Experiment: WM_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpQ and MCAODC_14125 are separated by 223 nucleotides MCAODC_14125 and MCAODC_14130 are separated by 178 nucleotides MCAODC_14130 and lysR are separated by 153 nucleotides
MCAODC_14120: glpQ - glycerophosphodiester phosphodiesterase, at 2,767,337 to 2,768,413
glpQ
MCAODC_14125: MCAODC_14125 - hypothetical protein, at 2,768,637 to 2,769,707
_14125
MCAODC_14130: MCAODC_14130 - hypothetical protein, at 2,769,886 to 2,770,017
_14130
MCAODC_14135: lysR - transcriptional regulator, at 2,770,171 to 2,771,049
lysR
Position (kb)
2768
2769
2770 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2767.640 kb on + strand, within glpQ at 2767.643 kb on + strand, within glpQ at 2767.730 kb on + strand, within glpQ at 2767.753 kb on + strand, within glpQ at 2767.753 kb on + strand, within glpQ at 2767.753 kb on + strand, within glpQ at 2767.753 kb on + strand, within glpQ at 2767.837 kb on + strand, within glpQ at 2767.837 kb on + strand, within glpQ at 2767.841 kb on + strand, within glpQ at 2767.841 kb on + strand, within glpQ at 2767.842 kb on - strand, within glpQ at 2767.842 kb on - strand, within glpQ at 2767.922 kb on + strand, within glpQ at 2767.923 kb on - strand, within glpQ at 2767.923 kb on - strand, within glpQ at 2767.988 kb on - strand, within glpQ at 2768.034 kb on + strand, within glpQ at 2768.048 kb on + strand, within glpQ at 2768.108 kb on + strand, within glpQ at 2768.182 kb on + strand, within glpQ at 2768.263 kb on - strand, within glpQ at 2768.274 kb on - strand, within glpQ at 2768.325 kb on + strand at 2768.325 kb on + strand at 2768.327 kb on + strand at 2768.328 kb on - strand at 2768.355 kb on + strand at 2768.465 kb on - strand at 2768.594 kb on - strand at 2768.683 kb on - strand at 2769.534 kb on + strand, within MCAODC_14125 at 2769.534 kb on + strand, within MCAODC_14125 at 2769.672 kb on - strand at 2769.740 kb on - strand at 2769.847 kb on - strand at 2769.867 kb on + strand at 2769.884 kb on + strand at 2770.088 kb on + strand at 2770.089 kb on - strand at 2770.100 kb on + strand at 2770.112 kb on + strand at 2770.113 kb on - strand at 2770.113 kb on - strand at 2770.118 kb on + strand at 2770.118 kb on + strand at 2770.118 kb on + strand at 2770.119 kb on - strand at 2770.192 kb on + strand at 2770.192 kb on + strand at 2770.513 kb on - strand, within lysR at 2770.586 kb on + strand, within lysR at 2770.587 kb on - strand, within lysR
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas14 remove 2,767,640 + glpQ MCAODC_14120 0.28 +0.6 2,767,643 + glpQ MCAODC_14120 0.28 +1.9 2,767,730 + glpQ MCAODC_14120 0.36 +0.4 2,767,753 + glpQ MCAODC_14120 0.39 -0.2 2,767,753 + glpQ MCAODC_14120 0.39 -0.8 2,767,753 + glpQ MCAODC_14120 0.39 +0.3 2,767,753 + glpQ MCAODC_14120 0.39 +0.8 2,767,837 + glpQ MCAODC_14120 0.46 +0.3 2,767,837 + glpQ MCAODC_14120 0.46 -1.8 2,767,841 + glpQ MCAODC_14120 0.47 +1.2 2,767,841 + glpQ MCAODC_14120 0.47 +0.8 2,767,842 - glpQ MCAODC_14120 0.47 +0.8 2,767,842 - glpQ MCAODC_14120 0.47 +1.0 2,767,922 + glpQ MCAODC_14120 0.54 +0.9 2,767,923 - glpQ MCAODC_14120 0.54 -0.2 2,767,923 - glpQ MCAODC_14120 0.54 -0.2 2,767,988 - glpQ MCAODC_14120 0.60 +0.2 2,768,034 + glpQ MCAODC_14120 0.65 -0.3 2,768,048 + glpQ MCAODC_14120 0.66 +0.1 2,768,108 + glpQ MCAODC_14120 0.72 +0.8 2,768,182 + glpQ MCAODC_14120 0.78 +0.5 2,768,263 - glpQ MCAODC_14120 0.86 -0.6 2,768,274 - glpQ MCAODC_14120 0.87 -0.4 2,768,325 + -0.8 2,768,325 + +0.6 2,768,327 + +0.8 2,768,328 - -1.8 2,768,355 + +0.2 2,768,465 - +0.8 2,768,594 - +0.2 2,768,683 - -1.7 2,769,534 + MCAODC_14125 0.84 +0.8 2,769,534 + MCAODC_14125 0.84 +0.9 2,769,672 - +0.4 2,769,740 - -0.6 2,769,847 - -0.6 2,769,867 + +0.9 2,769,884 + -1.0 2,770,088 + +0.3 2,770,089 - +0.3 2,770,100 + +1.0 2,770,112 + -0.9 2,770,113 - +0.2 2,770,113 - -0.1 2,770,118 + +1.8 2,770,118 + -0.6 2,770,118 + -1.9 2,770,119 - +2.4 2,770,192 + +1.0 2,770,192 + -0.8 2,770,513 - lysR MCAODC_14135 0.39 +0.1 2,770,586 + lysR MCAODC_14135 0.47 +0.1 2,770,587 - lysR MCAODC_14135 0.47 +0.9
Or see this region's nucleotide sequence