Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rbsB and ydaE are separated by 54 nucleotides ydaE and fba are separated by 14 nucleotides fba and rbsK overlap by 4 nucleotides
MCAODC_03230: rbsB - transcriptional regulator, at 650,236 to 651,177
rbsB
MCAODC_03235: ydaE - D-lyxose/D-mannose isomerase, at 651,232 to 651,915
ydaE
MCAODC_03240: fba - ketose 1,6-bisphosphate aldolase, at 651,930 to 652,790
fba
MCAODC_03245: rbsK - sugar kinase, at 652,787 to 653,788
rbsK
Position (kb) 651
652 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 650.331 kb on - strand, within rbsB at 650.444 kb on - strand, within rbsB at 650.471 kb on + strand, within rbsB at 650.472 kb on - strand, within rbsB at 650.472 kb on - strand, within rbsB at 650.505 kb on + strand, within rbsB at 650.592 kb on - strand, within rbsB at 650.655 kb on + strand, within rbsB at 650.655 kb on + strand, within rbsB at 650.656 kb on - strand, within rbsB at 650.656 kb on - strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.756 kb on - strand, within rbsB at 650.834 kb on + strand, within rbsB at 650.834 kb on + strand, within rbsB at 650.834 kb on + strand, within rbsB at 650.835 kb on - strand, within rbsB at 650.876 kb on + strand, within rbsB at 650.876 kb on + strand, within rbsB at 650.938 kb on - strand, within rbsB at 650.949 kb on + strand, within rbsB at 651.154 kb on + strand at 651.155 kb on - strand at 651.155 kb on - strand at 651.175 kb on + strand at 651.175 kb on + strand at 651.176 kb on - strand at 651.246 kb on + strand at 651.247 kb on - strand at 651.248 kb on + strand at 651.248 kb on + strand at 651.267 kb on + strand at 651.267 kb on + strand at 651.268 kb on - strand at 651.296 kb on + strand at 651.296 kb on + strand at 651.297 kb on - strand at 651.297 kb on - strand at 651.297 kb on - strand at 651.444 kb on + strand, within ydaE at 651.478 kb on + strand, within ydaE at 651.479 kb on - strand, within ydaE at 651.479 kb on - strand, within ydaE at 651.479 kb on - strand, within ydaE at 651.552 kb on + strand, within ydaE at 651.552 kb on + strand, within ydaE at 651.585 kb on + strand, within ydaE at 651.586 kb on - strand, within ydaE at 651.591 kb on - strand, within ydaE at 651.591 kb on - strand, within ydaE at 651.618 kb on + strand, within ydaE at 651.618 kb on + strand, within ydaE at 651.619 kb on - strand, within ydaE at 651.656 kb on + strand, within ydaE at 651.656 kb on + strand, within ydaE at 651.656 kb on + strand, within ydaE at 651.656 kb on + strand, within ydaE at 651.657 kb on - strand, within ydaE at 651.678 kb on + strand, within ydaE at 651.679 kb on - strand, within ydaE at 651.679 kb on - strand, within ydaE at 651.679 kb on - strand, within ydaE at 651.717 kb on + strand, within ydaE at 651.717 kb on + strand, within ydaE at 651.718 kb on - strand, within ydaE at 651.739 kb on + strand, within ydaE at 651.740 kb on - strand, within ydaE at 651.848 kb on - strand at 651.884 kb on + strand at 651.885 kb on - strand at 651.930 kb on - strand at 651.982 kb on - strand at 652.064 kb on + strand, within fba at 652.064 kb on + strand, within fba at 652.084 kb on + strand, within fba at 652.085 kb on - strand, within fba at 652.085 kb on - strand, within fba at 652.091 kb on + strand, within fba at 652.095 kb on + strand, within fba at 652.095 kb on + strand, within fba at 652.097 kb on + strand, within fba at 652.097 kb on + strand, within fba at 652.165 kb on + strand, within fba at 652.166 kb on - strand, within fba at 652.257 kb on + strand, within fba at 652.369 kb on - strand, within fba at 652.369 kb on - strand, within fba at 652.480 kb on - strand, within fba at 652.549 kb on + strand, within fba at 652.549 kb on + strand, within fba at 652.550 kb on - strand, within fba at 652.552 kb on + strand, within fba at 652.552 kb on + strand, within fba at 652.553 kb on - strand, within fba at 652.553 kb on - strand, within fba at 652.571 kb on + strand, within fba at 652.626 kb on + strand, within fba at 652.626 kb on + strand, within fba at 652.692 kb on + strand, within fba at 652.693 kb on - strand, within fba at 652.798 kb on + strand at 652.822 kb on + strand at 652.822 kb on + strand at 652.823 kb on - strand at 652.823 kb on - strand at 652.823 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 650,331 - rbsB MCAODC_03230 0.10 +0.5 650,444 - rbsB MCAODC_03230 0.22 -0.6 650,471 + rbsB MCAODC_03230 0.25 +0.2 650,472 - rbsB MCAODC_03230 0.25 -2.8 650,472 - rbsB MCAODC_03230 0.25 +1.6 650,505 + rbsB MCAODC_03230 0.29 -0.8 650,592 - rbsB MCAODC_03230 0.38 +0.7 650,655 + rbsB MCAODC_03230 0.44 +0.7 650,655 + rbsB MCAODC_03230 0.44 +0.4 650,656 - rbsB MCAODC_03230 0.45 -1.0 650,656 - rbsB MCAODC_03230 0.45 +0.0 650,659 + rbsB MCAODC_03230 0.45 +0.0 650,659 + rbsB MCAODC_03230 0.45 -0.6 650,659 + rbsB MCAODC_03230 0.45 -0.5 650,659 + rbsB MCAODC_03230 0.45 -0.8 650,659 + rbsB MCAODC_03230 0.45 +0.3 650,659 + rbsB MCAODC_03230 0.45 +1.0 650,659 + rbsB MCAODC_03230 0.45 +0.4 650,660 - rbsB MCAODC_03230 0.45 -3.5 650,660 - rbsB MCAODC_03230 0.45 -0.2 650,660 - rbsB MCAODC_03230 0.45 +0.4 650,660 - rbsB MCAODC_03230 0.45 -0.3 650,660 - rbsB MCAODC_03230 0.45 -0.4 650,660 - rbsB MCAODC_03230 0.45 +0.1 650,660 - rbsB MCAODC_03230 0.45 -0.3 650,756 - rbsB MCAODC_03230 0.55 -0.0 650,834 + rbsB MCAODC_03230 0.63 +0.4 650,834 + rbsB MCAODC_03230 0.63 -2.9 650,834 + rbsB MCAODC_03230 0.63 +1.3 650,835 - rbsB MCAODC_03230 0.64 +0.2 650,876 + rbsB MCAODC_03230 0.68 +0.4 650,876 + rbsB MCAODC_03230 0.68 +0.0 650,938 - rbsB MCAODC_03230 0.75 +1.2 650,949 + rbsB MCAODC_03230 0.76 -0.2 651,154 + -1.0 651,155 - -0.5 651,155 - -0.8 651,175 + -0.2 651,175 + -0.0 651,176 - +0.4 651,246 + +0.6 651,247 - -0.2 651,248 + -0.3 651,248 + +0.1 651,267 + +1.0 651,267 + +0.1 651,268 - -0.1 651,296 + +0.7 651,296 + -0.3 651,297 - +0.3 651,297 - -0.2 651,297 - -0.5 651,444 + ydaE MCAODC_03235 0.31 -1.5 651,478 + ydaE MCAODC_03235 0.36 -0.4 651,479 - ydaE MCAODC_03235 0.36 -0.8 651,479 - ydaE MCAODC_03235 0.36 -0.3 651,479 - ydaE MCAODC_03235 0.36 +0.4 651,552 + ydaE MCAODC_03235 0.47 +0.1 651,552 + ydaE MCAODC_03235 0.47 -0.0 651,585 + ydaE MCAODC_03235 0.52 +1.5 651,586 - ydaE MCAODC_03235 0.52 +1.1 651,591 - ydaE MCAODC_03235 0.52 +2.0 651,591 - ydaE MCAODC_03235 0.52 +0.1 651,618 + ydaE MCAODC_03235 0.56 -0.3 651,618 + ydaE MCAODC_03235 0.56 -0.6 651,619 - ydaE MCAODC_03235 0.57 -1.1 651,656 + ydaE MCAODC_03235 0.62 +0.3 651,656 + ydaE MCAODC_03235 0.62 -2.0 651,656 + ydaE MCAODC_03235 0.62 +1.8 651,656 + ydaE MCAODC_03235 0.62 +0.4 651,657 - ydaE MCAODC_03235 0.62 -0.9 651,678 + ydaE MCAODC_03235 0.65 +0.9 651,679 - ydaE MCAODC_03235 0.65 -0.5 651,679 - ydaE MCAODC_03235 0.65 +0.4 651,679 - ydaE MCAODC_03235 0.65 +0.3 651,717 + ydaE MCAODC_03235 0.71 -0.2 651,717 + ydaE MCAODC_03235 0.71 -0.0 651,718 - ydaE MCAODC_03235 0.71 -0.0 651,739 + ydaE MCAODC_03235 0.74 +1.5 651,740 - ydaE MCAODC_03235 0.74 -0.8 651,848 - +0.1 651,884 + +0.2 651,885 - -0.3 651,930 - -3.6 651,982 - +0.1 652,064 + fba MCAODC_03240 0.16 +0.4 652,064 + fba MCAODC_03240 0.16 +0.7 652,084 + fba MCAODC_03240 0.18 -0.1 652,085 - fba MCAODC_03240 0.18 -0.3 652,085 - fba MCAODC_03240 0.18 +0.2 652,091 + fba MCAODC_03240 0.19 +1.2 652,095 + fba MCAODC_03240 0.19 -0.1 652,095 + fba MCAODC_03240 0.19 -0.1 652,097 + fba MCAODC_03240 0.19 -1.1 652,097 + fba MCAODC_03240 0.19 -0.6 652,165 + fba MCAODC_03240 0.27 -2.2 652,166 - fba MCAODC_03240 0.27 +0.5 652,257 + fba MCAODC_03240 0.38 +0.8 652,369 - fba MCAODC_03240 0.51 -0.8 652,369 - fba MCAODC_03240 0.51 -0.8 652,480 - fba MCAODC_03240 0.64 +0.8 652,549 + fba MCAODC_03240 0.72 -1.4 652,549 + fba MCAODC_03240 0.72 -1.0 652,550 - fba MCAODC_03240 0.72 -0.1 652,552 + fba MCAODC_03240 0.72 +0.9 652,552 + fba MCAODC_03240 0.72 -0.5 652,553 - fba MCAODC_03240 0.72 +0.5 652,553 - fba MCAODC_03240 0.72 -0.8 652,571 + fba MCAODC_03240 0.74 -0.6 652,626 + fba MCAODC_03240 0.81 +0.6 652,626 + fba MCAODC_03240 0.81 +0.5 652,692 + fba MCAODC_03240 0.89 +0.3 652,693 - fba MCAODC_03240 0.89 -1.0 652,798 + +1.8 652,822 + +0.6 652,822 + -1.1 652,823 - -2.6 652,823 - +0.4 652,823 - +0.4
Or see this region's nucleotide sequence