Experiment: WM_TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycgM and maiA are separated by 8 nucleotides maiA and ubiH are separated by 14 nucleotides ubiH and dusC are separated by 158 nucleotides
MCAODC_14650: ycgM - 5-carboxymethyl-2-hydroxymuconate isomerase, at 2,885,681 to 2,886,382
ycgM
MCAODC_14655: maiA - maleylacetoacetate isomerase, at 2,886,391 to 2,887,035
maiA
MCAODC_14660: ubiH - 3-hydroxybenzoate 6-monooxygenase, at 2,887,050 to 2,888,243
ubiH
MCAODC_14665: dusC - tRNA dihydrouridine(16) synthase DusC, at 2,888,402 to 2,889,352
dusC
Position (kb)
2887
2888
2889 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2886.152 kb on - strand, within ycgM at 2886.152 kb on - strand, within ycgM at 2886.203 kb on - strand, within ycgM at 2886.415 kb on - strand at 2886.415 kb on - strand at 2886.462 kb on + strand, within maiA at 2886.597 kb on + strand, within maiA at 2886.598 kb on - strand, within maiA at 2886.683 kb on - strand, within maiA at 2886.683 kb on - strand, within maiA at 2886.961 kb on + strand, within maiA at 2886.962 kb on - strand, within maiA at 2887.021 kb on - strand at 2887.046 kb on + strand at 2887.046 kb on + strand at 2887.047 kb on - strand at 2887.047 kb on - strand at 2887.047 kb on - strand at 2887.047 kb on - strand at 2887.047 kb on - strand at 2887.047 kb on - strand at 2887.072 kb on + strand at 2887.072 kb on + strand at 2887.274 kb on + strand, within ubiH at 2887.502 kb on + strand, within ubiH at 2887.564 kb on + strand, within ubiH at 2887.602 kb on + strand, within ubiH at 2887.661 kb on - strand, within ubiH at 2887.661 kb on - strand, within ubiH at 2887.661 kb on - strand, within ubiH at 2887.719 kb on + strand, within ubiH at 2887.719 kb on + strand, within ubiH at 2887.719 kb on + strand, within ubiH at 2887.720 kb on - strand, within ubiH at 2887.721 kb on + strand, within ubiH at 2887.733 kb on - strand, within ubiH at 2887.778 kb on + strand, within ubiH at 2887.778 kb on + strand, within ubiH at 2887.779 kb on - strand, within ubiH at 2887.779 kb on - strand, within ubiH at 2887.779 kb on - strand, within ubiH at 2887.781 kb on + strand, within ubiH at 2887.781 kb on + strand, within ubiH at 2887.781 kb on + strand, within ubiH at 2887.781 kb on + strand, within ubiH at 2887.782 kb on - strand, within ubiH at 2887.782 kb on - strand, within ubiH at 2887.800 kb on + strand, within ubiH at 2887.800 kb on + strand, within ubiH at 2887.801 kb on - strand, within ubiH at 2887.950 kb on + strand, within ubiH at 2887.950 kb on + strand, within ubiH at 2887.951 kb on - strand, within ubiH at 2887.951 kb on - strand, within ubiH at 2887.951 kb on - strand, within ubiH at 2887.951 kb on - strand, within ubiH at 2887.952 kb on + strand, within ubiH at 2887.952 kb on + strand, within ubiH at 2887.952 kb on + strand, within ubiH at 2887.953 kb on - strand, within ubiH at 2887.953 kb on - strand, within ubiH at 2887.971 kb on - strand, within ubiH at 2887.971 kb on - strand, within ubiH at 2888.001 kb on - strand, within ubiH at 2888.132 kb on - strand at 2888.143 kb on + strand at 2888.143 kb on + strand at 2888.144 kb on - strand at 2888.144 kb on - strand at 2888.147 kb on + strand at 2888.147 kb on + strand at 2888.148 kb on - strand at 2888.205 kb on + strand at 2888.205 kb on + strand at 2888.222 kb on + strand at 2888.301 kb on + strand at 2888.337 kb on + strand at 2888.338 kb on - strand at 2888.375 kb on - strand at 2888.478 kb on - strand at 2888.478 kb on - strand at 2888.478 kb on - strand at 2888.478 kb on - strand at 2888.559 kb on + strand, within dusC at 2888.661 kb on + strand, within dusC at 2888.661 kb on + strand, within dusC at 2888.662 kb on - strand, within dusC at 2888.759 kb on + strand, within dusC at 2888.760 kb on - strand, within dusC at 2888.942 kb on - strand, within dusC at 2889.088 kb on + strand, within dusC at 2889.088 kb on + strand, within dusC at 2889.111 kb on + strand, within dusC at 2889.111 kb on + strand, within dusC at 2889.202 kb on - strand, within dusC at 2889.203 kb on + strand, within dusC at 2889.203 kb on + strand, within dusC at 2889.204 kb on - strand, within dusC at 2889.237 kb on - strand, within dusC at 2889.237 kb on - strand, within dusC
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP9 remove 2,886,152 - ycgM MCAODC_14650 0.67 +0.2 2,886,152 - ycgM MCAODC_14650 0.67 +0.4 2,886,203 - ycgM MCAODC_14650 0.74 -0.2 2,886,415 - -0.3 2,886,415 - -0.6 2,886,462 + maiA MCAODC_14655 0.11 +0.6 2,886,597 + maiA MCAODC_14655 0.32 +0.6 2,886,598 - maiA MCAODC_14655 0.32 +0.4 2,886,683 - maiA MCAODC_14655 0.45 +0.4 2,886,683 - maiA MCAODC_14655 0.45 -0.6 2,886,961 + maiA MCAODC_14655 0.88 +0.5 2,886,962 - maiA MCAODC_14655 0.89 -2.4 2,887,021 - +1.7 2,887,046 + +0.6 2,887,046 + +0.2 2,887,047 - +0.0 2,887,047 - -0.4 2,887,047 - -0.5 2,887,047 - +0.7 2,887,047 - +0.1 2,887,047 - +1.1 2,887,072 + +0.1 2,887,072 + -2.3 2,887,274 + ubiH MCAODC_14660 0.19 +0.0 2,887,502 + ubiH MCAODC_14660 0.38 +0.4 2,887,564 + ubiH MCAODC_14660 0.43 +1.4 2,887,602 + ubiH MCAODC_14660 0.46 +0.7 2,887,661 - ubiH MCAODC_14660 0.51 +1.2 2,887,661 - ubiH MCAODC_14660 0.51 -0.0 2,887,661 - ubiH MCAODC_14660 0.51 -0.5 2,887,719 + ubiH MCAODC_14660 0.56 -0.2 2,887,719 + ubiH MCAODC_14660 0.56 -0.4 2,887,719 + ubiH MCAODC_14660 0.56 +1.1 2,887,720 - ubiH MCAODC_14660 0.56 +0.8 2,887,721 + ubiH MCAODC_14660 0.56 +1.8 2,887,733 - ubiH MCAODC_14660 0.57 -0.7 2,887,778 + ubiH MCAODC_14660 0.61 +0.1 2,887,778 + ubiH MCAODC_14660 0.61 -0.4 2,887,779 - ubiH MCAODC_14660 0.61 +0.2 2,887,779 - ubiH MCAODC_14660 0.61 +0.5 2,887,779 - ubiH MCAODC_14660 0.61 +1.2 2,887,781 + ubiH MCAODC_14660 0.61 +0.3 2,887,781 + ubiH MCAODC_14660 0.61 -0.1 2,887,781 + ubiH MCAODC_14660 0.61 -0.0 2,887,781 + ubiH MCAODC_14660 0.61 +0.5 2,887,782 - ubiH MCAODC_14660 0.61 +0.7 2,887,782 - ubiH MCAODC_14660 0.61 -0.2 2,887,800 + ubiH MCAODC_14660 0.63 +0.7 2,887,800 + ubiH MCAODC_14660 0.63 +0.6 2,887,801 - ubiH MCAODC_14660 0.63 +0.0 2,887,950 + ubiH MCAODC_14660 0.75 -0.1 2,887,950 + ubiH MCAODC_14660 0.75 +0.1 2,887,951 - ubiH MCAODC_14660 0.75 -0.2 2,887,951 - ubiH MCAODC_14660 0.75 -0.8 2,887,951 - ubiH MCAODC_14660 0.75 -1.9 2,887,951 - ubiH MCAODC_14660 0.75 -0.6 2,887,952 + ubiH MCAODC_14660 0.76 +0.4 2,887,952 + ubiH MCAODC_14660 0.76 -1.0 2,887,952 + ubiH MCAODC_14660 0.76 -0.8 2,887,953 - ubiH MCAODC_14660 0.76 -0.2 2,887,953 - ubiH MCAODC_14660 0.76 -0.4 2,887,971 - ubiH MCAODC_14660 0.77 -0.1 2,887,971 - ubiH MCAODC_14660 0.77 +0.2 2,888,001 - ubiH MCAODC_14660 0.80 -0.0 2,888,132 - +1.6 2,888,143 + -0.3 2,888,143 + -0.3 2,888,144 - +0.3 2,888,144 - -3.3 2,888,147 + -0.4 2,888,147 + +0.3 2,888,148 - +1.3 2,888,205 + +1.0 2,888,205 + -0.2 2,888,222 + +2.1 2,888,301 + +0.1 2,888,337 + +1.2 2,888,338 - -1.0 2,888,375 - -0.2 2,888,478 - +0.4 2,888,478 - +1.0 2,888,478 - -0.9 2,888,478 - +0.5 2,888,559 + dusC MCAODC_14665 0.17 -0.2 2,888,661 + dusC MCAODC_14665 0.27 +1.0 2,888,661 + dusC MCAODC_14665 0.27 +0.5 2,888,662 - dusC MCAODC_14665 0.27 +0.5 2,888,759 + dusC MCAODC_14665 0.38 -0.7 2,888,760 - dusC MCAODC_14665 0.38 +0.4 2,888,942 - dusC MCAODC_14665 0.57 +0.3 2,889,088 + dusC MCAODC_14665 0.72 +0.7 2,889,088 + dusC MCAODC_14665 0.72 -0.6 2,889,111 + dusC MCAODC_14665 0.75 -0.0 2,889,111 + dusC MCAODC_14665 0.75 +0.4 2,889,202 - dusC MCAODC_14665 0.84 +0.8 2,889,203 + dusC MCAODC_14665 0.84 +0.5 2,889,203 + dusC MCAODC_14665 0.84 -0.7 2,889,204 - dusC MCAODC_14665 0.84 +1.7 2,889,237 - dusC MCAODC_14665 0.88 -0.6 2,889,237 - dusC MCAODC_14665 0.88 +1.2
Or see this region's nucleotide sequence