Experiment: WM_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt xerD and Ser_trna are separated by 8 nucleotides Ser_trna and mtfA are separated by 93 nucleotides mtfA and Asn_trna are separated by 100 nucleotides Asn_trna and MCAODC_20190 are separated by 123 nucleotides MCAODC_20190 and MCAODC_20195 are separated by 13 nucleotides
MCAODC_20170: xerD - integrase, at 840,153 to 841,232
xerD
MCAODC_20175: Ser_trna - tRNA-Ser(cga), at 841,241 to 841,330
_trna
MCAODC_20180: mtfA - DgsA anti-repressor MtfA, at 841,424 to 842,221
mtfA
MCAODC_20185: Asn_trna - tRNA-Asn(gtt), at 842,322 to 842,397
_trna
MCAODC_20190: MCAODC_20190 - hypothetical protein, at 842,521 to 842,670
_20190
MCAODC_20195: MCAODC_20195 - Ig-like domain-containing protein, at 842,684 to 850,693
_20195
Position (kb) 841
842
843 Strain fitness (log2 ratio) -2
-1
0
1
2 at 840.467 kb on - strand, within xerD at 840.489 kb on + strand, within xerD at 840.489 kb on + strand, within xerD at 840.515 kb on + strand, within xerD at 840.560 kb on + strand, within xerD at 840.560 kb on + strand, within xerD at 840.560 kb on + strand, within xerD at 840.561 kb on - strand, within xerD at 840.611 kb on - strand, within xerD at 840.642 kb on + strand, within xerD at 840.646 kb on + strand, within xerD at 840.646 kb on + strand, within xerD at 840.646 kb on + strand, within xerD at 840.673 kb on + strand, within xerD at 840.718 kb on - strand, within xerD at 840.872 kb on - strand, within xerD at 840.889 kb on + strand, within xerD at 840.915 kb on + strand, within xerD at 840.915 kb on + strand, within xerD at 840.917 kb on + strand, within xerD at 840.917 kb on + strand, within xerD at 840.917 kb on + strand, within xerD at 840.917 kb on + strand, within xerD at 840.918 kb on - strand, within xerD at 840.918 kb on - strand, within xerD at 840.918 kb on - strand, within xerD at 840.918 kb on - strand, within xerD at 840.918 kb on - strand, within xerD at 840.918 kb on - strand, within xerD at 840.918 kb on - strand, within xerD at 840.921 kb on + strand, within xerD at 840.921 kb on + strand, within xerD at 840.921 kb on + strand, within xerD at 841.099 kb on - strand, within xerD at 841.099 kb on - strand, within xerD at 841.217 kb on - strand at 841.429 kb on + strand at 841.430 kb on - strand at 841.507 kb on - strand, within mtfA at 841.507 kb on - strand, within mtfA at 841.588 kb on - strand, within mtfA at 841.635 kb on + strand, within mtfA at 841.635 kb on + strand, within mtfA at 841.636 kb on - strand, within mtfA at 841.660 kb on - strand, within mtfA at 841.695 kb on + strand, within mtfA at 841.695 kb on + strand, within mtfA at 841.766 kb on - strand, within mtfA at 841.766 kb on - strand, within mtfA at 841.825 kb on - strand, within mtfA at 841.879 kb on + strand, within mtfA at 841.880 kb on - strand, within mtfA at 842.033 kb on + strand, within mtfA at 842.188 kb on + strand at 842.223 kb on + strand at 842.439 kb on + strand at 842.439 kb on + strand at 842.439 kb on + strand at 842.439 kb on + strand at 842.439 kb on + strand at 842.483 kb on + strand at 842.500 kb on + strand at 842.565 kb on - strand, within MCAODC_20190 at 842.635 kb on - strand, within MCAODC_20190 at 842.669 kb on - strand at 842.669 kb on - strand at 842.683 kb on + strand at 842.689 kb on - strand at 842.706 kb on + strand at 842.742 kb on + strand at 842.776 kb on + strand at 842.777 kb on - strand at 842.790 kb on + strand at 843.106 kb on + strand at 843.106 kb on + strand at 843.107 kb on - strand at 843.140 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas14 remove 840,467 - xerD MCAODC_20170 0.29 +0.7 840,489 + xerD MCAODC_20170 0.31 +0.0 840,489 + xerD MCAODC_20170 0.31 +2.1 840,515 + xerD MCAODC_20170 0.34 -0.8 840,560 + xerD MCAODC_20170 0.38 -0.0 840,560 + xerD MCAODC_20170 0.38 +1.4 840,560 + xerD MCAODC_20170 0.38 +0.0 840,561 - xerD MCAODC_20170 0.38 +0.4 840,611 - xerD MCAODC_20170 0.42 -0.2 840,642 + xerD MCAODC_20170 0.45 -1.5 840,646 + xerD MCAODC_20170 0.46 -0.0 840,646 + xerD MCAODC_20170 0.46 -1.8 840,646 + xerD MCAODC_20170 0.46 +1.8 840,673 + xerD MCAODC_20170 0.48 +0.2 840,718 - xerD MCAODC_20170 0.52 -0.3 840,872 - xerD MCAODC_20170 0.67 +1.1 840,889 + xerD MCAODC_20170 0.68 +0.5 840,915 + xerD MCAODC_20170 0.71 -1.2 840,915 + xerD MCAODC_20170 0.71 -0.0 840,917 + xerD MCAODC_20170 0.71 +1.2 840,917 + xerD MCAODC_20170 0.71 +0.8 840,917 + xerD MCAODC_20170 0.71 +0.8 840,917 + xerD MCAODC_20170 0.71 -0.7 840,918 - xerD MCAODC_20170 0.71 +0.1 840,918 - xerD MCAODC_20170 0.71 -1.2 840,918 - xerD MCAODC_20170 0.71 +0.1 840,918 - xerD MCAODC_20170 0.71 -1.2 840,918 - xerD MCAODC_20170 0.71 +0.2 840,918 - xerD MCAODC_20170 0.71 -1.0 840,918 - xerD MCAODC_20170 0.71 -0.4 840,921 + xerD MCAODC_20170 0.71 +0.7 840,921 + xerD MCAODC_20170 0.71 -0.6 840,921 + xerD MCAODC_20170 0.71 +1.0 841,099 - xerD MCAODC_20170 0.88 +0.1 841,099 - xerD MCAODC_20170 0.88 -0.8 841,217 - -0.1 841,429 + +1.3 841,430 - +0.0 841,507 - mtfA MCAODC_20180 0.10 -0.2 841,507 - mtfA MCAODC_20180 0.10 -0.6 841,588 - mtfA MCAODC_20180 0.21 +0.6 841,635 + mtfA MCAODC_20180 0.26 -0.5 841,635 + mtfA MCAODC_20180 0.26 +1.1 841,636 - mtfA MCAODC_20180 0.27 +0.6 841,660 - mtfA MCAODC_20180 0.30 -0.8 841,695 + mtfA MCAODC_20180 0.34 -0.7 841,695 + mtfA MCAODC_20180 0.34 +0.6 841,766 - mtfA MCAODC_20180 0.43 +0.0 841,766 - mtfA MCAODC_20180 0.43 +0.6 841,825 - mtfA MCAODC_20180 0.50 -0.1 841,879 + mtfA MCAODC_20180 0.57 +0.2 841,880 - mtfA MCAODC_20180 0.57 +1.3 842,033 + mtfA MCAODC_20180 0.76 +1.6 842,188 + +0.8 842,223 + -0.6 842,439 + -0.7 842,439 + +0.1 842,439 + -1.2 842,439 + +0.2 842,439 + +0.6 842,483 + -0.0 842,500 + +0.6 842,565 - MCAODC_20190 0.29 +0.1 842,635 - MCAODC_20190 0.76 +0.4 842,669 - +0.3 842,669 - -0.8 842,683 + +0.2 842,689 - -0.4 842,706 + -0.7 842,742 + -0.7 842,776 + -1.2 842,777 - +0.2 842,790 + -0.2 843,106 + -0.5 843,106 + -1.2 843,107 - +1.0 843,140 - -1.7
Or see this region's nucleotide sequence