Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_10290 and MCAODC_10295 overlap by 23 nucleotides MCAODC_10295 and MCAODC_10300 are separated by 72 nucleotides MCAODC_10300 and fliN overlap by 41 nucleotides fliN and escR overlap by 11 nucleotides escR and MCAODC_10315 are separated by 10 nucleotides MCAODC_10315 and sctT are separated by 1 nucleotides
MCAODC_10290: MCAODC_10290 - type III secretion protein, at 2,056,880 to 2,057,338
_10290
MCAODC_10295: MCAODC_10295 - Type III secretion protein, at 2,057,316 to 2,057,549
_10295
MCAODC_10300: MCAODC_10300 - type III secretion protein, at 2,057,622 to 2,058,191
_10300
MCAODC_10305: fliN - YscQ/HrcQ family type III secretion apparatus protein, at 2,058,151 to 2,059,137
fliN
MCAODC_10310: escR - EscR/YscR/HrcR family type III secretion system export apparatus protein, at 2,059,127 to 2,059,792
escR
MCAODC_10315: MCAODC_10315 - EscS/YscS/HrcS family type III secretion system export apparatus protein, at 2,059,803 to 2,060,063
_10315
MCAODC_10320: sctT - type III secretion system export apparatus subunit SctT, at 2,060,065 to 2,060,517
sctT
Position (kb)
2058
2059
2060 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2057.171 kb on - strand, within MCAODC_10290 at 2057.315 kb on - strand at 2057.465 kb on - strand, within MCAODC_10295 at 2057.646 kb on - strand at 2057.780 kb on - strand, within MCAODC_10300 at 2058.010 kb on + strand, within MCAODC_10300 at 2058.017 kb on - strand, within MCAODC_10300 at 2058.074 kb on + strand, within MCAODC_10300 at 2058.075 kb on - strand, within MCAODC_10300 at 2058.279 kb on - strand, within fliN at 2058.282 kb on - strand, within fliN at 2058.300 kb on + strand, within fliN at 2058.546 kb on - strand, within fliN at 2058.897 kb on + strand, within fliN at 2059.050 kb on - strand at 2059.315 kb on - strand, within escR at 2059.315 kb on - strand, within escR at 2059.659 kb on + strand, within escR at 2059.660 kb on - strand, within escR at 2059.663 kb on + strand, within escR at 2059.664 kb on - strand, within escR at 2059.664 kb on - strand, within escR at 2059.853 kb on + strand, within MCAODC_10315 at 2059.854 kb on - strand, within MCAODC_10315
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 2,057,171 - MCAODC_10290 0.63 +1.6 2,057,315 - +1.5 2,057,465 - MCAODC_10295 0.64 -1.9 2,057,646 - +0.8 2,057,780 - MCAODC_10300 0.28 +0.5 2,058,010 + MCAODC_10300 0.68 +0.2 2,058,017 - MCAODC_10300 0.69 -0.6 2,058,074 + MCAODC_10300 0.79 -0.0 2,058,075 - MCAODC_10300 0.79 +0.4 2,058,279 - fliN MCAODC_10305 0.13 -0.8 2,058,282 - fliN MCAODC_10305 0.13 -1.9 2,058,300 + fliN MCAODC_10305 0.15 -2.8 2,058,546 - fliN MCAODC_10305 0.40 -0.5 2,058,897 + fliN MCAODC_10305 0.76 -0.2 2,059,050 - -1.4 2,059,315 - escR MCAODC_10310 0.28 +0.6 2,059,315 - escR MCAODC_10310 0.28 -1.4 2,059,659 + escR MCAODC_10310 0.80 +0.2 2,059,660 - escR MCAODC_10310 0.80 +0.3 2,059,663 + escR MCAODC_10310 0.80 +1.3 2,059,664 - escR MCAODC_10310 0.81 +1.9 2,059,664 - escR MCAODC_10310 0.81 -1.5 2,059,853 + MCAODC_10315 0.19 +0.6 2,059,854 - MCAODC_10315 0.20 +1.0
Or see this region's nucleotide sequence