Experiment: Thymine 0.793 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS09505 and A4249_RS09510 are separated by 52 nucleotides A4249_RS09510 and A4249_RS09515 are separated by 7 nucleotides A4249_RS09515 and A4249_RS09520 are separated by 62 nucleotides
A4249_RS09505: A4249_RS09505 - DNA-3-methyladenine glycosylase I, at 1,898,825 to 1,899,433
_RS09505
A4249_RS09510: A4249_RS09510 - ribonuclease HII, at 1,899,486 to 1,900,067
_RS09510
A4249_RS09515: A4249_RS09515 - hypothetical protein, at 1,900,075 to 1,900,353
_RS09515
A4249_RS09520: A4249_RS09520 - DNA adenine methylase, at 1,900,416 to 1,901,453
_RS09520
Position (kb)
1900
1901 Strain fitness (log2 ratio)
-2
-1
0
1 at 1899.239 kb on + strand, within A4249_RS09505 at 1899.240 kb on - strand, within A4249_RS09505 at 1899.248 kb on + strand, within A4249_RS09505 at 1899.249 kb on - strand, within A4249_RS09505 at 1899.341 kb on + strand, within A4249_RS09505 at 1899.341 kb on + strand, within A4249_RS09505 at 1899.341 kb on + strand, within A4249_RS09505 at 1899.342 kb on - strand, within A4249_RS09505 at 1899.342 kb on - strand, within A4249_RS09505 at 1899.432 kb on - strand at 1899.571 kb on + strand, within A4249_RS09510 at 1899.571 kb on + strand, within A4249_RS09510 at 1899.572 kb on - strand, within A4249_RS09510 at 1899.572 kb on - strand, within A4249_RS09510 at 1899.582 kb on + strand, within A4249_RS09510 at 1899.804 kb on + strand, within A4249_RS09510 at 1899.804 kb on + strand, within A4249_RS09510 at 1899.805 kb on - strand, within A4249_RS09510 at 1899.805 kb on - strand, within A4249_RS09510 at 1899.805 kb on - strand, within A4249_RS09510 at 1899.805 kb on - strand, within A4249_RS09510 at 1899.862 kb on + strand, within A4249_RS09510 at 1899.942 kb on + strand, within A4249_RS09510 at 1899.960 kb on - strand, within A4249_RS09510 at 1899.966 kb on + strand, within A4249_RS09510 at 1899.966 kb on + strand, within A4249_RS09510 at 1899.966 kb on + strand, within A4249_RS09510 at 1899.966 kb on + strand, within A4249_RS09510 at 1899.967 kb on - strand, within A4249_RS09510 at 1899.967 kb on - strand, within A4249_RS09510 at 1899.967 kb on - strand, within A4249_RS09510 at 1900.224 kb on - strand, within A4249_RS09515 at 1900.305 kb on - strand, within A4249_RS09515 at 1900.305 kb on - strand, within A4249_RS09515 at 1900.305 kb on - strand, within A4249_RS09515 at 1900.337 kb on + strand at 1900.337 kb on + strand at 1900.337 kb on + strand at 1900.337 kb on + strand at 1900.338 kb on - strand at 1900.338 kb on - strand at 1900.353 kb on + strand at 1900.354 kb on - strand at 1900.391 kb on + strand at 1900.417 kb on + strand at 1900.417 kb on + strand at 1900.417 kb on + strand at 1900.417 kb on + strand at 1900.418 kb on - strand at 1900.418 kb on - strand at 1900.514 kb on + strand at 1900.517 kb on + strand at 1900.518 kb on - strand at 1900.518 kb on - strand at 1900.543 kb on + strand, within A4249_RS09520 at 1900.544 kb on - strand, within A4249_RS09520 at 1900.544 kb on - strand, within A4249_RS09520 at 1900.544 kb on - strand, within A4249_RS09520 at 1900.544 kb on - strand, within A4249_RS09520 at 1900.544 kb on - strand, within A4249_RS09520 at 1900.621 kb on + strand, within A4249_RS09520 at 1900.621 kb on + strand, within A4249_RS09520 at 1900.762 kb on + strand, within A4249_RS09520 at 1900.762 kb on + strand, within A4249_RS09520 at 1900.762 kb on + strand, within A4249_RS09520 at 1900.762 kb on + strand, within A4249_RS09520 at 1900.763 kb on - strand, within A4249_RS09520 at 1900.807 kb on + strand, within A4249_RS09520 at 1900.807 kb on + strand, within A4249_RS09520 at 1900.807 kb on + strand, within A4249_RS09520 at 1900.807 kb on + strand, within A4249_RS09520 at 1900.808 kb on - strand, within A4249_RS09520 at 1900.808 kb on - strand, within A4249_RS09520 at 1900.808 kb on - strand, within A4249_RS09520 at 1900.808 kb on - strand, within A4249_RS09520 at 1900.820 kb on - strand, within A4249_RS09520 at 1900.820 kb on - strand, within A4249_RS09520 at 1900.834 kb on + strand, within A4249_RS09520 at 1900.834 kb on + strand, within A4249_RS09520 at 1900.834 kb on + strand, within A4249_RS09520 at 1900.835 kb on - strand, within A4249_RS09520 at 1900.835 kb on - strand, within A4249_RS09520 at 1900.850 kb on - strand, within A4249_RS09520 at 1900.988 kb on + strand, within A4249_RS09520 at 1900.988 kb on + strand, within A4249_RS09520 at 1900.989 kb on - strand, within A4249_RS09520 at 1900.989 kb on - strand, within A4249_RS09520 at 1900.989 kb on - strand, within A4249_RS09520 at 1900.989 kb on - strand, within A4249_RS09520 at 1900.990 kb on + strand, within A4249_RS09520 at 1900.990 kb on + strand, within A4249_RS09520 at 1900.991 kb on - strand, within A4249_RS09520 at 1900.991 kb on - strand, within A4249_RS09520 at 1900.991 kb on - strand, within A4249_RS09520 at 1900.991 kb on - strand, within A4249_RS09520 at 1900.991 kb on - strand, within A4249_RS09520 at 1900.991 kb on - strand, within A4249_RS09520 at 1900.991 kb on - strand, within A4249_RS09520 at 1901.257 kb on + strand, within A4249_RS09520 at 1901.257 kb on + strand, within A4249_RS09520 at 1901.257 kb on + strand, within A4249_RS09520 at 1901.257 kb on + strand, within A4249_RS09520 at 1901.257 kb on + strand, within A4249_RS09520 at 1901.257 kb on + strand, within A4249_RS09520 at 1901.257 kb on + strand, within A4249_RS09520 at 1901.258 kb on - strand, within A4249_RS09520 at 1901.258 kb on - strand, within A4249_RS09520 at 1901.258 kb on - strand, within A4249_RS09520 at 1901.258 kb on - strand, within A4249_RS09520 at 1901.258 kb on - strand, within A4249_RS09520 at 1901.258 kb on - strand, within A4249_RS09520 at 1901.258 kb on - strand, within A4249_RS09520 at 1901.258 kb on - strand, within A4249_RS09520
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine 0.793 mM (C) remove 1,899,239 + A4249_RS09505 0.68 +0.5 1,899,240 - A4249_RS09505 0.68 -0.6 1,899,248 + A4249_RS09505 0.69 -1.6 1,899,249 - A4249_RS09505 0.70 +0.9 1,899,341 + A4249_RS09505 0.85 +0.5 1,899,341 + A4249_RS09505 0.85 +0.2 1,899,341 + A4249_RS09505 0.85 +0.2 1,899,342 - A4249_RS09505 0.85 -0.1 1,899,342 - A4249_RS09505 0.85 -0.8 1,899,432 - -0.5 1,899,571 + A4249_RS09510 0.15 -0.4 1,899,571 + A4249_RS09510 0.15 -0.3 1,899,572 - A4249_RS09510 0.15 +0.3 1,899,572 - A4249_RS09510 0.15 -0.2 1,899,582 + A4249_RS09510 0.16 +0.4 1,899,804 + A4249_RS09510 0.55 +0.8 1,899,804 + A4249_RS09510 0.55 +0.7 1,899,805 - A4249_RS09510 0.55 -0.2 1,899,805 - A4249_RS09510 0.55 -0.0 1,899,805 - A4249_RS09510 0.55 +0.1 1,899,805 - A4249_RS09510 0.55 -0.5 1,899,862 + A4249_RS09510 0.65 +0.6 1,899,942 + A4249_RS09510 0.78 +0.2 1,899,960 - A4249_RS09510 0.81 +0.4 1,899,966 + A4249_RS09510 0.82 -0.0 1,899,966 + A4249_RS09510 0.82 -0.2 1,899,966 + A4249_RS09510 0.82 -0.3 1,899,966 + A4249_RS09510 0.82 +0.2 1,899,967 - A4249_RS09510 0.83 +0.2 1,899,967 - A4249_RS09510 0.83 -0.5 1,899,967 - A4249_RS09510 0.83 -1.5 1,900,224 - A4249_RS09515 0.53 +0.5 1,900,305 - A4249_RS09515 0.82 -0.8 1,900,305 - A4249_RS09515 0.82 -0.4 1,900,305 - A4249_RS09515 0.82 -0.0 1,900,337 + +0.1 1,900,337 + -0.4 1,900,337 + +0.6 1,900,337 + +1.3 1,900,338 - +0.2 1,900,338 - -0.4 1,900,353 + +0.7 1,900,354 - +0.3 1,900,391 + -1.5 1,900,417 + -0.2 1,900,417 + +0.3 1,900,417 + +0.2 1,900,417 + -0.5 1,900,418 - +0.3 1,900,418 - -0.1 1,900,514 + -1.3 1,900,517 + -0.6 1,900,518 - +0.5 1,900,518 - -2.0 1,900,543 + A4249_RS09520 0.12 +1.1 1,900,544 - A4249_RS09520 0.12 +1.0 1,900,544 - A4249_RS09520 0.12 +0.6 1,900,544 - A4249_RS09520 0.12 +0.0 1,900,544 - A4249_RS09520 0.12 -1.7 1,900,544 - A4249_RS09520 0.12 -1.5 1,900,621 + A4249_RS09520 0.20 -0.4 1,900,621 + A4249_RS09520 0.20 +0.3 1,900,762 + A4249_RS09520 0.33 -0.1 1,900,762 + A4249_RS09520 0.33 +1.0 1,900,762 + A4249_RS09520 0.33 +0.3 1,900,762 + A4249_RS09520 0.33 +0.0 1,900,763 - A4249_RS09520 0.33 -0.3 1,900,807 + A4249_RS09520 0.38 -0.4 1,900,807 + A4249_RS09520 0.38 +0.5 1,900,807 + A4249_RS09520 0.38 -0.2 1,900,807 + A4249_RS09520 0.38 -1.5 1,900,808 - A4249_RS09520 0.38 -0.9 1,900,808 - A4249_RS09520 0.38 +0.1 1,900,808 - A4249_RS09520 0.38 +0.9 1,900,808 - A4249_RS09520 0.38 +0.8 1,900,820 - A4249_RS09520 0.39 +0.9 1,900,820 - A4249_RS09520 0.39 +0.2 1,900,834 + A4249_RS09520 0.40 -0.6 1,900,834 + A4249_RS09520 0.40 +0.5 1,900,834 + A4249_RS09520 0.40 -0.9 1,900,835 - A4249_RS09520 0.40 +1.0 1,900,835 - A4249_RS09520 0.40 -2.4 1,900,850 - A4249_RS09520 0.42 +0.5 1,900,988 + A4249_RS09520 0.55 +0.2 1,900,988 + A4249_RS09520 0.55 -0.8 1,900,989 - A4249_RS09520 0.55 -0.2 1,900,989 - A4249_RS09520 0.55 +0.5 1,900,989 - A4249_RS09520 0.55 +0.1 1,900,989 - A4249_RS09520 0.55 +0.3 1,900,990 + A4249_RS09520 0.55 -0.5 1,900,990 + A4249_RS09520 0.55 -0.6 1,900,991 - A4249_RS09520 0.55 -0.5 1,900,991 - A4249_RS09520 0.55 +0.3 1,900,991 - A4249_RS09520 0.55 +0.4 1,900,991 - A4249_RS09520 0.55 -0.8 1,900,991 - A4249_RS09520 0.55 -0.9 1,900,991 - A4249_RS09520 0.55 -0.5 1,900,991 - A4249_RS09520 0.55 -0.8 1,901,257 + A4249_RS09520 0.81 -0.5 1,901,257 + A4249_RS09520 0.81 +0.5 1,901,257 + A4249_RS09520 0.81 +0.3 1,901,257 + A4249_RS09520 0.81 -0.4 1,901,257 + A4249_RS09520 0.81 -0.1 1,901,257 + A4249_RS09520 0.81 -0.2 1,901,257 + A4249_RS09520 0.81 -0.6 1,901,258 - A4249_RS09520 0.81 +0.0 1,901,258 - A4249_RS09520 0.81 -0.3 1,901,258 - A4249_RS09520 0.81 -0.1 1,901,258 - A4249_RS09520 0.81 +0.9 1,901,258 - A4249_RS09520 0.81 -0.1 1,901,258 - A4249_RS09520 0.81 +0.8 1,901,258 - A4249_RS09520 0.81 -0.7 1,901,258 - A4249_RS09520 0.81 -2.2
Or see this region's nucleotide sequence