Experiment: Thymine 0.793 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS03475 and A4249_RS03480 are separated by 29 nucleotides A4249_RS03480 and A4249_RS03485 are separated by 408 nucleotides
A4249_RS03475: A4249_RS03475 - glutathione-dependent formaldehyde dehydrogenase, at 724,201 to 725,376
_RS03475
A4249_RS03480: A4249_RS03480 - SRPBCC family protein, at 725,406 to 725,975
_RS03480
A4249_RS03485: A4249_RS03485 - SDR family NAD(P)-dependent oxidoreductase, at 726,384 to 726,899
_RS03485
Position (kb)
725
726 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 724.442 kb on + strand, within A4249_RS03475 at 724.442 kb on + strand, within A4249_RS03475 at 724.443 kb on - strand, within A4249_RS03475 at 724.443 kb on - strand, within A4249_RS03475 at 724.443 kb on - strand, within A4249_RS03475 at 724.443 kb on - strand, within A4249_RS03475 at 724.464 kb on + strand, within A4249_RS03475 at 724.464 kb on + strand, within A4249_RS03475 at 724.464 kb on + strand, within A4249_RS03475 at 724.465 kb on - strand, within A4249_RS03475 at 724.465 kb on - strand, within A4249_RS03475 at 724.465 kb on - strand, within A4249_RS03475 at 724.465 kb on - strand, within A4249_RS03475 at 724.465 kb on - strand, within A4249_RS03475 at 724.465 kb on - strand, within A4249_RS03475 at 724.554 kb on + strand, within A4249_RS03475 at 724.554 kb on + strand, within A4249_RS03475 at 724.554 kb on + strand, within A4249_RS03475 at 724.555 kb on - strand, within A4249_RS03475 at 724.664 kb on + strand, within A4249_RS03475 at 724.664 kb on + strand, within A4249_RS03475 at 724.664 kb on + strand, within A4249_RS03475 at 724.664 kb on + strand, within A4249_RS03475 at 724.665 kb on - strand, within A4249_RS03475 at 724.665 kb on - strand, within A4249_RS03475 at 724.665 kb on - strand, within A4249_RS03475 at 724.665 kb on - strand, within A4249_RS03475 at 724.665 kb on - strand, within A4249_RS03475 at 724.665 kb on - strand, within A4249_RS03475 at 724.665 kb on - strand, within A4249_RS03475 at 724.665 kb on - strand, within A4249_RS03475 at 724.665 kb on - strand, within A4249_RS03475 at 724.861 kb on + strand, within A4249_RS03475 at 724.861 kb on + strand, within A4249_RS03475 at 724.861 kb on + strand, within A4249_RS03475 at 724.862 kb on - strand, within A4249_RS03475 at 724.931 kb on + strand, within A4249_RS03475 at 724.946 kb on + strand, within A4249_RS03475 at 724.946 kb on + strand, within A4249_RS03475 at 724.988 kb on + strand, within A4249_RS03475 at 724.988 kb on + strand, within A4249_RS03475 at 725.018 kb on + strand, within A4249_RS03475 at 725.018 kb on + strand, within A4249_RS03475 at 725.019 kb on - strand, within A4249_RS03475 at 725.019 kb on - strand, within A4249_RS03475 at 725.019 kb on - strand, within A4249_RS03475 at 725.331 kb on + strand at 725.331 kb on + strand at 725.388 kb on + strand at 725.389 kb on - strand at 725.389 kb on - strand at 725.389 kb on - strand at 725.575 kb on + strand, within A4249_RS03480 at 725.575 kb on + strand, within A4249_RS03480 at 725.575 kb on + strand, within A4249_RS03480 at 725.575 kb on + strand, within A4249_RS03480 at 725.575 kb on + strand, within A4249_RS03480 at 725.576 kb on - strand, within A4249_RS03480 at 725.576 kb on - strand, within A4249_RS03480 at 725.666 kb on + strand, within A4249_RS03480 at 725.666 kb on + strand, within A4249_RS03480 at 725.666 kb on + strand, within A4249_RS03480 at 725.666 kb on + strand, within A4249_RS03480 at 725.667 kb on - strand, within A4249_RS03480 at 725.667 kb on - strand, within A4249_RS03480 at 725.667 kb on - strand, within A4249_RS03480 at 725.667 kb on - strand, within A4249_RS03480 at 725.667 kb on - strand, within A4249_RS03480 at 725.667 kb on - strand, within A4249_RS03480 at 725.724 kb on + strand, within A4249_RS03480 at 725.816 kb on + strand, within A4249_RS03480 at 725.816 kb on + strand, within A4249_RS03480 at 725.816 kb on + strand, within A4249_RS03480 at 725.816 kb on + strand, within A4249_RS03480 at 725.817 kb on - strand, within A4249_RS03480 at 725.842 kb on + strand, within A4249_RS03480 at 725.842 kb on + strand, within A4249_RS03480 at 725.842 kb on + strand, within A4249_RS03480 at 725.843 kb on - strand, within A4249_RS03480 at 725.843 kb on - strand, within A4249_RS03480 at 725.896 kb on + strand, within A4249_RS03480 at 725.896 kb on + strand, within A4249_RS03480 at 725.896 kb on + strand, within A4249_RS03480 at 725.897 kb on - strand, within A4249_RS03480 at 725.975 kb on + strand at 725.975 kb on + strand at 725.976 kb on - strand at 725.976 kb on - strand at 725.981 kb on + strand at 725.981 kb on + strand at 725.981 kb on + strand at 725.981 kb on + strand at 725.981 kb on + strand at 725.982 kb on - strand at 725.982 kb on - strand at 726.079 kb on + strand at 726.079 kb on + strand at 726.079 kb on + strand at 726.080 kb on - strand at 726.080 kb on - strand at 726.321 kb on + strand at 726.321 kb on + strand at 726.321 kb on + strand at 726.321 kb on + strand at 726.321 kb on + strand at 726.321 kb on + strand at 726.322 kb on - strand at 726.322 kb on - strand at 726.443 kb on + strand, within A4249_RS03485 at 726.443 kb on + strand, within A4249_RS03485 at 726.444 kb on - strand, within A4249_RS03485 at 726.444 kb on - strand, within A4249_RS03485 at 726.444 kb on - strand, within A4249_RS03485 at 726.444 kb on - strand, within A4249_RS03485 at 726.485 kb on + strand, within A4249_RS03485 at 726.485 kb on + strand, within A4249_RS03485 at 726.485 kb on + strand, within A4249_RS03485 at 726.486 kb on - strand, within A4249_RS03485 at 726.486 kb on - strand, within A4249_RS03485 at 726.486 kb on - strand, within A4249_RS03485 at 726.486 kb on - strand, within A4249_RS03485 at 726.564 kb on + strand, within A4249_RS03485 at 726.564 kb on + strand, within A4249_RS03485 at 726.564 kb on + strand, within A4249_RS03485 at 726.564 kb on + strand, within A4249_RS03485 at 726.564 kb on + strand, within A4249_RS03485 at 726.565 kb on - strand, within A4249_RS03485 at 726.565 kb on - strand, within A4249_RS03485 at 726.759 kb on + strand, within A4249_RS03485 at 726.759 kb on + strand, within A4249_RS03485 at 726.760 kb on - strand, within A4249_RS03485 at 726.897 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine 0.793 mM (C) remove 724,442 + A4249_RS03475 0.20 +0.1 724,442 + A4249_RS03475 0.20 -0.6 724,443 - A4249_RS03475 0.21 +1.1 724,443 - A4249_RS03475 0.21 -0.9 724,443 - A4249_RS03475 0.21 +0.9 724,443 - A4249_RS03475 0.21 +0.1 724,464 + A4249_RS03475 0.22 +0.2 724,464 + A4249_RS03475 0.22 -0.5 724,464 + A4249_RS03475 0.22 -0.2 724,465 - A4249_RS03475 0.22 +0.8 724,465 - A4249_RS03475 0.22 +0.2 724,465 - A4249_RS03475 0.22 +0.2 724,465 - A4249_RS03475 0.22 -2.0 724,465 - A4249_RS03475 0.22 -0.0 724,465 - A4249_RS03475 0.22 +0.3 724,554 + A4249_RS03475 0.30 +0.3 724,554 + A4249_RS03475 0.30 +1.9 724,554 + A4249_RS03475 0.30 +0.1 724,555 - A4249_RS03475 0.30 +0.5 724,664 + A4249_RS03475 0.39 -0.1 724,664 + A4249_RS03475 0.39 +0.8 724,664 + A4249_RS03475 0.39 -0.2 724,664 + A4249_RS03475 0.39 -0.7 724,665 - A4249_RS03475 0.39 -0.1 724,665 - A4249_RS03475 0.39 -0.3 724,665 - A4249_RS03475 0.39 -0.0 724,665 - A4249_RS03475 0.39 -0.2 724,665 - A4249_RS03475 0.39 +0.4 724,665 - A4249_RS03475 0.39 -0.1 724,665 - A4249_RS03475 0.39 -0.1 724,665 - A4249_RS03475 0.39 -1.0 724,665 - A4249_RS03475 0.39 +0.3 724,861 + A4249_RS03475 0.56 +0.3 724,861 + A4249_RS03475 0.56 -0.4 724,861 + A4249_RS03475 0.56 +0.1 724,862 - A4249_RS03475 0.56 -1.5 724,931 + A4249_RS03475 0.62 -0.5 724,946 + A4249_RS03475 0.63 -0.9 724,946 + A4249_RS03475 0.63 -0.1 724,988 + A4249_RS03475 0.67 -1.4 724,988 + A4249_RS03475 0.67 +0.6 725,018 + A4249_RS03475 0.69 -0.3 725,018 + A4249_RS03475 0.69 +0.8 725,019 - A4249_RS03475 0.70 -0.7 725,019 - A4249_RS03475 0.70 -0.4 725,019 - A4249_RS03475 0.70 -0.5 725,331 + -1.4 725,331 + -0.4 725,388 + +1.0 725,389 - +0.6 725,389 - +0.5 725,389 - +0.7 725,575 + A4249_RS03480 0.30 +0.6 725,575 + A4249_RS03480 0.30 +0.3 725,575 + A4249_RS03480 0.30 -0.7 725,575 + A4249_RS03480 0.30 +0.2 725,575 + A4249_RS03480 0.30 +0.1 725,576 - A4249_RS03480 0.30 +0.3 725,576 - A4249_RS03480 0.30 +0.2 725,666 + A4249_RS03480 0.46 -0.9 725,666 + A4249_RS03480 0.46 -0.6 725,666 + A4249_RS03480 0.46 -0.4 725,666 + A4249_RS03480 0.46 +0.2 725,667 - A4249_RS03480 0.46 +0.7 725,667 - A4249_RS03480 0.46 -0.1 725,667 - A4249_RS03480 0.46 -0.2 725,667 - A4249_RS03480 0.46 -1.2 725,667 - A4249_RS03480 0.46 -1.1 725,667 - A4249_RS03480 0.46 +0.3 725,724 + A4249_RS03480 0.56 +0.1 725,816 + A4249_RS03480 0.72 -0.2 725,816 + A4249_RS03480 0.72 -0.7 725,816 + A4249_RS03480 0.72 -0.2 725,816 + A4249_RS03480 0.72 +0.1 725,817 - A4249_RS03480 0.72 -1.6 725,842 + A4249_RS03480 0.76 -1.4 725,842 + A4249_RS03480 0.76 -0.1 725,842 + A4249_RS03480 0.76 -0.3 725,843 - A4249_RS03480 0.77 +0.5 725,843 - A4249_RS03480 0.77 -0.2 725,896 + A4249_RS03480 0.86 -0.5 725,896 + A4249_RS03480 0.86 -1.1 725,896 + A4249_RS03480 0.86 +0.5 725,897 - A4249_RS03480 0.86 -0.5 725,975 + -2.7 725,975 + -0.2 725,976 - -0.4 725,976 - -1.6 725,981 + +0.1 725,981 + +0.4 725,981 + -0.8 725,981 + -0.4 725,981 + +1.3 725,982 - -0.1 725,982 - +0.8 726,079 + +0.4 726,079 + +0.1 726,079 + -0.2 726,080 - +0.4 726,080 - +0.3 726,321 + +1.4 726,321 + +0.2 726,321 + +0.0 726,321 + +0.1 726,321 + -1.3 726,321 + +0.3 726,322 - -2.4 726,322 - +0.9 726,443 + A4249_RS03485 0.11 -1.1 726,443 + A4249_RS03485 0.11 -0.5 726,444 - A4249_RS03485 0.12 -0.1 726,444 - A4249_RS03485 0.12 +0.1 726,444 - A4249_RS03485 0.12 +0.3 726,444 - A4249_RS03485 0.12 -0.1 726,485 + A4249_RS03485 0.20 -0.1 726,485 + A4249_RS03485 0.20 +0.8 726,485 + A4249_RS03485 0.20 -0.1 726,486 - A4249_RS03485 0.20 +0.5 726,486 - A4249_RS03485 0.20 +1.3 726,486 - A4249_RS03485 0.20 -0.3 726,486 - A4249_RS03485 0.20 -0.0 726,564 + A4249_RS03485 0.35 -0.1 726,564 + A4249_RS03485 0.35 -0.3 726,564 + A4249_RS03485 0.35 +0.1 726,564 + A4249_RS03485 0.35 -0.7 726,564 + A4249_RS03485 0.35 +0.1 726,565 - A4249_RS03485 0.35 +1.4 726,565 - A4249_RS03485 0.35 +0.1 726,759 + A4249_RS03485 0.73 +0.2 726,759 + A4249_RS03485 0.73 +0.2 726,760 - A4249_RS03485 0.73 -1.3 726,897 + -1.0
Or see this region's nucleotide sequence