Experiment: Thymine 0.793 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS00945 and A4249_RS00950 are separated by 64 nucleotides A4249_RS00950 and A4249_RS00955 are separated by 3 nucleotides
A4249_RS00945: A4249_RS00945 - electron transfer flavoprotein-ubiquinone oxidoreductase, at 174,271 to 175,926
_RS00945
A4249_RS00950: A4249_RS00950 - tetratricopeptide repeat protein, at 175,991 to 177,868
_RS00950
A4249_RS00955: A4249_RS00955 - 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase, at 177,872 to 178,750
_RS00955
Position (kb) 175
176
177
178 Strain fitness (log2 ratio) -1
0
1
2
3 at 176.291 kb on + strand, within A4249_RS00950 at 176.291 kb on + strand, within A4249_RS00950 at 176.291 kb on + strand, within A4249_RS00950 at 176.757 kb on + strand, within A4249_RS00950 at 176.757 kb on + strand, within A4249_RS00950 at 176.757 kb on + strand, within A4249_RS00950 at 176.757 kb on + strand, within A4249_RS00950 at 176.758 kb on - strand, within A4249_RS00950 at 176.758 kb on - strand, within A4249_RS00950 at 176.858 kb on + strand, within A4249_RS00950 at 176.858 kb on + strand, within A4249_RS00950 at 177.035 kb on + strand, within A4249_RS00950 at 177.035 kb on + strand, within A4249_RS00950 at 177.035 kb on + strand, within A4249_RS00950 at 177.080 kb on + strand, within A4249_RS00950 at 177.080 kb on + strand, within A4249_RS00950 at 177.170 kb on + strand, within A4249_RS00950 at 177.410 kb on + strand, within A4249_RS00950 at 177.410 kb on + strand, within A4249_RS00950 at 177.410 kb on + strand, within A4249_RS00950 at 177.410 kb on + strand, within A4249_RS00950 at 177.497 kb on + strand, within A4249_RS00950 at 177.497 kb on + strand, within A4249_RS00950 at 177.497 kb on + strand, within A4249_RS00950 at 177.635 kb on + strand, within A4249_RS00950 at 177.635 kb on + strand, within A4249_RS00950 at 177.719 kb on + strand at 177.866 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine 0.793 mM (C) remove 176,291 + A4249_RS00950 0.16 +0.4 176,291 + A4249_RS00950 0.16 +0.3 176,291 + A4249_RS00950 0.16 -0.3 176,757 + A4249_RS00950 0.41 +0.1 176,757 + A4249_RS00950 0.41 -0.5 176,757 + A4249_RS00950 0.41 -0.2 176,757 + A4249_RS00950 0.41 -1.6 176,758 - A4249_RS00950 0.41 -0.7 176,758 - A4249_RS00950 0.41 +0.3 176,858 + A4249_RS00950 0.46 -0.4 176,858 + A4249_RS00950 0.46 -0.2 177,035 + A4249_RS00950 0.56 -0.4 177,035 + A4249_RS00950 0.56 +0.2 177,035 + A4249_RS00950 0.56 +3.1 177,080 + A4249_RS00950 0.58 -0.2 177,080 + A4249_RS00950 0.58 +0.3 177,170 + A4249_RS00950 0.63 -0.6 177,410 + A4249_RS00950 0.76 -1.3 177,410 + A4249_RS00950 0.76 -0.6 177,410 + A4249_RS00950 0.76 +0.8 177,410 + A4249_RS00950 0.76 -0.5 177,497 + A4249_RS00950 0.80 +0.4 177,497 + A4249_RS00950 0.80 -1.6 177,497 + A4249_RS00950 0.80 -0.6 177,635 + A4249_RS00950 0.88 -0.3 177,635 + A4249_RS00950 0.88 -1.2 177,719 + +0.3 177,866 + +0.5
Or see this region's nucleotide sequence