Experiment: WM_TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ortT and ydcY are separated by 85 nucleotides ydcY and MCAODC_16400 are separated by 0 nucleotides MCAODC_16400 and mddA overlap by 4 nucleotides mddA and curA are separated by 180 nucleotides
MCAODC_16390: ortT - orphan toxin OrtT, at 170,716 to 170,889
ortT
MCAODC_16395: ydcY - Uncharacterized protein YdcY, at 170,975 to 171,208
ydcY
MCAODC_16400: MCAODC_16400 - Putative inner membrane exporter, YdcZ, at 171,209 to 171,658
_16400
MCAODC_16405: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 171,655 to 172,173
mddA
MCAODC_16410: curA - NADPH-dependent curcumin/dihydrocurcumin reductase, at 172,354 to 173,391
curA
Position (kb) 171
172
173 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 170.927 kb on + strand at 171.051 kb on + strand, within ydcY at 171.098 kb on + strand, within ydcY at 171.151 kb on + strand, within ydcY at 171.151 kb on + strand, within ydcY at 171.151 kb on + strand, within ydcY at 171.151 kb on + strand, within ydcY at 171.152 kb on - strand, within ydcY at 171.361 kb on + strand, within MCAODC_16400 at 171.362 kb on - strand, within MCAODC_16400 at 171.435 kb on - strand, within MCAODC_16400 at 171.437 kb on - strand, within MCAODC_16400 at 171.437 kb on - strand, within MCAODC_16400 at 171.437 kb on - strand, within MCAODC_16400 at 171.485 kb on - strand, within MCAODC_16400 at 171.703 kb on - strand at 172.009 kb on + strand, within mddA at 172.031 kb on - strand, within mddA at 172.102 kb on - strand, within mddA at 172.103 kb on + strand, within mddA at 172.103 kb on + strand, within mddA at 172.192 kb on - strand at 172.192 kb on - strand at 172.192 kb on - strand at 172.212 kb on + strand at 172.212 kb on + strand at 172.318 kb on - strand at 172.479 kb on - strand, within curA at 172.510 kb on + strand, within curA at 172.510 kb on + strand, within curA at 172.511 kb on - strand, within curA at 172.512 kb on + strand, within curA at 172.512 kb on + strand, within curA at 172.512 kb on + strand, within curA at 172.524 kb on + strand, within curA at 172.524 kb on + strand, within curA at 172.524 kb on + strand, within curA at 172.543 kb on + strand, within curA at 172.544 kb on - strand, within curA at 172.673 kb on + strand, within curA at 172.767 kb on + strand, within curA at 172.767 kb on + strand, within curA at 172.767 kb on + strand, within curA at 172.775 kb on + strand, within curA at 172.775 kb on + strand, within curA at 172.775 kb on + strand, within curA at 172.794 kb on + strand, within curA at 172.818 kb on - strand, within curA at 173.013 kb on + strand, within curA at 173.017 kb on + strand, within curA at 173.038 kb on - strand, within curA at 173.070 kb on + strand, within curA at 173.110 kb on + strand, within curA at 173.111 kb on - strand, within curA at 173.113 kb on - strand, within curA at 173.126 kb on + strand, within curA at 173.147 kb on + strand, within curA at 173.163 kb on + strand, within curA at 173.163 kb on + strand, within curA
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP9 remove 170,927 + -1.6 171,051 + ydcY MCAODC_16395 0.32 +0.2 171,098 + ydcY MCAODC_16395 0.53 +1.6 171,151 + ydcY MCAODC_16395 0.75 -2.9 171,151 + ydcY MCAODC_16395 0.75 -1.0 171,151 + ydcY MCAODC_16395 0.75 -1.6 171,151 + ydcY MCAODC_16395 0.75 -0.3 171,152 - ydcY MCAODC_16395 0.76 -0.8 171,361 + MCAODC_16400 0.34 +4.2 171,362 - MCAODC_16400 0.34 -1.2 171,435 - MCAODC_16400 0.50 +0.7 171,437 - MCAODC_16400 0.51 +0.0 171,437 - MCAODC_16400 0.51 -0.5 171,437 - MCAODC_16400 0.51 -1.2 171,485 - MCAODC_16400 0.61 -0.7 171,703 - +0.2 172,009 + mddA MCAODC_16405 0.68 +0.5 172,031 - mddA MCAODC_16405 0.72 -0.7 172,102 - mddA MCAODC_16405 0.86 +1.2 172,103 + mddA MCAODC_16405 0.86 -0.3 172,103 + mddA MCAODC_16405 0.86 -0.8 172,192 - -1.7 172,192 - -0.0 172,192 - +0.9 172,212 + -1.4 172,212 + +0.1 172,318 - +1.1 172,479 - curA MCAODC_16410 0.12 +1.7 172,510 + curA MCAODC_16410 0.15 +0.3 172,510 + curA MCAODC_16410 0.15 +0.2 172,511 - curA MCAODC_16410 0.15 -1.0 172,512 + curA MCAODC_16410 0.15 -1.8 172,512 + curA MCAODC_16410 0.15 -0.1 172,512 + curA MCAODC_16410 0.15 -3.1 172,524 + curA MCAODC_16410 0.16 -0.3 172,524 + curA MCAODC_16410 0.16 +0.6 172,524 + curA MCAODC_16410 0.16 +1.8 172,543 + curA MCAODC_16410 0.18 +0.2 172,544 - curA MCAODC_16410 0.18 -0.0 172,673 + curA MCAODC_16410 0.31 +0.3 172,767 + curA MCAODC_16410 0.40 -0.7 172,767 + curA MCAODC_16410 0.40 +0.1 172,767 + curA MCAODC_16410 0.40 -0.7 172,775 + curA MCAODC_16410 0.41 -1.6 172,775 + curA MCAODC_16410 0.41 +2.9 172,775 + curA MCAODC_16410 0.41 -0.6 172,794 + curA MCAODC_16410 0.42 -0.4 172,818 - curA MCAODC_16410 0.45 +1.7 173,013 + curA MCAODC_16410 0.63 -0.7 173,017 + curA MCAODC_16410 0.64 +0.3 173,038 - curA MCAODC_16410 0.66 -2.0 173,070 + curA MCAODC_16410 0.69 -0.7 173,110 + curA MCAODC_16410 0.73 +0.6 173,111 - curA MCAODC_16410 0.73 +0.2 173,113 - curA MCAODC_16410 0.73 +3.2 173,126 + curA MCAODC_16410 0.74 -3.1 173,147 + curA MCAODC_16410 0.76 +1.2 173,163 + curA MCAODC_16410 0.78 +0.9 173,163 + curA MCAODC_16410 0.78 +1.6
Or see this region's nucleotide sequence