Strain Fitness in Escherichia coli ECRC101 around MCAODC_09915

Experiment: WM_Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpgk and fbaA are separated by 214 nucleotidesfbaA and mscS are separated by 357 nucleotides MCAODC_09910: pgk - phosphoglycerate kinase, at 1,974,699 to 1,975,862 pgk MCAODC_09915: fbaA - class II fructose-bisphosphate aldolase, at 1,976,077 to 1,977,156 fbaA MCAODC_09925: mscS - small-conductance mechanosensitive channel MscS, at 1,977,514 to 1,978,374 mscS Position (kb) 1976 1977 1978Strain fitness (log2 ratio) -3 -2 -1 0 1at 1975.819 kb on + strandat 1975.853 kb on + strandat 1975.853 kb on + strandat 1975.853 kb on + strandat 1975.860 kb on + strandat 1976.047 kb on + strandat 1976.047 kb on + strandat 1976.049 kb on + strandat 1976.049 kb on + strandat 1977.154 kb on + strandat 1977.155 kb on - strandat 1977.173 kb on + strandat 1977.173 kb on + strandat 1977.174 kb on - strandat 1977.174 kb on - strandat 1977.239 kb on - strandat 1977.338 kb on + strandat 1977.339 kb on - strandat 1977.339 kb on - strandat 1977.411 kb on + strandat 1977.412 kb on - strandat 1977.424 kb on + strandat 1977.431 kb on + strandat 1977.432 kb on - strandat 1977.432 kb on - strandat 1977.432 kb on - strandat 1977.432 kb on - strandat 1977.476 kb on + strandat 1977.508 kb on + strandat 1977.508 kb on + strandat 1977.508 kb on + strandat 1977.567 kb on - strandat 1977.600 kb on - strand, within mscSat 1977.600 kb on - strand, within mscSat 1977.728 kb on + strand, within mscSat 1977.744 kb on + strand, within mscSat 1977.827 kb on + strand, within mscSat 1977.827 kb on + strand, within mscSat 1977.828 kb on - strand, within mscSat 1977.828 kb on - strand, within mscSat 1977.862 kb on - strand, within mscSat 1977.882 kb on - strand, within mscSat 1977.916 kb on + strand, within mscSat 1977.917 kb on - strand, within mscSat 1977.917 kb on - strand, within mscSat 1977.942 kb on + strand, within mscSat 1978.017 kb on + strand, within mscSat 1978.035 kb on + strand, within mscSat 1978.041 kb on + strand, within mscSat 1978.042 kb on - strand, within mscSat 1978.077 kb on + strand, within mscSat 1978.077 kb on + strand, within mscSat 1978.080 kb on + strand, within mscSat 1978.093 kb on + strand, within mscSat 1978.134 kb on + strand, within mscSat 1978.137 kb on + strand, within mscS

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas14
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1,975,819 + -0.6
1,975,853 + -0.4
1,975,853 + -1.2
1,975,853 + -0.7
1,975,860 + -0.6
1,976,047 + -0.3
1,976,047 + -1.3
1,976,049 + -3.6
1,976,049 + +0.1
1,977,154 + -0.5
1,977,155 - -0.6
1,977,173 + +0.2
1,977,173 + +0.1
1,977,174 - +1.4
1,977,174 - -0.0
1,977,239 - -0.6
1,977,338 + +1.2
1,977,339 - +0.2
1,977,339 - -0.7
1,977,411 + +0.0
1,977,412 - +0.6
1,977,424 + +0.3
1,977,431 + -0.8
1,977,432 - -1.1
1,977,432 - -0.7
1,977,432 - +0.8
1,977,432 - -0.3
1,977,476 + -0.2
1,977,508 + -0.3
1,977,508 + +0.1
1,977,508 + +0.1
1,977,567 - +0.5
1,977,600 - mscS MCAODC_09925 0.10 -0.7
1,977,600 - mscS MCAODC_09925 0.10 +0.2
1,977,728 + mscS MCAODC_09925 0.25 -0.7
1,977,744 + mscS MCAODC_09925 0.27 -0.0
1,977,827 + mscS MCAODC_09925 0.36 +0.9
1,977,827 + mscS MCAODC_09925 0.36 +0.5
1,977,828 - mscS MCAODC_09925 0.36 -0.4
1,977,828 - mscS MCAODC_09925 0.36 +0.3
1,977,862 - mscS MCAODC_09925 0.40 -0.0
1,977,882 - mscS MCAODC_09925 0.43 -0.2
1,977,916 + mscS MCAODC_09925 0.47 +0.3
1,977,917 - mscS MCAODC_09925 0.47 -0.6
1,977,917 - mscS MCAODC_09925 0.47 +0.0
1,977,942 + mscS MCAODC_09925 0.50 +0.8
1,978,017 + mscS MCAODC_09925 0.58 +0.4
1,978,035 + mscS MCAODC_09925 0.61 -0.4
1,978,041 + mscS MCAODC_09925 0.61 +0.3
1,978,042 - mscS MCAODC_09925 0.61 -0.7
1,978,077 + mscS MCAODC_09925 0.65 +0.5
1,978,077 + mscS MCAODC_09925 0.65 -0.6
1,978,080 + mscS MCAODC_09925 0.66 +0.0
1,978,093 + mscS MCAODC_09925 0.67 -0.6
1,978,134 + mscS MCAODC_09925 0.72 -1.1
1,978,137 + mscS MCAODC_09925 0.72 -2.2

Or see this region's nucleotide sequence