Experiment: Thymine 0.793 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS00115 and A4249_RS16710 are separated by 4 nucleotides A4249_RS16710 and A4249_RS00120 overlap by 4 nucleotides A4249_RS00120 and A4249_RS00125 overlap by 4 nucleotides
A4249_RS00115: A4249_RS00115 - heme o synthase, at 19,924 to 20,889
_RS00115
A4249_RS16710: A4249_RS16710 - hypothetical protein, at 20,894 to 21,064
_RS16710
A4249_RS00120: A4249_RS00120 - cytochrome c oxidase assembly protein, at 21,061 to 21,597
_RS00120
A4249_RS00125: A4249_RS00125 - NUDIX domain-containing protein, at 21,594 to 22,010
_RS00125
Position (kb) 20
21
22 Strain fitness (log2 ratio) -1
0
1
2 at 19.908 kb on + strand at 19.908 kb on + strand at 19.908 kb on + strand at 19.908 kb on + strand at 19.908 kb on + strand at 19.908 kb on + strand at 19.908 kb on + strand at 19.908 kb on + strand at 19.908 kb on + strand at 19.908 kb on + strand at 19.908 kb on + strand at 19.908 kb on + strand at 19.908 kb on + strand at 19.909 kb on - strand at 19.909 kb on - strand at 19.909 kb on - strand at 19.909 kb on - strand at 20.311 kb on + strand, within A4249_RS00115 at 20.312 kb on - strand, within A4249_RS00115 at 20.326 kb on + strand, within A4249_RS00115 at 20.326 kb on + strand, within A4249_RS00115 at 20.503 kb on + strand, within A4249_RS00115 at 20.591 kb on - strand, within A4249_RS00115 at 20.773 kb on + strand, within A4249_RS00115 at 20.773 kb on + strand, within A4249_RS00115 at 20.773 kb on + strand, within A4249_RS00115 at 20.773 kb on + strand, within A4249_RS00115 at 20.773 kb on + strand, within A4249_RS00115 at 20.773 kb on + strand, within A4249_RS00115 at 20.773 kb on + strand, within A4249_RS00115 at 20.774 kb on - strand, within A4249_RS00115 at 20.887 kb on + strand at 20.887 kb on + strand at 20.887 kb on + strand at 20.888 kb on - strand at 20.888 kb on - strand at 20.970 kb on + strand, within A4249_RS16710 at 20.970 kb on + strand, within A4249_RS16710 at 20.970 kb on + strand, within A4249_RS16710 at 20.971 kb on - strand, within A4249_RS16710 at 20.971 kb on - strand, within A4249_RS16710 at 20.971 kb on - strand, within A4249_RS16710 at 20.971 kb on - strand, within A4249_RS16710 at 21.334 kb on + strand, within A4249_RS00120 at 21.335 kb on - strand, within A4249_RS00120 at 21.385 kb on + strand, within A4249_RS00120 at 21.385 kb on + strand, within A4249_RS00120 at 21.385 kb on + strand, within A4249_RS00120 at 21.385 kb on + strand, within A4249_RS00120 at 21.385 kb on + strand, within A4249_RS00120 at 21.385 kb on + strand, within A4249_RS00120 at 21.385 kb on + strand, within A4249_RS00120 at 21.386 kb on - strand, within A4249_RS00120 at 21.386 kb on - strand, within A4249_RS00120 at 21.386 kb on - strand, within A4249_RS00120 at 21.386 kb on - strand, within A4249_RS00120 at 21.386 kb on - strand, within A4249_RS00120 at 21.386 kb on - strand, within A4249_RS00120 at 21.386 kb on - strand, within A4249_RS00120 at 21.386 kb on - strand, within A4249_RS00120 at 21.415 kb on + strand, within A4249_RS00120 at 21.415 kb on + strand, within A4249_RS00120 at 21.416 kb on - strand, within A4249_RS00120 at 21.493 kb on + strand, within A4249_RS00120 at 21.494 kb on - strand, within A4249_RS00120 at 21.494 kb on - strand, within A4249_RS00120 at 21.559 kb on + strand at 21.559 kb on + strand at 21.559 kb on + strand at 21.559 kb on + strand at 21.560 kb on - strand at 21.560 kb on - strand at 21.560 kb on - strand at 21.568 kb on + strand at 21.568 kb on + strand at 21.568 kb on + strand at 21.568 kb on + strand at 21.568 kb on + strand at 21.569 kb on - strand at 21.569 kb on - strand at 21.569 kb on - strand at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.870 kb on + strand, within A4249_RS00125 at 21.871 kb on - strand, within A4249_RS00125 at 21.871 kb on - strand, within A4249_RS00125 at 21.871 kb on - strand, within A4249_RS00125 at 21.871 kb on - strand, within A4249_RS00125 at 21.871 kb on - strand, within A4249_RS00125 at 21.871 kb on - strand, within A4249_RS00125 at 21.871 kb on - strand, within A4249_RS00125 at 21.871 kb on - strand, within A4249_RS00125 at 21.871 kb on - strand, within A4249_RS00125 at 21.871 kb on - strand, within A4249_RS00125
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine 0.793 mM (C) remove 19,908 + +0.7 19,908 + +0.5 19,908 + +0.0 19,908 + +0.4 19,908 + +0.0 19,908 + -0.0 19,908 + -0.3 19,908 + +0.7 19,908 + +0.8 19,908 + +0.8 19,908 + +0.1 19,908 + +0.1 19,908 + +0.7 19,909 - -0.0 19,909 - +0.3 19,909 - +0.7 19,909 - +0.4 20,311 + A4249_RS00115 0.40 +1.6 20,312 - A4249_RS00115 0.40 +0.3 20,326 + A4249_RS00115 0.42 +1.6 20,326 + A4249_RS00115 0.42 -0.7 20,503 + A4249_RS00115 0.60 +1.6 20,591 - A4249_RS00115 0.69 -0.5 20,773 + A4249_RS00115 0.88 +0.4 20,773 + A4249_RS00115 0.88 +0.8 20,773 + A4249_RS00115 0.88 +0.4 20,773 + A4249_RS00115 0.88 +1.1 20,773 + A4249_RS00115 0.88 +1.6 20,773 + A4249_RS00115 0.88 -0.7 20,773 + A4249_RS00115 0.88 +0.2 20,774 - A4249_RS00115 0.88 -0.4 20,887 + -0.9 20,887 + +1.5 20,887 + +0.5 20,888 - +0.4 20,888 - +1.6 20,970 + A4249_RS16710 0.44 +1.6 20,970 + A4249_RS16710 0.44 +0.1 20,970 + A4249_RS16710 0.44 +0.0 20,971 - A4249_RS16710 0.45 -0.3 20,971 - A4249_RS16710 0.45 -0.9 20,971 - A4249_RS16710 0.45 +2.0 20,971 - A4249_RS16710 0.45 -0.3 21,334 + A4249_RS00120 0.51 +1.1 21,335 - A4249_RS00120 0.51 +0.1 21,385 + A4249_RS00120 0.60 -0.4 21,385 + A4249_RS00120 0.60 +1.1 21,385 + A4249_RS00120 0.60 +0.3 21,385 + A4249_RS00120 0.60 -0.0 21,385 + A4249_RS00120 0.60 +0.3 21,385 + A4249_RS00120 0.60 +0.3 21,385 + A4249_RS00120 0.60 +0.6 21,386 - A4249_RS00120 0.61 -0.5 21,386 - A4249_RS00120 0.61 +0.4 21,386 - A4249_RS00120 0.61 -0.3 21,386 - A4249_RS00120 0.61 +0.9 21,386 - A4249_RS00120 0.61 +0.5 21,386 - A4249_RS00120 0.61 +2.0 21,386 - A4249_RS00120 0.61 -0.4 21,386 - A4249_RS00120 0.61 +0.3 21,415 + A4249_RS00120 0.66 +0.4 21,415 + A4249_RS00120 0.66 +0.5 21,416 - A4249_RS00120 0.66 -0.6 21,493 + A4249_RS00120 0.80 +1.4 21,494 - A4249_RS00120 0.81 +0.8 21,494 - A4249_RS00120 0.81 +0.5 21,559 + +1.9 21,559 + +0.6 21,559 + -0.1 21,559 + +0.5 21,560 - -1.3 21,560 - +0.6 21,560 - +0.0 21,568 + -0.1 21,568 + +0.3 21,568 + +0.8 21,568 + +0.3 21,568 + +0.9 21,569 - +0.3 21,569 - -0.7 21,569 - -0.3 21,870 + A4249_RS00125 0.66 -0.7 21,870 + A4249_RS00125 0.66 +0.0 21,870 + A4249_RS00125 0.66 -0.1 21,870 + A4249_RS00125 0.66 -0.0 21,870 + A4249_RS00125 0.66 +0.3 21,870 + A4249_RS00125 0.66 -1.7 21,870 + A4249_RS00125 0.66 +0.2 21,870 + A4249_RS00125 0.66 -0.4 21,870 + A4249_RS00125 0.66 -0.5 21,870 + A4249_RS00125 0.66 +0.3 21,870 + A4249_RS00125 0.66 +0.6 21,870 + A4249_RS00125 0.66 +0.3 21,870 + A4249_RS00125 0.66 -0.3 21,871 - A4249_RS00125 0.66 +0.4 21,871 - A4249_RS00125 0.66 +0.5 21,871 - A4249_RS00125 0.66 +0.7 21,871 - A4249_RS00125 0.66 +0.9 21,871 - A4249_RS00125 0.66 +0.5 21,871 - A4249_RS00125 0.66 +0.4 21,871 - A4249_RS00125 0.66 +0.6 21,871 - A4249_RS00125 0.66 +0.4 21,871 - A4249_RS00125 0.66 -0.7 21,871 - A4249_RS00125 0.66 -0.7
Or see this region's nucleotide sequence