Strain Fitness in Escherichia coli ECRC101 around MCAODC_15815

Experiment: WM_TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsapA and MCAODC_15815 are separated by 312 nucleotidesMCAODC_15815 and puuP are separated by 133 nucleotides MCAODC_15810: sapA - peptide ABC transporter substrate-binding protein SapA, at 53,388 to 55,031 sapA MCAODC_15815: MCAODC_15815 - DUF2543 domain-containing protein, at 55,344 to 55,589 _15815 MCAODC_15820: puuP - putrescine/proton symporter PuuP, at 55,723 to 57,108 puuP Position (kb) 55 56Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 55.188 kb on - strandat 55.194 kb on - strandat 55.194 kb on - strandat 55.194 kb on - strandat 55.194 kb on - strandat 55.194 kb on - strandat 55.194 kb on - strandat 55.196 kb on - strandat 55.196 kb on - strandat 55.196 kb on - strandat 55.196 kb on - strandat 55.401 kb on - strand, within MCAODC_15815at 55.486 kb on + strand, within MCAODC_15815at 55.654 kb on + strandat 55.654 kb on + strandat 55.664 kb on + strandat 55.664 kb on + strandat 55.666 kb on + strandat 55.725 kb on - strandat 55.725 kb on - strandat 55.725 kb on - strandat 55.725 kb on - strandat 55.725 kb on - strandat 55.725 kb on - strandat 55.758 kb on - strandat 55.800 kb on - strandat 55.933 kb on - strand, within puuPat 56.011 kb on - strand, within puuPat 56.032 kb on + strand, within puuPat 56.159 kb on + strand, within puuPat 56.159 kb on + strand, within puuPat 56.160 kb on - strand, within puuPat 56.261 kb on + strand, within puuPat 56.331 kb on + strand, within puuPat 56.366 kb on + strand, within puuPat 56.366 kb on + strand, within puuPat 56.367 kb on - strand, within puuPat 56.517 kb on + strand, within puuPat 56.560 kb on - strand, within puuPat 56.586 kb on + strand, within puuP

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP9
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55,188 - -0.4
55,194 - -0.3
55,194 - -0.3
55,194 - +1.1
55,194 - -0.5
55,194 - -2.9
55,194 - +0.3
55,196 - +0.5
55,196 - +0.0
55,196 - +1.0
55,196 - +0.3
55,401 - MCAODC_15815 0.23 -0.6
55,486 + MCAODC_15815 0.58 -0.3
55,654 + -1.8
55,654 + -0.6
55,664 + +0.0
55,664 + +0.6
55,666 + +1.4
55,725 - +0.4
55,725 - -0.3
55,725 - -0.3
55,725 - -0.5
55,725 - -0.6
55,725 - +0.2
55,758 - -0.4
55,800 - +2.1
55,933 - puuP MCAODC_15820 0.15 -0.6
56,011 - puuP MCAODC_15820 0.21 -0.8
56,032 + puuP MCAODC_15820 0.22 -0.3
56,159 + puuP MCAODC_15820 0.31 -0.2
56,159 + puuP MCAODC_15820 0.31 +0.5
56,160 - puuP MCAODC_15820 0.32 +1.5
56,261 + puuP MCAODC_15820 0.39 -2.3
56,331 + puuP MCAODC_15820 0.44 +0.7
56,366 + puuP MCAODC_15820 0.46 +0.3
56,366 + puuP MCAODC_15820 0.46 -0.6
56,367 - puuP MCAODC_15820 0.46 +0.6
56,517 + puuP MCAODC_15820 0.57 +0.2
56,560 - puuP MCAODC_15820 0.60 +1.3
56,586 + puuP MCAODC_15820 0.62 +0.9

Or see this region's nucleotide sequence