Experiment: WM_TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt clcB and bioD overlap by 48 nucleotides bioD and mlc are separated by 124 nucleotides mlc and ynfL are separated by 134 nucleotides
MCAODC_17660: clcB - voltage-gated ClC-type chloride channel ClcB, at 394,385 to 395,641
clcB
MCAODC_17665: bioD - dethiobiotin synthase, at 395,594 to 396,289
bioD
MCAODC_17670: mlc - DNA-binding transcriptional repressor Mlc, at 396,414 to 397,634
mlc
MCAODC_17675: ynfL - Uncharacterized HTH-type transcriptional regulator YnfL, at 397,769 to 398,662
ynfL
Position (kb) 396
397
398 Strain fitness (log2 ratio) -1
0
1
2
3 at 395.498 kb on - strand, within clcB at 395.514 kb on + strand, within clcB at 395.541 kb on + strand at 395.541 kb on + strand at 395.569 kb on - strand at 395.636 kb on - strand at 395.640 kb on - strand at 395.640 kb on - strand at 395.650 kb on - strand at 395.650 kb on - strand at 396.178 kb on + strand, within bioD at 396.318 kb on + strand at 396.321 kb on - strand at 396.337 kb on + strand at 396.351 kb on + strand at 396.356 kb on + strand at 396.357 kb on - strand at 396.357 kb on - strand at 396.451 kb on + strand at 396.451 kb on + strand at 396.669 kb on + strand, within mlc at 396.816 kb on - strand, within mlc at 396.878 kb on - strand, within mlc at 396.885 kb on + strand, within mlc at 396.886 kb on - strand, within mlc at 396.886 kb on - strand, within mlc at 397.144 kb on - strand, within mlc at 397.376 kb on - strand, within mlc at 397.497 kb on - strand, within mlc at 397.674 kb on - strand at 397.718 kb on + strand at 397.916 kb on - strand, within ynfL at 398.122 kb on + strand, within ynfL at 398.493 kb on - strand, within ynfL at 398.561 kb on + strand, within ynfL
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP9 remove 395,498 - clcB MCAODC_17660 0.89 +0.8 395,514 + clcB MCAODC_17660 0.90 +1.0 395,541 + +0.4 395,541 + +0.8 395,569 - +0.6 395,636 - +1.3 395,640 - -0.3 395,640 - -0.4 395,650 - -0.7 395,650 - +3.0 396,178 + bioD MCAODC_17665 0.84 -0.7 396,318 + +0.2 396,321 - +0.6 396,337 + +0.1 396,351 + -0.4 396,356 + +1.0 396,357 - -1.0 396,357 - +0.3 396,451 + +1.3 396,451 + +1.8 396,669 + mlc MCAODC_17670 0.21 +0.1 396,816 - mlc MCAODC_17670 0.33 -0.5 396,878 - mlc MCAODC_17670 0.38 +0.4 396,885 + mlc MCAODC_17670 0.39 +0.9 396,886 - mlc MCAODC_17670 0.39 +0.4 396,886 - mlc MCAODC_17670 0.39 -0.9 397,144 - mlc MCAODC_17670 0.60 -1.6 397,376 - mlc MCAODC_17670 0.79 +1.0 397,497 - mlc MCAODC_17670 0.89 -0.4 397,674 - -0.0 397,718 + -0.2 397,916 - ynfL MCAODC_17675 0.16 +0.2 398,122 + ynfL MCAODC_17675 0.39 -1.5 398,493 - ynfL MCAODC_17675 0.81 +0.5 398,561 + ynfL MCAODC_17675 0.89 +0.8
Or see this region's nucleotide sequence