Experiment: Thymine 0.793 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS02490 and A4249_RS02495 overlap by 4 nucleotides A4249_RS02495 and A4249_RS02500 are separated by 52 nucleotides A4249_RS02500 and A4249_RS02505 are separated by 42 nucleotides
A4249_RS02490: A4249_RS02490 - methylmalonyl-CoA mutase, at 534,248 to 536,407
_RS02490
A4249_RS02495: A4249_RS02495 - methylmalonyl-CoA epimerase, at 536,404 to 536,826
_RS02495
A4249_RS02500: A4249_RS02500 - DUF4232 domain-containing protein, at 536,879 to 537,793
_RS02500
A4249_RS02505: A4249_RS02505 - glycine C-acetyltransferase, at 537,836 to 539,026
_RS02505
Position (kb)
536
537
538 Strain fitness (log2 ratio)
-2
-1
0
1 at 535.896 kb on + strand, within A4249_RS02490 at 535.896 kb on + strand, within A4249_RS02490 at 535.896 kb on + strand, within A4249_RS02490 at 535.896 kb on + strand, within A4249_RS02490 at 535.897 kb on - strand, within A4249_RS02490 at 535.897 kb on - strand, within A4249_RS02490 at 535.897 kb on - strand, within A4249_RS02490 at 535.898 kb on + strand, within A4249_RS02490 at 535.898 kb on + strand, within A4249_RS02490 at 535.898 kb on + strand, within A4249_RS02490 at 535.899 kb on - strand, within A4249_RS02490 at 535.899 kb on - strand, within A4249_RS02490 at 536.285 kb on - strand at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.465 kb on + strand, within A4249_RS02495 at 536.466 kb on - strand, within A4249_RS02495 at 536.466 kb on - strand, within A4249_RS02495 at 536.466 kb on - strand, within A4249_RS02495 at 536.466 kb on - strand, within A4249_RS02495 at 536.466 kb on - strand, within A4249_RS02495 at 536.466 kb on - strand, within A4249_RS02495 at 536.466 kb on - strand, within A4249_RS02495 at 536.466 kb on - strand, within A4249_RS02495 at 536.466 kb on - strand, within A4249_RS02495 at 536.467 kb on + strand, within A4249_RS02495 at 536.467 kb on + strand, within A4249_RS02495 at 536.548 kb on - strand, within A4249_RS02495 at 536.581 kb on + strand, within A4249_RS02495 at 536.581 kb on + strand, within A4249_RS02495 at 536.581 kb on + strand, within A4249_RS02495 at 536.582 kb on - strand, within A4249_RS02495 at 536.582 kb on - strand, within A4249_RS02495 at 536.582 kb on - strand, within A4249_RS02495 at 536.582 kb on - strand, within A4249_RS02495 at 536.582 kb on - strand, within A4249_RS02495 at 536.824 kb on + strand at 536.825 kb on - strand at 537.020 kb on + strand, within A4249_RS02500 at 537.021 kb on - strand, within A4249_RS02500 at 537.021 kb on - strand, within A4249_RS02500 at 537.056 kb on + strand, within A4249_RS02500 at 537.056 kb on + strand, within A4249_RS02500 at 537.056 kb on + strand, within A4249_RS02500 at 537.056 kb on + strand, within A4249_RS02500 at 537.056 kb on + strand, within A4249_RS02500 at 537.057 kb on - strand, within A4249_RS02500 at 537.057 kb on - strand, within A4249_RS02500 at 537.057 kb on - strand, within A4249_RS02500 at 537.057 kb on - strand, within A4249_RS02500 at 537.092 kb on - strand, within A4249_RS02500 at 537.092 kb on - strand, within A4249_RS02500 at 537.101 kb on + strand, within A4249_RS02500 at 537.101 kb on + strand, within A4249_RS02500 at 537.101 kb on + strand, within A4249_RS02500 at 537.101 kb on + strand, within A4249_RS02500 at 537.101 kb on + strand, within A4249_RS02500 at 537.101 kb on + strand, within A4249_RS02500 at 537.101 kb on + strand, within A4249_RS02500 at 537.101 kb on + strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.102 kb on - strand, within A4249_RS02500 at 537.103 kb on + strand, within A4249_RS02500 at 537.103 kb on + strand, within A4249_RS02500 at 537.103 kb on + strand, within A4249_RS02500 at 537.103 kb on + strand, within A4249_RS02500 at 537.103 kb on + strand, within A4249_RS02500 at 537.103 kb on + strand, within A4249_RS02500 at 537.103 kb on + strand, within A4249_RS02500 at 537.104 kb on - strand, within A4249_RS02500 at 537.104 kb on - strand, within A4249_RS02500 at 537.104 kb on - strand, within A4249_RS02500 at 537.104 kb on - strand, within A4249_RS02500 at 537.104 kb on - strand, within A4249_RS02500 at 537.104 kb on - strand, within A4249_RS02500 at 537.141 kb on - strand, within A4249_RS02500 at 537.141 kb on - strand, within A4249_RS02500 at 537.141 kb on - strand, within A4249_RS02500 at 537.358 kb on + strand, within A4249_RS02500 at 537.358 kb on + strand, within A4249_RS02500 at 537.358 kb on + strand, within A4249_RS02500 at 537.439 kb on + strand, within A4249_RS02500 at 537.545 kb on + strand, within A4249_RS02500 at 537.545 kb on + strand, within A4249_RS02500 at 537.545 kb on + strand, within A4249_RS02500 at 537.545 kb on + strand, within A4249_RS02500 at 537.545 kb on + strand, within A4249_RS02500 at 537.545 kb on + strand, within A4249_RS02500 at 537.545 kb on + strand, within A4249_RS02500 at 537.546 kb on - strand, within A4249_RS02500 at 537.546 kb on - strand, within A4249_RS02500 at 537.546 kb on - strand, within A4249_RS02500 at 537.546 kb on - strand, within A4249_RS02500 at 537.546 kb on - strand, within A4249_RS02500 at 537.546 kb on - strand, within A4249_RS02500 at 537.602 kb on + strand, within A4249_RS02500 at 537.602 kb on + strand, within A4249_RS02500 at 537.602 kb on + strand, within A4249_RS02500 at 537.602 kb on + strand, within A4249_RS02500 at 537.602 kb on + strand, within A4249_RS02500 at 537.602 kb on + strand, within A4249_RS02500 at 537.602 kb on + strand, within A4249_RS02500 at 537.602 kb on + strand, within A4249_RS02500 at 537.603 kb on - strand, within A4249_RS02500 at 537.603 kb on - strand, within A4249_RS02500 at 537.603 kb on - strand, within A4249_RS02500 at 537.673 kb on - strand, within A4249_RS02500 at 537.673 kb on - strand, within A4249_RS02500 at 537.823 kb on + strand at 537.823 kb on + strand at 537.823 kb on + strand at 537.851 kb on + strand at 537.851 kb on + strand at 537.851 kb on + strand at 537.851 kb on + strand at 537.851 kb on + strand at 537.852 kb on - strand at 537.852 kb on - strand at 537.986 kb on + strand, within A4249_RS02505 at 537.986 kb on + strand, within A4249_RS02505 at 537.986 kb on + strand, within A4249_RS02505 at 537.986 kb on + strand, within A4249_RS02505 at 537.986 kb on + strand, within A4249_RS02505 at 537.986 kb on + strand, within A4249_RS02505 at 537.986 kb on + strand, within A4249_RS02505 at 537.986 kb on + strand, within A4249_RS02505 at 537.986 kb on + strand, within A4249_RS02505 at 537.986 kb on + strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 537.987 kb on - strand, within A4249_RS02505 at 538.127 kb on + strand, within A4249_RS02505 at 538.128 kb on - strand, within A4249_RS02505 at 538.128 kb on - strand, within A4249_RS02505 at 538.128 kb on - strand, within A4249_RS02505 at 538.397 kb on + strand, within A4249_RS02505 at 538.397 kb on + strand, within A4249_RS02505 at 538.397 kb on + strand, within A4249_RS02505 at 538.397 kb on + strand, within A4249_RS02505 at 538.397 kb on + strand, within A4249_RS02505 at 538.398 kb on - strand, within A4249_RS02505 at 538.398 kb on - strand, within A4249_RS02505 at 538.398 kb on - strand, within A4249_RS02505 at 538.398 kb on - strand, within A4249_RS02505 at 538.398 kb on - strand, within A4249_RS02505 at 538.398 kb on - strand, within A4249_RS02505 at 538.400 kb on - strand, within A4249_RS02505 at 538.439 kb on + strand, within A4249_RS02505 at 538.439 kb on + strand, within A4249_RS02505 at 538.439 kb on + strand, within A4249_RS02505 at 538.439 kb on + strand, within A4249_RS02505 at 538.439 kb on + strand, within A4249_RS02505 at 538.440 kb on - strand, within A4249_RS02505 at 538.440 kb on - strand, within A4249_RS02505 at 538.514 kb on + strand, within A4249_RS02505 at 538.515 kb on - strand, within A4249_RS02505 at 538.637 kb on + strand, within A4249_RS02505 at 538.638 kb on - strand, within A4249_RS02505 at 538.638 kb on - strand, within A4249_RS02505 at 538.762 kb on + strand, within A4249_RS02505 at 538.763 kb on - strand, within A4249_RS02505
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine 0.793 mM (C) remove 535,896 + A4249_RS02490 0.76 -0.4 535,896 + A4249_RS02490 0.76 -0.3 535,896 + A4249_RS02490 0.76 +0.6 535,896 + A4249_RS02490 0.76 -0.4 535,897 - A4249_RS02490 0.76 +0.2 535,897 - A4249_RS02490 0.76 +0.4 535,897 - A4249_RS02490 0.76 -0.4 535,898 + A4249_RS02490 0.76 +0.6 535,898 + A4249_RS02490 0.76 -0.1 535,898 + A4249_RS02490 0.76 +0.5 535,899 - A4249_RS02490 0.76 -0.3 535,899 - A4249_RS02490 0.76 -1.0 536,285 - -0.5 536,465 + A4249_RS02495 0.14 +0.6 536,465 + A4249_RS02495 0.14 -0.8 536,465 + A4249_RS02495 0.14 +0.2 536,465 + A4249_RS02495 0.14 +0.1 536,465 + A4249_RS02495 0.14 -1.1 536,465 + A4249_RS02495 0.14 +1.0 536,465 + A4249_RS02495 0.14 +0.3 536,465 + A4249_RS02495 0.14 +0.3 536,465 + A4249_RS02495 0.14 +0.3 536,465 + A4249_RS02495 0.14 -1.0 536,465 + A4249_RS02495 0.14 -0.2 536,465 + A4249_RS02495 0.14 -0.2 536,465 + A4249_RS02495 0.14 +0.8 536,465 + A4249_RS02495 0.14 -0.6 536,466 - A4249_RS02495 0.15 +1.5 536,466 - A4249_RS02495 0.15 -0.2 536,466 - A4249_RS02495 0.15 +0.6 536,466 - A4249_RS02495 0.15 -0.1 536,466 - A4249_RS02495 0.15 +1.5 536,466 - A4249_RS02495 0.15 +0.3 536,466 - A4249_RS02495 0.15 -0.3 536,466 - A4249_RS02495 0.15 +0.2 536,466 - A4249_RS02495 0.15 -1.5 536,467 + A4249_RS02495 0.15 +0.2 536,467 + A4249_RS02495 0.15 -0.4 536,548 - A4249_RS02495 0.34 +0.1 536,581 + A4249_RS02495 0.42 +0.5 536,581 + A4249_RS02495 0.42 -0.5 536,581 + A4249_RS02495 0.42 -1.0 536,582 - A4249_RS02495 0.42 +0.4 536,582 - A4249_RS02495 0.42 +0.6 536,582 - A4249_RS02495 0.42 +0.2 536,582 - A4249_RS02495 0.42 -0.4 536,582 - A4249_RS02495 0.42 +1.2 536,824 + -0.0 536,825 - -0.6 537,020 + A4249_RS02500 0.15 +0.0 537,021 - A4249_RS02500 0.16 +0.2 537,021 - A4249_RS02500 0.16 +0.1 537,056 + A4249_RS02500 0.19 +0.2 537,056 + A4249_RS02500 0.19 +0.6 537,056 + A4249_RS02500 0.19 +0.0 537,056 + A4249_RS02500 0.19 +1.5 537,056 + A4249_RS02500 0.19 -0.8 537,057 - A4249_RS02500 0.19 -0.5 537,057 - A4249_RS02500 0.19 +0.3 537,057 - A4249_RS02500 0.19 +0.1 537,057 - A4249_RS02500 0.19 +0.5 537,092 - A4249_RS02500 0.23 +0.1 537,092 - A4249_RS02500 0.23 -1.8 537,101 + A4249_RS02500 0.24 +0.6 537,101 + A4249_RS02500 0.24 -1.1 537,101 + A4249_RS02500 0.24 -0.6 537,101 + A4249_RS02500 0.24 +0.1 537,101 + A4249_RS02500 0.24 -0.0 537,101 + A4249_RS02500 0.24 +0.7 537,101 + A4249_RS02500 0.24 -0.4 537,101 + A4249_RS02500 0.24 -1.0 537,102 - A4249_RS02500 0.24 -0.5 537,102 - A4249_RS02500 0.24 +1.4 537,102 - A4249_RS02500 0.24 -0.2 537,102 - A4249_RS02500 0.24 -0.1 537,102 - A4249_RS02500 0.24 -0.1 537,102 - A4249_RS02500 0.24 -0.2 537,102 - A4249_RS02500 0.24 +0.5 537,102 - A4249_RS02500 0.24 +0.4 537,102 - A4249_RS02500 0.24 +1.7 537,102 - A4249_RS02500 0.24 +0.7 537,102 - A4249_RS02500 0.24 -0.3 537,102 - A4249_RS02500 0.24 -0.2 537,103 + A4249_RS02500 0.24 -0.9 537,103 + A4249_RS02500 0.24 -0.2 537,103 + A4249_RS02500 0.24 +0.6 537,103 + A4249_RS02500 0.24 +0.4 537,103 + A4249_RS02500 0.24 +0.5 537,103 + A4249_RS02500 0.24 -0.2 537,103 + A4249_RS02500 0.24 +0.6 537,104 - A4249_RS02500 0.25 -1.0 537,104 - A4249_RS02500 0.25 +0.6 537,104 - A4249_RS02500 0.25 +1.1 537,104 - A4249_RS02500 0.25 +0.1 537,104 - A4249_RS02500 0.25 -0.5 537,104 - A4249_RS02500 0.25 -1.5 537,141 - A4249_RS02500 0.29 -0.2 537,141 - A4249_RS02500 0.29 -0.7 537,141 - A4249_RS02500 0.29 +0.4 537,358 + A4249_RS02500 0.52 +0.4 537,358 + A4249_RS02500 0.52 +0.5 537,358 + A4249_RS02500 0.52 -0.1 537,439 + A4249_RS02500 0.61 +0.2 537,545 + A4249_RS02500 0.73 -1.7 537,545 + A4249_RS02500 0.73 -0.2 537,545 + A4249_RS02500 0.73 +0.7 537,545 + A4249_RS02500 0.73 +0.1 537,545 + A4249_RS02500 0.73 +0.7 537,545 + A4249_RS02500 0.73 +0.0 537,545 + A4249_RS02500 0.73 -0.5 537,546 - A4249_RS02500 0.73 -0.5 537,546 - A4249_RS02500 0.73 +0.2 537,546 - A4249_RS02500 0.73 +0.1 537,546 - A4249_RS02500 0.73 -0.5 537,546 - A4249_RS02500 0.73 +0.3 537,546 - A4249_RS02500 0.73 +0.8 537,602 + A4249_RS02500 0.79 -0.0 537,602 + A4249_RS02500 0.79 +0.1 537,602 + A4249_RS02500 0.79 -0.2 537,602 + A4249_RS02500 0.79 -0.3 537,602 + A4249_RS02500 0.79 +0.6 537,602 + A4249_RS02500 0.79 +0.9 537,602 + A4249_RS02500 0.79 -0.0 537,602 + A4249_RS02500 0.79 +0.7 537,603 - A4249_RS02500 0.79 -1.9 537,603 - A4249_RS02500 0.79 +0.2 537,603 - A4249_RS02500 0.79 +0.1 537,673 - A4249_RS02500 0.87 -0.0 537,673 - A4249_RS02500 0.87 -0.0 537,823 + -0.2 537,823 + -0.1 537,823 + +0.3 537,851 + -0.6 537,851 + +0.1 537,851 + +0.2 537,851 + -0.2 537,851 + +0.4 537,852 - +0.6 537,852 - +0.0 537,986 + A4249_RS02505 0.13 +0.5 537,986 + A4249_RS02505 0.13 +0.2 537,986 + A4249_RS02505 0.13 -0.4 537,986 + A4249_RS02505 0.13 -0.5 537,986 + A4249_RS02505 0.13 +0.3 537,986 + A4249_RS02505 0.13 +0.3 537,986 + A4249_RS02505 0.13 +0.2 537,986 + A4249_RS02505 0.13 -0.0 537,986 + A4249_RS02505 0.13 -0.1 537,986 + A4249_RS02505 0.13 +0.2 537,987 - A4249_RS02505 0.13 -0.0 537,987 - A4249_RS02505 0.13 +0.1 537,987 - A4249_RS02505 0.13 -2.0 537,987 - A4249_RS02505 0.13 +0.5 537,987 - A4249_RS02505 0.13 +0.4 537,987 - A4249_RS02505 0.13 -0.5 537,987 - A4249_RS02505 0.13 +0.3 537,987 - A4249_RS02505 0.13 -0.5 537,987 - A4249_RS02505 0.13 -0.9 537,987 - A4249_RS02505 0.13 +0.6 537,987 - A4249_RS02505 0.13 +0.5 537,987 - A4249_RS02505 0.13 +0.1 537,987 - A4249_RS02505 0.13 +0.1 537,987 - A4249_RS02505 0.13 -0.1 538,127 + A4249_RS02505 0.24 -1.7 538,128 - A4249_RS02505 0.25 +0.5 538,128 - A4249_RS02505 0.25 -0.2 538,128 - A4249_RS02505 0.25 -0.5 538,397 + A4249_RS02505 0.47 -0.5 538,397 + A4249_RS02505 0.47 +0.8 538,397 + A4249_RS02505 0.47 -0.7 538,397 + A4249_RS02505 0.47 -0.1 538,397 + A4249_RS02505 0.47 -0.2 538,398 - A4249_RS02505 0.47 -1.3 538,398 - A4249_RS02505 0.47 -0.1 538,398 - A4249_RS02505 0.47 +0.5 538,398 - A4249_RS02505 0.47 -0.5 538,398 - A4249_RS02505 0.47 +0.3 538,398 - A4249_RS02505 0.47 -0.2 538,400 - A4249_RS02505 0.47 -0.2 538,439 + A4249_RS02505 0.51 +0.1 538,439 + A4249_RS02505 0.51 -0.3 538,439 + A4249_RS02505 0.51 +0.1 538,439 + A4249_RS02505 0.51 +0.6 538,439 + A4249_RS02505 0.51 -0.2 538,440 - A4249_RS02505 0.51 -1.3 538,440 - A4249_RS02505 0.51 -1.0 538,514 + A4249_RS02505 0.57 +0.9 538,515 - A4249_RS02505 0.57 +0.1 538,637 + A4249_RS02505 0.67 -0.1 538,638 - A4249_RS02505 0.67 -0.3 538,638 - A4249_RS02505 0.67 -0.3 538,762 + A4249_RS02505 0.78 +0.1 538,763 - A4249_RS02505 0.78 -0.6
Or see this region's nucleotide sequence