Experiment: WM_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rutD and rutE are separated by 9 nucleotides rutE and rutF are separated by 10 nucleotides rutF and rutG are separated by 20 nucleotides
MCAODC_24015: rutD - pyrimidine utilization protein D, at 1,530,470 to 1,531,270
rutD
MCAODC_24020: rutE - malonic semialdehyde reductase, at 1,531,280 to 1,531,870
rutE
MCAODC_24025: rutF - pyrimidine utilization flavin reductase protein F, at 1,531,881 to 1,532,375
rutF
MCAODC_24030: rutG - pyrimidine utilization transport protein G, at 1,532,396 to 1,533,724
rutG
Position (kb) 1531
1532
1533 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1531.003 kb on - strand, within rutD at 1531.016 kb on - strand, within rutD at 1531.211 kb on - strand at 1531.245 kb on + strand at 1531.245 kb on + strand at 1531.246 kb on - strand at 1531.246 kb on - strand at 1531.246 kb on - strand at 1531.246 kb on - strand at 1531.279 kb on + strand at 1531.280 kb on - strand at 1531.280 kb on - strand at 1531.421 kb on - strand, within rutE at 1531.421 kb on - strand, within rutE at 1531.421 kb on - strand, within rutE at 1531.421 kb on - strand, within rutE at 1531.421 kb on - strand, within rutE at 1531.438 kb on + strand, within rutE at 1531.438 kb on + strand, within rutE at 1531.740 kb on + strand, within rutE at 1531.741 kb on - strand, within rutE at 1531.789 kb on + strand, within rutE at 1531.789 kb on + strand, within rutE at 1531.792 kb on + strand, within rutE at 1531.868 kb on + strand at 1531.869 kb on - strand at 1531.881 kb on - strand at 1531.881 kb on - strand at 1531.881 kb on - strand at 1531.881 kb on - strand at 1532.092 kb on + strand, within rutF at 1532.093 kb on - strand, within rutF at 1532.096 kb on + strand, within rutF at 1532.096 kb on + strand, within rutF at 1532.346 kb on + strand at 1532.427 kb on + strand at 1532.497 kb on - strand at 1532.542 kb on - strand, within rutG at 1532.583 kb on + strand, within rutG at 1532.584 kb on - strand, within rutG at 1532.584 kb on - strand, within rutG at 1532.605 kb on - strand, within rutG at 1532.643 kb on - strand, within rutG at 1532.643 kb on - strand, within rutG at 1532.762 kb on + strand, within rutG at 1532.763 kb on - strand, within rutG at 1532.764 kb on + strand, within rutG at 1532.875 kb on + strand, within rutG at 1532.875 kb on + strand, within rutG at 1532.911 kb on + strand, within rutG at 1532.912 kb on - strand, within rutG at 1532.942 kb on - strand, within rutG at 1532.997 kb on + strand, within rutG at 1533.045 kb on + strand, within rutG at 1533.070 kb on + strand, within rutG at 1533.128 kb on - strand, within rutG at 1533.187 kb on - strand, within rutG at 1533.226 kb on + strand, within rutG at 1533.236 kb on + strand, within rutG at 1533.236 kb on + strand, within rutG at 1533.236 kb on + strand, within rutG at 1533.237 kb on - strand, within rutG at 1533.238 kb on + strand, within rutG at 1533.314 kb on + strand, within rutG at 1533.314 kb on + strand, within rutG at 1533.314 kb on + strand, within rutG at 1533.314 kb on + strand, within rutG at 1533.314 kb on + strand, within rutG at 1533.315 kb on - strand, within rutG
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas14 remove 1,531,003 - rutD MCAODC_24015 0.67 +0.4 1,531,016 - rutD MCAODC_24015 0.68 -1.6 1,531,211 - -0.1 1,531,245 + +0.7 1,531,245 + -0.2 1,531,246 - -0.4 1,531,246 - -0.4 1,531,246 - +0.6 1,531,246 - +0.1 1,531,279 + +1.4 1,531,280 - -0.8 1,531,280 - +0.0 1,531,421 - rutE MCAODC_24020 0.24 +0.7 1,531,421 - rutE MCAODC_24020 0.24 +0.4 1,531,421 - rutE MCAODC_24020 0.24 -0.3 1,531,421 - rutE MCAODC_24020 0.24 -0.2 1,531,421 - rutE MCAODC_24020 0.24 +0.6 1,531,438 + rutE MCAODC_24020 0.27 +0.6 1,531,438 + rutE MCAODC_24020 0.27 -2.3 1,531,740 + rutE MCAODC_24020 0.78 +0.4 1,531,741 - rutE MCAODC_24020 0.78 -1.2 1,531,789 + rutE MCAODC_24020 0.86 +1.0 1,531,789 + rutE MCAODC_24020 0.86 +2.7 1,531,792 + rutE MCAODC_24020 0.87 -0.6 1,531,868 + +1.1 1,531,869 - +0.3 1,531,881 - +0.4 1,531,881 - -0.8 1,531,881 - -1.0 1,531,881 - +0.8 1,532,092 + rutF MCAODC_24025 0.43 +1.4 1,532,093 - rutF MCAODC_24025 0.43 +0.1 1,532,096 + rutF MCAODC_24025 0.43 +0.9 1,532,096 + rutF MCAODC_24025 0.43 -1.1 1,532,346 + -0.7 1,532,427 + +0.7 1,532,497 - -0.1 1,532,542 - rutG MCAODC_24030 0.11 +0.7 1,532,583 + rutG MCAODC_24030 0.14 +0.1 1,532,584 - rutG MCAODC_24030 0.14 -0.8 1,532,584 - rutG MCAODC_24030 0.14 -0.6 1,532,605 - rutG MCAODC_24030 0.16 -1.8 1,532,643 - rutG MCAODC_24030 0.19 -0.3 1,532,643 - rutG MCAODC_24030 0.19 -0.9 1,532,762 + rutG MCAODC_24030 0.28 +0.2 1,532,763 - rutG MCAODC_24030 0.28 -0.5 1,532,764 + rutG MCAODC_24030 0.28 +2.4 1,532,875 + rutG MCAODC_24030 0.36 -0.8 1,532,875 + rutG MCAODC_24030 0.36 +0.2 1,532,911 + rutG MCAODC_24030 0.39 -0.1 1,532,912 - rutG MCAODC_24030 0.39 +0.5 1,532,942 - rutG MCAODC_24030 0.41 +0.5 1,532,997 + rutG MCAODC_24030 0.45 -0.1 1,533,045 + rutG MCAODC_24030 0.49 +0.5 1,533,070 + rutG MCAODC_24030 0.51 +0.8 1,533,128 - rutG MCAODC_24030 0.55 +0.4 1,533,187 - rutG MCAODC_24030 0.60 +0.3 1,533,226 + rutG MCAODC_24030 0.62 -0.9 1,533,236 + rutG MCAODC_24030 0.63 -0.8 1,533,236 + rutG MCAODC_24030 0.63 +0.3 1,533,236 + rutG MCAODC_24030 0.63 +0.6 1,533,237 - rutG MCAODC_24030 0.63 -0.5 1,533,238 + rutG MCAODC_24030 0.63 -0.0 1,533,314 + rutG MCAODC_24030 0.69 +0.6 1,533,314 + rutG MCAODC_24030 0.69 -1.0 1,533,314 + rutG MCAODC_24030 0.69 -1.4 1,533,314 + rutG MCAODC_24030 0.69 +1.3 1,533,314 + rutG MCAODC_24030 0.69 +0.2 1,533,315 - rutG MCAODC_24030 0.69 -1.8
Or see this region's nucleotide sequence