Strain Fitness in Escherichia coli ECRC101 around MCAODC_24335

Experiment: WM_TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_24325 and tnpB are separated by 49 nucleotidestnpB and tnpB overlap by 4 nucleotidestnpB and nu1 are separated by 75 nucleotides MCAODC_24325: MCAODC_24325 - IS66-like element ISEc8 family transposase, at 1,590,701 to 1,592,239 _24325 MCAODC_24330: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 1,592,289 to 1,592,636 tnpB MCAODC_24335: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 1,592,633 to 1,593,013 tnpB MCAODC_24340: nu1 - Prophage Qin DNA packaging protein NU1-like protein, at 1,593,089 to 1,593,364 nu1 Position (kb) 1592 1593 1594Strain fitness (log2 ratio) -2 -1 0 1at 1593.097 kb on + strandat 1593.799 kb on - strandat 1593.810 kb on - strandat 1593.810 kb on - strandat 1593.939 kb on + strandat 1593.939 kb on + strandat 1593.951 kb on + strandat 1593.951 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP9
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1,593,097 + -0.8
1,593,799 - -1.5
1,593,810 - -1.2
1,593,810 - +1.0
1,593,939 + -2.0
1,593,939 + +0.3
1,593,951 + -1.3
1,593,951 + +0.6

Or see this region's nucleotide sequence