Experiment: WM_TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt icd and rluE are separated by 171 nucleotides rluE and nudJ are separated by 9 nucleotides nudJ and mnmA are separated by 53 nucleotides
MCAODC_22325: icd - NADP-dependent isocitrate dehydrogenase, at 1,229,302 to 1,230,552
icd
MCAODC_22330: rluE - 23S rRNA pseudouridine(2457) synthase RluE, at 1,230,724 to 1,231,377
rluE
MCAODC_22335: nudJ - phosphatase NudJ, at 1,231,387 to 1,231,848
nudJ
MCAODC_22340: mnmA - tRNA 2-thiouridine(34) synthase MnmA, at 1,231,902 to 1,233,008
mnmA
Position (kb)
1231
1232 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1230.713 kb on - strand at 1230.716 kb on - strand at 1230.721 kb on - strand at 1230.721 kb on - strand at 1230.721 kb on - strand at 1230.858 kb on + strand, within rluE at 1230.865 kb on + strand, within rluE at 1230.866 kb on - strand, within rluE at 1230.911 kb on + strand, within rluE at 1231.048 kb on + strand, within rluE at 1231.048 kb on + strand, within rluE at 1231.049 kb on - strand, within rluE at 1231.051 kb on + strand, within rluE at 1231.051 kb on + strand, within rluE at 1231.051 kb on + strand, within rluE at 1231.052 kb on - strand, within rluE at 1231.068 kb on + strand, within rluE at 1231.109 kb on + strand, within rluE at 1231.115 kb on + strand, within rluE at 1231.116 kb on - strand, within rluE at 1231.172 kb on + strand, within rluE at 1231.238 kb on + strand, within rluE at 1231.238 kb on + strand, within rluE at 1231.238 kb on + strand, within rluE at 1231.239 kb on - strand, within rluE at 1231.239 kb on - strand, within rluE at 1231.240 kb on + strand, within rluE at 1231.240 kb on + strand, within rluE at 1231.240 kb on + strand, within rluE at 1231.240 kb on + strand, within rluE at 1231.240 kb on + strand, within rluE at 1231.240 kb on + strand, within rluE at 1231.241 kb on - strand, within rluE at 1231.241 kb on - strand, within rluE at 1231.241 kb on - strand, within rluE at 1231.241 kb on - strand, within rluE at 1231.251 kb on + strand, within rluE at 1231.327 kb on + strand at 1231.375 kb on + strand at 1231.467 kb on + strand, within nudJ at 1231.518 kb on - strand, within nudJ at 1231.659 kb on - strand, within nudJ at 1231.764 kb on + strand, within nudJ
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP9 remove 1,230,713 - -0.7 1,230,716 - +0.2 1,230,721 - -0.3 1,230,721 - +0.8 1,230,721 - -2.8 1,230,858 + rluE MCAODC_22330 0.20 +0.9 1,230,865 + rluE MCAODC_22330 0.22 +0.6 1,230,866 - rluE MCAODC_22330 0.22 +0.2 1,230,911 + rluE MCAODC_22330 0.29 +2.5 1,231,048 + rluE MCAODC_22330 0.50 +0.0 1,231,048 + rluE MCAODC_22330 0.50 +0.8 1,231,049 - rluE MCAODC_22330 0.50 +1.1 1,231,051 + rluE MCAODC_22330 0.50 +0.8 1,231,051 + rluE MCAODC_22330 0.50 -0.6 1,231,051 + rluE MCAODC_22330 0.50 -0.4 1,231,052 - rluE MCAODC_22330 0.50 -0.8 1,231,068 + rluE MCAODC_22330 0.53 -0.9 1,231,109 + rluE MCAODC_22330 0.59 +0.4 1,231,115 + rluE MCAODC_22330 0.60 +1.0 1,231,116 - rluE MCAODC_22330 0.60 -2.6 1,231,172 + rluE MCAODC_22330 0.69 -0.0 1,231,238 + rluE MCAODC_22330 0.79 -0.3 1,231,238 + rluE MCAODC_22330 0.79 -2.1 1,231,238 + rluE MCAODC_22330 0.79 +0.7 1,231,239 - rluE MCAODC_22330 0.79 -0.4 1,231,239 - rluE MCAODC_22330 0.79 -0.7 1,231,240 + rluE MCAODC_22330 0.79 -1.2 1,231,240 + rluE MCAODC_22330 0.79 -2.4 1,231,240 + rluE MCAODC_22330 0.79 -0.0 1,231,240 + rluE MCAODC_22330 0.79 +0.3 1,231,240 + rluE MCAODC_22330 0.79 +1.9 1,231,240 + rluE MCAODC_22330 0.79 -0.2 1,231,241 - rluE MCAODC_22330 0.79 +0.8 1,231,241 - rluE MCAODC_22330 0.79 -0.0 1,231,241 - rluE MCAODC_22330 0.79 +0.7 1,231,241 - rluE MCAODC_22330 0.79 -0.4 1,231,251 + rluE MCAODC_22330 0.81 +1.2 1,231,327 + +0.2 1,231,375 + -0.2 1,231,467 + nudJ MCAODC_22335 0.17 +0.2 1,231,518 - nudJ MCAODC_22335 0.28 +1.2 1,231,659 - nudJ MCAODC_22335 0.59 +3.1 1,231,764 + nudJ MCAODC_22335 0.82 +0.5
Or see this region's nucleotide sequence