Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydeI and ydeJ are separated by 275 nucleotides ydeJ and dcp are separated by 44 nucleotides dcp and ydfG are separated by 136 nucleotides ydfG and ydfH are separated by 88 nucleotides
MCAODC_16895: ydeI - YdeI family stress tolerance OB fold protein, at 287,573 to 287,965
ydeI
MCAODC_16900: ydeJ - 2-oxo-tetronate isomerase, at 288,241 to 288,759
ydeJ
MCAODC_16905: dcp - peptidyl-dipeptidase Dcp, at 288,804 to 290,849
dcp
MCAODC_16910: ydfG - bifunctional NADP-dependent 3-hydroxy acid dehydrogenase/3-hydroxypropionate dehydrogenase YdfG, at 290,986 to 291,732
ydfG
MCAODC_16915: ydfH - DNA-binding transcriptional regulator YdfH, at 291,821 to 292,507
ydfH
Position (kb) 288
289
290
291 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 288.076 kb on - strand at 288.214 kb on + strand at 288.214 kb on + strand at 288.338 kb on + strand, within ydeJ at 288.345 kb on - strand, within ydeJ at 288.349 kb on - strand, within ydeJ at 288.473 kb on + strand, within ydeJ at 288.664 kb on + strand, within ydeJ at 288.664 kb on + strand, within ydeJ at 288.704 kb on + strand, within ydeJ at 288.708 kb on - strand at 288.708 kb on - strand at 288.708 kb on - strand at 288.755 kb on + strand at 288.756 kb on - strand at 288.806 kb on - strand at 288.806 kb on - strand at 288.806 kb on - strand at 288.808 kb on - strand at 288.840 kb on - strand at 289.013 kb on - strand, within dcp at 289.016 kb on - strand, within dcp at 289.452 kb on + strand, within dcp at 289.470 kb on + strand, within dcp at 289.710 kb on - strand, within dcp at 289.893 kb on + strand, within dcp at 290.030 kb on + strand, within dcp at 290.031 kb on - strand, within dcp at 290.531 kb on - strand, within dcp at 290.535 kb on - strand, within dcp at 290.535 kb on - strand, within dcp at 290.535 kb on - strand, within dcp at 290.535 kb on - strand, within dcp at 290.586 kb on + strand, within dcp at 290.601 kb on - strand, within dcp at 290.676 kb on - strand at 290.678 kb on + strand at 290.678 kb on + strand at 290.837 kb on + strand at 290.883 kb on + strand at 290.925 kb on - strand at 290.949 kb on - strand at 290.949 kb on - strand at 290.999 kb on + strand at 291.123 kb on + strand, within ydfG at 291.207 kb on + strand, within ydfG at 291.208 kb on - strand, within ydfG at 291.317 kb on + strand, within ydfG at 291.318 kb on - strand, within ydfG at 291.319 kb on + strand, within ydfG at 291.319 kb on + strand, within ydfG at 291.319 kb on + strand, within ydfG at 291.320 kb on - strand, within ydfG at 291.320 kb on - strand, within ydfG at 291.321 kb on + strand, within ydfG at 291.321 kb on + strand, within ydfG at 291.358 kb on - strand, within ydfG at 291.358 kb on - strand, within ydfG at 291.369 kb on + strand, within ydfG at 291.372 kb on + strand, within ydfG at 291.372 kb on + strand, within ydfG at 291.372 kb on + strand, within ydfG at 291.373 kb on - strand, within ydfG at 291.703 kb on + strand at 291.737 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 288,076 - +1.3 288,214 + +0.0 288,214 + -2.2 288,338 + ydeJ MCAODC_16900 0.19 +0.8 288,345 - ydeJ MCAODC_16900 0.20 +1.7 288,349 - ydeJ MCAODC_16900 0.21 +0.3 288,473 + ydeJ MCAODC_16900 0.45 +0.1 288,664 + ydeJ MCAODC_16900 0.82 -1.5 288,664 + ydeJ MCAODC_16900 0.82 -2.5 288,704 + ydeJ MCAODC_16900 0.89 +1.6 288,708 - -0.9 288,708 - +1.5 288,708 - +1.1 288,755 + +0.6 288,756 - -1.1 288,806 - -0.5 288,806 - +0.7 288,806 - -3.8 288,808 - +0.3 288,840 - +3.0 289,013 - dcp MCAODC_16905 0.10 +0.1 289,016 - dcp MCAODC_16905 0.10 +0.7 289,452 + dcp MCAODC_16905 0.32 -1.3 289,470 + dcp MCAODC_16905 0.33 +0.1 289,710 - dcp MCAODC_16905 0.44 +0.6 289,893 + dcp MCAODC_16905 0.53 -0.3 290,030 + dcp MCAODC_16905 0.60 -1.2 290,031 - dcp MCAODC_16905 0.60 -1.9 290,531 - dcp MCAODC_16905 0.84 +0.1 290,535 - dcp MCAODC_16905 0.85 +1.6 290,535 - dcp MCAODC_16905 0.85 +1.7 290,535 - dcp MCAODC_16905 0.85 +2.9 290,535 - dcp MCAODC_16905 0.85 -0.6 290,586 + dcp MCAODC_16905 0.87 -0.3 290,601 - dcp MCAODC_16905 0.88 +2.7 290,676 - -2.7 290,678 + +1.7 290,678 + +0.6 290,837 + -2.4 290,883 + -0.3 290,925 - +0.9 290,949 - -0.4 290,949 - +0.5 290,999 + +2.0 291,123 + ydfG MCAODC_16910 0.18 +0.5 291,207 + ydfG MCAODC_16910 0.30 +0.4 291,208 - ydfG MCAODC_16910 0.30 -0.6 291,317 + ydfG MCAODC_16910 0.44 -0.3 291,318 - ydfG MCAODC_16910 0.44 +1.3 291,319 + ydfG MCAODC_16910 0.45 -1.2 291,319 + ydfG MCAODC_16910 0.45 +1.0 291,319 + ydfG MCAODC_16910 0.45 +2.0 291,320 - ydfG MCAODC_16910 0.45 -1.4 291,320 - ydfG MCAODC_16910 0.45 +0.1 291,321 + ydfG MCAODC_16910 0.45 -0.1 291,321 + ydfG MCAODC_16910 0.45 -1.8 291,358 - ydfG MCAODC_16910 0.50 -1.4 291,358 - ydfG MCAODC_16910 0.50 +1.1 291,369 + ydfG MCAODC_16910 0.51 +0.7 291,372 + ydfG MCAODC_16910 0.52 -2.2 291,372 + ydfG MCAODC_16910 0.52 -1.3 291,372 + ydfG MCAODC_16910 0.52 -2.5 291,373 - ydfG MCAODC_16910 0.52 -0.7 291,703 + +1.0 291,737 + +2.1
Or see this region's nucleotide sequence