Strain Fitness in Escherichia coli ECRC101 around MCAODC_00245

Experiment: WM_Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyafP and yafO are separated by 84 nucleotidesyafO and yafN are separated by 2 nucleotidesyafN and dinB are separated by 51 nucleotides MCAODC_00235: yafP - GNAT family N-acetyltransferase, at 23,547 to 23,924 yafP MCAODC_00240: yafO - type II toxin-antitoxin system mRNA interferase toxin YafO, at 24,009 to 24,407 yafO MCAODC_00245: yafN - type I toxin-antitoxin system antitoxin YafN, at 24,410 to 24,703 yafN MCAODC_00250: dinB - DNA polymerase IV, at 24,755 to 25,810 dinB Position (kb) 24 25Strain fitness (log2 ratio) -2 -1 0 1 2at 23.563 kb on + strandat 23.619 kb on - strand, within yafPat 23.619 kb on - strand, within yafPat 23.679 kb on - strand, within yafPat 23.696 kb on + strand, within yafPat 23.769 kb on - strand, within yafPat 23.769 kb on - strand, within yafPat 23.770 kb on + strand, within yafPat 23.950 kb on - strandat 23.975 kb on - strandat 24.051 kb on - strand, within yafOat 24.145 kb on + strand, within yafOat 24.146 kb on - strand, within yafOat 24.146 kb on - strand, within yafOat 24.228 kb on - strand, within yafOat 24.297 kb on + strand, within yafOat 24.297 kb on + strand, within yafOat 24.298 kb on - strand, within yafOat 24.298 kb on - strand, within yafOat 24.326 kb on - strand, within yafOat 24.419 kb on - strandat 24.703 kb on + strandat 24.703 kb on + strandat 24.719 kb on + strandat 24.743 kb on + strandat 24.744 kb on - strandat 24.746 kb on + strandat 24.747 kb on - strandat 24.749 kb on + strandat 24.749 kb on + strandat 24.751 kb on + strandat 24.751 kb on + strandat 24.875 kb on + strand, within dinBat 24.980 kb on + strand, within dinBat 24.980 kb on + strand, within dinBat 25.054 kb on + strand, within dinBat 25.136 kb on - strand, within dinBat 25.342 kb on + strand, within dinBat 25.494 kb on + strand, within dinBat 25.495 kb on - strand, within dinBat 25.653 kb on - strand, within dinBat 25.675 kb on - strand, within dinB

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas09
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23,563 + +0.6
23,619 - yafP MCAODC_00235 0.19 -1.0
23,619 - yafP MCAODC_00235 0.19 +0.8
23,679 - yafP MCAODC_00235 0.35 -0.2
23,696 + yafP MCAODC_00235 0.39 +0.2
23,769 - yafP MCAODC_00235 0.59 +0.0
23,769 - yafP MCAODC_00235 0.59 +0.5
23,770 + yafP MCAODC_00235 0.59 -1.5
23,950 - -0.1
23,975 - +0.2
24,051 - yafO MCAODC_00240 0.11 +0.2
24,145 + yafO MCAODC_00240 0.34 +0.9
24,146 - yafO MCAODC_00240 0.34 +0.5
24,146 - yafO MCAODC_00240 0.34 -1.1
24,228 - yafO MCAODC_00240 0.55 -0.0
24,297 + yafO MCAODC_00240 0.72 +0.1
24,297 + yafO MCAODC_00240 0.72 +0.7
24,298 - yafO MCAODC_00240 0.72 +1.6
24,298 - yafO MCAODC_00240 0.72 +1.1
24,326 - yafO MCAODC_00240 0.79 +2.1
24,419 - +1.7
24,703 + +0.1
24,703 + -1.6
24,719 + -0.7
24,743 + -0.6
24,744 - -2.0
24,746 + -1.4
24,747 - +0.3
24,749 + -2.1
24,749 + +0.4
24,751 + -1.4
24,751 + -0.5
24,875 + dinB MCAODC_00250 0.11 -1.5
24,980 + dinB MCAODC_00250 0.21 +1.4
24,980 + dinB MCAODC_00250 0.21 +1.0
25,054 + dinB MCAODC_00250 0.28 +0.2
25,136 - dinB MCAODC_00250 0.36 -0.3
25,342 + dinB MCAODC_00250 0.56 +0.5
25,494 + dinB MCAODC_00250 0.70 +0.4
25,495 - dinB MCAODC_00250 0.70 +0.7
25,653 - dinB MCAODC_00250 0.85 -0.5
25,675 - dinB MCAODC_00250 0.87 -0.7

Or see this region's nucleotide sequence