Experiment: NF_with10percent_fullN
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX19_04781 and MPMX19_04782 are separated by 224 nucleotides MPMX19_04782 and MPMX19_04783 are separated by 188 nucleotides
MPMX19_04781: MPMX19_04781 - hypothetical protein, at 176,700 to 177,782
_04781
MPMX19_04782: MPMX19_04782 - hypothetical protein, at 178,007 to 179,419
_04782
MPMX19_04783: MPMX19_04783 - Ubiquinone biosynthesis O-methyltransferase, mitochondrial, at 179,608 to 180,495
_04783
Position (kb) 178
179
180 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 177.013 kb on + strand, within MPMX19_04781 at 177.013 kb on + strand, within MPMX19_04781 at 177.013 kb on + strand, within MPMX19_04781 at 177.013 kb on + strand, within MPMX19_04781 at 177.014 kb on - strand, within MPMX19_04781 at 177.014 kb on - strand, within MPMX19_04781 at 177.014 kb on - strand, within MPMX19_04781 at 177.519 kb on + strand, within MPMX19_04781 at 177.572 kb on + strand, within MPMX19_04781 at 177.740 kb on + strand at 177.740 kb on + strand at 177.849 kb on + strand at 177.850 kb on - strand at 177.857 kb on + strand at 177.901 kb on - strand at 177.941 kb on + strand at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.226 kb on + strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.227 kb on - strand, within MPMX19_04782 at 178.347 kb on + strand, within MPMX19_04782 at 178.347 kb on + strand, within MPMX19_04782 at 178.348 kb on - strand, within MPMX19_04782 at 178.348 kb on - strand, within MPMX19_04782 at 178.348 kb on - strand, within MPMX19_04782 at 178.348 kb on - strand, within MPMX19_04782 at 178.431 kb on + strand, within MPMX19_04782 at 178.476 kb on + strand, within MPMX19_04782 at 178.476 kb on + strand, within MPMX19_04782 at 178.476 kb on + strand, within MPMX19_04782 at 178.476 kb on + strand, within MPMX19_04782 at 178.476 kb on + strand, within MPMX19_04782 at 178.476 kb on + strand, within MPMX19_04782 at 178.476 kb on + strand, within MPMX19_04782 at 178.476 kb on + strand, within MPMX19_04782 at 178.477 kb on - strand, within MPMX19_04782 at 178.477 kb on - strand, within MPMX19_04782 at 178.477 kb on - strand, within MPMX19_04782 at 178.477 kb on - strand, within MPMX19_04782 at 178.477 kb on - strand, within MPMX19_04782 at 178.477 kb on - strand, within MPMX19_04782 at 178.624 kb on - strand, within MPMX19_04782 at 178.719 kb on + strand, within MPMX19_04782 at 178.719 kb on + strand, within MPMX19_04782 at 178.719 kb on + strand, within MPMX19_04782 at 178.719 kb on + strand, within MPMX19_04782 at 178.719 kb on + strand, within MPMX19_04782 at 178.719 kb on + strand, within MPMX19_04782 at 178.719 kb on + strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.720 kb on - strand, within MPMX19_04782 at 178.827 kb on + strand, within MPMX19_04782 at 178.827 kb on + strand, within MPMX19_04782 at 178.827 kb on + strand, within MPMX19_04782 at 178.827 kb on + strand, within MPMX19_04782 at 178.827 kb on + strand, within MPMX19_04782 at 178.827 kb on + strand, within MPMX19_04782 at 178.827 kb on + strand, within MPMX19_04782 at 178.827 kb on + strand, within MPMX19_04782 at 178.827 kb on + strand, within MPMX19_04782 at 178.828 kb on - strand, within MPMX19_04782 at 178.828 kb on - strand, within MPMX19_04782 at 178.828 kb on - strand, within MPMX19_04782 at 178.828 kb on - strand, within MPMX19_04782 at 178.828 kb on - strand, within MPMX19_04782 at 178.836 kb on + strand, within MPMX19_04782 at 178.836 kb on + strand, within MPMX19_04782 at 178.836 kb on + strand, within MPMX19_04782 at 178.836 kb on + strand, within MPMX19_04782 at 178.836 kb on + strand, within MPMX19_04782 at 178.836 kb on + strand, within MPMX19_04782 at 178.836 kb on + strand, within MPMX19_04782 at 178.837 kb on - strand, within MPMX19_04782 at 178.837 kb on - strand, within MPMX19_04782 at 178.837 kb on - strand, within MPMX19_04782 at 178.837 kb on - strand, within MPMX19_04782 at 178.879 kb on - strand, within MPMX19_04782 at 178.938 kb on + strand, within MPMX19_04782 at 178.938 kb on + strand, within MPMX19_04782 at 178.939 kb on - strand, within MPMX19_04782 at 178.939 kb on - strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.947 kb on + strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 178.948 kb on - strand, within MPMX19_04782 at 179.016 kb on + strand, within MPMX19_04782 at 179.017 kb on - strand, within MPMX19_04782 at 179.040 kb on + strand, within MPMX19_04782 at 179.040 kb on + strand, within MPMX19_04782 at 179.041 kb on - strand, within MPMX19_04782 at 179.479 kb on + strand at 179.479 kb on + strand at 179.479 kb on + strand at 179.479 kb on + strand at 179.479 kb on + strand at 179.479 kb on + strand at 179.480 kb on - strand at 179.480 kb on - strand at 179.480 kb on - strand at 179.480 kb on - strand at 179.480 kb on - strand at 179.480 kb on - strand at 179.480 kb on - strand at 179.480 kb on - strand at 179.480 kb on - strand at 179.596 kb on + strand at 179.596 kb on + strand at 179.597 kb on - strand at 179.597 kb on - strand at 179.898 kb on + strand, within MPMX19_04783 at 179.899 kb on + strand, within MPMX19_04783 at 179.899 kb on + strand, within MPMX19_04783 at 179.900 kb on - strand, within MPMX19_04783 at 180.088 kb on + strand, within MPMX19_04783 at 180.088 kb on + strand, within MPMX19_04783 at 180.088 kb on + strand, within MPMX19_04783 at 180.088 kb on + strand, within MPMX19_04783 at 180.088 kb on + strand, within MPMX19_04783 at 180.088 kb on + strand, within MPMX19_04783 at 180.088 kb on + strand, within MPMX19_04783 at 180.088 kb on + strand, within MPMX19_04783 at 180.089 kb on - strand, within MPMX19_04783 at 180.089 kb on - strand, within MPMX19_04783 at 180.089 kb on - strand, within MPMX19_04783 at 180.089 kb on - strand, within MPMX19_04783 at 180.089 kb on - strand, within MPMX19_04783 at 180.089 kb on - strand, within MPMX19_04783 at 180.089 kb on - strand, within MPMX19_04783 at 180.136 kb on + strand, within MPMX19_04783 at 180.136 kb on + strand, within MPMX19_04783 at 180.136 kb on + strand, within MPMX19_04783 at 180.136 kb on + strand, within MPMX19_04783 at 180.136 kb on + strand, within MPMX19_04783 at 180.136 kb on + strand, within MPMX19_04783 at 180.136 kb on + strand, within MPMX19_04783 at 180.136 kb on + strand, within MPMX19_04783 at 180.137 kb on - strand, within MPMX19_04783 at 180.137 kb on - strand, within MPMX19_04783 at 180.137 kb on - strand, within MPMX19_04783 at 180.137 kb on - strand, within MPMX19_04783 at 180.137 kb on - strand, within MPMX19_04783 at 180.137 kb on - strand, within MPMX19_04783 at 180.137 kb on - strand, within MPMX19_04783 at 180.137 kb on - strand, within MPMX19_04783 at 180.241 kb on + strand, within MPMX19_04783 at 180.242 kb on - strand, within MPMX19_04783 at 180.242 kb on - strand, within MPMX19_04783 at 180.247 kb on + strand, within MPMX19_04783 at 180.248 kb on - strand, within MPMX19_04783 at 180.322 kb on + strand, within MPMX19_04783 at 180.322 kb on + strand, within MPMX19_04783 at 180.322 kb on + strand, within MPMX19_04783 at 180.323 kb on - strand, within MPMX19_04783 at 180.323 kb on - strand, within MPMX19_04783 at 180.323 kb on - strand, within MPMX19_04783 at 180.340 kb on + strand, within MPMX19_04783 at 180.340 kb on + strand, within MPMX19_04783
Per-strain Table
Position Strand Gene LocusTag Fraction NF_with10percent_fullN remove 177,013 + MPMX19_04781 0.29 -1.5 177,013 + MPMX19_04781 0.29 -1.9 177,013 + MPMX19_04781 0.29 +0.5 177,013 + MPMX19_04781 0.29 -0.6 177,014 - MPMX19_04781 0.29 -0.9 177,014 - MPMX19_04781 0.29 +1.0 177,014 - MPMX19_04781 0.29 +0.4 177,519 + MPMX19_04781 0.76 +0.2 177,572 + MPMX19_04781 0.81 +0.5 177,740 + +0.5 177,740 + -1.8 177,849 + -1.3 177,850 - -1.6 177,857 + +0.5 177,901 - -1.0 177,941 + -2.3 178,226 + MPMX19_04782 0.15 +0.4 178,226 + MPMX19_04782 0.15 +0.2 178,226 + MPMX19_04782 0.15 -1.5 178,226 + MPMX19_04782 0.15 -0.8 178,226 + MPMX19_04782 0.15 +0.4 178,226 + MPMX19_04782 0.15 -2.1 178,226 + MPMX19_04782 0.15 -0.4 178,226 + MPMX19_04782 0.15 -1.1 178,226 + MPMX19_04782 0.15 -1.7 178,226 + MPMX19_04782 0.15 -0.8 178,226 + MPMX19_04782 0.15 -0.3 178,226 + MPMX19_04782 0.15 -0.1 178,226 + MPMX19_04782 0.15 +0.5 178,226 + MPMX19_04782 0.15 -0.4 178,226 + MPMX19_04782 0.15 -0.3 178,226 + MPMX19_04782 0.15 +0.8 178,226 + MPMX19_04782 0.15 -0.5 178,226 + MPMX19_04782 0.15 -0.8 178,226 + MPMX19_04782 0.15 -1.3 178,226 + MPMX19_04782 0.15 -0.5 178,227 - MPMX19_04782 0.16 +0.3 178,227 - MPMX19_04782 0.16 +1.1 178,227 - MPMX19_04782 0.16 +0.5 178,227 - MPMX19_04782 0.16 -0.5 178,227 - MPMX19_04782 0.16 -1.2 178,227 - MPMX19_04782 0.16 -0.3 178,227 - MPMX19_04782 0.16 +1.1 178,227 - MPMX19_04782 0.16 +0.5 178,227 - MPMX19_04782 0.16 -0.1 178,227 - MPMX19_04782 0.16 -0.2 178,227 - MPMX19_04782 0.16 -1.5 178,227 - MPMX19_04782 0.16 -1.6 178,227 - MPMX19_04782 0.16 -0.3 178,227 - MPMX19_04782 0.16 -0.1 178,227 - MPMX19_04782 0.16 -1.5 178,227 - MPMX19_04782 0.16 +0.9 178,227 - MPMX19_04782 0.16 +1.5 178,227 - MPMX19_04782 0.16 -0.2 178,347 + MPMX19_04782 0.24 -0.5 178,347 + MPMX19_04782 0.24 -0.5 178,348 - MPMX19_04782 0.24 -0.9 178,348 - MPMX19_04782 0.24 -0.8 178,348 - MPMX19_04782 0.24 -0.9 178,348 - MPMX19_04782 0.24 -0.8 178,431 + MPMX19_04782 0.30 +1.3 178,476 + MPMX19_04782 0.33 -0.8 178,476 + MPMX19_04782 0.33 -0.1 178,476 + MPMX19_04782 0.33 -0.6 178,476 + MPMX19_04782 0.33 -1.8 178,476 + MPMX19_04782 0.33 +0.7 178,476 + MPMX19_04782 0.33 -0.4 178,476 + MPMX19_04782 0.33 -1.1 178,476 + MPMX19_04782 0.33 -0.3 178,477 - MPMX19_04782 0.33 -0.6 178,477 - MPMX19_04782 0.33 -0.6 178,477 - MPMX19_04782 0.33 -0.5 178,477 - MPMX19_04782 0.33 -0.8 178,477 - MPMX19_04782 0.33 -1.5 178,477 - MPMX19_04782 0.33 -1.8 178,624 - MPMX19_04782 0.44 -1.2 178,719 + MPMX19_04782 0.50 -1.8 178,719 + MPMX19_04782 0.50 +0.2 178,719 + MPMX19_04782 0.50 +0.9 178,719 + MPMX19_04782 0.50 +0.3 178,719 + MPMX19_04782 0.50 -1.5 178,719 + MPMX19_04782 0.50 -0.7 178,719 + MPMX19_04782 0.50 +1.5 178,720 - MPMX19_04782 0.50 +0.3 178,720 - MPMX19_04782 0.50 -1.5 178,720 - MPMX19_04782 0.50 -0.8 178,720 - MPMX19_04782 0.50 -1.5 178,720 - MPMX19_04782 0.50 -0.1 178,720 - MPMX19_04782 0.50 +0.5 178,720 - MPMX19_04782 0.50 -1.1 178,720 - MPMX19_04782 0.50 -1.1 178,720 - MPMX19_04782 0.50 -1.3 178,720 - MPMX19_04782 0.50 -1.6 178,720 - MPMX19_04782 0.50 +0.2 178,720 - MPMX19_04782 0.50 -0.5 178,827 + MPMX19_04782 0.58 +0.3 178,827 + MPMX19_04782 0.58 -1.0 178,827 + MPMX19_04782 0.58 -0.6 178,827 + MPMX19_04782 0.58 -0.0 178,827 + MPMX19_04782 0.58 +0.5 178,827 + MPMX19_04782 0.58 -1.8 178,827 + MPMX19_04782 0.58 -1.3 178,827 + MPMX19_04782 0.58 -0.8 178,827 + MPMX19_04782 0.58 -1.3 178,828 - MPMX19_04782 0.58 -1.1 178,828 - MPMX19_04782 0.58 -1.6 178,828 - MPMX19_04782 0.58 -1.0 178,828 - MPMX19_04782 0.58 -0.1 178,828 - MPMX19_04782 0.58 -0.8 178,836 + MPMX19_04782 0.59 -0.5 178,836 + MPMX19_04782 0.59 -0.8 178,836 + MPMX19_04782 0.59 +0.5 178,836 + MPMX19_04782 0.59 +0.2 178,836 + MPMX19_04782 0.59 +0.5 178,836 + MPMX19_04782 0.59 -0.1 178,836 + MPMX19_04782 0.59 -0.5 178,837 - MPMX19_04782 0.59 -0.6 178,837 - MPMX19_04782 0.59 +1.9 178,837 - MPMX19_04782 0.59 -0.5 178,837 - MPMX19_04782 0.59 -1.9 178,879 - MPMX19_04782 0.62 -1.5 178,938 + MPMX19_04782 0.66 -1.9 178,938 + MPMX19_04782 0.66 -1.1 178,939 - MPMX19_04782 0.66 +0.5 178,939 - MPMX19_04782 0.66 -0.8 178,947 + MPMX19_04782 0.67 -1.3 178,947 + MPMX19_04782 0.67 -1.8 178,947 + MPMX19_04782 0.67 -1.5 178,947 + MPMX19_04782 0.67 -1.4 178,947 + MPMX19_04782 0.67 +0.2 178,947 + MPMX19_04782 0.67 +0.1 178,947 + MPMX19_04782 0.67 -0.6 178,947 + MPMX19_04782 0.67 +0.3 178,947 + MPMX19_04782 0.67 +0.8 178,947 + MPMX19_04782 0.67 +0.2 178,947 + MPMX19_04782 0.67 -1.1 178,947 + MPMX19_04782 0.67 +0.5 178,947 + MPMX19_04782 0.67 -2.3 178,948 - MPMX19_04782 0.67 +0.1 178,948 - MPMX19_04782 0.67 -1.3 178,948 - MPMX19_04782 0.67 -0.3 178,948 - MPMX19_04782 0.67 +0.9 178,948 - MPMX19_04782 0.67 +1.5 178,948 - MPMX19_04782 0.67 -0.5 178,948 - MPMX19_04782 0.67 -0.8 178,948 - MPMX19_04782 0.67 -2.6 178,948 - MPMX19_04782 0.67 -0.8 178,948 - MPMX19_04782 0.67 -0.4 178,948 - MPMX19_04782 0.67 -1.6 178,948 - MPMX19_04782 0.67 -2.4 178,948 - MPMX19_04782 0.67 -0.8 178,948 - MPMX19_04782 0.67 -0.6 178,948 - MPMX19_04782 0.67 +0.1 178,948 - MPMX19_04782 0.67 -0.3 179,016 + MPMX19_04782 0.71 -0.5 179,017 - MPMX19_04782 0.71 +0.1 179,040 + MPMX19_04782 0.73 +1.5 179,040 + MPMX19_04782 0.73 -0.5 179,041 - MPMX19_04782 0.73 -0.1 179,479 + +0.2 179,479 + -3.3 179,479 + +1.5 179,479 + -0.3 179,479 + -2.5 179,479 + +0.5 179,480 - +0.5 179,480 - -0.8 179,480 - +0.7 179,480 - +1.1 179,480 - -0.1 179,480 - -1.9 179,480 - -2.0 179,480 - -0.5 179,480 - -2.2 179,596 + -1.2 179,596 + +0.4 179,597 - -0.8 179,597 - +0.5 179,898 + MPMX19_04783 0.33 -0.5 179,899 + MPMX19_04783 0.33 +0.2 179,899 + MPMX19_04783 0.33 -0.3 179,900 - MPMX19_04783 0.33 -0.5 180,088 + MPMX19_04783 0.54 -0.8 180,088 + MPMX19_04783 0.54 -1.1 180,088 + MPMX19_04783 0.54 -1.1 180,088 + MPMX19_04783 0.54 -1.7 180,088 + MPMX19_04783 0.54 +0.9 180,088 + MPMX19_04783 0.54 -0.3 180,088 + MPMX19_04783 0.54 -1.1 180,088 + MPMX19_04783 0.54 -0.3 180,089 - MPMX19_04783 0.54 -0.1 180,089 - MPMX19_04783 0.54 +0.5 180,089 - MPMX19_04783 0.54 -1.1 180,089 - MPMX19_04783 0.54 -0.5 180,089 - MPMX19_04783 0.54 -0.3 180,089 - MPMX19_04783 0.54 +0.5 180,089 - MPMX19_04783 0.54 +0.7 180,136 + MPMX19_04783 0.59 -2.3 180,136 + MPMX19_04783 0.59 -1.5 180,136 + MPMX19_04783 0.59 -2.6 180,136 + MPMX19_04783 0.59 +2.1 180,136 + MPMX19_04783 0.59 -0.3 180,136 + MPMX19_04783 0.59 -0.9 180,136 + MPMX19_04783 0.59 -0.1 180,136 + MPMX19_04783 0.59 -0.8 180,137 - MPMX19_04783 0.60 +0.5 180,137 - MPMX19_04783 0.60 -0.5 180,137 - MPMX19_04783 0.60 -1.5 180,137 - MPMX19_04783 0.60 -0.9 180,137 - MPMX19_04783 0.60 -1.1 180,137 - MPMX19_04783 0.60 +0.5 180,137 - MPMX19_04783 0.60 -0.5 180,137 - MPMX19_04783 0.60 +0.4 180,241 + MPMX19_04783 0.71 -0.1 180,242 - MPMX19_04783 0.71 -0.6 180,242 - MPMX19_04783 0.71 -0.1 180,247 + MPMX19_04783 0.72 -0.1 180,248 - MPMX19_04783 0.72 +1.5 180,322 + MPMX19_04783 0.80 -1.1 180,322 + MPMX19_04783 0.80 +0.7 180,322 + MPMX19_04783 0.80 -1.0 180,323 - MPMX19_04783 0.81 -0.5 180,323 - MPMX19_04783 0.81 -2.8 180,323 - MPMX19_04783 0.81 -0.8 180,340 + MPMX19_04783 0.82 -0.1 180,340 + MPMX19_04783 0.82 -0.3
Or see this region's nucleotide sequence