within ilvI at 225.485 kb on - strand, within ilvI at 225.485 kb on - strand, within ilvI at 225.485 kb on - strand, within ilvI at 225.486 kb on + strand, within ilvI at 225.486 kb on + strand, within ilvI at 225.486 kb on + strand, within ilvI at 225.486 kb on + strand, within ilvI at 225.486 kb on + strand, within ilvI at 225.487 kb on - strand, within ilvI at 225.487 kb on - strand, within ilvI at 225.487 kb on - strand, within ilvI at 225.487 kb on - strand, within ilvI at 225.487 kb on - strand, within ilvI at 225.487 kb on - strand, within ilvI at 225.487 kb on - strand, within ilvI at 225.487 kb on - strand, within ilvI at 225.510 kb on + strand, within ilvI at 225.511 kb on - strand, within ilvI at 225.511 kb on - strand, within ilvI at 225.547 kb on - strand, within ilvI at 225.556 kb on + strand, within ilvI at 225.556 kb on + strand, within ilvI at 225.556 kb on + strand, within ilvI at 225.557 kb on - strand, within ilvI at 225.586 kb on - strand, within ilvI at 225.586 kb on - strand, within ilvI at 225.709 kb on + strand, within ilvI at 225.710 kb on - strand, within ilvI at 225.759 kb on + strand, within ilvI at 225.762 kb on + strand, within ilvI at 225.762 kb on + strand, within ilvI at 225.763 kb on - strand, within ilvI at 225.802 kb on + strand, within ilvI at 225.803 kb on - strand, within ilvI at 225.807 kb on + strand, within ilvI at 225.882 kb on + strand, within ilvI at 225.883 kb on - strand, within ilvI at 226.054 kb on + strand, within ilvI at 226.054 kb on + strand, within ilvI at 226.054 kb on + strand, within ilvI at 226.055 kb on - strand, within ilvI at 226.055 kb on - strand, within ilvI at 226.095 kb on + strand, within ilvI at 226.096 kb on - strand, within ilvI at 226.163 kb on - strand, within ilvI at 226.175 kb on - strand, within ilvI at 226.186 kb on - strand, within ilvI at 226.186 kb on - strand, within ilvI at 226.186 kb on - strand, within ilvI at 226.190 kb on - strand, within ilvI at 226.337 kb on + strand, within ilvI at 226.338 kb on - strand, within ilvI at 226.338 kb on - strand, within ilvI at 226.341 kb on + strand, within ilvI at 226.341 kb on + strand, within ilvI at 226.342 kb on - strand, within ilvI at 226.399 kb on + strand, within ilvI at 226.400 kb on - strand, within ilvI at 226.400 kb on - strand, within ilvI at 226.400 kb on - strand, within ilvI at 226.411 kb on + strand, within ilvI at 226.411 kb on + strand, within ilvI at 226.412 kb on - strand, within ilvI at 226.412 kb on - strand, within ilvI at 226.422 kb on + strand, within ilvI at 226.422 kb on + strand, within ilvI at 226.423 kb on - strand, within ilvI at 226.455 kb on + strand, within ilvI at 226.455 kb on + strand, within ilvI at 226.455 kb on + strand, within ilvI at 226.456 kb on - strand, within ilvI at 226.477 kb on + strand, within ilvI at 226.483 kb on + strand, within ilvI at 226.483 kb on + strand, within ilvI at 226.483 kb on + strand, within ilvI at 226.647 kb on + strand, within ilvI at 226.648 kb on - strand, within ilvI at 226.653 kb on - strand, within ilvI at 226.688 kb on + strand, within ilvI at 226.688 kb on + strand, within ilvI at 226.688 kb on + strand, within ilvI at 226.688 kb on + strand, within ilvI at 226.689 kb on - strand, within ilvI at 226.705 kb on + strand, within ilvI at 226.705 kb on + strand, within ilvI at 226.706 kb on - strand, within ilvI at 226.706 kb on - strand, within ilvI at 226.706 kb on - strand, within ilvI at 226.706 kb on - strand, within ilvI at 226.707 kb on + strand, within ilvI at 226.707 kb on + strand, within ilvI at 226.708 kb on - strand, within ilvI at 226.708 kb on - strand, within ilvI at 226.757 kb on + strand, within ilvI at 226.807 kb on + strand at 226.807 kb on + strand at 226.808 kb on - strand at 226.836 kb on - strand at 226.875 kb on + strand at 226.875 kb on + strand at 226.876 kb on - strand at 226.876 kb on - strand at 226.876 kb on - strand at 226.876 kb on - strand at 226.910 kb on + strand at 226.911 kb on - strand at 226.919 kb on - strand at 227.019 kb on - strand at 227.060 kb on - strand at 227.060 kb on - strand at 227.073 kb on + strand at 227.073 kb on + strand at 227.121 kb on + strand at 227.139 kb on + strand at 227.165 kb on + strand at 227.202 kb on + strand at 227.279 kb on + strand at 227.314 kb on + strand at 227.344 kb on - strand at 227.375 kb on + strand at 227.375 kb on + strand at 227.376 kb on - strand at 227.376 kb on - strand at 227.389 kb on - strand, within leuO at 227.414 kb on - strand, within leuO at 227.419 kb on + strand, within leuO at 227.419 kb on + strand, within leuO at 227.419 kb on + strand, within leuO at 227.496 kb on + strand, within leuO at 227.497 kb on - strand, within leuO at 227.497 kb on - strand, within leuO at 227.500 kb on + strand, within leuO at 227.500 kb on + strand, within leuO at 227.501 kb on - strand, within leuO at 227.501 kb on - strand, within leuO at 227.501 kb on - strand, within leuO at 227.544 kb on + strand, within leuO at 227.545 kb on - strand, within leuO at 227.545 kb on - strand, within leuO at 227.545 kb on - strand, within leuO at 227.545 kb on - strand, within leuO at 227.548 kb on + strand, within leuO at 227.548 kb on + strand, within leuO at 227.571 kb on + strand, within leuO at 227.616 kb on + strand, within leuO at 227.671 kb on + strand, within leuO at 227.696 kb on - strand, within leuO at 227.736 kb on - strand, within leuO at 227.739 kb on + strand, within leuO at 227.740 kb on - strand, within leuO at 227.740 kb on - strand, within leuO at 227.791 kb on + strand, within leuO at 227.808 kb on + strand, within leuO at 227.808 kb on + strand, within leuO at 227.809 kb on - strand, within leuO at 227.809 kb on - strand, within leuO at 227.809 kb on - strand, within leuO at 227.815 kb on + strand, within leuO at 227.815 kb on + strand, within leuO at 227.815 kb on + strand, within leuO at 227.816 kb on - strand, within leuO at 227.817 kb on + strand, within leuO at 227.818 kb on - strand, within leuO at 227.818 kb on - strand, within leuO at 227.844 kb on + strand, within leuO at 227.899 kb on + strand, within leuO at 227.899 kb on + strand, within leuO at 227.953 kb on + strand, within leuO
Per-strain Table
Position Strand Gene LocusTag Fraction WM_RB68 remove 224,303 + cra MCAODC_01200 0.73 +1.3 224,333 + cra MCAODC_01200 0.76 -0.4 224,334 - cra MCAODC_01200 0.76 +1.4 224,334 - cra MCAODC_01200 0.76 -1.4 224,337 + cra MCAODC_01200 0.76 +1.0 224,352 - cra MCAODC_01200 0.78 +0.9 224,388 + cra MCAODC_01200 0.81 -0.1 224,388 + cra MCAODC_01200 0.81 +2.0 224,388 + cra MCAODC_01200 0.81 -2.1 224,389 - cra MCAODC_01200 0.81 +0.1 224,438 + cra MCAODC_01200 0.86 +0.5 224,438 + cra MCAODC_01200 0.86 +1.7 224,439 - cra MCAODC_01200 0.86 +0.8 224,439 - cra MCAODC_01200 0.86 -0.4 224,495 + +3.0 224,495 + +0.9 224,517 + -1.1 224,517 + -1.0 224,518 - +0.1 224,518 - +0.1 224,522 + +0.8 224,522 + +0.2 224,522 + +0.2 224,523 - -0.0 224,523 - +1.0 224,523 - -0.2 224,523 - -0.3 224,593 - +0.5 224,593 - -1.2 224,646 + +0.6 224,646 + -0.6 224,647 - -0.4 224,647 - +0.3 224,647 - +0.3 224,680 - fruL MCAODC_01205 0.24 +1.1 224,771 + +0.4 224,844 + ilvN MCAODC_01210 0.18 +0.3 224,877 + ilvN MCAODC_01210 0.24 +0.5 224,878 - ilvN MCAODC_01210 0.25 +1.0 224,878 - ilvN MCAODC_01210 0.25 -2.4 224,888 + ilvN MCAODC_01210 0.27 +0.6 224,890 + ilvN MCAODC_01210 0.27 -3.2 224,891 - ilvN MCAODC_01210 0.27 +0.3 224,913 - ilvN MCAODC_01210 0.32 +0.6 224,939 + ilvN MCAODC_01210 0.37 +0.5 224,939 + ilvN MCAODC_01210 0.37 -0.3 224,940 - ilvN MCAODC_01210 0.37 -1.0 224,946 - ilvN MCAODC_01210 0.38 +0.2 224,946 - ilvN MCAODC_01210 0.38 -0.8 225,055 + ilvN MCAODC_01210 0.61 -1.1 225,055 + ilvN MCAODC_01210 0.61 +0.1 225,055 + ilvN MCAODC_01210 0.61 -1.1 225,055 + ilvN MCAODC_01210 0.61 +0.9 225,056 - ilvN MCAODC_01210 0.61 +0.8 225,056 - ilvN MCAODC_01210 0.61 -1.3 225,057 + ilvN MCAODC_01210 0.61 -0.1 225,057 + ilvN MCAODC_01210 0.61 +0.4 225,057 + ilvN MCAODC_01210 0.61 +0.9 225,058 - ilvN MCAODC_01210 0.61 -1.0 225,058 - ilvN MCAODC_01210 0.61 +0.9 225,058 - ilvN MCAODC_01210 0.61 +0.4 225,058 - ilvN MCAODC_01210 0.61 -0.5 225,058 - ilvN MCAODC_01210 0.61 -0.8 225,113 + ilvN MCAODC_01210 0.72 +0.5 225,113 + ilvN MCAODC_01210 0.72 +0.3 225,114 - ilvN MCAODC_01210 0.73 +0.3 225,167 - ilvN MCAODC_01210 0.83 +0.6 225,167 - ilvN MCAODC_01210 0.83 -0.7 225,235 - +0.1 225,367 + -0.1 225,368 - -0.5 225,392 - +0.9 225,444 + ilvI MCAODC_01215 0.11 +0.9 225,445 - ilvI MCAODC_01215 0.11 -0.8 225,445 - ilvI MCAODC_01215 0.11 +1.1 225,474 - ilvI MCAODC_01215 0.13 +0.9 225,484 + ilvI MCAODC_01215 0.14 +0.4 225,484 + ilvI MCAODC_01215 0.14 -2.3 225,485 - ilvI MCAODC_01215 0.14 +1.4 225,485 - ilvI MCAODC_01215 0.14 -0.2 225,485 - ilvI MCAODC_01215 0.14 -1.3 225,486 + ilvI MCAODC_01215 0.14 +0.7 225,486 + ilvI MCAODC_01215 0.14 +0.4 225,486 + ilvI MCAODC_01215 0.14 -0.7 225,486 + ilvI MCAODC_01215 0.14 +0.2 225,486 + ilvI MCAODC_01215 0.14 +1.0 225,487 - ilvI MCAODC_01215 0.14 -0.0 225,487 - ilvI MCAODC_01215 0.14 +0.3 225,487 - ilvI MCAODC_01215 0.14 -0.4 225,487 - ilvI MCAODC_01215 0.14 -0.1 225,487 - ilvI MCAODC_01215 0.14 -1.0 225,487 - ilvI MCAODC_01215 0.14 -1.1 225,487 - ilvI MCAODC_01215 0.14 +0.1 225,487 - ilvI MCAODC_01215 0.14 -2.6 225,510 + ilvI MCAODC_01215 0.15 -0.1 225,511 - ilvI MCAODC_01215 0.15 +0.3 225,511 - ilvI MCAODC_01215 0.15 -0.8 225,547 - ilvI MCAODC_01215 0.17 -0.3 225,556 + ilvI MCAODC_01215 0.18 +0.0 225,556 + ilvI MCAODC_01215 0.18 -0.2 225,556 + ilvI MCAODC_01215 0.18 +0.6 225,557 - ilvI MCAODC_01215 0.18 -0.1 225,586 - ilvI MCAODC_01215 0.19 -0.1 225,586 - ilvI MCAODC_01215 0.19 +0.8 225,709 + ilvI MCAODC_01215 0.27 +0.0 225,710 - ilvI MCAODC_01215 0.27 -1.0 225,759 + ilvI MCAODC_01215 0.29 -2.7 225,762 + ilvI MCAODC_01215 0.30 -1.8 225,762 + ilvI MCAODC_01215 0.30 -0.2 225,763 - ilvI MCAODC_01215 0.30 +0.4 225,802 + ilvI MCAODC_01215 0.32 +0.5 225,803 - ilvI MCAODC_01215 0.32 +0.4 225,807 + ilvI MCAODC_01215 0.32 -0.6 225,882 + ilvI MCAODC_01215 0.37 +0.0 225,883 - ilvI MCAODC_01215 0.37 +2.0 226,054 + ilvI MCAODC_01215 0.47 +0.5 226,054 + ilvI MCAODC_01215 0.47 -0.3 226,054 + ilvI MCAODC_01215 0.47 +0.3 226,055 - ilvI MCAODC_01215 0.47 +1.6 226,055 - ilvI MCAODC_01215 0.47 +0.4 226,095 + ilvI MCAODC_01215 0.49 -0.4 226,096 - ilvI MCAODC_01215 0.49 +0.1 226,163 - ilvI MCAODC_01215 0.53 +0.5 226,175 - ilvI MCAODC_01215 0.54 -0.3 226,186 - ilvI MCAODC_01215 0.54 -0.9 226,186 - ilvI MCAODC_01215 0.54 +0.3 226,186 - ilvI MCAODC_01215 0.54 -1.8 226,190 - ilvI MCAODC_01215 0.54 +0.3 226,337 + ilvI MCAODC_01215 0.63 +0.2 226,338 - ilvI MCAODC_01215 0.63 +0.8 226,338 - ilvI MCAODC_01215 0.63 -0.0 226,341 + ilvI MCAODC_01215 0.63 -1.6 226,341 + ilvI MCAODC_01215 0.63 +0.1 226,342 - ilvI MCAODC_01215 0.63 +0.4 226,399 + ilvI MCAODC_01215 0.67 -0.6 226,400 - ilvI MCAODC_01215 0.67 +0.1 226,400 - ilvI MCAODC_01215 0.67 +0.0 226,400 - ilvI MCAODC_01215 0.67 +0.0 226,411 + ilvI MCAODC_01215 0.67 -0.9 226,411 + ilvI MCAODC_01215 0.67 -0.5 226,412 - ilvI MCAODC_01215 0.67 +0.3 226,412 - ilvI MCAODC_01215 0.67 +0.2 226,422 + ilvI MCAODC_01215 0.68 +0.3 226,422 + ilvI MCAODC_01215 0.68 -0.4 226,423 - ilvI MCAODC_01215 0.68 +0.1 226,455 + ilvI MCAODC_01215 0.70 -1.0 226,455 + ilvI MCAODC_01215 0.70 +1.2 226,455 + ilvI MCAODC_01215 0.70 +1.1 226,456 - ilvI MCAODC_01215 0.70 -0.2 226,477 + ilvI MCAODC_01215 0.71 -0.1 226,483 + ilvI MCAODC_01215 0.71 -0.1 226,483 + ilvI MCAODC_01215 0.71 +0.0 226,483 + ilvI MCAODC_01215 0.71 -0.0 226,647 + ilvI MCAODC_01215 0.81 +0.2 226,648 - ilvI MCAODC_01215 0.81 -1.2 226,653 - ilvI MCAODC_01215 0.81 +0.5 226,688 + ilvI MCAODC_01215 0.83 +0.1 226,688 + ilvI MCAODC_01215 0.83 +0.8 226,688 + ilvI MCAODC_01215 0.83 -1.3 226,688 + ilvI MCAODC_01215 0.83 -0.6 226,689 - ilvI MCAODC_01215 0.83 +1.0 226,705 + ilvI MCAODC_01215 0.84 +1.5 226,705 + ilvI MCAODC_01215 0.84 -0.0 226,706 - ilvI MCAODC_01215 0.84 +0.3 226,706 - ilvI MCAODC_01215 0.84 -0.1 226,706 - ilvI MCAODC_01215 0.84 -0.7 226,706 - ilvI MCAODC_01215 0.84 -1.4 226,707 + ilvI MCAODC_01215 0.84 +0.0 226,707 + ilvI MCAODC_01215 0.84 -0.0 226,708 - ilvI MCAODC_01215 0.84 +0.2 226,708 - ilvI MCAODC_01215 0.84 -0.1 226,757 + ilvI MCAODC_01215 0.87 +1.0 226,807 + -0.3 226,807 + +0.6 226,808 - -1.1 226,836 - -1.3 226,875 + -0.1 226,875 + +0.5 226,876 - +0.3 226,876 - +0.5 226,876 - -0.3 226,876 - -0.1 226,910 + +0.4 226,911 - -0.2 226,919 - +0.4 227,019 - +0.2 227,060 - +0.7 227,060 - +0.5 227,073 + -1.0 227,073 + -0.7 227,121 + +0.2 227,139 + -0.9 227,165 + -1.6 227,202 + -1.4 227,279 + +0.1 227,314 + +0.2 227,344 - -0.8 227,375 + +0.6 227,375 + +0.5 227,376 - -1.7 227,376 - -0.0 227,389 - leuO MCAODC_01220 0.10 +2.6 227,414 - leuO MCAODC_01220 0.13 +0.7 227,419 + leuO MCAODC_01220 0.13 -0.2 227,419 + leuO MCAODC_01220 0.13 +1.0 227,419 + leuO MCAODC_01220 0.13 -0.7 227,496 + leuO MCAODC_01220 0.21 +0.1 227,497 - leuO MCAODC_01220 0.22 -0.4 227,497 - leuO MCAODC_01220 0.22 -0.1 227,500 + leuO MCAODC_01220 0.22 -0.6 227,500 + leuO MCAODC_01220 0.22 +0.9 227,501 - leuO MCAODC_01220 0.22 +1.1 227,501 - leuO MCAODC_01220 0.22 -0.2 227,501 - leuO MCAODC_01220 0.22 -0.7 227,544 + leuO MCAODC_01220 0.27 -0.6 227,545 - leuO MCAODC_01220 0.27 +0.6 227,545 - leuO MCAODC_01220 0.27 +1.7 227,545 - leuO MCAODC_01220 0.27 -0.4 227,545 - leuO MCAODC_01220 0.27 +0.1 227,548 + leuO MCAODC_01220 0.27 +0.8 227,548 + leuO MCAODC_01220 0.27 -2.1 227,571 + leuO MCAODC_01220 0.29 -0.2 227,616 + leuO MCAODC_01220 0.34 +1.1 227,671 + leuO MCAODC_01220 0.40 +0.9 227,696 - leuO MCAODC_01220 0.43 -0.2 227,736 - leuO MCAODC_01220 0.47 -1.1 227,739 + leuO MCAODC_01220 0.47 +1.8 227,740 - leuO MCAODC_01220 0.47 +0.3 227,740 - leuO MCAODC_01220 0.47 +0.2 227,791 + leuO MCAODC_01220 0.53 -2.1 227,808 + leuO MCAODC_01220 0.54 +0.5 227,808 + leuO MCAODC_01220 0.54 +0.0 227,809 - leuO MCAODC_01220 0.55 +2.0 227,809 - leuO MCAODC_01220 0.55 -3.3 227,809 - leuO MCAODC_01220 0.55 +0.3 227,815 + leuO MCAODC_01220 0.55 +0.2 227,815 + leuO MCAODC_01220 0.55 -0.7 227,815 + leuO MCAODC_01220 0.55 +1.5 227,816 - leuO MCAODC_01220 0.55 -0.4 227,817 + leuO MCAODC_01220 0.55 +0.1 227,818 - leuO MCAODC_01220 0.56 -0.1 227,818 - leuO MCAODC_01220 0.56 -0.1 227,844 + leuO MCAODC_01220 0.58 +0.2 227,899 + leuO MCAODC_01220 0.64 +0.1 227,899 + leuO MCAODC_01220 0.64 +0.1 227,953 + leuO MCAODC_01220 0.70 -1.2
Or see this region's nucleotide sequence