Experiment: WM_TP9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt serA and MCAODC_09990 are separated by 228 nucleotides MCAODC_09990 and MCAODC_10000 are separated by 333 nucleotides MCAODC_10000 and ygfA are separated by 100 nucleotides
MCAODC_09980: serA - phosphoglycerate dehydrogenase, at 1,988,865 to 1,990,097
serA
MCAODC_09990: MCAODC_09990 - Type I toxin-antitoxin system Ibs family toxin, at 1,990,326 to 1,990,385
_09990
MCAODC_10000: MCAODC_10000 - Type I toxin-antitoxin system Ibs family toxin, at 1,990,719 to 1,990,778
_10000
MCAODC_10005: ygfA - 5-formyltetrahydrofolate cyclo-ligase, at 1,990,879 to 1,991,481
ygfA
Position (kb)
1990
1991 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1989.347 kb on + strand, within serA at 1989.347 kb on + strand, within serA at 1989.348 kb on - strand, within serA at 1989.348 kb on - strand, within serA at 1989.390 kb on + strand, within serA at 1989.390 kb on + strand, within serA at 1989.390 kb on + strand, within serA at 1989.390 kb on + strand, within serA at 1989.390 kb on + strand, within serA at 1989.391 kb on - strand, within serA at 1989.391 kb on - strand, within serA at 1989.391 kb on - strand, within serA at 1989.391 kb on - strand, within serA at 1989.391 kb on - strand, within serA at 1989.391 kb on - strand, within serA at 1989.391 kb on - strand, within serA at 1989.391 kb on - strand, within serA at 1989.396 kb on + strand, within serA at 1989.396 kb on + strand, within serA at 1989.402 kb on + strand, within serA at 1989.402 kb on + strand, within serA at 1989.402 kb on + strand, within serA at 1989.402 kb on + strand, within serA at 1989.402 kb on + strand, within serA at 1989.445 kb on + strand, within serA at 1989.446 kb on - strand, within serA at 1989.521 kb on + strand, within serA at 1989.521 kb on + strand, within serA at 1989.599 kb on + strand, within serA at 1989.658 kb on + strand, within serA at 1989.659 kb on - strand, within serA at 1989.684 kb on - strand, within serA at 1989.695 kb on + strand, within serA at 1989.696 kb on - strand, within serA at 1989.696 kb on - strand, within serA at 1989.696 kb on - strand, within serA at 1989.917 kb on - strand, within serA at 1989.969 kb on + strand, within serA at 1989.973 kb on + strand, within serA at 1989.973 kb on + strand, within serA at 1989.974 kb on - strand at 1989.974 kb on - strand at 1989.996 kb on + strand at 1989.997 kb on - strand at 1990.000 kb on + strand at 1990.000 kb on + strand at 1990.000 kb on + strand at 1990.000 kb on + strand at 1990.001 kb on - strand at 1990.001 kb on - strand at 1990.001 kb on - strand at 1990.001 kb on - strand at 1990.004 kb on + strand at 1990.004 kb on + strand at 1990.005 kb on - strand at 1990.005 kb on - strand at 1990.011 kb on - strand at 1990.061 kb on + strand at 1990.092 kb on + strand at 1990.095 kb on + strand at 1990.096 kb on - strand at 1990.244 kb on - strand at 1990.354 kb on + strand, within MCAODC_09990 at 1990.366 kb on + strand, within MCAODC_09990 at 1990.384 kb on - strand at 1990.424 kb on + strand at 1990.527 kb on - strand at 1990.535 kb on - strand at 1990.538 kb on + strand at 1990.538 kb on + strand at 1990.539 kb on - strand at 1990.539 kb on - strand at 1990.546 kb on + strand at 1990.559 kb on + strand at 1990.559 kb on + strand at 1990.559 kb on + strand at 1990.559 kb on + strand at 1990.559 kb on + strand at 1990.559 kb on + strand at 1990.559 kb on + strand at 1990.559 kb on + strand at 1990.559 kb on + strand at 1990.560 kb on - strand at 1990.560 kb on - strand at 1990.560 kb on - strand at 1990.560 kb on - strand at 1990.560 kb on - strand at 1990.560 kb on - strand at 1990.560 kb on - strand at 1990.560 kb on - strand at 1990.560 kb on - strand at 1990.560 kb on - strand at 1990.560 kb on - strand at 1990.570 kb on + strand at 1990.570 kb on + strand at 1990.614 kb on - strand at 1990.637 kb on - strand at 1990.719 kb on - strand at 1990.738 kb on + strand, within MCAODC_10000 at 1990.738 kb on + strand, within MCAODC_10000 at 1990.738 kb on + strand, within MCAODC_10000 at 1990.747 kb on + strand, within MCAODC_10000 at 1990.753 kb on + strand, within MCAODC_10000 at 1990.774 kb on - strand at 1990.777 kb on - strand at 1990.905 kb on + strand at 1990.905 kb on + strand at 1990.905 kb on + strand at 1990.906 kb on - strand at 1990.996 kb on - strand, within ygfA at 1990.997 kb on + strand, within ygfA at 1991.014 kb on + strand, within ygfA at 1991.028 kb on - strand, within ygfA at 1991.028 kb on - strand, within ygfA at 1991.060 kb on + strand, within ygfA at 1991.061 kb on - strand, within ygfA at 1991.061 kb on - strand, within ygfA at 1991.206 kb on - strand, within ygfA at 1991.215 kb on - strand, within ygfA at 1991.237 kb on + strand, within ygfA at 1991.237 kb on + strand, within ygfA at 1991.239 kb on + strand, within ygfA at 1991.240 kb on - strand, within ygfA at 1991.269 kb on + strand, within ygfA at 1991.269 kb on + strand, within ygfA at 1991.270 kb on - strand, within ygfA at 1991.270 kb on - strand, within ygfA at 1991.271 kb on + strand, within ygfA at 1991.311 kb on + strand, within ygfA at 1991.312 kb on - strand, within ygfA at 1991.312 kb on - strand, within ygfA at 1991.345 kb on + strand, within ygfA at 1991.346 kb on - strand, within ygfA
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP9 remove 1,989,347 + serA MCAODC_09980 0.39 -4.0 1,989,347 + serA MCAODC_09980 0.39 -4.2 1,989,348 - serA MCAODC_09980 0.39 -2.5 1,989,348 - serA MCAODC_09980 0.39 -3.7 1,989,390 + serA MCAODC_09980 0.43 -5.3 1,989,390 + serA MCAODC_09980 0.43 -3.6 1,989,390 + serA MCAODC_09980 0.43 -4.4 1,989,390 + serA MCAODC_09980 0.43 -3.7 1,989,390 + serA MCAODC_09980 0.43 -4.2 1,989,391 - serA MCAODC_09980 0.43 -3.9 1,989,391 - serA MCAODC_09980 0.43 -4.1 1,989,391 - serA MCAODC_09980 0.43 -4.4 1,989,391 - serA MCAODC_09980 0.43 -3.2 1,989,391 - serA MCAODC_09980 0.43 -0.6 1,989,391 - serA MCAODC_09980 0.43 -4.3 1,989,391 - serA MCAODC_09980 0.43 -2.8 1,989,391 - serA MCAODC_09980 0.43 -2.1 1,989,396 + serA MCAODC_09980 0.43 -2.6 1,989,396 + serA MCAODC_09980 0.43 -2.2 1,989,402 + serA MCAODC_09980 0.44 -2.2 1,989,402 + serA MCAODC_09980 0.44 -3.5 1,989,402 + serA MCAODC_09980 0.44 -2.9 1,989,402 + serA MCAODC_09980 0.44 -3.0 1,989,402 + serA MCAODC_09980 0.44 -5.3 1,989,445 + serA MCAODC_09980 0.47 -4.4 1,989,446 - serA MCAODC_09980 0.47 -4.1 1,989,521 + serA MCAODC_09980 0.53 -2.3 1,989,521 + serA MCAODC_09980 0.53 -4.3 1,989,599 + serA MCAODC_09980 0.60 -2.1 1,989,658 + serA MCAODC_09980 0.64 -3.6 1,989,659 - serA MCAODC_09980 0.64 -3.4 1,989,684 - serA MCAODC_09980 0.66 -1.6 1,989,695 + serA MCAODC_09980 0.67 -3.9 1,989,696 - serA MCAODC_09980 0.67 -3.3 1,989,696 - serA MCAODC_09980 0.67 -2.6 1,989,696 - serA MCAODC_09980 0.67 -3.7 1,989,917 - serA MCAODC_09980 0.85 -3.2 1,989,969 + serA MCAODC_09980 0.90 -3.8 1,989,973 + serA MCAODC_09980 0.90 -2.3 1,989,973 + serA MCAODC_09980 0.90 -3.6 1,989,974 - -3.3 1,989,974 - -4.2 1,989,996 + -4.8 1,989,997 - -3.4 1,990,000 + -1.8 1,990,000 + -3.7 1,990,000 + -2.5 1,990,000 + -1.2 1,990,001 - -3.9 1,990,001 - -4.0 1,990,001 - -4.5 1,990,001 - -3.9 1,990,004 + -4.5 1,990,004 + -3.3 1,990,005 - -3.2 1,990,005 - -3.0 1,990,011 - -0.6 1,990,061 + -4.4 1,990,092 + +0.1 1,990,095 + -0.1 1,990,096 - -1.7 1,990,244 - +0.0 1,990,354 + MCAODC_09990 0.47 +0.6 1,990,366 + MCAODC_09990 0.67 +0.5 1,990,384 - -3.7 1,990,424 + +1.2 1,990,527 - -0.3 1,990,535 - +0.5 1,990,538 + -2.4 1,990,538 + -0.1 1,990,539 - -1.9 1,990,539 - -0.8 1,990,546 + +0.4 1,990,559 + +0.2 1,990,559 + +0.7 1,990,559 + +1.0 1,990,559 + -0.2 1,990,559 + +0.4 1,990,559 + -0.2 1,990,559 + +0.4 1,990,559 + -0.7 1,990,559 + -1.1 1,990,560 - +0.3 1,990,560 - +0.3 1,990,560 - -0.2 1,990,560 - +0.4 1,990,560 - +0.1 1,990,560 - -0.4 1,990,560 - -1.6 1,990,560 - -0.6 1,990,560 - -2.4 1,990,560 - +0.8 1,990,560 - +0.3 1,990,570 + +0.0 1,990,570 + +0.8 1,990,614 - -0.2 1,990,637 - +1.5 1,990,719 - +0.4 1,990,738 + MCAODC_10000 0.32 +0.1 1,990,738 + MCAODC_10000 0.32 -2.2 1,990,738 + MCAODC_10000 0.32 -0.9 1,990,747 + MCAODC_10000 0.47 +0.8 1,990,753 + MCAODC_10000 0.57 -0.2 1,990,774 - +0.1 1,990,777 - -0.2 1,990,905 + +2.1 1,990,905 + +2.1 1,990,905 + -0.8 1,990,906 - -2.0 1,990,996 - ygfA MCAODC_10005 0.19 +0.5 1,990,997 + ygfA MCAODC_10005 0.20 -0.9 1,991,014 + ygfA MCAODC_10005 0.22 +0.1 1,991,028 - ygfA MCAODC_10005 0.25 +0.4 1,991,028 - ygfA MCAODC_10005 0.25 +1.5 1,991,060 + ygfA MCAODC_10005 0.30 -0.1 1,991,061 - ygfA MCAODC_10005 0.30 -2.2 1,991,061 - ygfA MCAODC_10005 0.30 +0.2 1,991,206 - ygfA MCAODC_10005 0.54 +0.5 1,991,215 - ygfA MCAODC_10005 0.56 +0.7 1,991,237 + ygfA MCAODC_10005 0.59 +0.0 1,991,237 + ygfA MCAODC_10005 0.59 +2.9 1,991,239 + ygfA MCAODC_10005 0.60 +0.3 1,991,240 - ygfA MCAODC_10005 0.60 +1.2 1,991,269 + ygfA MCAODC_10005 0.65 +1.3 1,991,269 + ygfA MCAODC_10005 0.65 -0.3 1,991,270 - ygfA MCAODC_10005 0.65 +0.5 1,991,270 - ygfA MCAODC_10005 0.65 -0.1 1,991,271 + ygfA MCAODC_10005 0.65 +1.4 1,991,311 + ygfA MCAODC_10005 0.72 +0.7 1,991,312 - ygfA MCAODC_10005 0.72 +0.1 1,991,312 - ygfA MCAODC_10005 0.72 +0.1 1,991,345 + ygfA MCAODC_10005 0.77 +0.5 1,991,346 - ygfA MCAODC_10005 0.77 +0.1
Or see this region's nucleotide sequence