Strain Fitness in Escherichia coli ECRC101 around MCAODC_17035

Experiment: WM_TP9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_17020 and MCAODC_17025 overlap by 4 nucleotidesMCAODC_17025 and MCAODC_17030 overlap by 4 nucleotidesMCAODC_17030 and MCAODC_17035 are separated by 9 nucleotidesMCAODC_17035 and MCAODC_17040 overlap by 4 nucleotidesMCAODC_17040 and beeE overlap by 4 nucleotides MCAODC_17020: MCAODC_17020 - DUF3168 domain-containing protein, at 312,256 to 312,600 _17020 MCAODC_17025: MCAODC_17025 - phage portal protein, at 312,597 to 313,043 _17025 MCAODC_17030: MCAODC_17030 - phage head closure protein, at 313,040 to 313,390 _17030 MCAODC_17035: MCAODC_17035 - head-tail connector protein, at 313,400 to 313,726 _17035 MCAODC_17040: MCAODC_17040 - Portal protein, at 313,723 to 314,460 _17040 MCAODC_17045: beeE - phage portal protein, at 314,457 to 315,821 beeE Position (kb) 313 314Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 313.612 kb on - strand, within MCAODC_17035at 313.797 kb on + strand, within MCAODC_17040at 313.810 kb on - strand, within MCAODC_17040at 314.217 kb on - strand, within MCAODC_17040at 314.244 kb on + strand, within MCAODC_17040at 314.318 kb on - strand, within MCAODC_17040at 314.386 kb on - strand, within MCAODC_17040at 314.386 kb on - strand, within MCAODC_17040at 314.392 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_TP9
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313,612 - MCAODC_17035 0.65 +1.0
313,797 + MCAODC_17040 0.10 +0.3
313,810 - MCAODC_17040 0.12 +0.7
314,217 - MCAODC_17040 0.67 +0.5
314,244 + MCAODC_17040 0.71 -1.1
314,318 - MCAODC_17040 0.81 +0.7
314,386 - MCAODC_17040 0.90 -0.0
314,386 - MCAODC_17040 0.90 -4.6
314,392 + +1.6

Or see this region's nucleotide sequence