Experiment: WM_RB68
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speE and speD are separated by 15 nucleotides speD and yacL are separated by 37 nucleotides yacL and acnB are separated by 175 nucleotides
MCAODC_00980: speE - polyamine aminopropyltransferase, at 176,374 to 177,240
speE
MCAODC_00985: speD - adenosylmethionine decarboxylase, at 177,256 to 178,050
speD
MCAODC_00990: yacL - protein YacL, at 178,088 to 178,450
yacL
MCAODC_00995: acnB - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 178,626 to 181,223
acnB
Position (kb) 178
179 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 177.088 kb on - strand, within speE at 177.161 kb on - strand at 177.163 kb on + strand at 177.163 kb on + strand at 177.164 kb on - strand at 177.164 kb on - strand at 177.198 kb on - strand at 177.205 kb on + strand at 177.239 kb on - strand at 177.239 kb on - strand at 177.285 kb on + strand at 177.286 kb on - strand at 177.286 kb on - strand at 177.316 kb on + strand at 177.317 kb on - strand at 177.322 kb on - strand at 177.322 kb on - strand at 177.329 kb on - strand at 177.358 kb on + strand, within speD at 177.358 kb on + strand, within speD at 177.358 kb on + strand, within speD at 177.358 kb on + strand, within speD at 177.359 kb on - strand, within speD at 177.359 kb on - strand, within speD at 177.360 kb on + strand, within speD at 177.360 kb on + strand, within speD at 177.360 kb on + strand, within speD at 177.360 kb on + strand, within speD at 177.361 kb on - strand, within speD at 177.361 kb on - strand, within speD at 177.361 kb on - strand, within speD at 177.367 kb on + strand, within speD at 177.367 kb on + strand, within speD at 177.368 kb on - strand, within speD at 177.368 kb on - strand, within speD at 177.369 kb on + strand, within speD at 177.369 kb on + strand, within speD at 177.369 kb on + strand, within speD at 177.382 kb on + strand, within speD at 177.383 kb on - strand, within speD at 177.383 kb on - strand, within speD at 177.383 kb on - strand, within speD at 177.384 kb on + strand, within speD at 177.385 kb on - strand, within speD at 177.438 kb on + strand, within speD at 177.438 kb on + strand, within speD at 177.447 kb on + strand, within speD at 177.595 kb on - strand, within speD at 177.595 kb on - strand, within speD at 177.595 kb on - strand, within speD at 177.602 kb on + strand, within speD at 177.602 kb on + strand, within speD at 177.602 kb on + strand, within speD at 177.602 kb on + strand, within speD at 177.603 kb on - strand, within speD at 177.606 kb on + strand, within speD at 177.606 kb on + strand, within speD at 177.606 kb on + strand, within speD at 177.606 kb on + strand, within speD at 177.606 kb on + strand, within speD at 177.606 kb on + strand, within speD at 177.606 kb on + strand, within speD at 177.606 kb on + strand, within speD at 177.606 kb on + strand, within speD at 177.606 kb on + strand, within speD at 177.606 kb on + strand, within speD at 177.606 kb on + strand, within speD at 177.607 kb on - strand, within speD at 177.607 kb on - strand, within speD at 177.607 kb on - strand, within speD at 177.607 kb on - strand, within speD at 177.607 kb on - strand, within speD at 177.607 kb on - strand, within speD at 177.607 kb on - strand, within speD at 177.607 kb on - strand, within speD at 177.607 kb on - strand, within speD at 177.611 kb on - strand, within speD at 177.638 kb on + strand, within speD at 177.638 kb on + strand, within speD at 177.638 kb on + strand, within speD at 177.639 kb on - strand, within speD at 177.639 kb on - strand, within speD at 177.639 kb on - strand, within speD at 177.707 kb on - strand, within speD at 177.707 kb on - strand, within speD at 177.707 kb on - strand, within speD at 177.737 kb on + strand, within speD at 177.777 kb on + strand, within speD at 177.784 kb on - strand, within speD at 177.784 kb on - strand, within speD at 177.810 kb on + strand, within speD at 177.810 kb on + strand, within speD at 177.829 kb on - strand, within speD at 177.829 kb on - strand, within speD at 177.854 kb on - strand, within speD at 177.854 kb on - strand, within speD at 177.854 kb on - strand, within speD at 177.869 kb on + strand, within speD at 177.878 kb on - strand, within speD at 177.934 kb on + strand, within speD at 177.935 kb on - strand, within speD at 177.935 kb on - strand, within speD at 177.935 kb on - strand, within speD at 177.935 kb on - strand, within speD at 177.963 kb on - strand, within speD at 177.963 kb on - strand, within speD at 178.022 kb on - strand at 178.022 kb on - strand at 178.085 kb on + strand at 178.085 kb on + strand at 178.089 kb on + strand at 178.090 kb on - strand at 178.090 kb on - strand at 178.151 kb on + strand, within yacL at 178.173 kb on + strand, within yacL at 178.174 kb on - strand, within yacL at 178.174 kb on - strand, within yacL at 178.176 kb on + strand, within yacL at 178.177 kb on - strand, within yacL at 178.261 kb on + strand, within yacL at 178.261 kb on + strand, within yacL at 178.261 kb on + strand, within yacL at 178.261 kb on + strand, within yacL at 178.261 kb on + strand, within yacL at 178.261 kb on + strand, within yacL at 178.262 kb on - strand, within yacL at 178.262 kb on - strand, within yacL at 178.262 kb on - strand, within yacL at 178.262 kb on - strand, within yacL at 178.263 kb on + strand, within yacL at 178.263 kb on + strand, within yacL at 178.263 kb on + strand, within yacL at 178.263 kb on + strand, within yacL at 178.264 kb on - strand, within yacL at 178.367 kb on - strand, within yacL at 178.367 kb on - strand, within yacL at 178.367 kb on - strand, within yacL at 178.402 kb on + strand, within yacL at 178.402 kb on + strand, within yacL at 178.402 kb on + strand, within yacL at 178.402 kb on + strand, within yacL at 178.402 kb on + strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.450 kb on + strand at 178.504 kb on + strand at 178.504 kb on + strand at 178.504 kb on + strand at 178.504 kb on + strand at 178.504 kb on + strand at 178.505 kb on - strand at 178.505 kb on - strand at 178.505 kb on - strand at 178.505 kb on - strand at 178.505 kb on - strand at 178.505 kb on - strand at 178.509 kb on - strand at 178.551 kb on + strand at 178.703 kb on - strand at 178.703 kb on - strand at 178.703 kb on - strand at 178.707 kb on - strand at 179.192 kb on + strand, within acnB
Per-strain Table
Position Strand Gene LocusTag Fraction WM_RB68 remove 177,088 - speE MCAODC_00980 0.82 -0.2 177,161 - -0.5 177,163 + -0.3 177,163 + +0.3 177,164 - -0.6 177,164 - -1.2 177,198 - +0.6 177,205 + +0.1 177,239 - -3.4 177,239 - +1.3 177,285 + -2.0 177,286 - -0.2 177,286 - +0.2 177,316 + +0.1 177,317 - +0.0 177,322 - -0.0 177,322 - -1.0 177,329 - -1.9 177,358 + speD MCAODC_00985 0.13 +1.4 177,358 + speD MCAODC_00985 0.13 +0.2 177,358 + speD MCAODC_00985 0.13 +0.4 177,358 + speD MCAODC_00985 0.13 +0.9 177,359 - speD MCAODC_00985 0.13 -0.2 177,359 - speD MCAODC_00985 0.13 +0.1 177,360 + speD MCAODC_00985 0.13 +0.4 177,360 + speD MCAODC_00985 0.13 +0.1 177,360 + speD MCAODC_00985 0.13 -0.4 177,360 + speD MCAODC_00985 0.13 +1.1 177,361 - speD MCAODC_00985 0.13 +0.2 177,361 - speD MCAODC_00985 0.13 +0.5 177,361 - speD MCAODC_00985 0.13 -0.2 177,367 + speD MCAODC_00985 0.14 +1.5 177,367 + speD MCAODC_00985 0.14 +0.4 177,368 - speD MCAODC_00985 0.14 +0.3 177,368 - speD MCAODC_00985 0.14 -0.5 177,369 + speD MCAODC_00985 0.14 +0.6 177,369 + speD MCAODC_00985 0.14 -0.7 177,369 + speD MCAODC_00985 0.14 +2.3 177,382 + speD MCAODC_00985 0.16 +0.0 177,383 - speD MCAODC_00985 0.16 -0.4 177,383 - speD MCAODC_00985 0.16 +0.8 177,383 - speD MCAODC_00985 0.16 -0.4 177,384 + speD MCAODC_00985 0.16 -0.4 177,385 - speD MCAODC_00985 0.16 -0.2 177,438 + speD MCAODC_00985 0.23 +0.0 177,438 + speD MCAODC_00985 0.23 +1.5 177,447 + speD MCAODC_00985 0.24 -0.8 177,595 - speD MCAODC_00985 0.43 +0.3 177,595 - speD MCAODC_00985 0.43 +1.2 177,595 - speD MCAODC_00985 0.43 +1.1 177,602 + speD MCAODC_00985 0.44 -0.1 177,602 + speD MCAODC_00985 0.44 -0.3 177,602 + speD MCAODC_00985 0.44 +0.7 177,602 + speD MCAODC_00985 0.44 +0.0 177,603 - speD MCAODC_00985 0.44 -0.9 177,606 + speD MCAODC_00985 0.44 -0.1 177,606 + speD MCAODC_00985 0.44 -2.6 177,606 + speD MCAODC_00985 0.44 +0.6 177,606 + speD MCAODC_00985 0.44 -0.6 177,606 + speD MCAODC_00985 0.44 -0.0 177,606 + speD MCAODC_00985 0.44 -0.8 177,606 + speD MCAODC_00985 0.44 +0.6 177,606 + speD MCAODC_00985 0.44 +0.3 177,606 + speD MCAODC_00985 0.44 -1.1 177,606 + speD MCAODC_00985 0.44 -1.7 177,606 + speD MCAODC_00985 0.44 +1.1 177,606 + speD MCAODC_00985 0.44 +0.8 177,607 - speD MCAODC_00985 0.44 -0.7 177,607 - speD MCAODC_00985 0.44 +1.1 177,607 - speD MCAODC_00985 0.44 +0.7 177,607 - speD MCAODC_00985 0.44 +0.4 177,607 - speD MCAODC_00985 0.44 +0.1 177,607 - speD MCAODC_00985 0.44 -0.9 177,607 - speD MCAODC_00985 0.44 +0.6 177,607 - speD MCAODC_00985 0.44 +0.0 177,607 - speD MCAODC_00985 0.44 +0.8 177,611 - speD MCAODC_00985 0.45 -0.1 177,638 + speD MCAODC_00985 0.48 +0.4 177,638 + speD MCAODC_00985 0.48 +0.7 177,638 + speD MCAODC_00985 0.48 +0.9 177,639 - speD MCAODC_00985 0.48 +0.3 177,639 - speD MCAODC_00985 0.48 +0.0 177,639 - speD MCAODC_00985 0.48 -0.1 177,707 - speD MCAODC_00985 0.57 -3.1 177,707 - speD MCAODC_00985 0.57 +0.2 177,707 - speD MCAODC_00985 0.57 -0.2 177,737 + speD MCAODC_00985 0.61 -0.2 177,777 + speD MCAODC_00985 0.66 +0.4 177,784 - speD MCAODC_00985 0.66 -0.7 177,784 - speD MCAODC_00985 0.66 +1.4 177,810 + speD MCAODC_00985 0.70 +0.3 177,810 + speD MCAODC_00985 0.70 -0.3 177,829 - speD MCAODC_00985 0.72 -2.2 177,829 - speD MCAODC_00985 0.72 -1.8 177,854 - speD MCAODC_00985 0.75 +0.1 177,854 - speD MCAODC_00985 0.75 -0.5 177,854 - speD MCAODC_00985 0.75 +0.5 177,869 + speD MCAODC_00985 0.77 +0.2 177,878 - speD MCAODC_00985 0.78 +0.2 177,934 + speD MCAODC_00985 0.85 -2.2 177,935 - speD MCAODC_00985 0.85 -0.2 177,935 - speD MCAODC_00985 0.85 -0.8 177,935 - speD MCAODC_00985 0.85 +0.0 177,935 - speD MCAODC_00985 0.85 -0.0 177,963 - speD MCAODC_00985 0.89 -1.1 177,963 - speD MCAODC_00985 0.89 +1.1 178,022 - +0.1 178,022 - +0.5 178,085 + -0.2 178,085 + +0.2 178,089 + -0.5 178,090 - +0.5 178,090 - +0.7 178,151 + yacL MCAODC_00990 0.17 +0.1 178,173 + yacL MCAODC_00990 0.23 -0.7 178,174 - yacL MCAODC_00990 0.24 +0.2 178,174 - yacL MCAODC_00990 0.24 -0.7 178,176 + yacL MCAODC_00990 0.24 -1.9 178,177 - yacL MCAODC_00990 0.25 +0.4 178,261 + yacL MCAODC_00990 0.48 +0.7 178,261 + yacL MCAODC_00990 0.48 -1.3 178,261 + yacL MCAODC_00990 0.48 -1.0 178,261 + yacL MCAODC_00990 0.48 +0.3 178,261 + yacL MCAODC_00990 0.48 +0.3 178,261 + yacL MCAODC_00990 0.48 +0.7 178,262 - yacL MCAODC_00990 0.48 -2.0 178,262 - yacL MCAODC_00990 0.48 +0.5 178,262 - yacL MCAODC_00990 0.48 -0.1 178,262 - yacL MCAODC_00990 0.48 +2.0 178,263 + yacL MCAODC_00990 0.48 +0.5 178,263 + yacL MCAODC_00990 0.48 -0.6 178,263 + yacL MCAODC_00990 0.48 -1.7 178,263 + yacL MCAODC_00990 0.48 -1.1 178,264 - yacL MCAODC_00990 0.48 -0.6 178,367 - yacL MCAODC_00990 0.77 +0.7 178,367 - yacL MCAODC_00990 0.77 +0.4 178,367 - yacL MCAODC_00990 0.77 -0.9 178,402 + yacL MCAODC_00990 0.87 -0.6 178,402 + yacL MCAODC_00990 0.87 -1.0 178,402 + yacL MCAODC_00990 0.87 +0.4 178,402 + yacL MCAODC_00990 0.87 -0.9 178,402 + yacL MCAODC_00990 0.87 -0.3 178,403 - yacL MCAODC_00990 0.87 +0.5 178,403 - yacL MCAODC_00990 0.87 -0.4 178,403 - yacL MCAODC_00990 0.87 +1.1 178,403 - yacL MCAODC_00990 0.87 +0.5 178,403 - yacL MCAODC_00990 0.87 -1.6 178,403 - yacL MCAODC_00990 0.87 -0.7 178,403 - yacL MCAODC_00990 0.87 -1.4 178,450 + +1.0 178,504 + -0.6 178,504 + -0.0 178,504 + -0.1 178,504 + +0.9 178,504 + -0.1 178,505 - +1.0 178,505 - -0.4 178,505 - +0.2 178,505 - +0.5 178,505 - +0.6 178,505 - -0.4 178,509 - +0.9 178,551 + -0.2 178,703 - -2.2 178,703 - +0.3 178,703 - -1.0 178,707 - +0.3 179,192 + acnB MCAODC_00995 0.22 -0.6
Or see this region's nucleotide sequence