Strain Fitness in Escherichia coli ECRC101 around MCAODC_22320

Experiment: WM_TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_22310 and xis are separated by 103 nucleotidesxis and int overlap by 11 nucleotidesint and icd are separated by 113 nucleotides MCAODC_22310: MCAODC_22310 - replication protein, at 1,226,892 to 1,227,716 _22310 MCAODC_22315: xis - Excisionase, at 1,227,820 to 1,228,056 xis MCAODC_22320: int - Integrase, at 1,228,046 to 1,229,188 int MCAODC_22325: icd - NADP-dependent isocitrate dehydrogenase, at 1,229,302 to 1,230,552 icd Position (kb) 1228 1229 1230Strain fitness (log2 ratio) -2 -1 0 1 2at 1227.729 kb on + strandat 1227.806 kb on + strandat 1227.806 kb on + strandat 1227.872 kb on + strand, within xisat 1228.123 kb on - strandat 1228.354 kb on + strand, within intat 1228.363 kb on - strand, within intat 1228.786 kb on + strand, within intat 1228.786 kb on + strand, within intat 1228.852 kb on + strand, within intat 1229.067 kb on + strand, within intat 1229.069 kb on + strand, within intat 1229.288 kb on - strandat 1229.288 kb on - strandat 1229.297 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP9
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1,227,729 + -0.7
1,227,806 + -1.0
1,227,806 + +0.9
1,227,872 + xis MCAODC_22315 0.22 -1.5
1,228,123 - +0.2
1,228,354 + int MCAODC_22320 0.27 -0.2
1,228,363 - int MCAODC_22320 0.28 +0.2
1,228,786 + int MCAODC_22320 0.65 -0.1
1,228,786 + int MCAODC_22320 0.65 +1.0
1,228,852 + int MCAODC_22320 0.71 +1.5
1,229,067 + int MCAODC_22320 0.89 +1.8
1,229,069 + int MCAODC_22320 0.90 +1.2
1,229,288 - +0.9
1,229,288 - -2.5
1,229,297 - -0.0

Or see this region's nucleotide sequence