Experiment: WM_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ytfF and ytfE are separated by 107 nucleotides ytfE and cycA are separated by 44 nucleotides cycA and fklB are separated by 308 nucleotides
MCAODC_02575: ytfF - Inner membrane protein YtfF, at 525,535 to 526,500
ytfF
MCAODC_02580: ytfE - iron-sulfur cluster repair protein YtfE, at 526,608 to 527,270
ytfE
MCAODC_02585: cycA - D-serine/D-alanine/glycine transporter, at 527,315 to 528,727
cycA
MCAODC_02590: fklB - FKBP-type peptidyl-prolyl cis-trans isomerase, at 529,036 to 529,656
fklB
Position (kb) 527
528
529 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 526.366 kb on + strand, within ytfF at 526.367 kb on - strand, within ytfF at 526.522 kb on - strand at 526.532 kb on + strand at 526.533 kb on - strand at 526.536 kb on + strand at 526.543 kb on + strand at 526.587 kb on - strand at 526.589 kb on + strand at 526.589 kb on + strand at 526.590 kb on - strand at 526.607 kb on + strand at 526.607 kb on + strand at 526.608 kb on - strand at 526.608 kb on - strand at 526.630 kb on + strand at 526.630 kb on + strand at 526.630 kb on + strand at 526.631 kb on - strand at 526.649 kb on + strand at 526.650 kb on - strand at 526.677 kb on - strand, within ytfE at 526.680 kb on + strand, within ytfE at 526.680 kb on + strand, within ytfE at 526.680 kb on + strand, within ytfE at 526.680 kb on + strand, within ytfE at 526.680 kb on + strand, within ytfE at 526.681 kb on - strand, within ytfE at 526.681 kb on - strand, within ytfE at 526.681 kb on - strand, within ytfE at 526.692 kb on + strand, within ytfE at 526.736 kb on + strand, within ytfE at 526.736 kb on + strand, within ytfE at 526.841 kb on + strand, within ytfE at 526.842 kb on - strand, within ytfE at 527.072 kb on - strand, within ytfE at 527.126 kb on + strand, within ytfE at 527.127 kb on - strand, within ytfE at 527.132 kb on + strand, within ytfE at 527.178 kb on + strand, within ytfE at 527.178 kb on + strand, within ytfE at 527.178 kb on + strand, within ytfE at 527.178 kb on + strand, within ytfE at 527.178 kb on + strand, within ytfE at 527.178 kb on + strand, within ytfE at 527.178 kb on + strand, within ytfE at 527.178 kb on + strand, within ytfE at 527.179 kb on - strand, within ytfE at 527.179 kb on - strand, within ytfE at 527.190 kb on - strand, within ytfE at 527.190 kb on - strand, within ytfE at 527.239 kb on + strand at 527.239 kb on + strand at 527.239 kb on + strand at 527.239 kb on + strand at 527.240 kb on - strand at 527.240 kb on - strand at 527.273 kb on + strand at 527.307 kb on + strand at 527.308 kb on - strand at 527.308 kb on - strand at 527.308 kb on - strand at 527.308 kb on - strand at 527.344 kb on + strand at 527.345 kb on - strand at 527.350 kb on + strand at 527.461 kb on + strand, within cycA at 527.461 kb on + strand, within cycA at 527.461 kb on + strand, within cycA at 527.461 kb on + strand, within cycA at 527.462 kb on - strand, within cycA at 527.462 kb on - strand, within cycA at 527.465 kb on + strand, within cycA at 527.465 kb on + strand, within cycA at 527.465 kb on + strand, within cycA at 527.466 kb on - strand, within cycA at 527.499 kb on + strand, within cycA at 527.500 kb on - strand, within cycA at 527.500 kb on - strand, within cycA at 527.516 kb on + strand, within cycA at 527.516 kb on + strand, within cycA at 527.517 kb on - strand, within cycA at 527.517 kb on - strand, within cycA at 527.538 kb on + strand, within cycA at 527.539 kb on - strand, within cycA at 527.548 kb on - strand, within cycA at 527.578 kb on + strand, within cycA at 527.579 kb on - strand, within cycA at 527.603 kb on - strand, within cycA at 527.603 kb on - strand, within cycA at 527.603 kb on - strand, within cycA at 527.640 kb on + strand, within cycA at 527.640 kb on + strand, within cycA at 527.640 kb on + strand, within cycA at 527.640 kb on + strand, within cycA at 527.640 kb on + strand, within cycA at 527.641 kb on - strand, within cycA at 527.671 kb on + strand, within cycA at 527.671 kb on + strand, within cycA at 527.671 kb on + strand, within cycA at 527.671 kb on + strand, within cycA at 527.671 kb on + strand, within cycA at 527.672 kb on - strand, within cycA at 527.776 kb on + strand, within cycA at 527.859 kb on - strand, within cycA at 527.859 kb on - strand, within cycA at 527.859 kb on - strand, within cycA at 527.859 kb on - strand, within cycA at 527.901 kb on - strand, within cycA at 527.926 kb on + strand, within cycA at 527.926 kb on + strand, within cycA at 527.926 kb on + strand, within cycA at 527.927 kb on - strand, within cycA at 527.927 kb on - strand, within cycA at 527.927 kb on - strand, within cycA at 527.927 kb on - strand, within cycA at 527.929 kb on + strand, within cycA at 527.929 kb on + strand, within cycA at 527.929 kb on + strand, within cycA at 527.929 kb on + strand, within cycA at 527.930 kb on - strand, within cycA at 527.949 kb on + strand, within cycA at 527.956 kb on + strand, within cycA at 527.956 kb on + strand, within cycA at 527.956 kb on + strand, within cycA at 527.956 kb on + strand, within cycA at 527.956 kb on + strand, within cycA at 527.957 kb on - strand, within cycA at 527.957 kb on - strand, within cycA at 527.957 kb on - strand, within cycA at 527.957 kb on - strand, within cycA at 528.031 kb on + strand, within cycA at 528.031 kb on + strand, within cycA at 528.032 kb on - strand, within cycA at 528.032 kb on - strand, within cycA at 528.069 kb on - strand, within cycA at 528.095 kb on + strand, within cycA at 528.095 kb on + strand, within cycA at 528.095 kb on + strand, within cycA at 528.096 kb on - strand, within cycA at 528.096 kb on - strand, within cycA at 528.096 kb on - strand, within cycA at 528.096 kb on - strand, within cycA at 528.280 kb on + strand, within cycA at 528.354 kb on + strand, within cycA at 528.354 kb on + strand, within cycA at 528.355 kb on - strand, within cycA at 528.355 kb on - strand, within cycA at 528.355 kb on - strand, within cycA at 528.355 kb on - strand, within cycA at 528.389 kb on + strand, within cycA at 528.427 kb on - strand, within cycA at 528.478 kb on - strand, within cycA at 528.533 kb on - strand, within cycA at 528.533 kb on - strand, within cycA at 528.547 kb on + strand, within cycA at 528.547 kb on + strand, within cycA at 528.547 kb on + strand, within cycA at 528.548 kb on - strand, within cycA at 528.548 kb on - strand, within cycA at 528.548 kb on - strand, within cycA at 528.548 kb on - strand, within cycA at 528.548 kb on - strand, within cycA at 528.548 kb on - strand, within cycA at 528.548 kb on - strand, within cycA at 528.549 kb on + strand, within cycA at 528.550 kb on - strand, within cycA at 528.638 kb on - strand at 528.647 kb on - strand at 528.722 kb on + strand at 528.722 kb on + strand at 528.722 kb on + strand at 528.722 kb on + strand at 528.723 kb on - strand at 528.723 kb on - strand at 528.723 kb on - strand at 528.723 kb on - strand at 528.723 kb on - strand at 528.743 kb on + strand at 528.768 kb on - strand at 528.777 kb on - strand at 528.785 kb on + strand at 528.786 kb on - strand at 528.791 kb on - strand at 528.822 kb on + strand at 528.822 kb on + strand at 528.822 kb on + strand at 528.823 kb on - strand at 528.823 kb on - strand at 528.823 kb on - strand at 528.823 kb on - strand at 528.827 kb on - strand at 528.827 kb on - strand at 528.862 kb on - strand at 528.868 kb on - strand at 528.874 kb on - strand at 528.879 kb on + strand at 528.880 kb on - strand at 528.936 kb on - strand at 529.037 kb on + strand at 529.116 kb on - strand, within fklB at 529.134 kb on + strand, within fklB at 529.135 kb on - strand, within fklB at 529.135 kb on - strand, within fklB at 529.157 kb on - strand, within fklB at 529.269 kb on + strand, within fklB at 529.277 kb on + strand, within fklB at 529.278 kb on - strand, within fklB at 529.278 kb on - strand, within fklB at 529.278 kb on - strand, within fklB at 529.278 kb on - strand, within fklB at 529.300 kb on - strand, within fklB at 529.300 kb on - strand, within fklB at 529.363 kb on - strand, within fklB at 529.363 kb on - strand, within fklB at 529.533 kb on + strand, within fklB at 529.533 kb on + strand, within fklB at 529.659 kb on + strand at 529.676 kb on + strand at 529.676 kb on + strand at 529.677 kb on - strand at 529.677 kb on - strand at 529.678 kb on + strand at 529.678 kb on + strand at 529.679 kb on - strand at 529.679 kb on - strand at 529.683 kb on + strand at 529.696 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas14 remove 526,366 + ytfF MCAODC_02575 0.86 -0.6 526,367 - ytfF MCAODC_02575 0.86 -1.0 526,522 - -0.6 526,532 + +0.2 526,533 - +0.3 526,536 + -0.6 526,543 + -0.3 526,587 - +0.7 526,589 + +0.8 526,589 + +0.1 526,590 - +0.5 526,607 + -0.1 526,607 + +2.1 526,608 - -0.2 526,608 - -0.7 526,630 + -1.2 526,630 + -0.2 526,630 + +0.2 526,631 - +0.9 526,649 + +0.1 526,650 - +0.1 526,677 - ytfE MCAODC_02580 0.10 +0.1 526,680 + ytfE MCAODC_02580 0.11 -1.4 526,680 + ytfE MCAODC_02580 0.11 -0.3 526,680 + ytfE MCAODC_02580 0.11 -1.2 526,680 + ytfE MCAODC_02580 0.11 +0.0 526,680 + ytfE MCAODC_02580 0.11 +0.2 526,681 - ytfE MCAODC_02580 0.11 -0.7 526,681 - ytfE MCAODC_02580 0.11 -0.2 526,681 - ytfE MCAODC_02580 0.11 +0.3 526,692 + ytfE MCAODC_02580 0.13 +0.4 526,736 + ytfE MCAODC_02580 0.19 -1.6 526,736 + ytfE MCAODC_02580 0.19 +0.8 526,841 + ytfE MCAODC_02580 0.35 +1.4 526,842 - ytfE MCAODC_02580 0.35 +0.4 527,072 - ytfE MCAODC_02580 0.70 -1.2 527,126 + ytfE MCAODC_02580 0.78 -0.5 527,127 - ytfE MCAODC_02580 0.78 +1.0 527,132 + ytfE MCAODC_02580 0.79 -2.3 527,178 + ytfE MCAODC_02580 0.86 +0.4 527,178 + ytfE MCAODC_02580 0.86 +0.3 527,178 + ytfE MCAODC_02580 0.86 -0.3 527,178 + ytfE MCAODC_02580 0.86 -0.4 527,178 + ytfE MCAODC_02580 0.86 +0.2 527,178 + ytfE MCAODC_02580 0.86 +0.0 527,178 + ytfE MCAODC_02580 0.86 +0.4 527,178 + ytfE MCAODC_02580 0.86 -1.3 527,179 - ytfE MCAODC_02580 0.86 -0.3 527,179 - ytfE MCAODC_02580 0.86 +0.8 527,190 - ytfE MCAODC_02580 0.88 -0.1 527,190 - ytfE MCAODC_02580 0.88 +0.4 527,239 + -0.6 527,239 + -0.5 527,239 + +0.5 527,239 + +0.3 527,240 - -0.2 527,240 - -1.0 527,273 + -0.8 527,307 + +0.1 527,308 - +0.2 527,308 - +0.4 527,308 - +1.1 527,308 - -1.4 527,344 + -2.9 527,345 - -1.2 527,350 + +0.0 527,461 + cycA MCAODC_02585 0.10 +2.2 527,461 + cycA MCAODC_02585 0.10 +0.8 527,461 + cycA MCAODC_02585 0.10 +0.6 527,461 + cycA MCAODC_02585 0.10 -0.4 527,462 - cycA MCAODC_02585 0.10 -0.2 527,462 - cycA MCAODC_02585 0.10 +0.3 527,465 + cycA MCAODC_02585 0.11 +0.4 527,465 + cycA MCAODC_02585 0.11 -1.5 527,465 + cycA MCAODC_02585 0.11 +1.1 527,466 - cycA MCAODC_02585 0.11 +0.4 527,499 + cycA MCAODC_02585 0.13 -0.0 527,500 - cycA MCAODC_02585 0.13 -0.7 527,500 - cycA MCAODC_02585 0.13 -0.4 527,516 + cycA MCAODC_02585 0.14 +0.4 527,516 + cycA MCAODC_02585 0.14 +0.5 527,517 - cycA MCAODC_02585 0.14 +0.7 527,517 - cycA MCAODC_02585 0.14 -0.3 527,538 + cycA MCAODC_02585 0.16 +0.1 527,539 - cycA MCAODC_02585 0.16 +0.1 527,548 - cycA MCAODC_02585 0.16 +0.6 527,578 + cycA MCAODC_02585 0.19 -0.2 527,579 - cycA MCAODC_02585 0.19 -1.5 527,603 - cycA MCAODC_02585 0.20 -2.4 527,603 - cycA MCAODC_02585 0.20 -0.2 527,603 - cycA MCAODC_02585 0.20 +0.5 527,640 + cycA MCAODC_02585 0.23 +0.6 527,640 + cycA MCAODC_02585 0.23 +0.3 527,640 + cycA MCAODC_02585 0.23 +0.5 527,640 + cycA MCAODC_02585 0.23 +0.5 527,640 + cycA MCAODC_02585 0.23 -0.7 527,641 - cycA MCAODC_02585 0.23 +0.3 527,671 + cycA MCAODC_02585 0.25 +2.3 527,671 + cycA MCAODC_02585 0.25 +0.3 527,671 + cycA MCAODC_02585 0.25 +0.1 527,671 + cycA MCAODC_02585 0.25 -0.2 527,671 + cycA MCAODC_02585 0.25 -0.8 527,672 - cycA MCAODC_02585 0.25 -0.2 527,776 + cycA MCAODC_02585 0.33 -0.9 527,859 - cycA MCAODC_02585 0.38 -0.1 527,859 - cycA MCAODC_02585 0.38 -0.0 527,859 - cycA MCAODC_02585 0.38 -0.2 527,859 - cycA MCAODC_02585 0.38 +0.7 527,901 - cycA MCAODC_02585 0.41 -0.2 527,926 + cycA MCAODC_02585 0.43 -1.1 527,926 + cycA MCAODC_02585 0.43 -0.3 527,926 + cycA MCAODC_02585 0.43 -0.9 527,927 - cycA MCAODC_02585 0.43 +0.5 527,927 - cycA MCAODC_02585 0.43 -1.0 527,927 - cycA MCAODC_02585 0.43 +1.3 527,927 - cycA MCAODC_02585 0.43 -0.8 527,929 + cycA MCAODC_02585 0.43 +0.1 527,929 + cycA MCAODC_02585 0.43 -1.2 527,929 + cycA MCAODC_02585 0.43 -0.0 527,929 + cycA MCAODC_02585 0.43 -0.8 527,930 - cycA MCAODC_02585 0.44 -0.8 527,949 + cycA MCAODC_02585 0.45 -0.3 527,956 + cycA MCAODC_02585 0.45 +0.3 527,956 + cycA MCAODC_02585 0.45 -0.6 527,956 + cycA MCAODC_02585 0.45 +0.2 527,956 + cycA MCAODC_02585 0.45 -0.0 527,956 + cycA MCAODC_02585 0.45 +0.5 527,957 - cycA MCAODC_02585 0.45 +0.2 527,957 - cycA MCAODC_02585 0.45 +0.2 527,957 - cycA MCAODC_02585 0.45 -0.9 527,957 - cycA MCAODC_02585 0.45 +0.1 528,031 + cycA MCAODC_02585 0.51 +0.1 528,031 + cycA MCAODC_02585 0.51 +0.7 528,032 - cycA MCAODC_02585 0.51 -0.2 528,032 - cycA MCAODC_02585 0.51 -0.6 528,069 - cycA MCAODC_02585 0.53 +0.3 528,095 + cycA MCAODC_02585 0.55 -0.3 528,095 + cycA MCAODC_02585 0.55 +0.2 528,095 + cycA MCAODC_02585 0.55 -0.1 528,096 - cycA MCAODC_02585 0.55 -1.4 528,096 - cycA MCAODC_02585 0.55 +0.1 528,096 - cycA MCAODC_02585 0.55 +0.0 528,096 - cycA MCAODC_02585 0.55 +0.7 528,280 + cycA MCAODC_02585 0.68 +0.2 528,354 + cycA MCAODC_02585 0.74 -1.8 528,354 + cycA MCAODC_02585 0.74 -0.1 528,355 - cycA MCAODC_02585 0.74 +0.1 528,355 - cycA MCAODC_02585 0.74 +1.0 528,355 - cycA MCAODC_02585 0.74 +0.4 528,355 - cycA MCAODC_02585 0.74 +0.1 528,389 + cycA MCAODC_02585 0.76 +1.0 528,427 - cycA MCAODC_02585 0.79 +0.7 528,478 - cycA MCAODC_02585 0.82 +1.6 528,533 - cycA MCAODC_02585 0.86 +1.6 528,533 - cycA MCAODC_02585 0.86 -0.6 528,547 + cycA MCAODC_02585 0.87 -0.2 528,547 + cycA MCAODC_02585 0.87 +0.5 528,547 + cycA MCAODC_02585 0.87 +0.7 528,548 - cycA MCAODC_02585 0.87 +0.6 528,548 - cycA MCAODC_02585 0.87 +0.1 528,548 - cycA MCAODC_02585 0.87 -1.2 528,548 - cycA MCAODC_02585 0.87 +1.0 528,548 - cycA MCAODC_02585 0.87 +0.2 528,548 - cycA MCAODC_02585 0.87 +1.5 528,548 - cycA MCAODC_02585 0.87 +0.1 528,549 + cycA MCAODC_02585 0.87 -0.7 528,550 - cycA MCAODC_02585 0.87 -2.3 528,638 - +0.8 528,647 - -0.0 528,722 + -0.0 528,722 + +0.1 528,722 + -1.5 528,722 + +1.5 528,723 - +0.6 528,723 - -0.3 528,723 - +0.1 528,723 - -0.7 528,723 - -0.6 528,743 + +0.1 528,768 - +0.3 528,777 - -0.5 528,785 + +0.1 528,786 - +2.1 528,791 - -0.7 528,822 + -0.4 528,822 + +0.3 528,822 + +0.9 528,823 - +0.5 528,823 - +0.8 528,823 - +0.2 528,823 - +0.3 528,827 - -0.3 528,827 - +0.3 528,862 - -0.7 528,868 - +0.1 528,874 - +0.1 528,879 + +0.1 528,880 - +0.7 528,936 - +0.2 529,037 + +0.7 529,116 - fklB MCAODC_02590 0.13 -0.8 529,134 + fklB MCAODC_02590 0.16 -0.4 529,135 - fklB MCAODC_02590 0.16 +0.3 529,135 - fklB MCAODC_02590 0.16 +0.2 529,157 - fklB MCAODC_02590 0.19 +0.7 529,269 + fklB MCAODC_02590 0.38 +0.6 529,277 + fklB MCAODC_02590 0.39 -0.2 529,278 - fklB MCAODC_02590 0.39 -0.7 529,278 - fklB MCAODC_02590 0.39 -0.6 529,278 - fklB MCAODC_02590 0.39 -0.5 529,278 - fklB MCAODC_02590 0.39 +1.1 529,300 - fklB MCAODC_02590 0.43 -0.1 529,300 - fklB MCAODC_02590 0.43 -1.0 529,363 - fklB MCAODC_02590 0.53 -1.2 529,363 - fklB MCAODC_02590 0.53 -3.6 529,533 + fklB MCAODC_02590 0.80 +1.5 529,533 + fklB MCAODC_02590 0.80 -0.7 529,659 + +1.3 529,676 + -1.7 529,676 + +0.3 529,677 - +0.3 529,677 - +0.8 529,678 + -0.6 529,678 + +1.1 529,679 - -0.7 529,679 - +0.3 529,683 + +0.1 529,696 - -2.0
Or see this region's nucleotide sequence