Strain Fitness in Escherichia coli ECRC101 around MCAODC_18260

Experiment: WM_TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydiS and ydiT overlap by 4 nucleotidesydiT and fadK are separated by 56 nucleotidesfadK and ppsA are separated by 160 nucleotides MCAODC_18250: ydiS - putative electron transfer flavoprotein-quinone oxidoreductase YdiS, at 510,378 to 511,667 ydiS MCAODC_18255: ydiT - Ferredoxin-like protein YdiT, at 511,664 to 511,957 ydiT MCAODC_18260: fadK - medium-chain fatty-acid--CoA ligase, at 512,014 to 513,555 fadK MCAODC_18265: ppsA - phosphoenolpyruvate synthase, at 513,716 to 516,094 ppsA Position (kb) 512 513 514Strain fitness (log2 ratio) -1 0 1 2at 511.058 kb on + strand, within ydiSat 511.214 kb on + strand, within ydiSat 511.313 kb on + strand, within ydiSat 511.420 kb on - strand, within ydiSat 511.781 kb on - strand, within ydiTat 511.813 kb on + strand, within ydiTat 511.813 kb on + strand, within ydiTat 511.917 kb on - strand, within ydiTat 511.956 kb on - strandat 512.164 kb on + strandat 512.165 kb on - strandat 512.165 kb on - strandat 512.170 kb on + strand, within fadKat 512.170 kb on + strand, within fadKat 512.171 kb on - strand, within fadKat 512.171 kb on - strand, within fadKat 512.171 kb on - strand, within fadKat 512.173 kb on - strand, within fadKat 512.283 kb on + strand, within fadKat 512.284 kb on - strand, within fadKat 512.287 kb on + strand, within fadKat 512.287 kb on + strand, within fadKat 512.287 kb on + strand, within fadKat 512.322 kb on - strand, within fadKat 512.367 kb on + strand, within fadKat 512.371 kb on - strand, within fadKat 512.395 kb on - strand, within fadKat 512.432 kb on + strand, within fadKat 512.465 kb on + strand, within fadKat 512.466 kb on - strand, within fadKat 512.519 kb on + strand, within fadKat 512.643 kb on + strand, within fadKat 512.649 kb on + strand, within fadKat 512.650 kb on - strand, within fadKat 512.672 kb on - strand, within fadKat 512.710 kb on - strand, within fadKat 512.772 kb on - strand, within fadKat 512.895 kb on - strand, within fadKat 513.002 kb on + strand, within fadKat 513.003 kb on - strand, within fadKat 513.003 kb on - strand, within fadKat 513.013 kb on + strand, within fadKat 513.076 kb on - strand, within fadKat 513.108 kb on - strand, within fadKat 513.218 kb on + strand, within fadKat 513.294 kb on - strand, within fadKat 513.321 kb on + strand, within fadKat 513.448 kb on + strandat 513.781 kb on + strandat 513.856 kb on - strandat 514.015 kb on - strand, within ppsAat 514.142 kb on + strand, within ppsAat 514.142 kb on + strand, within ppsAat 514.257 kb on + strand, within ppsAat 514.541 kb on - strand, within ppsA

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP9
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511,058 + ydiS MCAODC_18250 0.53 +1.0
511,214 + ydiS MCAODC_18250 0.65 -0.2
511,313 + ydiS MCAODC_18250 0.72 +1.0
511,420 - ydiS MCAODC_18250 0.81 -0.3
511,781 - ydiT MCAODC_18255 0.40 -0.3
511,813 + ydiT MCAODC_18255 0.51 -0.4
511,813 + ydiT MCAODC_18255 0.51 -0.1
511,917 - ydiT MCAODC_18255 0.86 -0.3
511,956 - +0.6
512,164 + -0.0
512,165 - -0.0
512,165 - +0.6
512,170 + fadK MCAODC_18260 0.10 -0.0
512,170 + fadK MCAODC_18260 0.10 +0.9
512,171 - fadK MCAODC_18260 0.10 -0.3
512,171 - fadK MCAODC_18260 0.10 -1.4
512,171 - fadK MCAODC_18260 0.10 -0.4
512,173 - fadK MCAODC_18260 0.10 +0.4
512,283 + fadK MCAODC_18260 0.17 -0.2
512,284 - fadK MCAODC_18260 0.18 +1.6
512,287 + fadK MCAODC_18260 0.18 +1.0
512,287 + fadK MCAODC_18260 0.18 +1.0
512,287 + fadK MCAODC_18260 0.18 -0.1
512,322 - fadK MCAODC_18260 0.20 +2.2
512,367 + fadK MCAODC_18260 0.23 +1.9
512,371 - fadK MCAODC_18260 0.23 +1.0
512,395 - fadK MCAODC_18260 0.25 +0.6
512,432 + fadK MCAODC_18260 0.27 +0.4
512,465 + fadK MCAODC_18260 0.29 +1.9
512,466 - fadK MCAODC_18260 0.29 +1.4
512,519 + fadK MCAODC_18260 0.33 -1.1
512,643 + fadK MCAODC_18260 0.41 +0.8
512,649 + fadK MCAODC_18260 0.41 +1.0
512,650 - fadK MCAODC_18260 0.41 +0.9
512,672 - fadK MCAODC_18260 0.43 +0.2
512,710 - fadK MCAODC_18260 0.45 +0.1
512,772 - fadK MCAODC_18260 0.49 +0.4
512,895 - fadK MCAODC_18260 0.57 +0.8
513,002 + fadK MCAODC_18260 0.64 +0.4
513,003 - fadK MCAODC_18260 0.64 -0.3
513,003 - fadK MCAODC_18260 0.64 +0.1
513,013 + fadK MCAODC_18260 0.65 -0.6
513,076 - fadK MCAODC_18260 0.69 +0.3
513,108 - fadK MCAODC_18260 0.71 -0.8
513,218 + fadK MCAODC_18260 0.78 -0.9
513,294 - fadK MCAODC_18260 0.83 +0.0
513,321 + fadK MCAODC_18260 0.85 +0.0
513,448 + -0.1
513,781 + +1.2
513,856 - -0.8
514,015 - ppsA MCAODC_18265 0.13 +0.1
514,142 + ppsA MCAODC_18265 0.18 +0.1
514,142 + ppsA MCAODC_18265 0.18 +0.4
514,257 + ppsA MCAODC_18265 0.23 +1.0
514,541 - ppsA MCAODC_18265 0.35 -0.3

Or see this region's nucleotide sequence