Strain Fitness in Escherichia coli ECRC101 around MCAODC_26385

Experiment: WM_Bas09

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	WM_Bas09
1,998,294	+	nagA	MCAODC_26380	0.24	-1.0
1,998,297	+	nagA	MCAODC_26380	0.24	+0.5
1,998,297	+	nagA	MCAODC_26380	0.24	-0.2
1,998,297	+	nagA	MCAODC_26380	0.24	-2.4
1,998,297	+	nagA	MCAODC_26380	0.24	-2.3
1,998,301	+	nagA	MCAODC_26380	0.25	-2.0
1,998,367	+	nagA	MCAODC_26380	0.30	-1.6
1,998,367	+	nagA	MCAODC_26380	0.30	-2.2
1,998,447	+	nagA	MCAODC_26380	0.37	-3.1
1,998,447	+	nagA	MCAODC_26380	0.37	-3.3
1,998,670	+	nagA	MCAODC_26380	0.57	-2.7
1,998,670	+	nagA	MCAODC_26380	0.57	-2.7
1,998,685	+	nagA	MCAODC_26380	0.58	-2.3
1,998,686	-	nagA	MCAODC_26380	0.58	-3.3
1,998,687	+	nagA	MCAODC_26380	0.58	-3.4
1,998,688	-	nagA	MCAODC_26380	0.58	-2.3
1,998,742	+	nagA	MCAODC_26380	0.63	-1.8
1,998,742	+	nagA	MCAODC_26380	0.63	-0.4
1,998,742	+	nagA	MCAODC_26380	0.63	-2.6
1,998,754	-	nagA	MCAODC_26380	0.64	-3.3
1,998,893	+	nagA	MCAODC_26380	0.76	-2.5
1,998,893	+	nagA	MCAODC_26380	0.76	-2.5
1,998,893	+	nagA	MCAODC_26380	0.76	-1.9
1,998,895	+	nagA	MCAODC_26380	0.76	-2.7
1,998,895	+	nagA	MCAODC_26380	0.76	-3.7
1,998,895	+	nagA	MCAODC_26380	0.76	-2.4
1,998,895	+	nagA	MCAODC_26380	0.76	+1.3
1,998,895	+	nagA	MCAODC_26380	0.76	-2.5
1,998,898	+	nagA	MCAODC_26380	0.77	-3.5
1,998,898	+	nagA	MCAODC_26380	0.77	-0.1
1,998,930	+	nagA	MCAODC_26380	0.79	-4.0
1,999,083	+				-0.9
1,999,158	-				+1.2
1,999,175	+				+0.3
1,999,176	-				-1.1
1,999,176	-				-1.9
1,999,201	+				-0.7
1,999,205	+				-2.5
1,999,205	+				-2.2
1,999,206	-				+0.9
1,999,229	+				-2.5
1,999,229	+				+0.7
1,999,229	+				-1.1
1,999,230	-				-0.9
1,999,589	+	nagC	MCAODC_26385	0.34	+1.7
1,999,593	+	nagC	MCAODC_26385	0.34	-0.1
1,999,594	-	nagC	MCAODC_26385	0.34	+1.4
1,999,594	-	nagC	MCAODC_26385	0.34	+0.3
1,999,711	+	nagC	MCAODC_26385	0.44	+1.2
1,999,788	+	nagC	MCAODC_26385	0.50	-0.0
1,999,788	+	nagC	MCAODC_26385	0.50	+2.0
2,000,031	+	nagC	MCAODC_26385	0.70	-2.7
2,000,072	+	nagC	MCAODC_26385	0.73	-1.3
2,000,124	+	nagC	MCAODC_26385	0.78	+0.5
2,000,125	-	nagC	MCAODC_26385	0.78	+0.3
2,000,125	-	nagC	MCAODC_26385	0.78	-0.5
2,000,219	+	nagC	MCAODC_26385	0.85	-1.2
2,000,252	+	nagC	MCAODC_26385	0.88	+0.1
2,000,252	+	nagC	MCAODC_26385	0.88	+0.6
2,000,252	+	nagC	MCAODC_26385	0.88	-0.8
2,000,255	+	nagC	MCAODC_26385	0.88	+0.2
2,000,255	+	nagC	MCAODC_26385	0.88	-0.2
2,000,256	-	nagC	MCAODC_26385	0.88	+0.5
2,000,279	+				+1.4
2,000,280	-				-1.2
2,000,282	+				-1.2
2,000,283	-				-0.3
2,000,283	-				+1.5
2,000,367	-				-1.8
2,000,405	+				-1.1
2,000,407	+				+1.2
2,000,460	+				+0.5
2,000,573	+	nagD	MCAODC_26390	0.17	+1.1
2,000,632	-	nagD	MCAODC_26390	0.25	+1.4
2,000,648	+	nagD	MCAODC_26390	0.27	-1.0
2,000,649	-	nagD	MCAODC_26390	0.27	-0.8
2,000,650	+	nagD	MCAODC_26390	0.27	+0.9
2,000,650	+	nagD	MCAODC_26390	0.27	+0.1
2,000,651	-	nagD	MCAODC_26390	0.27	+0.0
2,000,651	-	nagD	MCAODC_26390	0.27	-0.2
2,000,705	+	nagD	MCAODC_26390	0.35	-0.3
2,000,705	+	nagD	MCAODC_26390	0.35	+1.6
2,000,705	+	nagD	MCAODC_26390	0.35	-0.2
2,000,705	+	nagD	MCAODC_26390	0.35	+2.8
2,000,706	-	nagD	MCAODC_26390	0.35	-0.8
2,000,706	-	nagD	MCAODC_26390	0.35	+0.6
2,000,706	-	nagD	MCAODC_26390	0.35	+0.1
2,000,741	+	nagD	MCAODC_26390	0.39	+0.0
2,000,741	+	nagD	MCAODC_26390	0.39	-0.0
2,000,742	-	nagD	MCAODC_26390	0.40	-0.8
2,000,758	+	nagD	MCAODC_26390	0.42	+1.4
2,000,807	+	nagD	MCAODC_26390	0.48	-0.3
2,000,807	+	nagD	MCAODC_26390	0.48	+0.9
2,000,807	+	nagD	MCAODC_26390	0.48	-3.6
2,000,808	-	nagD	MCAODC_26390	0.48	+0.0
2,000,808	-	nagD	MCAODC_26390	0.48	+1.1
2,000,808	-	nagD	MCAODC_26390	0.48	+0.3
2,000,827	+	nagD	MCAODC_26390	0.51	-2.0
2,000,827	+	nagD	MCAODC_26390	0.51	-3.0
2,000,828	-	nagD	MCAODC_26390	0.51	+0.7
2,000,828	-	nagD	MCAODC_26390	0.51	-0.5
2,000,864	-	nagD	MCAODC_26390	0.56	-0.1
2,000,864	-	nagD	MCAODC_26390	0.56	-0.8
2,000,960	+	nagD	MCAODC_26390	0.69	+1.2
2,000,960	+	nagD	MCAODC_26390	0.69	+3.7
2,000,960	+	nagD	MCAODC_26390	0.69	+0.1
2,000,960	+	nagD	MCAODC_26390	0.69	-0.5
2,000,960	+	nagD	MCAODC_26390	0.69	+1.1
2,000,960	+	nagD	MCAODC_26390	0.69	-0.7
2,000,960	+	nagD	MCAODC_26390	0.69	-0.9
2,000,961	-	nagD	MCAODC_26390	0.69	+0.6
2,000,961	-	nagD	MCAODC_26390	0.69	-1.7
2,000,961	-	nagD	MCAODC_26390	0.69	-0.8
2,000,961	-	nagD	MCAODC_26390	0.69	-0.9
2,001,139	+				+1.1
2,001,139	+				-0.8
2,001,139	+				-0.5
2,001,139	+				-0.0
2,001,140	-				+0.7
2,001,190	+				-0.1
2,001,198	+				+0.1
2,001,198	+				-0.2
2,001,198	+				-0.4
2,001,228	-				+0.0