Experiment: WM_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acrS and yhdU are separated by 2 nucleotides yhdU and yhdJ are separated by 83 nucleotides yhdJ and fis are separated by 84 nucleotides fis and dusB are separated by 25 nucleotides
MCAODC_08185: acrS - multidrug efflux transporter transcriptional repressor AcrS, at 1,637,771 to 1,638,433
acrS
MCAODC_08190: yhdU - Inner membrane protein YhdU, at 1,638,436 to 1,638,615
yhdU
MCAODC_08195: yhdJ - adenine-specific DNA-methyltransferase, at 1,638,699 to 1,639,583
yhdJ
MCAODC_08200: fis - DNA-binding transcriptional regulator Fis, at 1,639,668 to 1,639,964
fis
MCAODC_08205: dusB - tRNA dihydrouridine synthase DusB, at 1,639,990 to 1,640,955
dusB
Position (kb) 1638
1639
1640 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1637.899 kb on + strand, within acrS at 1637.912 kb on - strand, within acrS at 1637.912 kb on - strand, within acrS at 1637.915 kb on + strand, within acrS at 1637.916 kb on - strand, within acrS at 1637.916 kb on - strand, within acrS at 1637.916 kb on - strand, within acrS at 1637.947 kb on + strand, within acrS at 1638.065 kb on + strand, within acrS at 1638.066 kb on - strand, within acrS at 1638.108 kb on + strand, within acrS at 1638.246 kb on + strand, within acrS at 1638.246 kb on + strand, within acrS at 1638.266 kb on - strand, within acrS at 1638.360 kb on + strand, within acrS at 1638.361 kb on - strand, within acrS at 1638.558 kb on + strand, within yhdU at 1638.602 kb on + strand at 1638.602 kb on + strand at 1638.602 kb on + strand at 1638.602 kb on + strand at 1638.603 kb on - strand at 1638.603 kb on - strand at 1638.616 kb on + strand at 1638.658 kb on + strand at 1638.689 kb on + strand at 1638.690 kb on - strand at 1638.690 kb on - strand at 1638.700 kb on + strand at 1638.707 kb on + strand at 1638.799 kb on + strand, within yhdJ at 1638.799 kb on + strand, within yhdJ at 1638.799 kb on + strand, within yhdJ at 1638.842 kb on - strand, within yhdJ at 1638.861 kb on - strand, within yhdJ at 1638.891 kb on + strand, within yhdJ at 1638.906 kb on - strand, within yhdJ at 1638.906 kb on - strand, within yhdJ at 1638.906 kb on - strand, within yhdJ at 1638.906 kb on - strand, within yhdJ at 1638.912 kb on - strand, within yhdJ at 1639.025 kb on - strand, within yhdJ at 1639.075 kb on + strand, within yhdJ at 1639.075 kb on + strand, within yhdJ at 1639.075 kb on + strand, within yhdJ at 1639.075 kb on + strand, within yhdJ at 1639.076 kb on - strand, within yhdJ at 1639.076 kb on - strand, within yhdJ at 1639.138 kb on - strand, within yhdJ at 1639.145 kb on + strand, within yhdJ at 1639.146 kb on - strand, within yhdJ at 1639.165 kb on + strand, within yhdJ at 1639.204 kb on + strand, within yhdJ at 1639.204 kb on + strand, within yhdJ at 1639.205 kb on - strand, within yhdJ at 1639.205 kb on - strand, within yhdJ at 1639.205 kb on - strand, within yhdJ at 1639.205 kb on - strand, within yhdJ at 1639.205 kb on - strand, within yhdJ at 1639.205 kb on - strand, within yhdJ at 1639.249 kb on + strand, within yhdJ at 1639.277 kb on + strand, within yhdJ at 1639.336 kb on + strand, within yhdJ at 1639.336 kb on + strand, within yhdJ at 1639.336 kb on + strand, within yhdJ at 1639.336 kb on + strand, within yhdJ at 1639.336 kb on + strand, within yhdJ at 1639.336 kb on + strand, within yhdJ at 1639.337 kb on - strand, within yhdJ at 1639.337 kb on - strand, within yhdJ at 1639.387 kb on - strand, within yhdJ at 1639.454 kb on + strand, within yhdJ at 1639.455 kb on - strand, within yhdJ at 1639.455 kb on - strand, within yhdJ at 1639.456 kb on + strand, within yhdJ at 1639.456 kb on + strand, within yhdJ at 1639.457 kb on - strand, within yhdJ at 1639.457 kb on - strand, within yhdJ at 1639.457 kb on - strand, within yhdJ at 1639.457 kb on - strand, within yhdJ at 1639.497 kb on + strand at 1639.498 kb on - strand at 1639.498 kb on - strand at 1639.505 kb on + strand at 1639.506 kb on - strand at 1639.532 kb on + strand at 1639.542 kb on - strand at 1639.542 kb on - strand at 1639.542 kb on - strand at 1639.542 kb on - strand at 1639.644 kb on - strand at 1639.656 kb on - strand at 1639.656 kb on - strand at 1639.670 kb on - strand at 1639.681 kb on + strand at 1639.759 kb on + strand, within fis at 1639.759 kb on + strand, within fis at 1639.814 kb on - strand, within fis at 1639.853 kb on - strand, within fis at 1639.935 kb on + strand at 1639.948 kb on - strand at 1639.965 kb on - strand at 1639.965 kb on - strand at 1639.965 kb on - strand at 1639.965 kb on - strand at 1639.982 kb on - strand at 1640.010 kb on - strand at 1640.010 kb on - strand at 1640.010 kb on - strand at 1640.010 kb on - strand at 1640.116 kb on - strand, within dusB at 1640.116 kb on - strand, within dusB at 1640.116 kb on - strand, within dusB at 1640.147 kb on + strand, within dusB at 1640.148 kb on - strand, within dusB at 1640.191 kb on - strand, within dusB at 1640.238 kb on - strand, within dusB at 1640.238 kb on - strand, within dusB at 1640.288 kb on - strand, within dusB at 1640.288 kb on - strand, within dusB at 1640.288 kb on - strand, within dusB at 1640.288 kb on - strand, within dusB at 1640.310 kb on + strand, within dusB at 1640.311 kb on - strand, within dusB at 1640.312 kb on + strand, within dusB at 1640.313 kb on - strand, within dusB at 1640.347 kb on - strand, within dusB at 1640.347 kb on - strand, within dusB at 1640.388 kb on + strand, within dusB at 1640.388 kb on + strand, within dusB at 1640.389 kb on - strand, within dusB at 1640.389 kb on - strand, within dusB at 1640.389 kb on - strand, within dusB at 1640.401 kb on - strand, within dusB at 1640.401 kb on - strand, within dusB at 1640.508 kb on + strand, within dusB
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas14 remove 1,637,899 + acrS MCAODC_08185 0.19 -0.3 1,637,912 - acrS MCAODC_08185 0.21 +0.5 1,637,912 - acrS MCAODC_08185 0.21 -0.2 1,637,915 + acrS MCAODC_08185 0.22 -1.1 1,637,916 - acrS MCAODC_08185 0.22 +0.4 1,637,916 - acrS MCAODC_08185 0.22 -2.0 1,637,916 - acrS MCAODC_08185 0.22 +0.5 1,637,947 + acrS MCAODC_08185 0.27 -0.3 1,638,065 + acrS MCAODC_08185 0.44 -0.3 1,638,066 - acrS MCAODC_08185 0.44 -0.4 1,638,108 + acrS MCAODC_08185 0.51 -0.2 1,638,246 + acrS MCAODC_08185 0.72 -0.5 1,638,246 + acrS MCAODC_08185 0.72 -1.9 1,638,266 - acrS MCAODC_08185 0.75 +0.7 1,638,360 + acrS MCAODC_08185 0.89 -0.1 1,638,361 - acrS MCAODC_08185 0.89 -0.0 1,638,558 + yhdU MCAODC_08190 0.68 +0.9 1,638,602 + +0.6 1,638,602 + -0.4 1,638,602 + +0.8 1,638,602 + -1.5 1,638,603 - -0.4 1,638,603 - +0.3 1,638,616 + -0.1 1,638,658 + -0.1 1,638,689 + +0.1 1,638,690 - -1.3 1,638,690 - -1.6 1,638,700 + -0.6 1,638,707 + -0.5 1,638,799 + yhdJ MCAODC_08195 0.11 +0.9 1,638,799 + yhdJ MCAODC_08195 0.11 -0.1 1,638,799 + yhdJ MCAODC_08195 0.11 -1.0 1,638,842 - yhdJ MCAODC_08195 0.16 +1.0 1,638,861 - yhdJ MCAODC_08195 0.18 +0.5 1,638,891 + yhdJ MCAODC_08195 0.22 +0.1 1,638,906 - yhdJ MCAODC_08195 0.23 -0.4 1,638,906 - yhdJ MCAODC_08195 0.23 +0.1 1,638,906 - yhdJ MCAODC_08195 0.23 -0.1 1,638,906 - yhdJ MCAODC_08195 0.23 +0.3 1,638,912 - yhdJ MCAODC_08195 0.24 +1.8 1,639,025 - yhdJ MCAODC_08195 0.37 +0.3 1,639,075 + yhdJ MCAODC_08195 0.42 +0.1 1,639,075 + yhdJ MCAODC_08195 0.42 +1.7 1,639,075 + yhdJ MCAODC_08195 0.42 +1.4 1,639,075 + yhdJ MCAODC_08195 0.42 -0.6 1,639,076 - yhdJ MCAODC_08195 0.43 -0.2 1,639,076 - yhdJ MCAODC_08195 0.43 +1.6 1,639,138 - yhdJ MCAODC_08195 0.50 -0.1 1,639,145 + yhdJ MCAODC_08195 0.50 +0.2 1,639,146 - yhdJ MCAODC_08195 0.51 +0.9 1,639,165 + yhdJ MCAODC_08195 0.53 +1.7 1,639,204 + yhdJ MCAODC_08195 0.57 +0.8 1,639,204 + yhdJ MCAODC_08195 0.57 +1.1 1,639,205 - yhdJ MCAODC_08195 0.57 -0.8 1,639,205 - yhdJ MCAODC_08195 0.57 +0.9 1,639,205 - yhdJ MCAODC_08195 0.57 +0.5 1,639,205 - yhdJ MCAODC_08195 0.57 +1.3 1,639,205 - yhdJ MCAODC_08195 0.57 -0.9 1,639,205 - yhdJ MCAODC_08195 0.57 -0.2 1,639,249 + yhdJ MCAODC_08195 0.62 +1.0 1,639,277 + yhdJ MCAODC_08195 0.65 -0.0 1,639,336 + yhdJ MCAODC_08195 0.72 -0.5 1,639,336 + yhdJ MCAODC_08195 0.72 +0.4 1,639,336 + yhdJ MCAODC_08195 0.72 -1.5 1,639,336 + yhdJ MCAODC_08195 0.72 +0.5 1,639,336 + yhdJ MCAODC_08195 0.72 +0.0 1,639,336 + yhdJ MCAODC_08195 0.72 -1.1 1,639,337 - yhdJ MCAODC_08195 0.72 +0.4 1,639,337 - yhdJ MCAODC_08195 0.72 +0.9 1,639,387 - yhdJ MCAODC_08195 0.78 +1.1 1,639,454 + yhdJ MCAODC_08195 0.85 +0.9 1,639,455 - yhdJ MCAODC_08195 0.85 -0.2 1,639,455 - yhdJ MCAODC_08195 0.85 -1.2 1,639,456 + yhdJ MCAODC_08195 0.86 -0.1 1,639,456 + yhdJ MCAODC_08195 0.86 +1.9 1,639,457 - yhdJ MCAODC_08195 0.86 +0.7 1,639,457 - yhdJ MCAODC_08195 0.86 -1.4 1,639,457 - yhdJ MCAODC_08195 0.86 +0.4 1,639,457 - yhdJ MCAODC_08195 0.86 -0.4 1,639,497 + +0.2 1,639,498 - +0.6 1,639,498 - -0.8 1,639,505 + +1.3 1,639,506 - -2.7 1,639,532 + +0.0 1,639,542 - -1.5 1,639,542 - -1.1 1,639,542 - +0.5 1,639,542 - +0.3 1,639,644 - +0.2 1,639,656 - +0.8 1,639,656 - +0.9 1,639,670 - +0.2 1,639,681 + -2.0 1,639,759 + fis MCAODC_08200 0.31 -0.2 1,639,759 + fis MCAODC_08200 0.31 -1.5 1,639,814 - fis MCAODC_08200 0.49 -0.3 1,639,853 - fis MCAODC_08200 0.62 +0.1 1,639,935 + -0.5 1,639,948 - -0.0 1,639,965 - +3.7 1,639,965 - +0.5 1,639,965 - +1.4 1,639,965 - -0.8 1,639,982 - +0.8 1,640,010 - +0.1 1,640,010 - +1.8 1,640,010 - +0.0 1,640,010 - -1.0 1,640,116 - dusB MCAODC_08205 0.13 +0.4 1,640,116 - dusB MCAODC_08205 0.13 +0.9 1,640,116 - dusB MCAODC_08205 0.13 -0.5 1,640,147 + dusB MCAODC_08205 0.16 +0.2 1,640,148 - dusB MCAODC_08205 0.16 +0.4 1,640,191 - dusB MCAODC_08205 0.21 -0.3 1,640,238 - dusB MCAODC_08205 0.26 +0.3 1,640,238 - dusB MCAODC_08205 0.26 -0.5 1,640,288 - dusB MCAODC_08205 0.31 -1.6 1,640,288 - dusB MCAODC_08205 0.31 -0.4 1,640,288 - dusB MCAODC_08205 0.31 +0.7 1,640,288 - dusB MCAODC_08205 0.31 +0.3 1,640,310 + dusB MCAODC_08205 0.33 -0.6 1,640,311 - dusB MCAODC_08205 0.33 +0.6 1,640,312 + dusB MCAODC_08205 0.33 +0.0 1,640,313 - dusB MCAODC_08205 0.33 -0.1 1,640,347 - dusB MCAODC_08205 0.37 +0.9 1,640,347 - dusB MCAODC_08205 0.37 +0.4 1,640,388 + dusB MCAODC_08205 0.41 +1.1 1,640,388 + dusB MCAODC_08205 0.41 +0.1 1,640,389 - dusB MCAODC_08205 0.41 -1.2 1,640,389 - dusB MCAODC_08205 0.41 -3.4 1,640,389 - dusB MCAODC_08205 0.41 +0.0 1,640,401 - dusB MCAODC_08205 0.43 +0.3 1,640,401 - dusB MCAODC_08205 0.43 +0.0 1,640,508 + dusB MCAODC_08205 0.54 -1.2
Or see this region's nucleotide sequence