Experiment: WM_Bas09
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ibsA and MCAODC_20725 are separated by 271 nucleotides MCAODC_20725 and MCAODC_20735 are separated by 271 nucleotides MCAODC_20735 and mdtA are separated by 279 nucleotides mdtA and mdtB overlap by 1 nucleotides
MCAODC_20715: ibsA - Small toxic protein IbsA, at 956,344 to 956,403
ibsA
MCAODC_20725: MCAODC_20725 - Type I toxin-antitoxin system Ibs family toxin, at 956,675 to 956,731
_20725
MCAODC_20735: MCAODC_20735 - Type I toxin-antitoxin system Ibs family toxin, at 957,003 to 957,059
_20735
MCAODC_20740: mdtA - multidrug efflux RND transporter subunit MdtA, at 957,339 to 958,586
mdtA
MCAODC_20745: mdtB - multidrug efflux RND transporter permease subunit MdtB, at 958,586 to 961,708
mdtB
Position (kb) 957
958
959 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 956.345 kb on + strand at 956.345 kb on + strand at 956.345 kb on + strand at 956.346 kb on - strand at 956.349 kb on - strand at 956.349 kb on - strand at 956.361 kb on + strand, within ibsA at 956.362 kb on - strand, within ibsA at 956.369 kb on - strand, within ibsA at 956.374 kb on + strand, within ibsA at 956.374 kb on + strand, within ibsA at 956.383 kb on + strand, within ibsA at 956.384 kb on - strand, within ibsA at 956.404 kb on - strand at 956.567 kb on - strand at 956.670 kb on + strand at 956.679 kb on + strand at 956.693 kb on + strand, within MCAODC_20725 at 956.693 kb on + strand, within MCAODC_20725 at 956.693 kb on + strand, within MCAODC_20725 at 956.694 kb on - strand, within MCAODC_20725 at 956.699 kb on + strand, within MCAODC_20725 at 956.748 kb on - strand at 956.748 kb on - strand at 956.998 kb on + strand at 956.998 kb on + strand at 957.004 kb on + strand at 957.004 kb on + strand at 957.004 kb on + strand at 957.007 kb on + strand at 957.007 kb on + strand at 957.017 kb on - strand, within MCAODC_20735 at 957.020 kb on + strand, within MCAODC_20735 at 957.048 kb on + strand, within MCAODC_20735 at 957.049 kb on - strand, within MCAODC_20735 at 957.071 kb on + strand at 957.075 kb on + strand at 957.135 kb on + strand at 957.178 kb on + strand at 957.258 kb on - strand at 957.291 kb on - strand at 957.291 kb on - strand at 957.295 kb on + strand at 957.295 kb on + strand at 957.296 kb on - strand at 957.299 kb on - strand at 957.299 kb on - strand at 957.353 kb on + strand at 957.354 kb on - strand at 957.354 kb on - strand at 957.354 kb on - strand at 957.382 kb on + strand at 957.514 kb on + strand, within mdtA at 957.594 kb on - strand, within mdtA at 957.596 kb on + strand, within mdtA at 957.596 kb on + strand, within mdtA at 957.596 kb on + strand, within mdtA at 957.679 kb on + strand, within mdtA at 957.876 kb on - strand, within mdtA at 958.038 kb on - strand, within mdtA at 958.118 kb on - strand, within mdtA at 958.150 kb on + strand, within mdtA at 958.208 kb on + strand, within mdtA at 958.209 kb on - strand, within mdtA at 958.226 kb on + strand, within mdtA at 958.227 kb on - strand, within mdtA at 958.279 kb on + strand, within mdtA at 958.633 kb on + strand at 958.633 kb on + strand at 958.634 kb on - strand at 958.636 kb on + strand at 958.882 kb on + strand at 958.890 kb on + strand at 958.891 kb on - strand at 958.891 kb on - strand at 959.080 kb on + strand, within mdtB at 959.252 kb on - strand, within mdtB at 959.343 kb on + strand, within mdtB at 959.343 kb on + strand, within mdtB at 959.397 kb on + strand, within mdtB at 959.397 kb on + strand, within mdtB at 959.486 kb on - strand, within mdtB at 959.491 kb on + strand, within mdtB at 959.492 kb on - strand, within mdtB at 959.581 kb on + strand, within mdtB
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Bas09 remove 956,345 + +1.3 956,345 + +0.4 956,345 + -0.5 956,346 - -0.7 956,349 - +0.1 956,349 - -0.1 956,361 + ibsA MCAODC_20715 0.28 +0.0 956,362 - ibsA MCAODC_20715 0.30 +0.9 956,369 - ibsA MCAODC_20715 0.42 -0.7 956,374 + ibsA MCAODC_20715 0.50 -0.2 956,374 + ibsA MCAODC_20715 0.50 -0.1 956,383 + ibsA MCAODC_20715 0.65 +0.2 956,384 - ibsA MCAODC_20715 0.67 +0.3 956,404 - +0.1 956,567 - -1.0 956,670 + -0.7 956,679 + -0.2 956,693 + MCAODC_20725 0.32 +0.2 956,693 + MCAODC_20725 0.32 -0.4 956,693 + MCAODC_20725 0.32 +2.6 956,694 - MCAODC_20725 0.33 -1.9 956,699 + MCAODC_20725 0.42 +0.3 956,748 - -0.5 956,748 - +0.1 956,998 + -0.2 956,998 + +0.1 957,004 + -0.2 957,004 + +0.0 957,004 + -1.4 957,007 + +0.2 957,007 + -1.1 957,017 - MCAODC_20735 0.25 +0.8 957,020 + MCAODC_20735 0.30 +0.1 957,048 + MCAODC_20735 0.79 -0.4 957,049 - MCAODC_20735 0.81 +0.7 957,071 + +0.7 957,075 + +0.1 957,135 + -0.3 957,178 + -2.1 957,258 - -0.3 957,291 - -0.0 957,291 - -0.3 957,295 + +0.8 957,295 + -0.7 957,296 - +1.2 957,299 - +0.4 957,299 - +0.1 957,353 + -1.0 957,354 - -1.3 957,354 - +0.1 957,354 - +2.1 957,382 + +0.7 957,514 + mdtA MCAODC_20740 0.14 -0.4 957,594 - mdtA MCAODC_20740 0.20 +0.2 957,596 + mdtA MCAODC_20740 0.21 +0.4 957,596 + mdtA MCAODC_20740 0.21 +0.3 957,596 + mdtA MCAODC_20740 0.21 +1.2 957,679 + mdtA MCAODC_20740 0.27 -0.8 957,876 - mdtA MCAODC_20740 0.43 -0.9 958,038 - mdtA MCAODC_20740 0.56 -0.4 958,118 - mdtA MCAODC_20740 0.62 +0.1 958,150 + mdtA MCAODC_20740 0.65 +0.9 958,208 + mdtA MCAODC_20740 0.70 +1.0 958,209 - mdtA MCAODC_20740 0.70 -1.0 958,226 + mdtA MCAODC_20740 0.71 -0.3 958,227 - mdtA MCAODC_20740 0.71 -0.4 958,279 + mdtA MCAODC_20740 0.75 -0.6 958,633 + -2.9 958,633 + -0.7 958,634 - +0.3 958,636 + -0.5 958,882 + +0.5 958,890 + +0.1 958,891 - +0.2 958,891 - -0.4 959,080 + mdtB MCAODC_20745 0.16 -1.9 959,252 - mdtB MCAODC_20745 0.21 -0.3 959,343 + mdtB MCAODC_20745 0.24 -0.7 959,343 + mdtB MCAODC_20745 0.24 +0.0 959,397 + mdtB MCAODC_20745 0.26 -1.7 959,397 + mdtB MCAODC_20745 0.26 -2.6 959,486 - mdtB MCAODC_20745 0.29 +0.2 959,491 + mdtB MCAODC_20745 0.29 +2.5 959,492 - mdtB MCAODC_20745 0.29 +0.3 959,581 + mdtB MCAODC_20745 0.32 -0.1
Or see this region's nucleotide sequence