Experiment: WM_TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Leu_trna and Cys_trna are separated by 12 nucleotides Cys_trna and Gly_trna are separated by 53 nucleotides Gly_trna and pgsA are separated by 151 nucleotides pgsA and uvrC are separated by 56 nucleotides uvrC and uvrC overlap by 4 nucleotides
MCAODC_19545: Leu_trna - tRNA-Leu(taa), at 743,357 to 743,443
_trna
MCAODC_19550: Cys_trna - tRNA-Cys(gca), at 743,456 to 743,529
_trna
MCAODC_19555: Gly_trna - tRNA-Gly(gcc), at 743,583 to 743,658
_trna
MCAODC_19560: pgsA - CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase, at 743,810 to 744,358
pgsA
MCAODC_19565: uvrC - excinuclease ABC subunit UvrC, at 744,415 to 746,247
uvrC
MCAODC_19570: uvrC - excinuclease ABC subunit UvrC, at 746,244 to 746,900
uvrC
Position (kb) 744
745
746
747 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 744.502 kb on + strand at 744.524 kb on + strand at 744.651 kb on + strand, within uvrC at 744.856 kb on - strand, within uvrC at 744.877 kb on - strand, within uvrC at 744.877 kb on - strand, within uvrC at 744.895 kb on - strand, within uvrC at 744.963 kb on + strand, within uvrC at 744.964 kb on - strand, within uvrC at 744.965 kb on + strand, within uvrC at 745.041 kb on + strand, within uvrC at 745.107 kb on + strand, within uvrC at 745.107 kb on + strand, within uvrC at 745.108 kb on - strand, within uvrC at 745.224 kb on + strand, within uvrC at 745.225 kb on - strand, within uvrC at 745.273 kb on - strand, within uvrC at 745.278 kb on + strand, within uvrC at 745.279 kb on - strand, within uvrC at 745.337 kb on + strand, within uvrC at 745.348 kb on + strand, within uvrC at 745.667 kb on + strand, within uvrC at 745.668 kb on - strand, within uvrC at 745.668 kb on - strand, within uvrC at 745.735 kb on + strand, within uvrC at 745.743 kb on - strand, within uvrC at 745.842 kb on - strand, within uvrC at 745.936 kb on - strand, within uvrC at 745.973 kb on + strand, within uvrC at 745.997 kb on + strand, within uvrC at 745.998 kb on - strand, within uvrC at 746.044 kb on + strand, within uvrC at 746.103 kb on + strand at 746.111 kb on + strand at 746.153 kb on + strand at 746.154 kb on - strand at 746.154 kb on - strand at 746.154 kb on - strand at 746.154 kb on - strand at 746.154 kb on - strand at 746.158 kb on - strand at 746.178 kb on - strand at 746.216 kb on - strand at 746.257 kb on - strand at 746.331 kb on - strand, within uvrC at 746.340 kb on - strand, within uvrC at 746.430 kb on - strand, within uvrC at 746.430 kb on - strand, within uvrC at 746.531 kb on - strand, within uvrC at 746.531 kb on - strand, within uvrC at 746.531 kb on - strand, within uvrC at 746.531 kb on - strand, within uvrC at 746.746 kb on - strand, within uvrC at 746.820 kb on + strand, within uvrC at 746.821 kb on - strand, within uvrC at 746.821 kb on - strand, within uvrC at 746.830 kb on - strand, within uvrC at 746.830 kb on - strand, within uvrC at 746.830 kb on - strand, within uvrC at 746.830 kb on - strand, within uvrC at 746.832 kb on - strand, within uvrC at 746.887 kb on - strand at 746.887 kb on - strand at 746.887 kb on - strand at 746.907 kb on - strand at 746.931 kb on - strand at 746.937 kb on - strand at 746.937 kb on - strand at 746.950 kb on + strand at 746.951 kb on - strand at 746.951 kb on - strand at 746.951 kb on - strand at 747.041 kb on + strand at 747.042 kb on - strand at 747.042 kb on - strand at 747.121 kb on - strand at 747.156 kb on + strand at 747.162 kb on + strand at 747.163 kb on - strand at 747.178 kb on + strand at 747.207 kb on - strand at 747.220 kb on + strand at 747.220 kb on + strand at 747.221 kb on - strand at 747.221 kb on - strand at 747.222 kb on + strand at 747.223 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP9 remove 744,502 + -0.0 744,524 + -0.3 744,651 + uvrC MCAODC_19565 0.13 +1.3 744,856 - uvrC MCAODC_19565 0.24 +0.3 744,877 - uvrC MCAODC_19565 0.25 -0.7 744,877 - uvrC MCAODC_19565 0.25 +0.9 744,895 - uvrC MCAODC_19565 0.26 -0.2 744,963 + uvrC MCAODC_19565 0.30 -2.5 744,964 - uvrC MCAODC_19565 0.30 -2.5 744,965 + uvrC MCAODC_19565 0.30 +0.3 745,041 + uvrC MCAODC_19565 0.34 -2.5 745,107 + uvrC MCAODC_19565 0.38 -0.2 745,107 + uvrC MCAODC_19565 0.38 -1.7 745,108 - uvrC MCAODC_19565 0.38 -1.2 745,224 + uvrC MCAODC_19565 0.44 +0.2 745,225 - uvrC MCAODC_19565 0.44 +0.1 745,273 - uvrC MCAODC_19565 0.47 -1.5 745,278 + uvrC MCAODC_19565 0.47 -0.3 745,279 - uvrC MCAODC_19565 0.47 +0.3 745,337 + uvrC MCAODC_19565 0.50 +0.2 745,348 + uvrC MCAODC_19565 0.51 -1.1 745,667 + uvrC MCAODC_19565 0.68 -0.6 745,668 - uvrC MCAODC_19565 0.68 -1.8 745,668 - uvrC MCAODC_19565 0.68 -1.4 745,735 + uvrC MCAODC_19565 0.72 -0.5 745,743 - uvrC MCAODC_19565 0.72 +1.5 745,842 - uvrC MCAODC_19565 0.78 +0.3 745,936 - uvrC MCAODC_19565 0.83 -0.6 745,973 + uvrC MCAODC_19565 0.85 +1.3 745,997 + uvrC MCAODC_19565 0.86 -0.5 745,998 - uvrC MCAODC_19565 0.86 +1.1 746,044 + uvrC MCAODC_19565 0.89 -0.8 746,103 + -0.4 746,111 + +0.7 746,153 + +0.5 746,154 - -1.4 746,154 - -1.5 746,154 - +0.5 746,154 - -2.9 746,154 - -1.2 746,158 - +0.3 746,178 - -3.4 746,216 - -0.1 746,257 - -0.3 746,331 - uvrC MCAODC_19570 0.13 +1.2 746,340 - uvrC MCAODC_19570 0.15 -0.5 746,430 - uvrC MCAODC_19570 0.28 -0.5 746,430 - uvrC MCAODC_19570 0.28 +2.0 746,531 - uvrC MCAODC_19570 0.44 +2.0 746,531 - uvrC MCAODC_19570 0.44 +2.2 746,531 - uvrC MCAODC_19570 0.44 +1.0 746,531 - uvrC MCAODC_19570 0.44 -1.5 746,746 - uvrC MCAODC_19570 0.76 -1.5 746,820 + uvrC MCAODC_19570 0.88 +1.0 746,821 - uvrC MCAODC_19570 0.88 +0.8 746,821 - uvrC MCAODC_19570 0.88 +0.3 746,830 - uvrC MCAODC_19570 0.89 +0.7 746,830 - uvrC MCAODC_19570 0.89 +0.3 746,830 - uvrC MCAODC_19570 0.89 -0.3 746,830 - uvrC MCAODC_19570 0.89 +0.7 746,832 - uvrC MCAODC_19570 0.89 +1.3 746,887 - +1.5 746,887 - +0.6 746,887 - +0.5 746,907 - +1.5 746,931 - -0.4 746,937 - +0.1 746,937 - +0.3 746,950 + -1.8 746,951 - -0.0 746,951 - -1.4 746,951 - -1.5 747,041 + -2.6 747,042 - +0.8 747,042 - +1.0 747,121 - +1.6 747,156 + -0.5 747,162 + +1.4 747,163 - -0.1 747,178 + -2.3 747,207 - +1.3 747,220 + -0.4 747,220 + -0.1 747,221 - -1.5 747,221 - -0.5 747,222 + -1.8 747,223 - -2.0
Or see this region's nucleotide sequence