Strain Fitness in Escherichia coli ECRC101 around MCAODC_00650

Experiment: WM_TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaccA and dnaE are separated by 12 nucleotidesdnaE and rnhB are separated by 36 nucleotidesrnhB and lpxB overlap by 4 nucleotides MCAODC_00645: accA - acetyl-CoA carboxylase carboxyl transferase subunit alpha, at 104,287 to 105,246 accA MCAODC_00650: dnaE - DNA polymerase III subunit alpha, at 105,259 to 108,741 dnaE MCAODC_00655: rnhB - ribonuclease HII, at 108,778 to 109,374 rnhB MCAODC_00660: lpxB - lipid-A-disaccharide synthase, at 109,371 to 110,519 lpxB Position (kb) 105 106 107 108 109Strain fitness (log2 ratio) -1 0 1at 108.758 kb on - strandat 108.762 kb on - strandat 108.762 kb on - strandat 108.906 kb on - strand, within rnhBat 109.065 kb on - strand, within rnhBat 109.124 kb on - strand, within rnhBat 109.307 kb on - strand, within rnhB

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP9
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108,758 - +0.2
108,762 - +0.8
108,762 - +0.6
108,906 - rnhB MCAODC_00655 0.21 +0.5
109,065 - rnhB MCAODC_00655 0.48 +1.0
109,124 - rnhB MCAODC_00655 0.58 +0.2
109,307 - rnhB MCAODC_00655 0.89 +1.0

Or see this region's nucleotide sequence