Experiment: WM_TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_20595 and wcaF are separated by 28 nucleotides wcaF and wcaE are separated by 15 nucleotides wcaE and wcaD are separated by 10 nucleotides
MCAODC_20595: MCAODC_20595 - GDP-mannose 4,6-dehydratase, at 930,455 to 931,576
_20595
MCAODC_20600: wcaF - colanic acid biosynthesis acetyltransferase WcaF, at 931,605 to 932,153
wcaF
MCAODC_20605: wcaE - colanic acid biosynthesis glycosyltransferase WcaE, at 932,169 to 932,915
wcaE
MCAODC_20610: wcaD - putative colanic acid polymerase WcaD, at 932,926 to 934,143
wcaD
Position (kb) 932
933 Strain fitness (log2 ratio) -2
-1
0
1
2 at 931.191 kb on + strand, within MCAODC_20595 at 931.273 kb on + strand, within MCAODC_20595 at 931.273 kb on + strand, within MCAODC_20595 at 931.326 kb on + strand, within MCAODC_20595 at 931.327 kb on - strand, within MCAODC_20595 at 931.327 kb on - strand, within MCAODC_20595 at 931.327 kb on - strand, within MCAODC_20595 at 931.358 kb on - strand, within MCAODC_20595 at 931.393 kb on - strand, within MCAODC_20595 at 931.435 kb on - strand, within MCAODC_20595 at 931.480 kb on + strand at 931.481 kb on - strand at 931.680 kb on - strand, within wcaF at 931.782 kb on - strand, within wcaF at 931.782 kb on - strand, within wcaF at 931.782 kb on - strand, within wcaF at 931.837 kb on - strand, within wcaF at 931.896 kb on + strand, within wcaF at 931.897 kb on - strand, within wcaF at 931.933 kb on - strand, within wcaF at 932.041 kb on - strand, within wcaF at 932.097 kb on - strand, within wcaF at 932.100 kb on - strand at 932.207 kb on + strand at 932.208 kb on - strand at 932.615 kb on - strand, within wcaE at 932.705 kb on - strand, within wcaE at 932.737 kb on - strand, within wcaE at 932.744 kb on - strand, within wcaE at 932.922 kb on - strand at 932.959 kb on - strand at 933.535 kb on - strand, within wcaD at 933.671 kb on + strand, within wcaD at 933.671 kb on + strand, within wcaD at 933.799 kb on + strand, within wcaD at 933.855 kb on + strand, within wcaD at 933.873 kb on + strand, within wcaD at 933.874 kb on - strand, within wcaD at 933.874 kb on - strand, within wcaD at 933.875 kb on + strand, within wcaD at 933.888 kb on + strand, within wcaD at 933.888 kb on + strand, within wcaD at 933.888 kb on + strand, within wcaD at 933.902 kb on + strand, within wcaD at 933.902 kb on + strand, within wcaD
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP9 remove 931,191 + MCAODC_20595 0.66 +0.0 931,273 + MCAODC_20595 0.73 +1.4 931,273 + MCAODC_20595 0.73 +0.5 931,326 + MCAODC_20595 0.78 +1.2 931,327 - MCAODC_20595 0.78 +0.4 931,327 - MCAODC_20595 0.78 +1.0 931,327 - MCAODC_20595 0.78 -0.1 931,358 - MCAODC_20595 0.80 -1.6 931,393 - MCAODC_20595 0.84 -0.3 931,435 - MCAODC_20595 0.87 -0.3 931,480 + +0.7 931,481 - -1.5 931,680 - wcaF MCAODC_20600 0.14 -1.5 931,782 - wcaF MCAODC_20600 0.32 -1.3 931,782 - wcaF MCAODC_20600 0.32 -0.4 931,782 - wcaF MCAODC_20600 0.32 +0.4 931,837 - wcaF MCAODC_20600 0.42 +0.1 931,896 + wcaF MCAODC_20600 0.53 +0.2 931,897 - wcaF MCAODC_20600 0.53 +1.0 931,933 - wcaF MCAODC_20600 0.60 -0.3 932,041 - wcaF MCAODC_20600 0.79 -2.0 932,097 - wcaF MCAODC_20600 0.90 -0.6 932,100 - +0.0 932,207 + +0.2 932,208 - +1.1 932,615 - wcaE MCAODC_20605 0.60 +0.2 932,705 - wcaE MCAODC_20605 0.72 -0.2 932,737 - wcaE MCAODC_20605 0.76 +2.5 932,744 - wcaE MCAODC_20605 0.77 -0.5 932,922 - -0.5 932,959 - +0.1 933,535 - wcaD MCAODC_20610 0.50 +0.3 933,671 + wcaD MCAODC_20610 0.61 -0.9 933,671 + wcaD MCAODC_20610 0.61 +2.0 933,799 + wcaD MCAODC_20610 0.72 +0.3 933,855 + wcaD MCAODC_20610 0.76 -0.2 933,873 + wcaD MCAODC_20610 0.78 -1.3 933,874 - wcaD MCAODC_20610 0.78 -1.1 933,874 - wcaD MCAODC_20610 0.78 +0.7 933,875 + wcaD MCAODC_20610 0.78 +0.0 933,888 + wcaD MCAODC_20610 0.79 +0.5 933,888 + wcaD MCAODC_20610 0.79 -0.1 933,888 + wcaD MCAODC_20610 0.79 +0.9 933,902 + wcaD MCAODC_20610 0.80 -1.0 933,902 + wcaD MCAODC_20610 0.80 -0.1
Or see this region's nucleotide sequence