Strain Fitness in Escherichia coli ECRC101 around MCAODC_22460

Experiment: WM_Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpepT and potA are separated by 249 nucleotidespotA and potB overlap by 17 nucleotides MCAODC_22455: pepT - peptidase T, at 1,249,814 to 1,251,040 pepT MCAODC_22460: potA - spermidine/putrescine ABC transporter ATP-binding protein PotA, at 1,251,290 to 1,252,426 potA MCAODC_22465: potB - spermidine/putrescine ABC transporter permease PotB, at 1,252,410 to 1,253,273 potB Position (kb) 1251 1252 1253Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1250.321 kb on - strand, within pepTat 1250.464 kb on - strand, within pepTat 1250.631 kb on - strand, within pepTat 1250.641 kb on + strand, within pepTat 1250.642 kb on - strand, within pepTat 1250.696 kb on - strand, within pepTat 1250.735 kb on - strand, within pepTat 1250.939 kb on + strandat 1251.042 kb on + strandat 1251.071 kb on - strandat 1251.073 kb on + strandat 1251.094 kb on + strandat 1251.131 kb on + strandat 1251.132 kb on - strandat 1251.132 kb on - strandat 1251.136 kb on - strandat 1251.144 kb on + strandat 1251.165 kb on - strandat 1251.217 kb on + strandat 1251.217 kb on + strandat 1251.229 kb on + strandat 1251.229 kb on + strandat 1251.270 kb on - strandat 1251.581 kb on + strand, within potAat 1251.581 kb on + strand, within potAat 1251.886 kb on + strand, within potAat 1251.887 kb on - strand, within potAat 1252.045 kb on + strand, within potAat 1252.117 kb on + strand, within potAat 1252.117 kb on + strand, within potAat 1252.120 kb on + strand, within potAat 1252.120 kb on + strand, within potAat 1252.124 kb on + strand, within potAat 1252.125 kb on - strand, within potAat 1252.125 kb on - strand, within potAat 1252.185 kb on + strand, within potAat 1252.186 kb on - strand, within potAat 1252.235 kb on + strand, within potAat 1252.250 kb on - strand, within potAat 1252.254 kb on - strand, within potAat 1252.375 kb on + strandat 1252.424 kb on + strandat 1252.438 kb on + strandat 1252.566 kb on + strand, within potBat 1252.603 kb on + strand, within potBat 1252.941 kb on - strand, within potBat 1252.994 kb on + strand, within potBat 1253.076 kb on + strand, within potBat 1253.076 kb on + strand, within potBat 1253.077 kb on - strand, within potBat 1253.077 kb on - strand, within potBat 1253.077 kb on - strand, within potBat 1253.134 kb on + strand, within potBat 1253.134 kb on + strand, within potBat 1253.147 kb on + strand, within potBat 1253.184 kb on - strand, within potBat 1253.349 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas14
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1,250,321 - pepT MCAODC_22455 0.41 +0.4
1,250,464 - pepT MCAODC_22455 0.53 -0.6
1,250,631 - pepT MCAODC_22455 0.67 -0.5
1,250,641 + pepT MCAODC_22455 0.67 -0.3
1,250,642 - pepT MCAODC_22455 0.67 +0.2
1,250,696 - pepT MCAODC_22455 0.72 -0.5
1,250,735 - pepT MCAODC_22455 0.75 -0.4
1,250,939 + +1.1
1,251,042 + -1.9
1,251,071 - +1.2
1,251,073 + +0.5
1,251,094 + +0.0
1,251,131 + +0.6
1,251,132 - -1.3
1,251,132 - +0.1
1,251,136 - -1.7
1,251,144 + +0.3
1,251,165 - +0.4
1,251,217 + +2.3
1,251,217 + +0.3
1,251,229 + -0.8
1,251,229 + +0.5
1,251,270 - +0.1
1,251,581 + potA MCAODC_22460 0.26 +1.2
1,251,581 + potA MCAODC_22460 0.26 -0.2
1,251,886 + potA MCAODC_22460 0.52 +0.2
1,251,887 - potA MCAODC_22460 0.53 +1.1
1,252,045 + potA MCAODC_22460 0.66 +1.2
1,252,117 + potA MCAODC_22460 0.73 +1.1
1,252,117 + potA MCAODC_22460 0.73 +2.3
1,252,120 + potA MCAODC_22460 0.73 +0.9
1,252,120 + potA MCAODC_22460 0.73 +1.1
1,252,124 + potA MCAODC_22460 0.73 -0.3
1,252,125 - potA MCAODC_22460 0.73 -0.3
1,252,125 - potA MCAODC_22460 0.73 -2.1
1,252,185 + potA MCAODC_22460 0.79 +0.4
1,252,186 - potA MCAODC_22460 0.79 +0.7
1,252,235 + potA MCAODC_22460 0.83 -0.6
1,252,250 - potA MCAODC_22460 0.84 -1.0
1,252,254 - potA MCAODC_22460 0.85 -2.9
1,252,375 + -0.3
1,252,424 + +1.6
1,252,438 + +0.9
1,252,566 + potB MCAODC_22465 0.18 +0.5
1,252,603 + potB MCAODC_22465 0.22 +0.3
1,252,941 - potB MCAODC_22465 0.61 +0.8
1,252,994 + potB MCAODC_22465 0.68 +0.6
1,253,076 + potB MCAODC_22465 0.77 +0.7
1,253,076 + potB MCAODC_22465 0.77 -3.1
1,253,077 - potB MCAODC_22465 0.77 +0.3
1,253,077 - potB MCAODC_22465 0.77 -0.2
1,253,077 - potB MCAODC_22465 0.77 +1.2
1,253,134 + potB MCAODC_22465 0.84 +0.1
1,253,134 + potB MCAODC_22465 0.84 +1.1
1,253,147 + potB MCAODC_22465 0.85 -0.8
1,253,184 - potB MCAODC_22465 0.90 +0.3
1,253,349 - -0.4

Or see this region's nucleotide sequence