Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_24325 and tnpB are separated by 49 nucleotides tnpB and tnpB overlap by 4 nucleotides tnpB and nu1 are separated by 75 nucleotides nu1 and MCAODC_24345 are separated by 750 nucleotides
MCAODC_24325: MCAODC_24325 - IS66-like element ISEc8 family transposase, at 1,590,701 to 1,592,239
_24325
MCAODC_24330: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 1,592,289 to 1,592,636
tnpB
MCAODC_24335: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 1,592,633 to 1,593,013
tnpB
MCAODC_24340: nu1 - Prophage Qin DNA packaging protein NU1-like protein, at 1,593,089 to 1,593,364
nu1
MCAODC_24345: MCAODC_24345 - holin, at 1,594,115 to 1,594,321
_24345
Position (kb) 1593
1594 Strain fitness (log2 ratio) -1
0
1
2 at 1593.097 kb on + strand at 1593.799 kb on - strand at 1593.810 kb on - strand at 1593.810 kb on - strand at 1593.939 kb on + strand at 1593.939 kb on + strand at 1593.951 kb on + strand at 1593.951 kb on + strand at 1594.319 kb on + strand at 1594.350 kb on + strand at 1594.351 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 1,593,097 + -0.8 1,593,799 - +0.2 1,593,810 - +0.3 1,593,810 - -0.9 1,593,939 + +1.3 1,593,939 + +0.2 1,593,951 + +2.2 1,593,951 + -0.5 1,594,319 + +0.6 1,594,350 + -0.7 1,594,351 - -1.5
Or see this region's nucleotide sequence