Experiment: WM_Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcnB and folK overlap by 4 nucleotides folK and MCAODC_00880 are separated by 280 nucleotides MCAODC_00880 and ecpD are separated by 86 nucleotides ecpD and fimD are separated by 41 nucleotides
MCAODC_00870: pcnB - polynucleotide adenylyltransferase PcnB, at 153,795 to 155,159
pcnB
MCAODC_00875: folK - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, at 155,156 to 155,638
folK
MCAODC_00880: MCAODC_00880 - fimbrial protein, at 155,919 to 156,515
_00880
MCAODC_00885: ecpD - fimbrial chaperone, at 156,602 to 157,327
ecpD
MCAODC_00890: fimD - outer membrane usher protein, at 157,369 to 159,969
fimD
Position (kb) 155
156
157 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 154.946 kb on + strand, within pcnB at 154.946 kb on + strand, within pcnB at 154.962 kb on + strand, within pcnB at 154.997 kb on + strand, within pcnB at 155.168 kb on + strand at 155.168 kb on + strand at 155.168 kb on + strand at 155.586 kb on + strand, within folK at 155.613 kb on + strand at 155.614 kb on - strand at 155.616 kb on + strand at 155.617 kb on - strand at 155.659 kb on - strand at 155.664 kb on - strand at 155.683 kb on - strand at 155.868 kb on - strand at 155.896 kb on + strand at 155.977 kb on + strand at 155.977 kb on + strand at 155.978 kb on - strand at 155.978 kb on - strand at 156.010 kb on + strand, within MCAODC_00880 at 156.010 kb on + strand, within MCAODC_00880 at 156.026 kb on + strand, within MCAODC_00880 at 156.040 kb on + strand, within MCAODC_00880 at 156.040 kb on + strand, within MCAODC_00880 at 156.041 kb on - strand, within MCAODC_00880 at 156.041 kb on - strand, within MCAODC_00880 at 156.044 kb on + strand, within MCAODC_00880 at 156.044 kb on + strand, within MCAODC_00880 at 156.050 kb on + strand, within MCAODC_00880 at 156.051 kb on - strand, within MCAODC_00880 at 156.056 kb on + strand, within MCAODC_00880 at 156.057 kb on - strand, within MCAODC_00880 at 156.062 kb on + strand, within MCAODC_00880 at 156.089 kb on + strand, within MCAODC_00880 at 156.107 kb on + strand, within MCAODC_00880 at 156.122 kb on + strand, within MCAODC_00880 at 156.205 kb on + strand, within MCAODC_00880 at 156.205 kb on + strand, within MCAODC_00880 at 156.205 kb on + strand, within MCAODC_00880 at 156.205 kb on + strand, within MCAODC_00880 at 156.206 kb on - strand, within MCAODC_00880 at 156.209 kb on + strand, within MCAODC_00880 at 156.210 kb on - strand, within MCAODC_00880 at 156.210 kb on - strand, within MCAODC_00880 at 156.251 kb on + strand, within MCAODC_00880 at 156.257 kb on + strand, within MCAODC_00880 at 156.258 kb on - strand, within MCAODC_00880 at 156.258 kb on - strand, within MCAODC_00880 at 156.278 kb on + strand, within MCAODC_00880 at 156.278 kb on + strand, within MCAODC_00880 at 156.279 kb on - strand, within MCAODC_00880 at 156.314 kb on + strand, within MCAODC_00880 at 156.314 kb on + strand, within MCAODC_00880 at 156.425 kb on + strand, within MCAODC_00880 at 156.435 kb on + strand, within MCAODC_00880 at 156.435 kb on + strand, within MCAODC_00880 at 156.436 kb on - strand, within MCAODC_00880 at 156.478 kb on + strand at 156.486 kb on - strand at 156.507 kb on + strand at 156.507 kb on + strand at 156.507 kb on + strand at 156.507 kb on + strand at 156.508 kb on - strand at 156.513 kb on + strand at 156.601 kb on + strand at 156.683 kb on - strand, within ecpD at 156.701 kb on - strand, within ecpD at 156.708 kb on - strand, within ecpD at 156.893 kb on + strand, within ecpD at 156.893 kb on + strand, within ecpD at 156.893 kb on + strand, within ecpD at 156.893 kb on + strand, within ecpD at 156.893 kb on + strand, within ecpD at 156.893 kb on + strand, within ecpD at 156.893 kb on + strand, within ecpD at 156.893 kb on + strand, within ecpD at 156.893 kb on + strand, within ecpD at 156.894 kb on - strand, within ecpD at 156.894 kb on - strand, within ecpD at 156.935 kb on + strand, within ecpD at 157.000 kb on - strand, within ecpD at 157.110 kb on + strand, within ecpD at 157.111 kb on - strand, within ecpD at 157.121 kb on - strand, within ecpD at 157.121 kb on - strand, within ecpD at 157.133 kb on + strand, within ecpD at 157.134 kb on - strand, within ecpD at 157.136 kb on + strand, within ecpD at 157.136 kb on + strand, within ecpD at 157.136 kb on + strand, within ecpD at 157.136 kb on + strand, within ecpD at 157.136 kb on + strand, within ecpD at 157.136 kb on + strand, within ecpD at 157.136 kb on + strand, within ecpD at 157.137 kb on - strand, within ecpD at 157.137 kb on - strand, within ecpD at 157.137 kb on - strand, within ecpD at 157.137 kb on - strand, within ecpD at 157.137 kb on - strand, within ecpD at 157.147 kb on + strand, within ecpD at 157.181 kb on + strand, within ecpD at 157.182 kb on - strand, within ecpD at 157.277 kb on - strand at 157.347 kb on + strand at 157.380 kb on + strand at 157.380 kb on + strand at 157.381 kb on - strand at 157.441 kb on + strand at 157.443 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas14 remove 154,946 + pcnB MCAODC_00870 0.84 -2.0 154,946 + pcnB MCAODC_00870 0.84 -0.7 154,962 + pcnB MCAODC_00870 0.85 +0.4 154,997 + pcnB MCAODC_00870 0.88 -0.8 155,168 + -0.5 155,168 + -1.3 155,168 + -1.4 155,586 + folK MCAODC_00875 0.89 -1.7 155,613 + -1.6 155,614 - +0.0 155,616 + +0.9 155,617 - +1.5 155,659 - +0.2 155,664 - +0.9 155,683 - +0.1 155,868 - +1.4 155,896 + -0.2 155,977 + +0.5 155,977 + +0.6 155,978 - +0.5 155,978 - +0.7 156,010 + MCAODC_00880 0.15 -0.2 156,010 + MCAODC_00880 0.15 +0.0 156,026 + MCAODC_00880 0.18 +0.4 156,040 + MCAODC_00880 0.20 +0.9 156,040 + MCAODC_00880 0.20 +2.0 156,041 - MCAODC_00880 0.20 -0.7 156,041 - MCAODC_00880 0.20 -0.2 156,044 + MCAODC_00880 0.21 -1.1 156,044 + MCAODC_00880 0.21 +0.8 156,050 + MCAODC_00880 0.22 +0.0 156,051 - MCAODC_00880 0.22 -1.8 156,056 + MCAODC_00880 0.23 -0.1 156,057 - MCAODC_00880 0.23 +2.0 156,062 + MCAODC_00880 0.24 -0.5 156,089 + MCAODC_00880 0.28 -0.1 156,107 + MCAODC_00880 0.31 +1.1 156,122 + MCAODC_00880 0.34 +0.2 156,205 + MCAODC_00880 0.48 -0.9 156,205 + MCAODC_00880 0.48 +0.7 156,205 + MCAODC_00880 0.48 -1.3 156,205 + MCAODC_00880 0.48 -0.1 156,206 - MCAODC_00880 0.48 -0.6 156,209 + MCAODC_00880 0.49 +0.3 156,210 - MCAODC_00880 0.49 -0.6 156,210 - MCAODC_00880 0.49 -0.3 156,251 + MCAODC_00880 0.56 +0.5 156,257 + MCAODC_00880 0.57 -0.3 156,258 - MCAODC_00880 0.57 +0.3 156,258 - MCAODC_00880 0.57 +0.0 156,278 + MCAODC_00880 0.60 +0.0 156,278 + MCAODC_00880 0.60 +0.5 156,279 - MCAODC_00880 0.60 +0.4 156,314 + MCAODC_00880 0.66 +1.6 156,314 + MCAODC_00880 0.66 +0.7 156,425 + MCAODC_00880 0.85 -1.0 156,435 + MCAODC_00880 0.86 +0.8 156,435 + MCAODC_00880 0.86 -0.8 156,436 - MCAODC_00880 0.87 +0.2 156,478 + +0.1 156,486 - -0.8 156,507 + -2.2 156,507 + +0.5 156,507 + +0.4 156,507 + -0.2 156,508 - +0.9 156,513 + +2.0 156,601 + -0.9 156,683 - ecpD MCAODC_00885 0.11 +1.1 156,701 - ecpD MCAODC_00885 0.14 -1.8 156,708 - ecpD MCAODC_00885 0.15 +1.7 156,893 + ecpD MCAODC_00885 0.40 -2.4 156,893 + ecpD MCAODC_00885 0.40 +0.3 156,893 + ecpD MCAODC_00885 0.40 +0.1 156,893 + ecpD MCAODC_00885 0.40 +0.5 156,893 + ecpD MCAODC_00885 0.40 -1.2 156,893 + ecpD MCAODC_00885 0.40 +0.1 156,893 + ecpD MCAODC_00885 0.40 +0.0 156,893 + ecpD MCAODC_00885 0.40 +1.1 156,893 + ecpD MCAODC_00885 0.40 -3.2 156,894 - ecpD MCAODC_00885 0.40 -0.5 156,894 - ecpD MCAODC_00885 0.40 -0.5 156,935 + ecpD MCAODC_00885 0.46 +1.3 157,000 - ecpD MCAODC_00885 0.55 -0.3 157,110 + ecpD MCAODC_00885 0.70 -1.6 157,111 - ecpD MCAODC_00885 0.70 +0.4 157,121 - ecpD MCAODC_00885 0.71 +0.7 157,121 - ecpD MCAODC_00885 0.71 +0.5 157,133 + ecpD MCAODC_00885 0.73 -0.1 157,134 - ecpD MCAODC_00885 0.73 -1.0 157,136 + ecpD MCAODC_00885 0.74 +1.2 157,136 + ecpD MCAODC_00885 0.74 -0.6 157,136 + ecpD MCAODC_00885 0.74 +0.4 157,136 + ecpD MCAODC_00885 0.74 -0.5 157,136 + ecpD MCAODC_00885 0.74 +0.6 157,136 + ecpD MCAODC_00885 0.74 -0.0 157,136 + ecpD MCAODC_00885 0.74 -0.7 157,137 - ecpD MCAODC_00885 0.74 +0.5 157,137 - ecpD MCAODC_00885 0.74 +0.5 157,137 - ecpD MCAODC_00885 0.74 +0.5 157,137 - ecpD MCAODC_00885 0.74 +1.5 157,137 - ecpD MCAODC_00885 0.74 -0.3 157,147 + ecpD MCAODC_00885 0.75 +0.2 157,181 + ecpD MCAODC_00885 0.80 -0.0 157,182 - ecpD MCAODC_00885 0.80 +0.4 157,277 - +0.1 157,347 + +0.3 157,380 + +0.4 157,380 + +0.7 157,381 - -1.3 157,441 + +0.8 157,443 + +0.4
Or see this region's nucleotide sequence