Experiment: WM_TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lptF and pepA are separated by 266 nucleotides pepA and holC are separated by 353 nucleotides holC and valS overlap by 1 nucleotides
MCAODC_02290: lptF - LPS export ABC transporter permease LptF, at 471,209 to 472,309
lptF
MCAODC_02295: pepA - leucyl aminopeptidase, at 472,576 to 474,087
pepA
MCAODC_02315: holC - DNA polymerase III subunit chi, at 474,441 to 474,884
holC
MCAODC_02320: valS - valine--tRNA ligase, at 474,884 to 477,739
valS
Position (kb) 472
473
474
475 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 472.367 kb on - strand at 472.410 kb on + strand at 472.410 kb on + strand at 472.411 kb on - strand at 472.411 kb on - strand at 472.415 kb on - strand at 472.415 kb on - strand at 472.415 kb on - strand at 472.417 kb on - strand at 472.417 kb on - strand at 472.465 kb on - strand at 472.474 kb on - strand at 472.513 kb on - strand at 472.536 kb on + strand at 472.536 kb on + strand at 472.570 kb on + strand at 472.570 kb on + strand at 472.570 kb on + strand at 472.570 kb on + strand at 472.584 kb on + strand at 472.585 kb on - strand at 472.585 kb on - strand at 472.585 kb on - strand at 472.589 kb on + strand at 472.699 kb on + strand at 472.700 kb on - strand at 472.700 kb on - strand at 472.700 kb on - strand at 472.715 kb on + strand at 472.715 kb on + strand at 472.715 kb on + strand at 472.778 kb on + strand, within pepA at 472.779 kb on - strand, within pepA at 472.840 kb on + strand, within pepA at 472.840 kb on + strand, within pepA at 472.841 kb on - strand, within pepA at 472.870 kb on - strand, within pepA at 472.870 kb on - strand, within pepA at 472.870 kb on - strand, within pepA at 472.870 kb on - strand, within pepA at 472.988 kb on - strand, within pepA at 473.040 kb on + strand, within pepA at 473.040 kb on + strand, within pepA at 473.152 kb on - strand, within pepA at 473.163 kb on + strand, within pepA at 473.215 kb on + strand, within pepA at 473.215 kb on + strand, within pepA at 473.216 kb on - strand, within pepA at 473.229 kb on + strand, within pepA at 473.230 kb on - strand, within pepA at 473.281 kb on + strand, within pepA at 473.281 kb on + strand, within pepA at 473.390 kb on + strand, within pepA at 473.409 kb on + strand, within pepA at 473.449 kb on + strand, within pepA at 473.449 kb on + strand, within pepA at 473.449 kb on + strand, within pepA at 473.450 kb on - strand, within pepA at 473.454 kb on + strand, within pepA at 473.454 kb on + strand, within pepA at 473.454 kb on + strand, within pepA at 473.454 kb on + strand, within pepA at 473.455 kb on - strand, within pepA at 473.505 kb on - strand, within pepA at 473.505 kb on - strand, within pepA at 473.569 kb on + strand, within pepA at 473.588 kb on + strand, within pepA at 473.648 kb on + strand, within pepA at 473.649 kb on - strand, within pepA at 473.668 kb on + strand, within pepA at 473.668 kb on + strand, within pepA at 473.668 kb on + strand, within pepA at 473.668 kb on + strand, within pepA at 473.669 kb on - strand, within pepA at 473.772 kb on + strand, within pepA at 473.772 kb on + strand, within pepA at 473.773 kb on - strand, within pepA at 473.851 kb on + strand, within pepA at 473.946 kb on + strand at 473.952 kb on + strand at 473.953 kb on - strand at 473.956 kb on + strand at 473.956 kb on + strand at 473.957 kb on - strand at 473.957 kb on - strand at 473.957 kb on - strand at 473.977 kb on - strand at 474.085 kb on + strand at 474.085 kb on + strand at 474.085 kb on + strand at 474.086 kb on - strand at 474.142 kb on + strand at 474.161 kb on - strand at 474.204 kb on - strand at 474.239 kb on - strand at 474.239 kb on - strand at 474.299 kb on + strand at 474.334 kb on + strand at 474.334 kb on + strand at 474.334 kb on + strand at 474.335 kb on - strand at 474.335 kb on - strand at 474.363 kb on + strand at 474.364 kb on - strand at 474.398 kb on + strand at 474.398 kb on + strand at 474.398 kb on + strand at 474.399 kb on - strand at 474.399 kb on - strand at 474.400 kb on + strand at 474.401 kb on - strand at 474.411 kb on + strand at 474.411 kb on + strand at 474.412 kb on - strand at 474.415 kb on + strand at 474.415 kb on + strand at 474.416 kb on - strand at 474.416 kb on - strand at 474.416 kb on - strand at 474.416 kb on - strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.441 kb on - strand at 474.459 kb on + strand at 474.459 kb on + strand at 474.459 kb on + strand at 474.460 kb on - strand at 474.460 kb on - strand at 474.588 kb on + strand, within holC at 474.647 kb on - strand, within holC at 474.722 kb on + strand, within holC at 474.723 kb on - strand, within holC at 474.725 kb on - strand, within holC at 474.831 kb on + strand, within holC
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP9 remove 472,367 - +1.9 472,410 + +0.4 472,410 + -0.6 472,411 - -2.2 472,411 - +0.3 472,415 - -0.8 472,415 - +0.2 472,415 - +1.4 472,417 - -0.4 472,417 - +1.2 472,465 - -1.5 472,474 - +0.3 472,513 - -0.0 472,536 + +0.5 472,536 + +0.3 472,570 + -0.9 472,570 + -0.6 472,570 + -0.4 472,570 + +0.4 472,584 + +0.4 472,585 - +0.5 472,585 - -0.1 472,585 - -0.9 472,589 + +0.5 472,699 + -3.2 472,700 - +0.4 472,700 - +0.6 472,700 - +0.1 472,715 + -0.0 472,715 + -0.5 472,715 + +0.9 472,778 + pepA MCAODC_02295 0.13 -0.2 472,779 - pepA MCAODC_02295 0.13 +0.8 472,840 + pepA MCAODC_02295 0.17 +0.8 472,840 + pepA MCAODC_02295 0.17 +0.7 472,841 - pepA MCAODC_02295 0.18 -2.0 472,870 - pepA MCAODC_02295 0.19 +0.5 472,870 - pepA MCAODC_02295 0.19 +0.8 472,870 - pepA MCAODC_02295 0.19 +1.0 472,870 - pepA MCAODC_02295 0.19 +0.7 472,988 - pepA MCAODC_02295 0.27 +0.2 473,040 + pepA MCAODC_02295 0.31 +0.4 473,040 + pepA MCAODC_02295 0.31 +0.4 473,152 - pepA MCAODC_02295 0.38 -0.0 473,163 + pepA MCAODC_02295 0.39 +0.0 473,215 + pepA MCAODC_02295 0.42 -1.2 473,215 + pepA MCAODC_02295 0.42 -0.3 473,216 - pepA MCAODC_02295 0.42 +0.5 473,229 + pepA MCAODC_02295 0.43 +0.3 473,230 - pepA MCAODC_02295 0.43 -0.5 473,281 + pepA MCAODC_02295 0.47 -0.0 473,281 + pepA MCAODC_02295 0.47 -0.7 473,390 + pepA MCAODC_02295 0.54 +0.4 473,409 + pepA MCAODC_02295 0.55 +0.2 473,449 + pepA MCAODC_02295 0.58 -0.2 473,449 + pepA MCAODC_02295 0.58 -2.2 473,449 + pepA MCAODC_02295 0.58 -0.4 473,450 - pepA MCAODC_02295 0.58 -0.7 473,454 + pepA MCAODC_02295 0.58 +0.4 473,454 + pepA MCAODC_02295 0.58 -0.9 473,454 + pepA MCAODC_02295 0.58 -0.2 473,454 + pepA MCAODC_02295 0.58 +1.5 473,455 - pepA MCAODC_02295 0.58 -0.2 473,505 - pepA MCAODC_02295 0.61 +0.3 473,505 - pepA MCAODC_02295 0.61 +0.7 473,569 + pepA MCAODC_02295 0.66 -0.0 473,588 + pepA MCAODC_02295 0.67 -0.7 473,648 + pepA MCAODC_02295 0.71 -1.2 473,649 - pepA MCAODC_02295 0.71 -0.1 473,668 + pepA MCAODC_02295 0.72 -1.2 473,668 + pepA MCAODC_02295 0.72 -0.2 473,668 + pepA MCAODC_02295 0.72 +0.3 473,668 + pepA MCAODC_02295 0.72 -1.6 473,669 - pepA MCAODC_02295 0.72 -0.4 473,772 + pepA MCAODC_02295 0.79 -0.2 473,772 + pepA MCAODC_02295 0.79 +0.5 473,773 - pepA MCAODC_02295 0.79 +1.7 473,851 + pepA MCAODC_02295 0.84 +1.0 473,946 + +0.5 473,952 + -0.3 473,953 - +0.1 473,956 + -1.0 473,956 + +0.4 473,957 - -2.7 473,957 - +0.5 473,957 - +0.6 473,977 - +0.6 474,085 + +0.8 474,085 + -0.0 474,085 + -1.0 474,086 - +0.1 474,142 + -0.1 474,161 - +1.0 474,204 - -0.5 474,239 - +0.3 474,239 - +0.8 474,299 + +0.2 474,334 + +1.3 474,334 + -0.2 474,334 + -0.0 474,335 - +0.4 474,335 - -0.7 474,363 + +0.1 474,364 - -0.3 474,398 + +0.4 474,398 + +0.8 474,398 + -0.4 474,399 - -0.6 474,399 - -0.2 474,400 + +0.3 474,401 - -0.1 474,411 + +0.3 474,411 + +0.8 474,412 - -0.0 474,415 + -0.4 474,415 + +1.9 474,416 - +0.6 474,416 - +1.2 474,416 - -0.4 474,416 - +0.0 474,438 + +0.1 474,438 + +0.2 474,438 + -0.1 474,438 + +0.5 474,438 + +0.4 474,439 - +1.0 474,439 - +0.9 474,439 - -1.2 474,439 - +0.8 474,439 - -3.3 474,439 - +0.0 474,440 + -0.3 474,440 + +0.0 474,440 + +0.0 474,440 + +0.2 474,440 + -0.2 474,440 + -0.2 474,440 + -0.5 474,440 + +0.1 474,441 - -0.2 474,459 + +0.8 474,459 + -0.0 474,459 + -0.2 474,460 - +0.3 474,460 - -1.0 474,588 + holC MCAODC_02315 0.33 -0.0 474,647 - holC MCAODC_02315 0.46 -1.1 474,722 + holC MCAODC_02315 0.63 +0.2 474,723 - holC MCAODC_02315 0.64 -0.8 474,725 - holC MCAODC_02315 0.64 -0.1 474,831 + holC MCAODC_02315 0.88 -1.4
Or see this region's nucleotide sequence