Strain Fitness in Azospirillum sp. SherDot2 around MPMX19_06602

Experiment: NF_with_fullN

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMPMX19_06600 and MPMX19_06601 overlap by 4 nucleotidesMPMX19_06601 and MPMX19_06602 are separated by 8 nucleotidesMPMX19_06602 and MPMX19_06603 are separated by 56 nucleotides MPMX19_06600: MPMX19_06600 - hypothetical protein, at 95,334 to 97,496 _06600 MPMX19_06601: MPMX19_06601 - hypothetical protein, at 97,493 to 98,329 _06601 MPMX19_06602: MPMX19_06602 - hypothetical protein, at 98,338 to 99,819 _06602 MPMX19_06603: MPMX19_06603 - hypothetical protein, at 99,876 to 102,161 _06603 Position (kb) 98 99 100Strain fitness (log2 ratio) -2 -1 0 1 2at 97.409 kb on + strandat 97.409 kb on + strandat 97.409 kb on + strandat 97.409 kb on + strandat 97.409 kb on + strandat 97.409 kb on + strandat 97.410 kb on - strandat 97.410 kb on - strandat 97.410 kb on - strandat 97.410 kb on - strandat 97.410 kb on - strandat 97.410 kb on - strandat 97.410 kb on - strandat 97.410 kb on - strandat 97.410 kb on - strandat 97.410 kb on - strandat 97.410 kb on - strandat 97.433 kb on + strandat 97.434 kb on - strandat 97.434 kb on - strandat 97.745 kb on + strand, within MPMX19_06601at 97.745 kb on + strand, within MPMX19_06601at 97.746 kb on - strand, within MPMX19_06601at 97.746 kb on - strand, within MPMX19_06601at 97.746 kb on - strand, within MPMX19_06601at 97.746 kb on - strand, within MPMX19_06601at 97.746 kb on - strand, within MPMX19_06601at 97.746 kb on - strand, within MPMX19_06601at 97.746 kb on - strand, within MPMX19_06601at 97.746 kb on - strand, within MPMX19_06601at 97.746 kb on - strand, within MPMX19_06601at 98.500 kb on + strand, within MPMX19_06602at 98.501 kb on - strand, within MPMX19_06602at 98.501 kb on - strand, within MPMX19_06602at 98.501 kb on - strand, within MPMX19_06602at 98.501 kb on - strand, within MPMX19_06602at 98.953 kb on + strand, within MPMX19_06602at 98.953 kb on + strand, within MPMX19_06602at 98.953 kb on + strand, within MPMX19_06602at 98.953 kb on + strand, within MPMX19_06602at 98.953 kb on + strand, within MPMX19_06602at 98.954 kb on - strand, within MPMX19_06602at 99.634 kb on + strand, within MPMX19_06602at 99.634 kb on + strand, within MPMX19_06602at 99.634 kb on + strand, within MPMX19_06602at 99.634 kb on + strand, within MPMX19_06602at 99.634 kb on + strand, within MPMX19_06602at 99.634 kb on + strand, within MPMX19_06602at 99.634 kb on + strand, within MPMX19_06602at 99.634 kb on + strand, within MPMX19_06602at 99.635 kb on - strand, within MPMX19_06602at 99.635 kb on - strand, within MPMX19_06602at 99.635 kb on - strand, within MPMX19_06602at 99.635 kb on - strand, within MPMX19_06602at 99.635 kb on - strand, within MPMX19_06602at 99.635 kb on - strand, within MPMX19_06602at 99.635 kb on - strand, within MPMX19_06602at 99.635 kb on - strand, within MPMX19_06602at 99.671 kb on - strandat 99.769 kb on + strandat 99.769 kb on + strandat 99.769 kb on + strandat 99.769 kb on + strandat 99.769 kb on + strandat 99.769 kb on + strandat 99.769 kb on + strandat 99.769 kb on + strandat 99.769 kb on + strandat 99.769 kb on + strandat 99.770 kb on - strandat 99.770 kb on - strandat 99.770 kb on - strandat 99.770 kb on - strandat 99.770 kb on - strandat 99.770 kb on - strandat 99.770 kb on - strandat 99.770 kb on - strandat 99.770 kb on - strandat 99.770 kb on - strandat 100.071 kb on + strandat 100.071 kb on + strandat 100.071 kb on + strandat 100.071 kb on + strandat 100.072 kb on - strandat 100.072 kb on - strandat 100.072 kb on - strandat 100.072 kb on - strandat 100.072 kb on - strandat 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.200 kb on + strand, within MPMX19_06603at 100.201 kb on - strand, within MPMX19_06603at 100.201 kb on - strand, within MPMX19_06603at 100.201 kb on - strand, within MPMX19_06603at 100.201 kb on - strand, within MPMX19_06603at 100.201 kb on - strand, within MPMX19_06603at 100.201 kb on - strand, within MPMX19_06603at 100.201 kb on - strand, within MPMX19_06603at 100.201 kb on - strand, within MPMX19_06603at 100.201 kb on - strand, within MPMX19_06603at 100.201 kb on - strand, within MPMX19_06603at 100.201 kb on - strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.308 kb on + strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.309 kb on - strand, within MPMX19_06603at 100.351 kb on + strand, within MPMX19_06603at 100.351 kb on + strand, within MPMX19_06603at 100.351 kb on + strand, within MPMX19_06603at 100.352 kb on - strand, within MPMX19_06603at 100.383 kb on + strand, within MPMX19_06603at 100.383 kb on + strand, within MPMX19_06603at 100.383 kb on + strand, within MPMX19_06603at 100.383 kb on + strand, within MPMX19_06603at 100.383 kb on + strand, within MPMX19_06603at 100.383 kb on + strand, within MPMX19_06603at 100.383 kb on + strand, within MPMX19_06603at 100.383 kb on + strand, within MPMX19_06603at 100.383 kb on + strand, within MPMX19_06603at 100.384 kb on - strand, within MPMX19_06603at 100.384 kb on - strand, within MPMX19_06603at 100.384 kb on - strand, within MPMX19_06603at 100.384 kb on - strand, within MPMX19_06603at 100.384 kb on - strand, within MPMX19_06603at 100.384 kb on - strand, within MPMX19_06603at 100.509 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.560 kb on + strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.561 kb on - strand, within MPMX19_06603at 100.632 kb on + strand, within MPMX19_06603at 100.632 kb on + strand, within MPMX19_06603at 100.632 kb on + strand, within MPMX19_06603at 100.632 kb on + strand, within MPMX19_06603at 100.632 kb on + strand, within MPMX19_06603at 100.632 kb on + strand, within MPMX19_06603at 100.632 kb on + strand, within MPMX19_06603at 100.632 kb on + strand, within MPMX19_06603at 100.633 kb on - strand, within MPMX19_06603at 100.633 kb on - strand, within MPMX19_06603at 100.633 kb on - strand, within MPMX19_06603at 100.633 kb on - strand, within MPMX19_06603at 100.633 kb on - strand, within MPMX19_06603at 100.633 kb on - strand, within MPMX19_06603at 100.633 kb on - strand, within MPMX19_06603at 100.633 kb on - strand, within MPMX19_06603at 100.633 kb on - strand, within MPMX19_06603at 100.633 kb on - strand, within MPMX19_06603at 100.633 kb on - strand, within MPMX19_06603at 100.782 kb on + strand, within MPMX19_06603at 100.782 kb on + strand, within MPMX19_06603at 100.782 kb on + strand, within MPMX19_06603at 100.782 kb on + strand, within MPMX19_06603at 100.782 kb on + strand, within MPMX19_06603at 100.782 kb on + strand, within MPMX19_06603at 100.782 kb on + strand, within MPMX19_06603at 100.783 kb on - strand, within MPMX19_06603

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Per-strain Table

Position Strand Gene LocusTag Fraction NF_with_fullN
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97,409 + -0.8
97,409 + -0.5
97,409 + -0.4
97,409 + +2.4
97,409 + -0.3
97,409 + +0.2
97,410 - -1.8
97,410 - -0.4
97,410 - -1.0
97,410 - -1.2
97,410 - +0.8
97,410 - -1.2
97,410 - -0.9
97,410 - -0.8
97,410 - +0.8
97,410 - -2.0
97,410 - +0.4
97,433 + -0.4
97,434 - -0.3
97,434 - +0.0
97,745 + MPMX19_06601 0.30 +0.8
97,745 + MPMX19_06601 0.30 +0.5
97,746 - MPMX19_06601 0.30 -0.2
97,746 - MPMX19_06601 0.30 -0.8
97,746 - MPMX19_06601 0.30 +0.4
97,746 - MPMX19_06601 0.30 -1.5
97,746 - MPMX19_06601 0.30 -1.2
97,746 - MPMX19_06601 0.30 +0.6
97,746 - MPMX19_06601 0.30 +0.7
97,746 - MPMX19_06601 0.30 -2.0
97,746 - MPMX19_06601 0.30 +0.5
98,500 + MPMX19_06602 0.11 +0.2
98,501 - MPMX19_06602 0.11 -1.2
98,501 - MPMX19_06602 0.11 -0.2
98,501 - MPMX19_06602 0.11 -0.5
98,501 - MPMX19_06602 0.11 -1.2
98,953 + MPMX19_06602 0.41 +1.2
98,953 + MPMX19_06602 0.41 -1.4
98,953 + MPMX19_06602 0.41 +0.7
98,953 + MPMX19_06602 0.41 -0.5
98,953 + MPMX19_06602 0.41 -1.5
98,954 - MPMX19_06602 0.42 +0.8
99,634 + MPMX19_06602 0.87 +0.2
99,634 + MPMX19_06602 0.87 +0.4
99,634 + MPMX19_06602 0.87 +1.2
99,634 + MPMX19_06602 0.87 -0.5
99,634 + MPMX19_06602 0.87 -0.2
99,634 + MPMX19_06602 0.87 -0.4
99,634 + MPMX19_06602 0.87 +1.0
99,634 + MPMX19_06602 0.87 -0.9
99,635 - MPMX19_06602 0.88 -0.3
99,635 - MPMX19_06602 0.88 +0.0
99,635 - MPMX19_06602 0.88 -0.8
99,635 - MPMX19_06602 0.88 +0.1
99,635 - MPMX19_06602 0.88 +1.2
99,635 - MPMX19_06602 0.88 -1.8
99,635 - MPMX19_06602 0.88 +0.6
99,635 - MPMX19_06602 0.88 +1.2
99,671 - -0.0
99,769 + -0.5
99,769 + +0.1
99,769 + -0.5
99,769 + +0.4
99,769 + -0.9
99,769 + -0.2
99,769 + +1.6
99,769 + -0.1
99,769 + -2.2
99,769 + -0.9
99,770 - +2.2
99,770 - -0.4
99,770 - -0.8
99,770 - +0.6
99,770 - +0.2
99,770 - -1.2
99,770 - -1.2
99,770 - +0.5
99,770 - +0.5
99,770 - +0.5
100,071 + -0.2
100,071 + -2.6
100,071 + -0.9
100,071 + +1.2
100,072 - -1.5
100,072 - -0.5
100,072 - +1.6
100,072 - -0.8
100,072 - +1.4
100,200 + MPMX19_06603 0.14 -1.2
100,200 + MPMX19_06603 0.14 -0.2
100,200 + MPMX19_06603 0.14 -0.3
100,200 + MPMX19_06603 0.14 -1.2
100,200 + MPMX19_06603 0.14 +0.5
100,200 + MPMX19_06603 0.14 -0.3
100,200 + MPMX19_06603 0.14 -0.6
100,200 + MPMX19_06603 0.14 -0.1
100,200 + MPMX19_06603 0.14 +0.8
100,200 + MPMX19_06603 0.14 +0.8
100,200 + MPMX19_06603 0.14 +1.3
100,200 + MPMX19_06603 0.14 +0.8
100,200 + MPMX19_06603 0.14 +0.8
100,200 + MPMX19_06603 0.14 -0.0
100,200 + MPMX19_06603 0.14 -0.2
100,200 + MPMX19_06603 0.14 +0.4
100,200 + MPMX19_06603 0.14 +0.4
100,200 + MPMX19_06603 0.14 +0.6
100,201 - MPMX19_06603 0.14 +0.4
100,201 - MPMX19_06603 0.14 -0.8
100,201 - MPMX19_06603 0.14 +0.5
100,201 - MPMX19_06603 0.14 -0.2
100,201 - MPMX19_06603 0.14 -0.6
100,201 - MPMX19_06603 0.14 -0.0
100,201 - MPMX19_06603 0.14 +0.3
100,201 - MPMX19_06603 0.14 -0.8
100,201 - MPMX19_06603 0.14 -0.6
100,201 - MPMX19_06603 0.14 -1.2
100,201 - MPMX19_06603 0.14 -0.8
100,308 + MPMX19_06603 0.19 -0.2
100,308 + MPMX19_06603 0.19 -0.4
100,308 + MPMX19_06603 0.19 +0.8
100,308 + MPMX19_06603 0.19 +1.1
100,308 + MPMX19_06603 0.19 +0.1
100,308 + MPMX19_06603 0.19 -1.2
100,308 + MPMX19_06603 0.19 -1.6
100,308 + MPMX19_06603 0.19 -0.8
100,308 + MPMX19_06603 0.19 -1.3
100,308 + MPMX19_06603 0.19 -0.5
100,308 + MPMX19_06603 0.19 -1.1
100,308 + MPMX19_06603 0.19 -0.8
100,308 + MPMX19_06603 0.19 -0.3
100,308 + MPMX19_06603 0.19 -1.5
100,308 + MPMX19_06603 0.19 +1.4
100,308 + MPMX19_06603 0.19 -0.5
100,308 + MPMX19_06603 0.19 -1.2
100,308 + MPMX19_06603 0.19 +1.3
100,308 + MPMX19_06603 0.19 -0.2
100,308 + MPMX19_06603 0.19 +1.6
100,309 - MPMX19_06603 0.19 -1.5
100,309 - MPMX19_06603 0.19 -0.2
100,309 - MPMX19_06603 0.19 -1.3
100,309 - MPMX19_06603 0.19 -0.2
100,309 - MPMX19_06603 0.19 +0.8
100,309 - MPMX19_06603 0.19 -1.1
100,309 - MPMX19_06603 0.19 -0.9
100,309 - MPMX19_06603 0.19 +1.0
100,309 - MPMX19_06603 0.19 -1.5
100,309 - MPMX19_06603 0.19 +1.0
100,309 - MPMX19_06603 0.19 -0.9
100,309 - MPMX19_06603 0.19 -0.4
100,309 - MPMX19_06603 0.19 -0.2
100,309 - MPMX19_06603 0.19 -0.8
100,351 + MPMX19_06603 0.21 -0.6
100,351 + MPMX19_06603 0.21 +0.4
100,351 + MPMX19_06603 0.21 +0.2
100,352 - MPMX19_06603 0.21 -1.5
100,383 + MPMX19_06603 0.22 +0.2
100,383 + MPMX19_06603 0.22 -0.2
100,383 + MPMX19_06603 0.22 +0.2
100,383 + MPMX19_06603 0.22 +0.8
100,383 + MPMX19_06603 0.22 -0.4
100,383 + MPMX19_06603 0.22 -1.5
100,383 + MPMX19_06603 0.22 -0.5
100,383 + MPMX19_06603 0.22 +0.2
100,383 + MPMX19_06603 0.22 -1.6
100,384 - MPMX19_06603 0.22 -0.8
100,384 - MPMX19_06603 0.22 -1.5
100,384 - MPMX19_06603 0.22 -1.0
100,384 - MPMX19_06603 0.22 -0.4
100,384 - MPMX19_06603 0.22 -0.8
100,384 - MPMX19_06603 0.22 +0.8
100,509 + MPMX19_06603 0.28 -1.0
100,560 + MPMX19_06603 0.30 +0.8
100,560 + MPMX19_06603 0.30 +0.0
100,560 + MPMX19_06603 0.30 +0.2
100,560 + MPMX19_06603 0.30 -0.2
100,560 + MPMX19_06603 0.30 +0.2
100,560 + MPMX19_06603 0.30 +0.4
100,560 + MPMX19_06603 0.30 -0.8
100,560 + MPMX19_06603 0.30 +0.1
100,560 + MPMX19_06603 0.30 -0.0
100,560 + MPMX19_06603 0.30 -0.2
100,560 + MPMX19_06603 0.30 -1.0
100,560 + MPMX19_06603 0.30 -2.2
100,560 + MPMX19_06603 0.30 -0.2
100,560 + MPMX19_06603 0.30 +0.8
100,560 + MPMX19_06603 0.30 +0.2
100,560 + MPMX19_06603 0.30 -0.8
100,560 + MPMX19_06603 0.30 -0.5
100,560 + MPMX19_06603 0.30 -0.1
100,560 + MPMX19_06603 0.30 -1.5
100,560 + MPMX19_06603 0.30 -0.4
100,560 + MPMX19_06603 0.30 -1.3
100,561 - MPMX19_06603 0.30 -0.8
100,561 - MPMX19_06603 0.30 +0.8
100,561 - MPMX19_06603 0.30 +0.6
100,561 - MPMX19_06603 0.30 +0.8
100,561 - MPMX19_06603 0.30 -1.5
100,561 - MPMX19_06603 0.30 +0.6
100,561 - MPMX19_06603 0.30 -0.9
100,561 - MPMX19_06603 0.30 -0.3
100,561 - MPMX19_06603 0.30 -0.8
100,561 - MPMX19_06603 0.30 -0.1
100,561 - MPMX19_06603 0.30 +0.2
100,561 - MPMX19_06603 0.30 +1.3
100,561 - MPMX19_06603 0.30 -0.5
100,561 - MPMX19_06603 0.30 -0.3
100,561 - MPMX19_06603 0.30 +0.4
100,561 - MPMX19_06603 0.30 +0.1
100,561 - MPMX19_06603 0.30 +1.0
100,561 - MPMX19_06603 0.30 -1.4
100,561 - MPMX19_06603 0.30 -0.8
100,561 - MPMX19_06603 0.30 +1.8
100,561 - MPMX19_06603 0.30 -0.2
100,561 - MPMX19_06603 0.30 -0.4
100,632 + MPMX19_06603 0.33 +0.1
100,632 + MPMX19_06603 0.33 -1.3
100,632 + MPMX19_06603 0.33 -2.0
100,632 + MPMX19_06603 0.33 -0.5
100,632 + MPMX19_06603 0.33 +0.8
100,632 + MPMX19_06603 0.33 +0.2
100,632 + MPMX19_06603 0.33 -0.5
100,632 + MPMX19_06603 0.33 +0.2
100,633 - MPMX19_06603 0.33 +0.1
100,633 - MPMX19_06603 0.33 -1.5
100,633 - MPMX19_06603 0.33 +1.2
100,633 - MPMX19_06603 0.33 -0.3
100,633 - MPMX19_06603 0.33 -0.9
100,633 - MPMX19_06603 0.33 -0.8
100,633 - MPMX19_06603 0.33 -1.2
100,633 - MPMX19_06603 0.33 -0.2
100,633 - MPMX19_06603 0.33 +0.2
100,633 - MPMX19_06603 0.33 +0.2
100,633 - MPMX19_06603 0.33 +1.6
100,782 + MPMX19_06603 0.40 -1.2
100,782 + MPMX19_06603 0.40 +0.0
100,782 + MPMX19_06603 0.40 -0.6
100,782 + MPMX19_06603 0.40 +0.4
100,782 + MPMX19_06603 0.40 -0.1
100,782 + MPMX19_06603 0.40 +0.6
100,782 + MPMX19_06603 0.40 +0.2
100,783 - MPMX19_06603 0.40 -1.1

Or see this region's nucleotide sequence