Experiment: WM_TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt puuE and pspF are separated by 130 nucleotides pspF and pspA are separated by 166 nucleotides pspA and pspB are separated by 53 nucleotides
MCAODC_15850: puuE - 4-aminobutyrate transaminase, at 63,346 to 64,611
puuE
MCAODC_15855: pspF - phage shock protein operon transcriptional activator, at 64,742 to 65,719
pspF
MCAODC_15860: pspA - phage shock protein PspA, at 65,886 to 66,554
pspA
MCAODC_15865: pspB - envelope stress response membrane protein PspB, at 66,608 to 66,832
pspB
Position (kb) 64
65
66 Strain fitness (log2 ratio) -2
-1
0
1
2 at 63.765 kb on + strand, within puuE at 63.766 kb on - strand, within puuE at 63.769 kb on + strand, within puuE at 63.769 kb on + strand, within puuE at 63.771 kb on + strand, within puuE at 63.771 kb on + strand, within puuE at 63.772 kb on - strand, within puuE at 63.778 kb on - strand, within puuE at 63.842 kb on - strand, within puuE at 63.848 kb on + strand, within puuE at 63.849 kb on - strand, within puuE at 63.849 kb on - strand, within puuE at 63.915 kb on + strand, within puuE at 64.130 kb on + strand, within puuE at 64.130 kb on + strand, within puuE at 64.130 kb on + strand, within puuE at 64.194 kb on + strand, within puuE at 64.195 kb on - strand, within puuE at 64.499 kb on + strand at 64.677 kb on + strand at 64.685 kb on - strand at 64.685 kb on - strand at 64.688 kb on + strand at 64.728 kb on - strand at 64.782 kb on + strand at 64.821 kb on + strand at 64.822 kb on - strand at 64.883 kb on + strand, within pspF at 64.883 kb on + strand, within pspF at 64.967 kb on - strand, within pspF at 65.161 kb on + strand, within pspF at 65.162 kb on - strand, within pspF at 65.162 kb on - strand, within pspF at 65.344 kb on - strand, within pspF at 65.344 kb on - strand, within pspF at 65.409 kb on - strand, within pspF at 65.409 kb on - strand, within pspF at 65.539 kb on + strand, within pspF at 65.568 kb on + strand, within pspF at 65.701 kb on + strand at 65.885 kb on + strand at 65.891 kb on + strand at 65.941 kb on + strand at 65.999 kb on + strand, within pspA at 65.999 kb on + strand, within pspA at 65.999 kb on + strand, within pspA at 65.999 kb on + strand, within pspA at 66.000 kb on - strand, within pspA at 66.013 kb on + strand, within pspA at 66.065 kb on + strand, within pspA at 66.407 kb on + strand, within pspA at 66.555 kb on + strand at 66.555 kb on + strand at 66.556 kb on - strand at 66.596 kb on + strand at 66.608 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP9 remove 63,765 + puuE MCAODC_15850 0.33 -0.8 63,766 - puuE MCAODC_15850 0.33 +0.2 63,769 + puuE MCAODC_15850 0.33 +0.5 63,769 + puuE MCAODC_15850 0.33 -0.7 63,771 + puuE MCAODC_15850 0.34 -0.1 63,771 + puuE MCAODC_15850 0.34 +1.2 63,772 - puuE MCAODC_15850 0.34 +0.1 63,778 - puuE MCAODC_15850 0.34 +1.0 63,842 - puuE MCAODC_15850 0.39 -2.2 63,848 + puuE MCAODC_15850 0.40 +2.1 63,849 - puuE MCAODC_15850 0.40 +0.8 63,849 - puuE MCAODC_15850 0.40 +0.1 63,915 + puuE MCAODC_15850 0.45 +0.8 64,130 + puuE MCAODC_15850 0.62 -0.6 64,130 + puuE MCAODC_15850 0.62 +2.1 64,130 + puuE MCAODC_15850 0.62 +0.3 64,194 + puuE MCAODC_15850 0.67 -0.8 64,195 - puuE MCAODC_15850 0.67 +0.2 64,499 + -1.0 64,677 + -0.3 64,685 - -0.8 64,685 - +0.7 64,688 + -0.2 64,728 - +1.0 64,782 + -0.2 64,821 + +0.9 64,822 - +1.0 64,883 + pspF MCAODC_15855 0.14 -1.3 64,883 + pspF MCAODC_15855 0.14 +0.2 64,967 - pspF MCAODC_15855 0.23 +0.3 65,161 + pspF MCAODC_15855 0.43 +1.0 65,162 - pspF MCAODC_15855 0.43 -2.3 65,162 - pspF MCAODC_15855 0.43 +1.2 65,344 - pspF MCAODC_15855 0.62 -0.6 65,344 - pspF MCAODC_15855 0.62 +2.3 65,409 - pspF MCAODC_15855 0.68 -0.0 65,409 - pspF MCAODC_15855 0.68 +0.0 65,539 + pspF MCAODC_15855 0.81 -0.2 65,568 + pspF MCAODC_15855 0.84 +0.8 65,701 + -1.9 65,885 + +1.8 65,891 + -0.3 65,941 + -0.9 65,999 + pspA MCAODC_15860 0.17 -1.3 65,999 + pspA MCAODC_15860 0.17 -2.4 65,999 + pspA MCAODC_15860 0.17 -0.1 65,999 + pspA MCAODC_15860 0.17 +0.4 66,000 - pspA MCAODC_15860 0.17 -0.7 66,013 + pspA MCAODC_15860 0.19 +0.3 66,065 + pspA MCAODC_15860 0.27 +0.3 66,407 + pspA MCAODC_15860 0.78 -0.2 66,555 + +0.0 66,555 + -0.5 66,556 - -0.5 66,596 + +1.6 66,608 - +0.2
Or see this region's nucleotide sequence