Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hsdM and hsdS overlap by 1 nucleotides hsdS and symE are separated by 220 nucleotides symE and MCAODC_01950 are separated by 46 nucleotides
MCAODC_01930: hsdM - Type I restriction enzyme EcoEI methylase subunit, at 374,565 to 376,034
hsdM
MCAODC_01935: hsdS - restriction endonuclease subunit S, at 376,034 to 377,788
hsdS
MCAODC_01945: symE - endoribonuclease SymE, at 378,009 to 378,350
symE
MCAODC_01950: MCAODC_01950 - DUF1524 domain-containing protein, at 378,397 to 379,560
_01950
Position (kb) 376
377
378 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 375.058 kb on - strand, within hsdM at 375.060 kb on - strand, within hsdM at 375.083 kb on + strand, within hsdM at 375.083 kb on + strand, within hsdM at 375.122 kb on + strand, within hsdM at 375.175 kb on + strand, within hsdM at 375.176 kb on - strand, within hsdM at 375.176 kb on - strand, within hsdM at 375.176 kb on - strand, within hsdM at 375.187 kb on - strand, within hsdM at 375.278 kb on + strand, within hsdM at 375.278 kb on + strand, within hsdM at 375.279 kb on - strand, within hsdM at 375.279 kb on - strand, within hsdM at 375.279 kb on - strand, within hsdM at 375.377 kb on + strand, within hsdM at 375.488 kb on - strand, within hsdM at 375.488 kb on - strand, within hsdM at 375.536 kb on - strand, within hsdM at 375.536 kb on - strand, within hsdM at 375.536 kb on - strand, within hsdM at 375.571 kb on + strand, within hsdM at 375.596 kb on + strand, within hsdM at 375.596 kb on + strand, within hsdM at 375.699 kb on + strand, within hsdM at 375.699 kb on + strand, within hsdM at 375.699 kb on + strand, within hsdM at 375.700 kb on - strand, within hsdM at 375.700 kb on - strand, within hsdM at 375.725 kb on - strand, within hsdM at 375.887 kb on + strand, within hsdM at 375.887 kb on + strand, within hsdM at 375.945 kb on + strand at 375.946 kb on - strand at 375.946 kb on - strand at 376.029 kb on - strand at 376.029 kb on - strand at 376.032 kb on + strand at 376.032 kb on + strand at 376.032 kb on + strand at 376.032 kb on + strand at 376.032 kb on + strand at 376.033 kb on - strand at 376.134 kb on + strand at 376.135 kb on - strand at 376.135 kb on - strand at 376.135 kb on - strand at 376.135 kb on - strand at 376.135 kb on - strand at 376.136 kb on + strand at 376.136 kb on + strand at 376.186 kb on + strand at 376.309 kb on + strand, within hsdS at 376.360 kb on + strand, within hsdS at 376.360 kb on + strand, within hsdS at 376.360 kb on + strand, within hsdS at 376.361 kb on - strand, within hsdS at 376.412 kb on - strand, within hsdS at 376.659 kb on - strand, within hsdS at 376.691 kb on + strand, within hsdS at 376.692 kb on - strand, within hsdS at 376.816 kb on - strand, within hsdS at 376.949 kb on - strand, within hsdS at 376.959 kb on - strand, within hsdS at 376.971 kb on + strand, within hsdS at 376.971 kb on + strand, within hsdS at 376.972 kb on - strand, within hsdS at 376.972 kb on - strand, within hsdS at 376.972 kb on - strand, within hsdS at 377.029 kb on - strand, within hsdS at 377.277 kb on + strand, within hsdS at 377.292 kb on + strand, within hsdS at 377.426 kb on - strand, within hsdS at 377.441 kb on - strand, within hsdS at 377.441 kb on - strand, within hsdS at 377.445 kb on + strand, within hsdS at 377.462 kb on + strand, within hsdS at 377.463 kb on - strand, within hsdS at 377.465 kb on - strand, within hsdS at 377.539 kb on + strand, within hsdS at 377.700 kb on + strand at 377.701 kb on - strand at 377.701 kb on - strand at 377.878 kb on - strand at 377.923 kb on + strand at 377.924 kb on - strand at 377.988 kb on + strand at 377.994 kb on + strand at 378.001 kb on + strand at 378.008 kb on + strand at 378.181 kb on + strand, within symE at 378.182 kb on - strand, within symE at 378.182 kb on - strand, within symE at 378.206 kb on + strand, within symE at 378.206 kb on + strand, within symE at 378.207 kb on - strand, within symE at 378.291 kb on - strand, within symE at 378.351 kb on + strand at 378.351 kb on + strand at 378.400 kb on + strand at 378.401 kb on - strand at 378.443 kb on - strand at 378.455 kb on + strand at 378.456 kb on - strand at 378.571 kb on - strand, within MCAODC_01950 at 378.573 kb on + strand, within MCAODC_01950 at 378.573 kb on + strand, within MCAODC_01950 at 378.574 kb on - strand, within MCAODC_01950 at 378.642 kb on + strand, within MCAODC_01950 at 378.642 kb on + strand, within MCAODC_01950 at 378.643 kb on - strand, within MCAODC_01950 at 378.643 kb on - strand, within MCAODC_01950 at 378.643 kb on - strand, within MCAODC_01950 at 378.644 kb on + strand, within MCAODC_01950 at 378.644 kb on + strand, within MCAODC_01950 at 378.645 kb on - strand, within MCAODC_01950 at 378.645 kb on - strand, within MCAODC_01950 at 378.645 kb on - strand, within MCAODC_01950 at 378.750 kb on - strand, within MCAODC_01950 at 378.765 kb on - strand, within MCAODC_01950
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 375,058 - hsdM MCAODC_01930 0.34 -1.2 375,060 - hsdM MCAODC_01930 0.34 +1.2 375,083 + hsdM MCAODC_01930 0.35 -1.3 375,083 + hsdM MCAODC_01930 0.35 +3.0 375,122 + hsdM MCAODC_01930 0.38 -0.8 375,175 + hsdM MCAODC_01930 0.41 +0.8 375,176 - hsdM MCAODC_01930 0.42 -1.2 375,176 - hsdM MCAODC_01930 0.42 -0.6 375,176 - hsdM MCAODC_01930 0.42 +0.2 375,187 - hsdM MCAODC_01930 0.42 +0.3 375,278 + hsdM MCAODC_01930 0.49 -1.2 375,278 + hsdM MCAODC_01930 0.49 -0.1 375,279 - hsdM MCAODC_01930 0.49 -0.6 375,279 - hsdM MCAODC_01930 0.49 +0.9 375,279 - hsdM MCAODC_01930 0.49 -0.9 375,377 + hsdM MCAODC_01930 0.55 +0.5 375,488 - hsdM MCAODC_01930 0.63 -2.5 375,488 - hsdM MCAODC_01930 0.63 +0.8 375,536 - hsdM MCAODC_01930 0.66 -0.4 375,536 - hsdM MCAODC_01930 0.66 -1.0 375,536 - hsdM MCAODC_01930 0.66 -0.0 375,571 + hsdM MCAODC_01930 0.68 -0.3 375,596 + hsdM MCAODC_01930 0.70 +0.8 375,596 + hsdM MCAODC_01930 0.70 +0.4 375,699 + hsdM MCAODC_01930 0.77 +0.3 375,699 + hsdM MCAODC_01930 0.77 +0.9 375,699 + hsdM MCAODC_01930 0.77 +1.5 375,700 - hsdM MCAODC_01930 0.77 +0.0 375,700 - hsdM MCAODC_01930 0.77 -1.1 375,725 - hsdM MCAODC_01930 0.79 +2.6 375,887 + hsdM MCAODC_01930 0.90 +0.6 375,887 + hsdM MCAODC_01930 0.90 +0.6 375,945 + +0.8 375,946 - -0.4 375,946 - -0.7 376,029 - +0.2 376,029 - +0.3 376,032 + +0.9 376,032 + -0.0 376,032 + +0.9 376,032 + -0.0 376,032 + -1.9 376,033 - +0.6 376,134 + +0.7 376,135 - -3.5 376,135 - +1.3 376,135 - -1.3 376,135 - +0.2 376,135 - +2.1 376,136 + -1.3 376,136 + +1.2 376,186 + +0.9 376,309 + hsdS MCAODC_01935 0.16 +0.7 376,360 + hsdS MCAODC_01935 0.19 +0.2 376,360 + hsdS MCAODC_01935 0.19 +0.6 376,360 + hsdS MCAODC_01935 0.19 -2.2 376,361 - hsdS MCAODC_01935 0.19 -2.4 376,412 - hsdS MCAODC_01935 0.22 -0.8 376,659 - hsdS MCAODC_01935 0.36 -0.1 376,691 + hsdS MCAODC_01935 0.37 -1.2 376,692 - hsdS MCAODC_01935 0.37 +1.0 376,816 - hsdS MCAODC_01935 0.45 +0.6 376,949 - hsdS MCAODC_01935 0.52 +0.1 376,959 - hsdS MCAODC_01935 0.53 +1.2 376,971 + hsdS MCAODC_01935 0.53 -0.3 376,971 + hsdS MCAODC_01935 0.53 -0.2 376,972 - hsdS MCAODC_01935 0.53 +3.3 376,972 - hsdS MCAODC_01935 0.53 -0.4 376,972 - hsdS MCAODC_01935 0.53 -0.8 377,029 - hsdS MCAODC_01935 0.57 -3.9 377,277 + hsdS MCAODC_01935 0.71 -1.1 377,292 + hsdS MCAODC_01935 0.72 +0.1 377,426 - hsdS MCAODC_01935 0.79 -0.3 377,441 - hsdS MCAODC_01935 0.80 -2.1 377,441 - hsdS MCAODC_01935 0.80 -3.1 377,445 + hsdS MCAODC_01935 0.80 +2.3 377,462 + hsdS MCAODC_01935 0.81 +2.1 377,463 - hsdS MCAODC_01935 0.81 +1.3 377,465 - hsdS MCAODC_01935 0.82 +0.8 377,539 + hsdS MCAODC_01935 0.86 +0.3 377,700 + -0.0 377,701 - -1.3 377,701 - -1.4 377,878 - +0.3 377,923 + +0.3 377,924 - +0.6 377,988 + -0.5 377,994 + +0.2 378,001 + -0.5 378,008 + +0.6 378,181 + symE MCAODC_01945 0.50 -0.4 378,182 - symE MCAODC_01945 0.51 +0.8 378,182 - symE MCAODC_01945 0.51 -0.1 378,206 + symE MCAODC_01945 0.58 +3.0 378,206 + symE MCAODC_01945 0.58 +0.5 378,207 - symE MCAODC_01945 0.58 -1.3 378,291 - symE MCAODC_01945 0.82 +0.8 378,351 + -0.2 378,351 + +1.8 378,400 + -0.1 378,401 - -1.7 378,443 - -0.3 378,455 + +0.5 378,456 - -2.4 378,571 - MCAODC_01950 0.15 +0.5 378,573 + MCAODC_01950 0.15 +1.4 378,573 + MCAODC_01950 0.15 -0.2 378,574 - MCAODC_01950 0.15 -0.0 378,642 + MCAODC_01950 0.21 +0.7 378,642 + MCAODC_01950 0.21 -2.0 378,643 - MCAODC_01950 0.21 +0.4 378,643 - MCAODC_01950 0.21 -0.0 378,643 - MCAODC_01950 0.21 -0.4 378,644 + MCAODC_01950 0.21 -0.5 378,644 + MCAODC_01950 0.21 +1.6 378,645 - MCAODC_01950 0.21 +2.0 378,645 - MCAODC_01950 0.21 -1.2 378,645 - MCAODC_01950 0.21 -0.5 378,750 - MCAODC_01950 0.30 -1.3 378,765 - MCAODC_01950 0.32 +0.8
Or see this region's nucleotide sequence