Experiment: WM_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt leuC1 and leuD are separated by 10 nucleotides leuD and sgrR are separated by 327 nucleotides sgrR and MCAODC_01260 are separated by 155 nucleotides MCAODC_01260 and espY2 overlap by 35 nucleotides
MCAODC_01240: leuC1 - 3-isopropylmalate dehydratase large subunit 1, at 231,739 to 233,139
leuC1
MCAODC_01245: leuD - 3-isopropylmalate dehydratase small subunit, at 233,150 to 233,755
leuD
MCAODC_01255: sgrR - DNA-binding transcriptional regulator SgrR, at 234,083 to 235,741
sgrR
MCAODC_01260: MCAODC_01260 - hypothetical protein, at 235,897 to 236,004
_01260
MCAODC_01265: espY2 - T3SS effector EspY2, at 235,970 to 236,539
espY2
Position (kb) 234
235
236 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 233.133 kb on + strand at 233.133 kb on + strand at 233.134 kb on - strand at 233.137 kb on + strand at 233.137 kb on + strand at 233.138 kb on - strand at 233.351 kb on + strand, within leuD at 233.443 kb on + strand, within leuD at 233.443 kb on + strand, within leuD at 233.443 kb on + strand, within leuD at 233.481 kb on - strand, within leuD at 233.488 kb on + strand, within leuD at 233.488 kb on + strand, within leuD at 233.489 kb on - strand, within leuD at 233.489 kb on - strand, within leuD at 233.521 kb on - strand, within leuD at 233.586 kb on + strand, within leuD at 233.587 kb on - strand, within leuD at 233.628 kb on - strand, within leuD at 233.692 kb on + strand, within leuD at 233.693 kb on - strand, within leuD at 233.747 kb on - strand at 233.747 kb on - strand at 233.753 kb on + strand at 233.754 kb on - strand at 233.760 kb on + strand at 233.805 kb on + strand at 233.805 kb on + strand at 233.806 kb on - strand at 233.828 kb on - strand at 233.836 kb on + strand at 233.836 kb on + strand at 233.837 kb on - strand at 233.914 kb on + strand at 233.972 kb on + strand at 233.972 kb on + strand at 233.973 kb on - strand at 233.973 kb on - strand at 234.019 kb on - strand at 234.019 kb on - strand at 234.025 kb on + strand at 234.033 kb on + strand at 234.033 kb on + strand at 234.034 kb on - strand at 234.036 kb on + strand at 234.036 kb on + strand at 234.039 kb on - strand at 234.039 kb on - strand at 234.139 kb on + strand at 234.139 kb on + strand at 234.140 kb on - strand at 234.177 kb on + strand at 234.200 kb on - strand at 234.200 kb on - strand at 234.268 kb on + strand, within sgrR at 234.269 kb on - strand, within sgrR at 234.293 kb on + strand, within sgrR at 234.293 kb on + strand, within sgrR at 234.294 kb on - strand, within sgrR at 234.294 kb on - strand, within sgrR at 234.361 kb on - strand, within sgrR at 234.459 kb on - strand, within sgrR at 234.461 kb on + strand, within sgrR at 234.461 kb on + strand, within sgrR at 234.475 kb on + strand, within sgrR at 234.476 kb on - strand, within sgrR at 234.552 kb on + strand, within sgrR at 234.552 kb on + strand, within sgrR at 234.606 kb on + strand, within sgrR at 234.606 kb on + strand, within sgrR at 234.606 kb on + strand, within sgrR at 234.710 kb on - strand, within sgrR at 234.722 kb on + strand, within sgrR at 234.730 kb on + strand, within sgrR at 234.804 kb on + strand, within sgrR at 234.805 kb on - strand, within sgrR at 234.859 kb on + strand, within sgrR at 234.859 kb on + strand, within sgrR at 234.980 kb on - strand, within sgrR at 235.032 kb on + strand, within sgrR at 235.032 kb on + strand, within sgrR at 235.032 kb on + strand, within sgrR at 235.033 kb on - strand, within sgrR at 235.033 kb on - strand, within sgrR at 235.088 kb on + strand, within sgrR at 235.088 kb on + strand, within sgrR at 235.088 kb on + strand, within sgrR at 235.091 kb on + strand, within sgrR at 235.091 kb on + strand, within sgrR at 235.092 kb on - strand, within sgrR at 235.095 kb on + strand, within sgrR at 235.096 kb on - strand, within sgrR at 235.096 kb on - strand, within sgrR at 235.096 kb on - strand, within sgrR at 235.149 kb on + strand, within sgrR at 235.154 kb on + strand, within sgrR at 235.154 kb on + strand, within sgrR at 235.154 kb on + strand, within sgrR at 235.154 kb on + strand, within sgrR at 235.154 kb on + strand, within sgrR at 235.154 kb on + strand, within sgrR at 235.155 kb on - strand, within sgrR at 235.155 kb on - strand, within sgrR at 235.155 kb on - strand, within sgrR at 235.171 kb on + strand, within sgrR at 235.171 kb on + strand, within sgrR at 235.171 kb on + strand, within sgrR at 235.172 kb on - strand, within sgrR at 235.181 kb on + strand, within sgrR at 235.181 kb on + strand, within sgrR at 235.199 kb on + strand, within sgrR at 235.400 kb on + strand, within sgrR at 235.400 kb on + strand, within sgrR at 235.400 kb on + strand, within sgrR at 235.436 kb on - strand, within sgrR at 235.444 kb on + strand, within sgrR at 235.445 kb on - strand, within sgrR at 235.503 kb on + strand, within sgrR at 235.503 kb on + strand, within sgrR at 235.523 kb on - strand, within sgrR at 235.523 kb on - strand, within sgrR at 235.627 kb on + strand at 235.627 kb on + strand at 235.663 kb on + strand at 235.740 kb on - strand at 235.740 kb on - strand at 235.777 kb on + strand at 235.793 kb on + strand at 235.794 kb on - strand at 235.799 kb on - strand at 235.799 kb on - strand at 235.803 kb on + strand at 235.804 kb on - strand at 235.810 kb on - strand at 235.826 kb on - strand at 235.918 kb on + strand, within MCAODC_01260 at 235.918 kb on + strand, within MCAODC_01260 at 235.978 kb on - strand at 236.058 kb on + strand, within espY2 at 236.059 kb on - strand, within espY2 at 236.065 kb on + strand, within espY2 at 236.066 kb on - strand, within espY2 at 236.066 kb on - strand, within espY2 at 236.119 kb on + strand, within espY2 at 236.119 kb on + strand, within espY2 at 236.120 kb on - strand, within espY2 at 236.120 kb on - strand, within espY2 at 236.120 kb on - strand, within espY2 at 236.157 kb on - strand, within espY2 at 236.162 kb on - strand, within espY2 at 236.253 kb on - strand, within espY2 at 236.253 kb on - strand, within espY2 at 236.257 kb on + strand, within espY2 at 236.302 kb on - strand, within espY2 at 236.302 kb on - strand, within espY2 at 236.322 kb on + strand, within espY2 at 236.369 kb on - strand, within espY2 at 236.403 kb on - strand, within espY2 at 236.403 kb on - strand, within espY2 at 236.470 kb on + strand, within espY2 at 236.471 kb on - strand, within espY2 at 236.471 kb on - strand, within espY2 at 236.473 kb on + strand, within espY2 at 236.489 kb on - strand at 236.494 kb on + strand at 236.497 kb on + strand at 236.509 kb on + strand at 236.509 kb on + strand at 236.513 kb on - strand at 236.513 kb on - strand at 236.546 kb on - strand at 236.560 kb on + strand at 236.561 kb on - strand at 236.587 kb on + strand at 236.594 kb on - strand at 236.629 kb on + strand at 236.635 kb on - strand at 236.638 kb on + strand at 236.638 kb on + strand at 236.685 kb on + strand at 236.693 kb on + strand at 236.705 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas14 remove 233,133 + +0.3 233,133 + +3.0 233,134 - -0.5 233,137 + +0.4 233,137 + +0.1 233,138 - -0.3 233,351 + leuD MCAODC_01245 0.33 -0.6 233,443 + leuD MCAODC_01245 0.48 +0.9 233,443 + leuD MCAODC_01245 0.48 -0.5 233,443 + leuD MCAODC_01245 0.48 +1.1 233,481 - leuD MCAODC_01245 0.55 +0.5 233,488 + leuD MCAODC_01245 0.56 -0.7 233,488 + leuD MCAODC_01245 0.56 -1.5 233,489 - leuD MCAODC_01245 0.56 +0.1 233,489 - leuD MCAODC_01245 0.56 -0.2 233,521 - leuD MCAODC_01245 0.61 -0.7 233,586 + leuD MCAODC_01245 0.72 -0.1 233,587 - leuD MCAODC_01245 0.72 -0.2 233,628 - leuD MCAODC_01245 0.79 -4.0 233,692 + leuD MCAODC_01245 0.89 +0.6 233,693 - leuD MCAODC_01245 0.90 -0.9 233,747 - +0.7 233,747 - -0.5 233,753 + -0.5 233,754 - +1.0 233,760 + -0.6 233,805 + -1.5 233,805 + +0.6 233,806 - -0.3 233,828 - +1.2 233,836 + -0.2 233,836 + -0.2 233,837 - +0.4 233,914 + +0.3 233,972 + -0.3 233,972 + -0.2 233,973 - +0.2 233,973 - +0.0 234,019 - +2.0 234,019 - -0.8 234,025 + +0.1 234,033 + -0.7 234,033 + +0.4 234,034 - -0.2 234,036 + -0.4 234,036 + -1.1 234,039 - -0.1 234,039 - -2.0 234,139 + +0.5 234,139 + -0.7 234,140 - -0.0 234,177 + -1.0 234,200 - -1.3 234,200 - +0.7 234,268 + sgrR MCAODC_01255 0.11 +0.6 234,269 - sgrR MCAODC_01255 0.11 -0.0 234,293 + sgrR MCAODC_01255 0.13 -0.7 234,293 + sgrR MCAODC_01255 0.13 -1.3 234,294 - sgrR MCAODC_01255 0.13 +0.5 234,294 - sgrR MCAODC_01255 0.13 +0.5 234,361 - sgrR MCAODC_01255 0.17 -1.1 234,459 - sgrR MCAODC_01255 0.23 +0.1 234,461 + sgrR MCAODC_01255 0.23 -0.8 234,461 + sgrR MCAODC_01255 0.23 -0.1 234,475 + sgrR MCAODC_01255 0.24 -0.4 234,476 - sgrR MCAODC_01255 0.24 +0.8 234,552 + sgrR MCAODC_01255 0.28 -0.2 234,552 + sgrR MCAODC_01255 0.28 -0.5 234,606 + sgrR MCAODC_01255 0.32 +0.0 234,606 + sgrR MCAODC_01255 0.32 -0.8 234,606 + sgrR MCAODC_01255 0.32 -0.5 234,710 - sgrR MCAODC_01255 0.38 +0.3 234,722 + sgrR MCAODC_01255 0.39 +0.2 234,730 + sgrR MCAODC_01255 0.39 +0.4 234,804 + sgrR MCAODC_01255 0.43 +0.7 234,805 - sgrR MCAODC_01255 0.44 +0.5 234,859 + sgrR MCAODC_01255 0.47 -0.1 234,859 + sgrR MCAODC_01255 0.47 +1.1 234,980 - sgrR MCAODC_01255 0.54 +0.3 235,032 + sgrR MCAODC_01255 0.57 -0.3 235,032 + sgrR MCAODC_01255 0.57 +0.3 235,032 + sgrR MCAODC_01255 0.57 +0.1 235,033 - sgrR MCAODC_01255 0.57 -1.6 235,033 - sgrR MCAODC_01255 0.57 +0.5 235,088 + sgrR MCAODC_01255 0.61 +0.5 235,088 + sgrR MCAODC_01255 0.61 -0.3 235,088 + sgrR MCAODC_01255 0.61 -0.6 235,091 + sgrR MCAODC_01255 0.61 +0.2 235,091 + sgrR MCAODC_01255 0.61 +0.3 235,092 - sgrR MCAODC_01255 0.61 +0.7 235,095 + sgrR MCAODC_01255 0.61 -0.1 235,096 - sgrR MCAODC_01255 0.61 -0.1 235,096 - sgrR MCAODC_01255 0.61 +0.6 235,096 - sgrR MCAODC_01255 0.61 +0.3 235,149 + sgrR MCAODC_01255 0.64 -0.3 235,154 + sgrR MCAODC_01255 0.65 +0.6 235,154 + sgrR MCAODC_01255 0.65 +0.4 235,154 + sgrR MCAODC_01255 0.65 +1.3 235,154 + sgrR MCAODC_01255 0.65 -0.7 235,154 + sgrR MCAODC_01255 0.65 +0.5 235,154 + sgrR MCAODC_01255 0.65 -0.2 235,155 - sgrR MCAODC_01255 0.65 -2.1 235,155 - sgrR MCAODC_01255 0.65 +0.3 235,155 - sgrR MCAODC_01255 0.65 -1.1 235,171 + sgrR MCAODC_01255 0.66 -0.1 235,171 + sgrR MCAODC_01255 0.66 +1.3 235,171 + sgrR MCAODC_01255 0.66 +0.3 235,172 - sgrR MCAODC_01255 0.66 +1.4 235,181 + sgrR MCAODC_01255 0.66 +1.1 235,181 + sgrR MCAODC_01255 0.66 +0.9 235,199 + sgrR MCAODC_01255 0.67 +0.0 235,400 + sgrR MCAODC_01255 0.79 -1.6 235,400 + sgrR MCAODC_01255 0.79 -0.5 235,400 + sgrR MCAODC_01255 0.79 -0.0 235,436 - sgrR MCAODC_01255 0.82 -0.7 235,444 + sgrR MCAODC_01255 0.82 -0.1 235,445 - sgrR MCAODC_01255 0.82 -0.7 235,503 + sgrR MCAODC_01255 0.86 +1.1 235,503 + sgrR MCAODC_01255 0.86 +0.7 235,523 - sgrR MCAODC_01255 0.87 +0.2 235,523 - sgrR MCAODC_01255 0.87 +0.7 235,627 + +0.9 235,627 + +1.2 235,663 + +0.5 235,740 - -0.7 235,740 - +0.4 235,777 + -0.0 235,793 + -2.5 235,794 - +0.3 235,799 - +0.2 235,799 - -0.1 235,803 + -1.3 235,804 - -0.6 235,810 - +0.6 235,826 - -2.0 235,918 + MCAODC_01260 0.19 -1.3 235,918 + MCAODC_01260 0.19 -1.1 235,978 - +0.7 236,058 + espY2 MCAODC_01265 0.15 -0.4 236,059 - espY2 MCAODC_01265 0.16 +0.2 236,065 + espY2 MCAODC_01265 0.17 +0.2 236,066 - espY2 MCAODC_01265 0.17 +2.4 236,066 - espY2 MCAODC_01265 0.17 +0.5 236,119 + espY2 MCAODC_01265 0.26 -0.0 236,119 + espY2 MCAODC_01265 0.26 +0.8 236,120 - espY2 MCAODC_01265 0.26 +0.6 236,120 - espY2 MCAODC_01265 0.26 +0.4 236,120 - espY2 MCAODC_01265 0.26 -0.8 236,157 - espY2 MCAODC_01265 0.33 +0.6 236,162 - espY2 MCAODC_01265 0.34 +0.3 236,253 - espY2 MCAODC_01265 0.50 +0.3 236,253 - espY2 MCAODC_01265 0.50 -0.7 236,257 + espY2 MCAODC_01265 0.50 +1.3 236,302 - espY2 MCAODC_01265 0.58 -0.2 236,302 - espY2 MCAODC_01265 0.58 +0.0 236,322 + espY2 MCAODC_01265 0.62 +0.5 236,369 - espY2 MCAODC_01265 0.70 -0.5 236,403 - espY2 MCAODC_01265 0.76 +1.0 236,403 - espY2 MCAODC_01265 0.76 +1.0 236,470 + espY2 MCAODC_01265 0.88 -0.5 236,471 - espY2 MCAODC_01265 0.88 +1.0 236,471 - espY2 MCAODC_01265 0.88 +0.3 236,473 + espY2 MCAODC_01265 0.88 +1.8 236,489 - -0.7 236,494 + -0.1 236,497 + +0.4 236,509 + -1.5 236,509 + +0.9 236,513 - +0.1 236,513 - +0.6 236,546 - +2.0 236,560 + +0.2 236,561 - -0.3 236,587 + +0.9 236,594 - +1.2 236,629 + -0.4 236,635 - +0.6 236,638 + +0.1 236,638 + +1.0 236,685 + +0.2 236,693 + +0.4 236,705 - -0.8
Or see this region's nucleotide sequence