Strain Fitness in Escherichia coli ECRC101 around MCAODC_10305

Experiment: WM_Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_10290 and MCAODC_10295 overlap by 23 nucleotidesMCAODC_10295 and MCAODC_10300 are separated by 72 nucleotidesMCAODC_10300 and fliN overlap by 41 nucleotidesfliN and escR overlap by 11 nucleotidesescR and MCAODC_10315 are separated by 10 nucleotidesMCAODC_10315 and sctT are separated by 1 nucleotides MCAODC_10290: MCAODC_10290 - type III secretion protein, at 2,056,880 to 2,057,338 _10290 MCAODC_10295: MCAODC_10295 - Type III secretion protein, at 2,057,316 to 2,057,549 _10295 MCAODC_10300: MCAODC_10300 - type III secretion protein, at 2,057,622 to 2,058,191 _10300 MCAODC_10305: fliN - YscQ/HrcQ family type III secretion apparatus protein, at 2,058,151 to 2,059,137 fliN MCAODC_10310: escR - EscR/YscR/HrcR family type III secretion system export apparatus protein, at 2,059,127 to 2,059,792 escR MCAODC_10315: MCAODC_10315 - EscS/YscS/HrcS family type III secretion system export apparatus protein, at 2,059,803 to 2,060,063 _10315 MCAODC_10320: sctT - type III secretion system export apparatus subunit SctT, at 2,060,065 to 2,060,517 sctT Position (kb) 2058 2059 2060Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2057.171 kb on - strand, within MCAODC_10290at 2057.315 kb on - strandat 2057.465 kb on - strand, within MCAODC_10295at 2057.646 kb on - strandat 2057.780 kb on - strand, within MCAODC_10300at 2058.010 kb on + strand, within MCAODC_10300at 2058.017 kb on - strand, within MCAODC_10300at 2058.074 kb on + strand, within MCAODC_10300at 2058.075 kb on - strand, within MCAODC_10300at 2058.279 kb on - strand, within fliNat 2058.282 kb on - strand, within fliNat 2058.300 kb on + strand, within fliNat 2058.546 kb on - strand, within fliNat 2058.897 kb on + strand, within fliNat 2059.050 kb on - strandat 2059.315 kb on - strand, within escRat 2059.315 kb on - strand, within escRat 2059.659 kb on + strand, within escRat 2059.660 kb on - strand, within escRat 2059.663 kb on + strand, within escRat 2059.664 kb on - strand, within escRat 2059.664 kb on - strand, within escRat 2059.853 kb on + strand, within MCAODC_10315at 2059.854 kb on - strand, within MCAODC_10315

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas09
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2,057,171 - MCAODC_10290 0.63 +1.6
2,057,315 - +1.5
2,057,465 - MCAODC_10295 0.64 -1.9
2,057,646 - +0.8
2,057,780 - MCAODC_10300 0.28 +0.5
2,058,010 + MCAODC_10300 0.68 +0.2
2,058,017 - MCAODC_10300 0.69 -0.6
2,058,074 + MCAODC_10300 0.79 -0.0
2,058,075 - MCAODC_10300 0.79 +0.4
2,058,279 - fliN MCAODC_10305 0.13 -0.8
2,058,282 - fliN MCAODC_10305 0.13 -1.9
2,058,300 + fliN MCAODC_10305 0.15 -2.8
2,058,546 - fliN MCAODC_10305 0.40 -0.5
2,058,897 + fliN MCAODC_10305 0.76 -0.2
2,059,050 - -1.4
2,059,315 - escR MCAODC_10310 0.28 +0.6
2,059,315 - escR MCAODC_10310 0.28 -1.4
2,059,659 + escR MCAODC_10310 0.80 +0.2
2,059,660 - escR MCAODC_10310 0.80 +0.3
2,059,663 + escR MCAODC_10310 0.80 +1.3
2,059,664 - escR MCAODC_10310 0.81 +1.9
2,059,664 - escR MCAODC_10310 0.81 -1.5
2,059,853 + MCAODC_10315 0.19 +0.6
2,059,854 - MCAODC_10315 0.20 +1.0

Or see this region's nucleotide sequence