Strain Fitness in Escherichia coli ECRC101 around MCAODC_23205

Experiment: WM_Bas09

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcsgB and cgsD are separated by 753 nucleotidescgsD and cgsE are separated by 4 nucleotidescgsE and cgsF are separated by 24 nucleotidescgsF and cgsG are separated by 26 nucleotides MCAODC_23195: csgB - curlin minor subunit CsgB, at 1,376,934 to 1,377,389 csgB MCAODC_23205: cgsD - transcriptional regulator CsgD, at 1,378,143 to 1,378,793 cgsD MCAODC_23210: cgsE - curli production assembly/transport protein CsgE, at 1,378,798 to 1,379,187 cgsE MCAODC_23215: cgsF - curli production assembly/transport protein CsgF, at 1,379,212 to 1,379,628 cgsF MCAODC_23220: cgsG - curli production assembly/transport protein CsgG, at 1,379,655 to 1,380,488 cgsG Position (kb) 1378 1379Strain fitness (log2 ratio) -2 -1 0 1at 1377.165 kb on - strand, within csgBat 1377.225 kb on - strand, within csgBat 1377.309 kb on - strand, within csgBat 1377.355 kb on - strandat 1377.492 kb on - strandat 1377.686 kb on + strandat 1378.001 kb on + strandat 1378.047 kb on + strandat 1378.047 kb on + strandat 1378.074 kb on + strandat 1378.149 kb on - strandat 1378.402 kb on + strand, within cgsDat 1378.579 kb on - strand, within cgsDat 1378.584 kb on + strand, within cgsDat 1378.587 kb on - strand, within cgsDat 1378.980 kb on + strand, within cgsEat 1378.980 kb on + strand, within cgsEat 1379.232 kb on - strandat 1379.237 kb on + strandat 1379.510 kb on - strand, within cgsFat 1379.511 kb on + strand, within cgsFat 1379.520 kb on + strand, within cgsF

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Bas09
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1,377,165 - csgB MCAODC_23195 0.51 -0.2
1,377,225 - csgB MCAODC_23195 0.64 +1.2
1,377,309 - csgB MCAODC_23195 0.82 +0.6
1,377,355 - -2.0
1,377,492 - -2.0
1,377,686 + -0.3
1,378,001 + +1.4
1,378,047 + +0.1
1,378,047 + +0.3
1,378,074 + +0.0
1,378,149 - -0.5
1,378,402 + cgsD MCAODC_23205 0.40 +0.6
1,378,579 - cgsD MCAODC_23205 0.67 +0.8
1,378,584 + cgsD MCAODC_23205 0.68 +1.3
1,378,587 - cgsD MCAODC_23205 0.68 +0.4
1,378,980 + cgsE MCAODC_23210 0.47 +0.7
1,378,980 + cgsE MCAODC_23210 0.47 +1.2
1,379,232 - -0.7
1,379,237 + +0.9
1,379,510 - cgsF MCAODC_23215 0.71 -0.0
1,379,511 + cgsF MCAODC_23215 0.72 +0.6
1,379,520 + cgsF MCAODC_23215 0.74 -1.0

Or see this region's nucleotide sequence