Strain Fitness in Escherichia coli ECRC101 around MCAODC_20220

Experiment: WM_Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycdB and Asn_trna are separated by 449 nucleotidesAsn_trna and yeeO are separated by 100 nucleotides MCAODC_20215: ycdB - putative transcriptional regulatory protein YcdB, at 855,536 to 856,252 ycdB MCAODC_20220: Asn_trna - tRNA-Asn(gtt), at 856,702 to 856,777 _trna MCAODC_20225: yeeO - toxic metabolite efflux MATE transporter YeeO, at 856,878 to 858,332 yeeO Position (kb) 856 857Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 856.042 kb on + strand, within ycdBat 856.046 kb on + strand, within ycdBat 856.345 kb on - strandat 856.679 kb on - strandat 856.769 kb on + strand, within Asn_trnaat 857.102 kb on + strand, within yeeOat 857.130 kb on - strand, within yeeOat 857.130 kb on - strand, within yeeOat 857.143 kb on - strand, within yeeOat 857.172 kb on - strand, within yeeOat 857.213 kb on + strand, within yeeOat 857.378 kb on + strand, within yeeOat 857.378 kb on + strand, within yeeOat 857.414 kb on + strand, within yeeOat 857.415 kb on - strand, within yeeOat 857.494 kb on - strand, within yeeOat 857.747 kb on + strand, within yeeOat 857.748 kb on - strand, within yeeOat 857.753 kb on + strand, within yeeOat 857.753 kb on + strand, within yeeO

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas14
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856,042 + ycdB MCAODC_20215 0.71 +1.8
856,046 + ycdB MCAODC_20215 0.71 -0.3
856,345 - +0.1
856,679 - -0.2
856,769 + Asn_trna MCAODC_20220 0.88 +0.2
857,102 + yeeO MCAODC_20225 0.15 +0.6
857,130 - yeeO MCAODC_20225 0.17 -1.9
857,130 - yeeO MCAODC_20225 0.17 -2.8
857,143 - yeeO MCAODC_20225 0.18 -0.8
857,172 - yeeO MCAODC_20225 0.20 -1.4
857,213 + yeeO MCAODC_20225 0.23 -0.9
857,378 + yeeO MCAODC_20225 0.34 +0.2
857,378 + yeeO MCAODC_20225 0.34 -0.5
857,414 + yeeO MCAODC_20225 0.37 +0.8
857,415 - yeeO MCAODC_20225 0.37 -1.8
857,494 - yeeO MCAODC_20225 0.42 -0.5
857,747 + yeeO MCAODC_20225 0.60 -1.0
857,748 - yeeO MCAODC_20225 0.60 +0.6
857,753 + yeeO MCAODC_20225 0.60 +0.4
857,753 + yeeO MCAODC_20225 0.60 -0.5

Or see this region's nucleotide sequence