Strain Fitness in Escherichia coli ECRC101 around MCAODC_20500

Experiment: WM_TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwbdQ and wcaG are separated by 2 nucleotideswcaG and MCAODC_20500 are separated by 2 nucleotidesMCAODC_20500 and rfaB are separated by 19 nucleotides MCAODC_20490: wbdQ - GDP-mannose mannosyl hydrolase WbdQ/WbhG, at 906,154 to 906,663 wbdQ MCAODC_20495: wcaG - GDP-fucose synthetase, at 906,666 to 907,631 wcaG MCAODC_20500: MCAODC_20500 - GDP-mannose 4,6-dehydratase, at 907,634 to 908,752 _20500 MCAODC_20505: rfaB - glycosyl transferase, at 908,772 to 909,986 rfaB Position (kb) 907 908 909Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 906.991 kb on - strand, within wcaGat 907.226 kb on - strand, within wcaGat 907.893 kb on - strand, within MCAODC_20500at 909.117 kb on - strand, within rfaBat 909.674 kb on - strand, within rfaB

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP9
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906,991 - wcaG MCAODC_20495 0.34 -1.3
907,226 - wcaG MCAODC_20495 0.58 -1.8
907,893 - MCAODC_20500 0.23 -1.0
909,117 - rfaB MCAODC_20505 0.28 +2.8
909,674 - rfaB MCAODC_20505 0.74 +5.4

Or see this region's nucleotide sequence