Strain Fitness in Escherichia coli ECRC101 around MCAODC_16165

Experiment: WM_Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_16160 and MCAODC_16165 are separated by 219 nucleotidesMCAODC_16165 and pdxI are separated by 219 nucleotides MCAODC_16160: MCAODC_16160 - surface-exposed virulence protein BigA, at 121,682 to 124,717 _16160 MCAODC_16165: MCAODC_16165 - BigB-like protein, at 124,937 to 127,396 _16165 MCAODC_16170: pdxI - pyridoxine 4-dehydrogenase, at 127,616 to 128,476 pdxI Position (kb) 124 125 126 127 128Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 124.048 kb on + strand, within MCAODC_16160at 124.157 kb on - strand, within MCAODC_16160at 124.169 kb on + strand, within MCAODC_16160at 124.210 kb on + strand, within MCAODC_16160at 124.210 kb on + strand, within MCAODC_16160at 124.210 kb on + strand, within MCAODC_16160at 124.214 kb on + strand, within MCAODC_16160at 124.330 kb on + strand, within MCAODC_16160at 124.330 kb on + strand, within MCAODC_16160at 124.342 kb on + strand, within MCAODC_16160at 124.342 kb on + strand, within MCAODC_16160at 124.391 kb on + strand, within MCAODC_16160at 124.393 kb on + strand, within MCAODC_16160at 124.561 kb on + strandat 124.700 kb on - strandat 124.730 kb on + strandat 124.748 kb on + strandat 124.762 kb on - strandat 124.803 kb on + strandat 124.806 kb on + strandat 124.971 kb on + strandat 124.971 kb on + strandat 125.061 kb on + strandat 125.065 kb on + strandat 125.068 kb on + strandat 125.092 kb on - strandat 125.107 kb on + strandat 125.116 kb on + strandat 125.212 kb on + strand, within MCAODC_16165at 125.388 kb on + strand, within MCAODC_16165at 125.388 kb on + strand, within MCAODC_16165at 125.390 kb on + strand, within MCAODC_16165at 125.391 kb on - strand, within MCAODC_16165at 125.392 kb on + strand, within MCAODC_16165at 125.392 kb on + strand, within MCAODC_16165at 125.392 kb on + strand, within MCAODC_16165at 125.393 kb on - strand, within MCAODC_16165at 125.424 kb on + strand, within MCAODC_16165at 125.512 kb on + strand, within MCAODC_16165at 125.569 kb on + strand, within MCAODC_16165at 125.570 kb on - strand, within MCAODC_16165at 125.618 kb on + strand, within MCAODC_16165at 125.618 kb on + strand, within MCAODC_16165at 125.793 kb on + strand, within MCAODC_16165at 125.885 kb on - strand, within MCAODC_16165at 125.994 kb on - strand, within MCAODC_16165at 126.029 kb on + strand, within MCAODC_16165at 126.029 kb on + strand, within MCAODC_16165at 126.029 kb on + strand, within MCAODC_16165at 126.029 kb on + strand, within MCAODC_16165at 126.030 kb on - strand, within MCAODC_16165at 126.070 kb on + strand, within MCAODC_16165at 126.071 kb on - strand, within MCAODC_16165at 126.101 kb on + strand, within MCAODC_16165at 126.350 kb on + strand, within MCAODC_16165at 126.428 kb on + strand, within MCAODC_16165at 126.431 kb on - strand, within MCAODC_16165at 126.641 kb on + strand, within MCAODC_16165at 126.661 kb on + strand, within MCAODC_16165at 126.662 kb on - strand, within MCAODC_16165at 126.817 kb on + strand, within MCAODC_16165at 127.295 kb on + strandat 127.295 kb on + strandat 127.295 kb on + strandat 127.331 kb on + strandat 127.473 kb on - strandat 127.553 kb on - strandat 127.557 kb on - strandat 127.573 kb on - strandat 127.575 kb on - strandat 127.601 kb on - strandat 127.627 kb on + strandat 127.634 kb on - strandat 127.634 kb on - strandat 127.791 kb on - strand, within pdxIat 127.791 kb on - strand, within pdxIat 128.169 kb on - strand, within pdxIat 128.343 kb on + strand, within pdxIat 128.360 kb on - strand, within pdxI

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas14
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124,048 + MCAODC_16160 0.78 +1.6
124,157 - MCAODC_16160 0.82 -1.6
124,169 + MCAODC_16160 0.82 -1.1
124,210 + MCAODC_16160 0.83 -1.1
124,210 + MCAODC_16160 0.83 -0.2
124,210 + MCAODC_16160 0.83 -2.3
124,214 + MCAODC_16160 0.83 +0.1
124,330 + MCAODC_16160 0.87 +0.3
124,330 + MCAODC_16160 0.87 +0.3
124,342 + MCAODC_16160 0.88 +1.1
124,342 + MCAODC_16160 0.88 -0.6
124,391 + MCAODC_16160 0.89 +0.4
124,393 + MCAODC_16160 0.89 -3.2
124,561 + +1.0
124,700 - +0.7
124,730 + +1.0
124,748 + +0.8
124,762 - -0.3
124,803 + +1.2
124,806 + +0.4
124,971 + +1.5
124,971 + +0.2
125,061 + +0.7
125,065 + -0.5
125,068 + -0.5
125,092 - +0.5
125,107 + +0.1
125,116 + -1.1
125,212 + MCAODC_16165 0.11 +0.4
125,388 + MCAODC_16165 0.18 -1.2
125,388 + MCAODC_16165 0.18 +0.3
125,390 + MCAODC_16165 0.18 -0.4
125,391 - MCAODC_16165 0.18 -0.0
125,392 + MCAODC_16165 0.18 +0.1
125,392 + MCAODC_16165 0.18 +0.8
125,392 + MCAODC_16165 0.18 -1.0
125,393 - MCAODC_16165 0.19 +2.4
125,424 + MCAODC_16165 0.20 +1.8
125,512 + MCAODC_16165 0.23 +0.6
125,569 + MCAODC_16165 0.26 +1.8
125,570 - MCAODC_16165 0.26 +0.5
125,618 + MCAODC_16165 0.28 +1.0
125,618 + MCAODC_16165 0.28 +0.2
125,793 + MCAODC_16165 0.35 -1.3
125,885 - MCAODC_16165 0.39 +3.1
125,994 - MCAODC_16165 0.43 -0.5
126,029 + MCAODC_16165 0.44 -0.7
126,029 + MCAODC_16165 0.44 -0.7
126,029 + MCAODC_16165 0.44 +0.0
126,029 + MCAODC_16165 0.44 +0.9
126,030 - MCAODC_16165 0.44 +1.2
126,070 + MCAODC_16165 0.46 +0.8
126,071 - MCAODC_16165 0.46 +0.2
126,101 + MCAODC_16165 0.47 +1.0
126,350 + MCAODC_16165 0.57 +0.5
126,428 + MCAODC_16165 0.61 +1.1
126,431 - MCAODC_16165 0.61 -0.5
126,641 + MCAODC_16165 0.69 +0.3
126,661 + MCAODC_16165 0.70 -1.3
126,662 - MCAODC_16165 0.70 +0.5
126,817 + MCAODC_16165 0.76 -1.9
127,295 + +0.2
127,295 + -0.3
127,295 + +0.4
127,331 + -0.6
127,473 - -0.5
127,553 - -0.1
127,557 - +0.0
127,573 - +0.8
127,575 - -0.3
127,601 - +0.3
127,627 + +0.2
127,634 - +0.2
127,634 - -0.3
127,791 - pdxI MCAODC_16170 0.20 +0.9
127,791 - pdxI MCAODC_16170 0.20 +0.5
128,169 - pdxI MCAODC_16170 0.64 +1.5
128,343 + pdxI MCAODC_16170 0.84 -2.6
128,360 - pdxI MCAODC_16170 0.86 +1.0

Or see this region's nucleotide sequence