Strain Fitness in Escherichia coli ECRC101 around MCAODC_22335

Experiment: WM_TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nticd and rluE are separated by 171 nucleotidesrluE and nudJ are separated by 9 nucleotidesnudJ and mnmA are separated by 53 nucleotides MCAODC_22325: icd - NADP-dependent isocitrate dehydrogenase, at 1,229,302 to 1,230,552 icd MCAODC_22330: rluE - 23S rRNA pseudouridine(2457) synthase RluE, at 1,230,724 to 1,231,377 rluE MCAODC_22335: nudJ - phosphatase NudJ, at 1,231,387 to 1,231,848 nudJ MCAODC_22340: mnmA - tRNA 2-thiouridine(34) synthase MnmA, at 1,231,902 to 1,233,008 mnmA Position (kb) 1231 1232Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1230.713 kb on - strandat 1230.716 kb on - strandat 1230.721 kb on - strandat 1230.721 kb on - strandat 1230.721 kb on - strandat 1230.858 kb on + strand, within rluEat 1230.865 kb on + strand, within rluEat 1230.866 kb on - strand, within rluEat 1230.911 kb on + strand, within rluEat 1231.048 kb on + strand, within rluEat 1231.048 kb on + strand, within rluEat 1231.049 kb on - strand, within rluEat 1231.051 kb on + strand, within rluEat 1231.051 kb on + strand, within rluEat 1231.051 kb on + strand, within rluEat 1231.052 kb on - strand, within rluEat 1231.068 kb on + strand, within rluEat 1231.109 kb on + strand, within rluEat 1231.115 kb on + strand, within rluEat 1231.116 kb on - strand, within rluEat 1231.172 kb on + strand, within rluEat 1231.238 kb on + strand, within rluEat 1231.238 kb on + strand, within rluEat 1231.238 kb on + strand, within rluEat 1231.239 kb on - strand, within rluEat 1231.239 kb on - strand, within rluEat 1231.240 kb on + strand, within rluEat 1231.240 kb on + strand, within rluEat 1231.240 kb on + strand, within rluEat 1231.240 kb on + strand, within rluEat 1231.240 kb on + strand, within rluEat 1231.240 kb on + strand, within rluEat 1231.241 kb on - strand, within rluEat 1231.241 kb on - strand, within rluEat 1231.241 kb on - strand, within rluEat 1231.241 kb on - strand, within rluEat 1231.251 kb on + strand, within rluEat 1231.327 kb on + strandat 1231.375 kb on + strandat 1231.467 kb on + strand, within nudJat 1231.518 kb on - strand, within nudJat 1231.659 kb on - strand, within nudJat 1231.764 kb on + strand, within nudJ

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP9
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1,230,713 - -0.7
1,230,716 - +0.2
1,230,721 - -0.3
1,230,721 - +0.8
1,230,721 - -2.8
1,230,858 + rluE MCAODC_22330 0.20 +0.9
1,230,865 + rluE MCAODC_22330 0.22 +0.6
1,230,866 - rluE MCAODC_22330 0.22 +0.2
1,230,911 + rluE MCAODC_22330 0.29 +2.5
1,231,048 + rluE MCAODC_22330 0.50 +0.0
1,231,048 + rluE MCAODC_22330 0.50 +0.8
1,231,049 - rluE MCAODC_22330 0.50 +1.1
1,231,051 + rluE MCAODC_22330 0.50 +0.8
1,231,051 + rluE MCAODC_22330 0.50 -0.6
1,231,051 + rluE MCAODC_22330 0.50 -0.4
1,231,052 - rluE MCAODC_22330 0.50 -0.8
1,231,068 + rluE MCAODC_22330 0.53 -0.9
1,231,109 + rluE MCAODC_22330 0.59 +0.4
1,231,115 + rluE MCAODC_22330 0.60 +1.0
1,231,116 - rluE MCAODC_22330 0.60 -2.6
1,231,172 + rluE MCAODC_22330 0.69 -0.0
1,231,238 + rluE MCAODC_22330 0.79 -0.3
1,231,238 + rluE MCAODC_22330 0.79 -2.1
1,231,238 + rluE MCAODC_22330 0.79 +0.7
1,231,239 - rluE MCAODC_22330 0.79 -0.4
1,231,239 - rluE MCAODC_22330 0.79 -0.7
1,231,240 + rluE MCAODC_22330 0.79 -1.2
1,231,240 + rluE MCAODC_22330 0.79 -2.4
1,231,240 + rluE MCAODC_22330 0.79 -0.0
1,231,240 + rluE MCAODC_22330 0.79 +0.3
1,231,240 + rluE MCAODC_22330 0.79 +1.9
1,231,240 + rluE MCAODC_22330 0.79 -0.2
1,231,241 - rluE MCAODC_22330 0.79 +0.8
1,231,241 - rluE MCAODC_22330 0.79 -0.0
1,231,241 - rluE MCAODC_22330 0.79 +0.7
1,231,241 - rluE MCAODC_22330 0.79 -0.4
1,231,251 + rluE MCAODC_22330 0.81 +1.2
1,231,327 + +0.2
1,231,375 + -0.2
1,231,467 + nudJ MCAODC_22335 0.17 +0.2
1,231,518 - nudJ MCAODC_22335 0.28 +1.2
1,231,659 - nudJ MCAODC_22335 0.59 +3.1
1,231,764 + nudJ MCAODC_22335 0.82 +0.5

Or see this region's nucleotide sequence